草本リグニンと木本リグニンを区別する鍵構造としてのエリトロ型β-0-4構造

• Project/Area Number: 18658068
• Research Category: Grant-in-Aid for Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: 林産科学・木質工学
• Research Institution: The University of Tokyo
• Principal Investigator: 松本 雄二 The University of Tokyo, 大学院・農学生命科学研究科, 教授 (30183619)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2008: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: リグニン / 細胞壁 / NMR / 木粉溶液 / エリトロ / クチン / 草本 / 赤外スペクトル / 木化 / 木本 / 立体構造

Research Abstract

昨年度までの研究により、非木質試料(草本類や葉)と木本類試料では、リグニン構造分析における指標分解物(ニトロベンゼン酸化における芳香族アルデヒド類、オゾン分解におけるテトロン酸類など)の、試料あたりの収率がはっきりと異なることを明らかにすることができた。特にエリトロ型β-O-4構造のオゾン分解生成物であるエリトロン酸の細胞壁当りの収率によって、非木質試料と木本類試料をはっきりと区別することができた。一方、リグニン量と言う観点からこれら試料の差を分析しようとすると、クラーソン法を用いる限り、木本と草本の相違、堅い試料と柔らかい試料の相違を明らかにすることができなかった。このことは、草本類におけるリグニン分析にはクラーソン法が適用できないことを示しており、クラーソン法によって草本や葉などのリグニン量を評価すべきでないことを意味している。今年度は、クラーソン法の適用できないこれらの試料のリグニン量の評価のために、2つの新しい方法を適用した。一つ目は、赤外スペクトルを用いてリグニン量を評価することであり、2つ目は、昨年度申請者が開発した植物細胞壁の全溶解法をもちいてNMR的にリグニン量を評価することである。このうち、赤外スペクトルによるリグニン量の評価は、上記の指標化合物の収率ときわめて良い相関を示した。また赤外スペクトルの分析から、葉などの試料ではクチン質の存在が、クラーソンリグニン法が過大なリグニン量を与える原因の一つとなっていることが示された。非木質試料の試料調製過程において前処理として行われる各種の前抽出法が、この物質を除く上で必ずしも効果が無いことも明らかになった。

