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免疫学的チューター、樹状細胞による新免疫機能検査

Research Project

Project/Area Number 18659163
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionNagasaki University

Principal Investigator

上平 憲  Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (80108290)

Co-Investigator(Kenkyū-buntansha) 山田 恭暉 (山田 恭彰)  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (60145232)
長谷川 寛雄  長崎大学, 大学院・医歯薬学総合研究科, 助教 (00398166)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2008: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsimmunology / dendritic cell / Treg cell / laboratory / phagocyte / laboratory test
Research Abstract

個体の免疫能は種類の異なるいくつもの免疫担当細胞どうしのクロストークで成り立っている。そのような一般的概念の中で、新しい免疫機能検査になり得るものとして、1細胞の動的かつ連続的な解析を行っている。本年度は、昨年に引き続き病原微生物に対する樹状細胞の反応を解析した。
1)感染性微生物である肺炎球菌や緑膿菌を共培養した際の、樹状細胞の運動機能の評価を行った。感染症発生時の樹状細胞の運動パターンを解析した。無刺激の樹状細胞に比べ、細菌との共培養を行った樹状細胞において運動機能が亢進する結果を得た。
2)樹状細胞に影響を与える薬剤のスクリーニングに関する基礎研究を実施した。樹状細胞の細胞培養液中に免疫抑制剤を添加することが、樹状細胞の運動機能を抑制し、細菌貪食率を低下させる結果を得た。
3)生菌と加熱処理した死菌を用いて、樹状細胞の反応性を解析した。加熱処理した死菌に対しては、樹状細胞の運動性が低下していた。
4)健常人数名の解析で、樹状細胞の反応に個人差があることも判明した。
5)感染症を発症している患者の樹状細胞は、病原体に対する運動機能や貪食能の亢進が認められたが、細胞間のバラツキも大きかった。観察および評価する細胞で結果が大きく異なっていた。
One cellでどの程度の個体の免疫能を評価出来るか未だ一定の成果は得られていない。少なくとも自然免疫能の一部は一個の樹状細胞で何らかの免疫の評価に寄与できることは示唆された。

Report

(3 results)
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006

All Journal Article (15 results) (of which Peer Reviewed: 9 results) Presentation (2 results) Book (1 results)

  • [Journal Article] In vivo efficacy and pharmacokinetics of tomopenem (CS-023), a novel carbapenem, against Pseudomonas aeruginosa in a murine chronic respiratory tract infection model.2008

    • Author(s)
      Morinaga Y, Yanagihara K, Kamihira s, et al
    • Journal Title

      J Antimicrob Chemother 62

      Pages: 1326-1331

    • NAID

      120006983741

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of ME1036 against meticillin-resistant Staphylococcus aureus and vancomycin-insensitive S. aureus in a model of haematogenous pul monary infection.2008

    • Author(s)
      Yanagihara K, Ohnishi Y, Kamihira S., et al
    • Journal Title

      Int J Antimicrob Agents. 32

      Pages: 401-404

    • NAID

      120006984639

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subinhibitory concentrations of telithromycin, clarithromycin and azithromycin reduce methicillin-resistant Staphylococcus aureus coagulase in vitro and in vivo.2008

    • Author(s)
      Yanagihara K, Kamihira S, Morinaga Y., et al
    • Journal Title

      J Antimicrob Chemother. 61

      Pages: 647-650

    • NAID

      120006984582

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay.2008

    • Author(s)
      Fukushima KY, Yanagihara K, Kamihira S, et al
    • Journal Title

      J Clin Microbiol 46

      Pages: 2384-2388

    • NAID

      120006983958

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Foxp3 expression on normal and leukemic CD4+CD25+T cells implicated in human T-cell leukemia virus type-l is inconsistent with Treg cells.2008

    • Author(s)
      Abe M, Yanagihara K, Kamihira S, et al
    • Journal Title

      Eur J Haematol 81

      Pages: 209-217

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristic expression of HTLV-l basic zipper factor (HBZ)transcripts in HTLV-l provirus-positive cells.2008

    • Author(s)
      Usui T, Yanagihara K, Kamihira S, et al
    • Journal Title

      Retrovirology ON linejournal

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inactivation of p14ARF as a key event for the progression of adult T cell leukemia/lymphoma.2007

    • Author(s)
      Kohno T, Kamihira.S., et. al.
    • Journal Title

      Leuk Res 31(12)

      Pages: 1625-32

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High IL-21 receptor expression and apoptosis induction by IL-21 in follicular lymphoma.2007

    • Author(s)
      Akamatsu N, Kamihira S., et. al.
    • Journal Title

      Cancer Lett 256(2)

      Pages: 196-206

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) through 15-deoxy-Delta 12, 14 prostaglandin J2 production.2007

    • Author(s)
      Hasegawa H, Kamihira S., et. al.
    • Journal Title

      Blood 110(5)

      Pages: 1664-74

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genomic analysis of ATL2007

    • Author(s)
      Choi YL, Kamihira S., et al.
    • Journal Title

      Oncogene 26(8)

      Pages: 1245-1255

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Survey Of CCR expression reveals frequent co-expression of skin homing CCR4 and CCR10 in ATLL.2006

    • Author(s)
      Harasawa H, Kamihira S, et al.
    • Journal Title

      Leuk Lymphoma 47(10)

      Pages: 2163-2173

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rapid Screening of Topisomerase gene mutations by a novel MCA method for early warning of fluoroquinolone-resistance Strept. Pneu.2006

    • Author(s)
      Fukushima KY, Kamihira S, et al.
    • Journal Title

      J Clin Microbiol 44(2)

      Pages: 4553-4558

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A subtype of aggressive B-cell lymphoma with an usual presentation of effusion without mass2006

    • Author(s)
      Kamihira S., et al.
    • Journal Title

      Leuk Lymphoma 47(9)

      Pages: 1991-1993

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A novel alternative splicing isoform of HTLV-1 bZIP factor targets distinct subnuclear localization2006

    • Author(s)
      Murata K, Kamihira S. et al.
    • Journal Title

      J Virol 80(5)

      Pages: 2495-2505

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparative genomic hybridization analysis of Japanese B-cell cll : Correlation with clinical course2006

    • Author(s)
      Tsukasaki K, Kamihira S, et al.
    • Journal Title

      Leuk Lymphoma 47(2)

      Pages: 261-266

    • Related Report
      2006 Annual Research Report
  • [Presentation] Multiplex real time PCR を用いたH. Influenzaeの迅速診断2008

    • Author(s)
      柳原克紀, 上平 憲
    • Organizer
      臨床自動化学会40回大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] 健康人および HTLV-l関連疾患におけるFoxp3+T-cellの検討2008

    • Author(s)
      安部 壮紀, 上平 憲
    • Organizer
      第9回日本検査血液学会学術集会
    • Place of Presentation
      津
    • Related Report
      2008 Annual Research Report
  • [Book] スタンダード検査血液学2008

    • Author(s)
      上平 憲
    • Publisher
      医歯薬出版
    • Related Report
      2008 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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