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絨毛外トロホブラスト浸潤能へのアクチン・ミオシン発現の関与とその局所・内分泌調節

Research Project

Project/Area Number 18659487
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKobe University

Principal Investigator

丸尾 猛  Kobe University, 大学院・医学系研究科, 教授 (60135811)

Co-Investigator(Kenkyū-buntansha) 松尾 博哉  神戸大学, 医学部, 教授 (60229432)
中林 幸士  神戸大学, 大学院・医学系研究科, 助教 (80362789)
山崎 峰夫  神戸大学, 大学院・医学系研究科, 准教授 (00220301)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords絨毛外トロホブラスト / 浸潤能 / アクチン / ミオシン / 妊娠高血圧症候群 / 胎盤
Research Abstract

アクチンは脊椎動物組織に6種類のアイソフォームが発現し、アイソフォームには3種類のαアクチン(心筋、骨格筋、平滑筋)、1種類のβアクチン(非筋)、2種類のγアクチン(非筋、平滑筋)が含まれ、筋組織や非筋組織の分化の指標となる。胎盤組織ではβ非筋アクチンがα平滑筋アクチンより多く存在する。一方、ミオシンには筋組織や非筋組織中に複数種のミオシン重鎖アイソフォームが存在する。大動脈平滑筋細胞には3〜4種類のMHCアイソフォームがあり、SM1とSM2という平滑筋タイプMHCアイソフォームと、MIIA(NM)という非筋タイプMHCアイソフォームが存在する。
正常末期胎盤に比して妊娠高血圧症候群胎盤では、細胞接着関連分子のfibronectinとその受容体のintegrinα5β1発現は弱く、TIMP-1発現は強い。妊娠高血圧症候群胎盤EVTにおけるアクチンならびにミオシン各種アイソフォーム発現を正常胎盤のそれと比較し、細胞接着関連分子とアクチンならびにミオシンの機能相関として、TIMP-1に焦点を当て検討した。また着床不全の内分泌因子であるプロゲステロンと甲状腺ホルモンのEVTでのアクチンならびにミオシン発現への影響を検討した。さらに、妊娠各期胎盤EVTにおけるアクチンならびにミオシン各種アイソフォームの局在を妊娠極初期(妊娠4-5週)、妊娠初期(妊娠6-12週)、妊娠末期(妊娠37-41週)において、アクチンならびにミオシン各種アイソフォームの局在を特異的モノクロナール抗体を用いて検討した。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (9 results)

All 2008 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (1 results) Book (1 results)

  • [Journal Article] The effects of progesterone on apoptosis in the human trophoblast-derived HTR-8/SV neo cells2008

    • Author(s)
      Liu J, Matsuo H, Laoag-Fernandez JB, Xu Q, Maruo T
    • Journal Title

      Mol Hum Reprod (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concentration-dependent effects of a selective estrogen receptor modulator raloxifene on proliferation and apoptosis in human uterine leiomyoma cells cultured in vitro2007

    • Author(s)
      Liu J, Matsuo H, Xu Q, Chen W. Wang J, Maruo T
    • Journal Title

      Hum Reprod 22

      Pages: 1253-1259

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective progesterone receptor modulator asoprisnil induces endoplasmic reticulum stress in cultured human uterine leiomyoma cells2007

    • Author(s)
      Xu Q, Ohara N, Liu J, Nakabayashi K, DeManno D, Chwalisz K, Yoshida S, Maruo T
    • Journal Title

      Am J Physiol Endocrinol Metab 293

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Matrix metalloproteinase (MMP)-1 and MMP-9 promoter polymorphisms are not associated with an increased risk of uterine leiomyomas in a Japanese population2006

    • Author(s)
      Takemura N, Yoshida S, Kennedy S, Deguchi M, Ohara N, Maruo T
    • Journal Title

      J Soc Gynecol Investig 13

      Pages: 232-236

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Matrix metal loproteinase-1 and -9 promoter polymorphisms and endometrial carcinoma risk in a Japanese population2006

    • Author(s)
      Sugimoto M, Yoshida S, Kennedy S, Deguchi M, Ohara N, Maruo T
    • Journal Title

      J Soc Gynecol Investig 13

      Pages: 523-529

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prevention of postpartum hemorrhage by uterotonic agents : comparison of oxytocin and methylergometrine in the management of the third stage of labor2006

    • Author(s)
      Fujimoto M, Takeuchi K, Sugimoto M, Maruo T
    • Journal Title

      Acta Obstet Gynecol Scand 85

      Pages: 1310-1314

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The CYP17 MspAl polymorphism is not associated with an increased risk of uterine leiomyomas in a Japanese population2006

    • Author(s)
      Tsujino T, Ohara N, Yoshida S, Kennedy S, Takemura N, Deguchi M, Maruo T
    • Journal Title

      Gynecol Endocrinol 22

      Pages: 87-91

    • Related Report
      2006 Annual Research Report
  • [Presentation] Progesterone and progesterone receptor modulator in uterine leiomyomagrowth: their implication in reproductive health2007

    • Author(s)
      Maruo T, Matsuo H, Samoto T, Nakago S, Xu Q, Chen W, Wang J, Ohara N
    • Organizer
      The 12th Annual Conference of Egyptian Fertility and Sterility Society
    • Place of Presentation
      Cairo, Egypt,
    • Related Report
      2007 Annual Research Report
  • [Book] Translational research in uterine biology2008

    • Author(s)
      Ohara N, Xu Q, Matsuo H, Maruo T
    • Publisher
      Elsevier(In press)
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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