Research Products

• [Journal Article] The Relationship between Lignin and Morphological Characteristics of the Tracheary Elements from Cacao (Theobroma cacao L.) Hulls (2008)
  • Author(s): Byung Yeoup Chung, Jea-Young Cho, Seung Sik Lee, Yoshiharu Nishiyama, Yuji Matsumoto, Kenji Iiyama
  • Journal Title: Journal od Plant Biology 52(2) Pages: 139-144
  • Peer Reviewed
• [Journal Article] The Relationship between Lignin and Morphological Characteristics of the Tracheary Elements from Cacao (Theobroma cacao L.) Hulls. (2008)
  • Author(s): Byung Yeoup Chung, Jae-Young Cho, Seung Sik Lee, Yoshiharu Nishiyama. Yuji Matsumoto, Kenji
  • Journal Title: Journal of Plant Biology 51(2) Pages: 139-144
  • Peer Reviewed
• [Journal Article] Reexamination of the mechanism of β-O-4 bond cleavage during acidolysis of lignin part 1:Kinetic of the exclusive formation of enol ether from non-phenolic C6-C2 type model compounds (2008)
  • Author(s): Tomoya Yokoyama, Yuji Matsumoto
  • Journal Title: Holzforschung 62(2) Pages: 164-168
  • Peer Reviewed
• [Journal Article] Significant Lability of Guaiacylglycerol-β-Phenacyl Ether under Alkaline Conditions (2007)
  • Author(s): Aiko Imai, Tomoya Yokoyama, Yuji Matsumoto, and Gyosuke Meshitsuka
  • Journal Title: J. Agric. Food Chem. 55(22) Pages: 9043-9046
  • Peer Reviewed
• [Journal Article] Structural characteristics of lignin in primitive pteridophytes: Selaginella species (2007)
  • Author(s): 6. Zhenfu Jin, Yuji Matsumoto, Takeshi Tange, Kenji Iiyama
  • Journal Title: J. Wood Science 53(5) Pages: 412-417
  • NAID: 10022024710
  • Peer Reviewed
• [Journal Article] Determination of the accessible hydroxyl groups in heat-treated Styrax tonkinensis (Pierre) Craib ex Hartwich wood by hydrogen-deuterium exchange and 2H NMR spectroscopy (2007)
  • Author(s): Le Xuan Phuong, Masato Takayama, Satoshi Shida, Yuji Matsumoto, Tetsuo Aoyagi
  • Journal Title: Holzforschung 61 Pages: 488-491
  • Peer Reviewed
• [Journal Article] Structural characteristics of lignin in primitive pteridophytes : Selaginella species. J. Wood Science (2007)
  • Author(s): Zhenfu Jin, Yuji Matsumoto, Takeshi Tange, Kenji liyama
  • Journal Title: J. Wood Science (Published online: 31 March)
• [Journal Article] Detailed Examination of the Degradation of Phenol Derivatives under Oxygen Delignification Conditions (2007)
  • Author(s): Tomoya Yokoyama, Yuji Matsumoto, Gyosuke Meshitsuka
  • Journal Title: Journal of Agricultural and Food Chemistry 55(4) Pages: 1301-1307
• [Journal Article] Structural difference between leaf blade and petiole of original and mulched leaf litter of Ginkgo biloba (2006)
  • Author(s): Zhenfu Jin, Yuji Matsumoto, Shunliu Shao, Takuya Akiyama, Kenji liyama
  • Journal Title: Bull. Tokyo Univ. For. 115 Pages: 51-64
• [Journal Article] Leaf litter decomposition of selected urban tree species during mulching (2006)
  • Author(s): Zhenfu Jin, Yuji Matsumoto, Shunliu Shao, Takuya Akiyama, Kenji liyama
  • Journal Title: Bull. Tokyo Univ. For. 115 Pages: 37-50
  • NAID: 120001092981
• [Journal Article] Elucidation of the structure of cellulolytic enzyme lignin (2006)
  • Author(s): Zhoujian Hu, Ting-Feng Yeh, Hou-min Chang, Yuji Matsumoto, John F. Kadla
  • Journal Title: Holzforschung 60 Pages: 389-397
• [Presentation] Studies on the effect of ball milling on lignin structure by the use of complete wood dissolution (2008)
  • Author(s): Zhiguo Wang, Tomoya Yokoyama, Hou-min Chang, Yuji Matsumoto
  • Organizer: International Conference on Pulping, Papermaking and Biotechnology 2008
  • Place of Presentation: Nanjing, China
  • Year and Date: 2008-11-04
• [Presentation] Lignin content in non-wood cell walls (2008)
  • Author(s): Hirotaka Nakamura, Tomoya yokoyama, Yuji matsumoto, Zhenfu Jin
  • Organizer: International Conference on Pulping, Papermaking and Biotechnology 2008
  • Place of Presentation: Nanjing, China
  • Year and Date: 2008-11-04
• [Presentation] Variation in the chemical structure of lignin in tropical wood species (2008)
  • Author(s): Deded Sarip Nawaei, Tomoya Yokoyama, Yuji Matsumoto, Wasrin Syafii, Iori Tomoda, Yosuke Uchida
  • Organizer: International Conference on Pulping, Papermaking and Biotechnology 2008
  • Place of Presentation: Nanjing, China
  • Year and Date: 2008-11-04
• [Presentation] Analysis of lignin by the use of whole wood solution consisting of DMSO-LiCl (2008)
  • Author(s): 王志国,横山朝哉,松本雄二
  • Organizer: 第53回リグニン討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-31
• [Presentation] 非木質組織のリグニン定量 (2008)
  • Author(s): 戸田守一,中村裕貴,横山朝哉,松本雄二
  • Organizer: 第53回リグニン討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-31
• [Presentation] Change of lignin structure during MWL isolation porocess (2008)
  • Author(s): Aya Fujimoto, Zhiguo Wang, Tomoya Yokoyama, Yuji Matsumoto, Gyosuke Meshitsuka, Hou-min Chang,
  • Organizer: International Symposium on Wood Science and Technology
  • Place of Presentation: Harbin, China
  • Year and Date: 2008-09-27
• [Presentation] Dissolution of finely milled wood into organic solvent and its potential application for chemical analysis of cell wall components (2008)
  • Author(s): Zhiguo Wang, Tomoya Yokoyama, Hou-min Chang, Yuji Matsumoto
  • Organizer: 10th European Workshop on Lignocellulosics and Pulp
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2008-08-25
• [Presentation] Dissolution of finely milled wood into organic solvent (2008)
  • Author(s): Wang Thiguo, Tomoya Yokoyama, Yuji Matsumoto
  • Organizer: 第58回日本木材学会大会
  • Place of Presentation: つくば
  • Year and Date: 2008-03-19
• [Presentation] 非木質試料におけるリグニン定量値の評価 (2007)
  • Author(s): 中村裕貴, 秋山拓也, Zhenfu Jin, Deded S. Nawawi, 横山朝哉, 松本雄二
  • Organizer: 第52回リグニン討論会, 2007, 宇都宮
  • Place of Presentation: 宇都宮
  • Year and Date: 2007-11-14
• [Presentation] Variety of lignin structure in angiosperms and their reaction wood (2007)
  • Author(s): Yuji Matsumoto, Nawawi Ds, Wasrin Shafii, Hirotaka Nakamura, Takuya Akiyama, Tomoya Yokoyama, Gyosuke Meshitsuka
  • Organizer: 2007IUFRO All Division 5 Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2007-10-29
• [Presentation] Absolute Amount of b-O-4 Structure in Wood and Non-wood Cell Walls (2007)
  • Author(s): Hirotaka Nakamura, Deded S. Nawawi, Kenya Kondo, Zhenfu Jin, Takuya Akiyama, Yuji Matsumoto
  • Organizer: 14th International Symposium on Wood, Fiber and Pulping Chemistry (ISWFPC)
  • Place of Presentation: Durban, South Africa
  • Year and Date: 2007-06-27
• [Presentation] How lignin Changes Its Chemical Structure depending on the Mechanical Circumstances (2007)
  • Author(s): Yuji Matsumoto, Deded Sarip Nawawi, Wasrin Syafii, Takuya Akiyama, Gyosuke Meshitsuka
  • Organizer: 14th International Symposium on Wood, Fiber and Pulping Chemistry (ISWFPC)
  • Place of Presentation: Durban, South Africa
  • Year and Date: 2007-06-26
• [Remarks]
  • URL: http://www.woodchemistry.fp.a.u-tokyo.ac.jp/index.html