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蛋白相互作用モジュールの組み換えによる分子進化の再構成の試み

Research Project

Project/Area Number 18659076
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

畑 裕  Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (80313237)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2008: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords蛋白質 / 相互作用
Research Abstract

生命の基本単位である細胞は、蛋白、脂質、糖質、核酸から構成されるが、中でも蛋白同士の相互作用は細胞の構造の大枠を決定する点で重要である。ほとんどの蛋白分子は、蛋白相互作用に関わるモジュールをもち、その組み合わせを利用して、多彩な蛋白相互作用を実現している。本研究では1)人為的な蛋白相互作用モジュールを組み合わせて、実際に自然界に存在するよりも、より強固な蛋白相互作用を実現できるかどうか、2)より強固な蛋白相互作用をもつ蛋白分子を、細胞レベルで淘汰選択することが可能かを、明らかにしようとしている。平成18年度は既知の蛋白相互作用であるWW領域とproline-rich配列をもとに蛋白相互作用の強弱をレポーターによって検出する仕組みの構築を行い、平成19年度には、低分子量GTP結合蛋白質RhoAを用いてRhoAの活性化に応じて蛋白質相互作用に変化を起こすことを試みた。平成20年度には、種々の細胞と種々の刺激を用いて実験を継続した。同時に第三の方法として、細胞内の蛋白相互作用を、分子局在によってモニターする系の構築に着手し、自家発光蛍光タグを融合した複数の蛋白の遺伝子と、発現ライブラリーを同時に細胞に組み込むためのレンチウイルスベクターの作製を行った。

Report

(3 results)
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007 2006 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Threonine 74 of MOBl is a putative key phosphorylation site by MST2 to form the scaffold to activate nuclear Dbf2-related kinase 1.2008

    • Author(s)
      Hirabayashi s., et al.
    • Journal Title

      Oncogene 27

      Pages: 4181-4192

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Co-localization of a novel transcriptional repressor simiRP58 with RP5 8.2008

    • Author(s)
      Takahashi A., et al.
    • Journal Title

      Biochem. Biophys. Res. Comm. 368

      Pages: 637-642

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-SCAMalpha is a scaffold to mediate NMDA receptor-dependent RhoA activation in dendrites2007

    • Author(s)
      Iida J, et. al.
    • Journal Title

      Molecular Cellular Biology 27

      Pages: 4388-4405

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ras-association domain family protein 6 induces apoptosis via both caspase-dependent and caspase-independent pathways.2007

    • Author(s)
      Ikeda M, et. al.
    • Journal Title

      Experimental Research 313

      Pages: 1484-1495

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-SCAM/MAGI-2 is associated with cell adhesion molecules at inhibitory synapses at rat hippocampal neurons.2007

    • Author(s)
      Sumita K, et. al.
    • Journal Title

      Journal of Neurochemistry 100

      Pages: 154-166

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase-Min rat developing and adult lung.2007

    • Author(s)
      Fujiwara N, et. al.
    • Journal Title

      Respirology 12

      Pages: 54-62

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] JAM4, expresses in stem cell- and progenitor cell populations of both male germ cell and hematopoietic cell lineages.2006

    • Author(s)
      Nagamatsu, G., et al.
    • Journal Title

      Mol. Cell. Biol. (Epub ahead of print)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] S-SCAM/MAGI-2 is associated with cell adhesion molecules at inhibitory synapses at rat hippocampal neurons.2006

    • Author(s)
      Sumita, K., et al.
    • Journal Title

      J. Neurochem. (Epub ahead of print)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxyeptidase-M in rat developing and adult lung.2006

    • Author(s)
      Fujiwara, N., et al.
    • Journal Title

      Respirology (Epub ahead of print)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ligand-of-Numb protein X is an endocytic scaffold for junctional adhesion molecule 4.2006

    • Author(s)
      Kansaku, A., et al.
    • Journal Title

      Oncogene 25・37

      Pages: 5071-5084

    • Related Report
      2006 Annual Research Report
  • [Journal Article] CIN85 is localized at synapses and forms a complex with S-SCAM via Dendrin.2006

    • Author(s)
      Kawata, A., et al.
    • Journal Title

      J. Biochem. 139・5

      Pages: 931-939

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Receptor for advanced glycation end-products is a marker of type I cell injury in acute lung injury.2006

    • Author(s)
      Uchida, T., et al.
    • Journal Title

      Am. J. Respir. Crit. Care Med. 173・9

      Pages: 1008-1015

    • Related Report
      2006 Annual Research Report
  • [Presentation] RASSF6とMammalian Sterile 20-like kinase (MST)2の相互作用とその機能の解析2007

    • Author(s)
      川田 現, 他
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] MST2とMOB1によるNuclear Dbf2-related kinase 1の活性化機構とその生理的意義の検討2007

    • Author(s)
      日高 志穂, 他
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] RASSF3による細胞死誘導の解析2007

    • Author(s)
      大掛 真太郎, 他
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/med/mmch/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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