• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

グリコサミノグリカン糖鎖リモデリングによる高効率な視神経再生治療の開発

Research Project

Project/Area Number 18659514
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKumamoto University

Principal Investigator

稲谷 大  Kumamoto University, 医学部附属病院, 講師 (40335245)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords酵素 / 神経科学 / 生理活性 / 糖鎖 / 再生医学 / 神経化学 / 整理活性
Research Abstract

ヘパラン硫酸は、さまざまな生理活性物質と結合能を有するグリコサミノグリカンの一つであり、そのヘパラン硫酸を合成する最も重要な酵素の一つであるEXT1を網膜組織特異的に欠損させたコンディショナルノックアウトマウスを作成し、その解析をおこなった。Cre-loxPシステムを用い、Creトランスジェニックマウスには、DKK3 promoter-driven Creマウスを用いて、胎生期の早期からEXT1を網膜組織特異的にノックアウトさせた。そのマウスの網膜では、視神経乳頭の低形成がみられ、視神経の軸索は、網膜を縦断し、網膜視細胞のさらに外側に誤った投射を示していた。Brn3bやTuj1などの網膜神経節細胞のマーカーとなる蛋白の抗体で、その網膜の免疫染色をおこなったところ、網膜神経節細胞の配列は、網膜の内層にとどまっており、また、視細胞のマーカーとなるOtx2に対する抗体で免疫染色をおこなうと、視細胞の配列も網膜外層にとどまっていることが判り、網膜の層構造形成が障害されたために、網膜神経節細胞の軸索投射が乱れたわけではないことが示唆された。したがって、視神経乳頭の形成には、ヘパラン硫酸が必要であることが本研究から判明し、以前に我々は視交叉形成におけるヘパラン硫酸の必要性も報告しており、正常な視神経の投射メカニズムはヘパラン硫酸の発現と密接な関係があることが明らかとなった。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Netrin-1/DCC signaling in commissural axon guidance requires cell autonomous expression of heparan sulfate.2007

    • Author(s)
      Matsumoto Y, Irie F, Inatani M, Tessier-Lavigne M, Yamaguchi Y
    • Journal Title

      Journal of Neuroscience 27

      Pages: 4342-4350

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ocular decompression retinopathy following trabeculectomy with mitomycin C associated with familial amyloidotic polyneuropathy.2006

    • Author(s)
      Wakita M, Kawaji T, Ando E, Koga T, Inatani M, Tanihara H, Ando Y
    • Journal Title

      British Journal of Ophthalmology 90

      Pages: 515-516

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Elevated neprilysin activity in vitreous of patients with proliferative diabetic retinopathy.2006

    • Author(s)
      Hara H, Oh-hashi K, Yoneda S, Shimazawa M, Inatani M, Tanihara H, Kiuchi K
    • Journal Title

      Molecular Vision 19

      Pages: 977-982

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Y-27632, a Rho-associated protein kinase inhibitor, attenuates neuronal cell death after transient retinal ischemia.

    • Author(s)
      Hirata A, Inatani M, Inomata Y, Yonemura N, Kawaji T, Honjo M, Tanihara H
    • Journal Title

      Graefe's Archive for Clinical and Experimental Ophthalmology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraocular pressure-lowering effects and safety of topical administration of a selective ROCK inhibitor, SNJ-1656, in normal volunteers.

    • Author(s)
      Tanihara H, Inatani M, Honjo M, Tokushige H, Azuma J, Araie M.
    • Journal Title

      Archives of Ophthalmology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequency and risk factors for intraocular pressure elevation after posterior sub-Tenon's capsule triamcinolone acetonide injection.

    • Author(s)
      Iwao K, Inatani M, Kawaji T, Koga T, Mawatari Y, Tanihara H
    • Journal Title

      Journal of Glaucoma (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of topical administration of Y-39983, a selective Rho-associated protein kinase inhibitor, on ocular tissues in rabbits and monkeys.

    • Author(s)
      Tokushige H, Inatani M, Nemoto S, Sakaki H, Katayama K, Uehata M, Tanihara H
    • Journal Title

      Investigative ophthalmology & Visual Science (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of pivastatin on experimental choroidal neovascularization in rats.

    • Author(s)
      Sagara N, Kawaji T, Takano A, Inomata Y, Inatani M, Fukushima M, Tanihara H
    • Journal Title

      Experimental Eye Research (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Stability of central visual field after modern trabeculectomy techniques in eyes with advanced glaucoma

    • Author(s)
      Awai M, Koga T, Inatani M, Inoue T, Tanihara H
    • Journal Title

      Japanese Journal of Ophthalmology (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Presentation] Heparan sulfate is required for the development of optic nerve head.2007

    • Author(s)
      Ogata M, Inatani M, Iwao K, Tanihara H.
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/naiyou.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi