Plasmid-mediated augmentation for wastewater treatment

• Project/Area Number: 18681013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Environmental technology/Environmental materials
• Research Institution: Osaka University
• Principal Investigator: SOUDA Satoshi Osaka University, 大学院・工学研究科, 准教授 (30322176)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2006: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 難分解性物質 / 廃水処理 / プラスミド / オーグメンテーション / 排水処理 / 2, 4-D

Research Abstract

2,4-ジクロロフェノキシ酢酸(2,4-Dを部分分解できるプラスミドpJP4を用い、連続回分式活性汚泥プロセス(SBR)にプラスミドオーグメンテーションを実施した。pJP4を導入しなかった対照系SBRは、2,4-Dを分解できなかった。2,4-Dを完全分解できるJMP134 (pJP4)を導入したSBRは、3日目まで高い分解率を示した後、JMP134の淘汰に伴って分解率が低下し、transconjugantが増加した20日目から分解率が回復した。2,4-Dを分解できないHB101 (pJP4)を導入したSBRは、7日間は2,4-Dを分解できなかったが、transconjugantが増加した20日目から分解率が向上した。

Research Products

• [Journal Article] Occurrence and persistence of indigenous transconjugants carrying conjugative plasmids in soil. (2009)
  • Author(s): Inoue, D., Soda, S., Tsutsui, H., Yamazaki, Y., Murashige, K., Sei, K., Fujita, M., and Ike, M.
  • Journal Title: J. Biosci. Bioeng. in press
  • NAID: 110007386288
• [Journal Article] プラスミドオーグメンテーションによる廃水処理プロセスの性能向上の可能性 (2008)
  • Author(s): 惣田訓, 筒井裕文, 井上大介, 清和成, 池道彦
  • Journal Title: 月刊水 50(11) Pages: 14-21
  • NAID: 40016166149
• [Journal Article] Transfer of antibiotic multiresistant plasmid RP4 from Escherichia coli to activated sludge bacteria. (2008)
  • Author(s): Soda, S., Ohtsuki, H., Inoue, D., Tsutsui, H., Sei, K., and Ike, M.
  • Journal Title: J. Biosci. Bioeng. 106(3) Pages: 2929-296
  • NAID: 110006950581
  • Peer Reviewed
• [Journal Article] Transfer of antibiotic multiresistant plasund RP4 from Escherichia colito activated sludge bacteria (2008)
  • Author(s): Soda S, Ohtsuki H, Inoue D, Tsutsui H, Sei. K, Ike M
  • Journal Title: Journal of Bioscience and Bioengineering 106(3) Pages: 292-296
  • Peer Reviewed
• [Journal Article] 伝達性プラスミドの環境内挙動のモデル解析 (2006)
  • Author(s): 惣田訓, 山崎祐二, 村重勝士, 井上大介, 清和成, 池道彦, 藤田正憲
  • Journal Title: 日本水処理生物学会誌別巻 26 Pages: 69-69
  • NAID: 10020294169
• [Presentation] Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to activated sludge-derived bacteria by filter mating. (2009)
  • Author(s): Tsutsui, H., Matsuda, M., Anami, Y., Inoue, D., Sei, K., Soda, S., and Ike, M.
  • Organizer: International Symposium on Frontiers of Environmental and Industrial Biotechnology
  • Place of Presentation: 豊中
  • Year and Date: 2009-01-21
• [Presentation] Transfer of plasmid pJP4 from Eschenchia coli and Pseudomonas putida to activated sludge-derived bacteria by filter mating (2009)
  • Author(s): Tsutsm, H, Matsuda, M, Anami, Y, Inoue, D, Sei, K, Soda, S, and Ike, M
  • Organizer: International Symposium on Frontiers of Environ mental and Industrial Biotechnology
  • Place of Presentation: 大阪千里阪急ホテル
  • Year and Date: 2009-01-21
• [Presentation] フィルターメイティングによる活性汚泥細菌群へのプラスミドpJP4の伝播 (2008)
  • Author(s): 穴見泰崇, 筒井裕文, 松田真佐美, 井上大介, 清和成, 惣田訓, 池道彦
  • Organizer: 日本水処理生物学会第45回大会
  • Place of Presentation: 秋田
  • Year and Date: 2008-11-13
• [Presentation] フィルターメイティングによる活性汚泥細菌群へのプラスミドpJP4の伝播 (2008)
  • Author(s): 穴見泰崇, 筒井裕文, 松田真佐美, 井上大介, 清和成, 惣田訓, 池道彦
  • Organizer: 日本水処理生物学会第45回大会
  • Place of Presentation: 秋田市文化会館
  • Year and Date: 2008-11-13
• [Presentation] Occurrence and persistence of indigenous transconjugants carrying conjugative plasmids in soil microcosms. (2008)
  • Author(s): Inoue, D., Soda, S., Tsutsui, H., Yamazaki,Y., Murashige, K., Sei, K., Fujita, M., and Ike, M.
  • Organizer: International Biotechnology Symposium and Exhibition
  • Place of Presentation: 中国大連
  • Year and Date: 2008-10-14
• [Presentation] Occurrence and persistence of indigenous transconjugants carrying conjugative plasmids in soil microcosms (2008)
  • Author(s): Inoue D, Soda S, Tsutsui H, Yamazaki Y, Murashige K, Sei K, Fupta M, Ike M
  • Organizer: International Biotechnology Symposium and Exhibition(LBS2008)
  • Place of Presentation: 大連, 中国
  • Year and Date: 2008-10-14
• [Presentation] Transfer of plasmid pJP4 from Escherichia coli by filter mating to and its expression in activated sludge-derived bacteria (2008)
  • Author(s): Tsutsui, H., Takeda, S., Otsuki, H., Inoue, D., Sei, K., Soda, S., and Ike, M.
  • Organizer: International Biotechnology Symposium and Exhibition
  • Place of Presentation: 中国大連
  • Year and Date: 2008-10-13
• [Presentation] Transfer of plasmid pJP4 from Escherirchia coli by filter mating to and its expression in activated sludge-derived bacteria (2008)
  • Author(s): Tsutsui H, Takeda S, Otsuki H, Inoue D, Sei K, Soda S, Ike M
  • Organizer: International Biotechnology Symposium am Exhibition(IBS2008)
  • Place of Presentation: 大連, 中国
  • Year and Date: 2008-10-13
• [Presentation] 2,4-D汚染土壌スラリーの外来菌導入による浄化が土着微生物生態系に及ぼす影響の評価 (2007)
  • Author(s): 筒井裕文, 山崎祐二, 井上大介, 清和成, 惣田訓, 池道彦, 藤田正憲
  • Organizer: 第41回日本水環境学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-14
• [Presentation] Transfer of antibiotic multiresistant plasmid RP4 from Escherichia coli to activated sludge bacteria (2007)
  • Author(s): Soda, S., Otsuki, H., Inoue, D., Tsutsui, H., Sei, K., Fujita, M., and Ike, M.
  • Organizer: WaterMicro 2007, 14th International Symposium on health-Related Water Microbiology
  • Place of Presentation: The University of Tokyo
• [Presentation] 伝達性プラスミドの環境内挙動のモデル解析 (2006)
  • Author(s): 惣田訓, 山崎祐二, 村重勝士, 井上大介, 清和成, 池道彦, 藤田正憲
  • Organizer: 日本水処理生物学会第43回大会
  • Place of Presentation: 仙台
  • Year and Date: 2006-11-15