| Project/Area Number |
18613021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
IMBE Hiroki Wakayama Medical University, 医学部, Lecturer (60326353)
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| Co-Investigator(Kenkyū-buntansha) |
AIKAWA Fumiko Osaka Dental University, 歯学部, Assistant (60411427)
SENBA Emiko Wakayama Medical University, 医学部, Professor (00135691)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,240,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Signal transduction / Pain |
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| Research Abstract |
1. The significance of ERK activation in the rostral ventromedial medulla (RVM) during peripheral inflammation : the microinjection of the drug into the brain.
Microinjection of U0126, a mitogen-activated protein kinase kinase inhibitor, into the RVM decreased phosphorylated ERK at 7 h after complete Freund's adjuvant (CFA) injection into the rat hindpaw. The U0126 microinjection also attenuated thermal hyperalgesia in the ipsilateral hindpaw at 24 h after CFA injection. These findings showed that activation of ERK in the RVM contributed to thermal hyperalgesia during peripheral inflammation.
2. The expression of p-p38 mitogen-activated protein kinase (MAPK) in the RVM during peripheral inflammation.
At 30 min after CFA injection into the rat hindpaw, the number of p-p38 MAPK-IR neurons in the RVM was significantly higher than that in the naive group, which lasted for 1 h. At 30 min after CFA injection, about 70% of serotonergic neurons (tryptophan hydroxylase, TPH, positive) in the RVM were p-p 38 MAPK positive. The number of p-p38 MAPK- and TPH-double-positive RVM neurons in the rats with inflammation was significantly higher than that in naive rats. These findings showed that p38 MAPK was activated in the RVM serotonergic neuron at the early time point of peripheral inflammation.
3. The expression of p-ERK in the locus coeruleus (LC) during peripheral inflammation.
At 5 min after formalin injection into the rat hindpaw, the number of p-ERK-IR neurons in the LC was significantly higher than that in the naive group, which lasted for 1 h. These findings showed that ERK was activated in the LC following acute noxious stimulation.
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