Interaction network analysis of chromosome structural proteins

• Project/Area Number: 18681032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied genomics
• Research Institution: Osaka University
• Principal Investigator: UCHIYAMA Susumu Osaka University, 大学院・工学研究科, 助教 (90335381)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2006: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
• Keywords: 染色体 / タンパク質 / 相互作用解析 / プロテオーム解析 / ネットワーク / 高次構造 / 質量分析 / 分裂期 / ヘテロクロマチン / クロマチン / 複合体 / コンデンシ / コンデンシン

Research Abstract

報告者らが行ったヒト染色体プロテオーム解析において染色体高次構造への関与が推定されたBP74をターゲットの一つとして研究を進め、免疫沈降法などにより相互作用タンパク質を網羅的に同定した。また、BP74と相互作用することが分かったHP1との相互作用パラメータを物理化学的手法により決定した。さらに、BP74の立体構造決定と組換ヒト染色体タンパク質を用いた実験から、BP74 がクロマチン高次構造形成へと繋がるクロマトソーム構造形成を担っていることが判明した。本研究により染色体プロテオーム解析で同定された染色体を中心とする相互作用ネットワークを明らかとすることに成功した。

Research Products

• [Journal Article] A nucleolar protein RRS1 contributes to chromosome congression. (2009)
  • Author(s): Gambe, A.E., Matsunaga, S., Takata, H., Ono-Maniwa, R., Baba, A., Uchiyama, S. and Fukui, K.
  • Journal Title: FEBS Letters in press
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragment containing Aegilops tauschii genes. (2009)
  • Author(s): Wada N., Kajiyama S., AkiyamaY., Kawakami S., No D., Uchiyama S., Otani M., Shimada T., Nose N., Suzuki G., Mukai Y. and Fukui K.
  • Journal Title: Plant Cell Reports in press
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragment containing Aegilops tauschii genes (2009)
  • Author(s): Wada N., Kajiyama S., Akiyama Y., Kawakami S., No D., Uchiyama S., Otani M., Sshimada T., Nose N., Suzuki G., Mukai Y. and Fukui K.
  • Journal Title: Plant Cell Reports (In press)
  • Peer Reviewed
• [Journal Article] Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells. (2008)
  • Author(s): Amin MA, Matsunaga S, Uchiyama S, Fukui K.
  • Journal Title: FEBS Lett. 582 Pages: 3839-44
• [Journal Article] Isolation method for human metaphase chromosomes. (2008)
  • Author(s): Hayashihara, K., Uchiyama, S., Kobayashi, S., Yanagisawa, M., Matsunaga, S., Fukui K.
  • Journal Title: Nature Protocol Network nprot Pages: 166-166
• [Journal Article] Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells. (2008)
  • Author(s): Amin MA, Matsunaga S, Uchiyama S, Fukui K.
  • Journal Title: Biochem J. 415 Pages: 345-351
  • NAID: 120006995003
• [Journal Article] Live cell imaging reveals plant Aurora kinase has dual roles during mitosis. (2008)
  • Author(s): Kurihara, D., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 256-1261
  • NAID: 10027333174
• [Journal Article] Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis. (2008)
  • Author(s): Fukui, K., Takata, H., and Uchiyama, S.
  • Journal Title: Methods Mol. Biol. 432 Pages: 149-160
• [Journal Article] The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen. (2008)
  • Author(s): Cartagena, J.A., Matsunaga, S., Seki, M., Kurihara, D., Yokoyama, M., Shinozaki, K., Fujimoto, S., Azumi, Y., Uchiyama, S., Fukui, K.
  • Journal Title: Dev. Biol. 315 Pages: 355-368
• [Journal Article] Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis (2008)
  • Author(s): Fukui, K., Takata, H., and Uchiyama, S.
  • Journal Title: Methods Mol. Biol. 432 Pages: 149-160
  • Peer Reviewed
• [Journal Article] Live cell imaging reveals plant Aurora kinase has dualroles during mitosis (2008)
  • Author(s): Kurihara, D., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1256-1261
  • Peer Reviewed
• [Journal Article] Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells (2008)
  • Author(s): Amin MA, Matsunaga S, Uchiyama S, Fukui K
  • Journal Title: Biochem J. 415 Pages: 345-351
  • NAID: 120006995003
  • Peer Reviewed
• [Journal Article] Isolation method for human metaphase chromosomes. 166 (2008)
  • Author(s): Hayashihara, K., Uchiyama, S., Kobayashi, S., Yanagisawa, M., Matsunaga, S., Fukui K.
  • Journal Title: Nature Protocol Network nprot. 2008 Pages: 166-166
• [Journal Article] Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells (2008)
  • Author(s): Amin MA, Matsunaga S, Uchiyama S, Fukui K
  • Journal Title: FEBS Lett. 582 Pages: 3839-3844
  • Peer Reviewed
• [Journal Article] The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen. (2008)
  • Author(s): Cartagena, J, A., Matsunaga, S., Seki, M., Kurihara, D., Yokoyama, M., Shinozaki, K., Fujimoto, S., Azumi, Y., Uchiyanma. S., Fukui, K.,
  • Journal Title: Dev. Biol. In press
  • Peer Reviewed
• [Journal Article] Thermal unfolding mechanism of lipocalin-type prostaglandin D synthase. (2008)
  • Author(s): Iida, T., Nishimura, S., Mochizuki, M, Uchiyama, S., Ohkubo, T., Urade, U., Tanaka, A., and Inui, T.
  • Journal Title: FEBS J. 275 Pages: 233-241
  • Peer Reviewed
• [Journal Article] Anti-peptide antibodies for examining the conformation, molecular assembly, and localization of an intracellular protein, ribosormal protein S6, in vivo. (2008)
  • Author(s): Nakagawa, M., Ohmido, N., Ishikawa, K., Uchiyama, S., ukui, K., and Azuma, T.
  • Journal Title: J. Biochemistry 143 Pages: 325-332
  • Peer Reviewed
• [Journal Article] Anti-peptide antibodies for examining the conformation, molecular assembly, and localization of an intracellular protein, ribosormal protein S6, in vivo. (2007)
  • Author(s): Nakagawa, M., Ohmido, N., Ishikawa, K., Uchiyama, S., Fukui, K., and Azuma, T.
  • Journal Title: J. Biochemistry 143 Pages: 325-332
• [Journal Article] PHB2 protects sister chromatid cohesion in mitosis. (2007)
  • Author(s): Takata, H., Matsunaga, S., Morimoto, A., Ma, N., Kurihara, D., Maniwa-Ono, R., Nakagawa, M., Azuma, T., Uchiyama, S., Fukui, K.
  • Journal Title: Current Biology 17 Pages: 1356-61
• [Journal Article] H1.X with different properties from other linker histones is required for mitotic progression. (2007)
  • Author(s): Takata, H., Matsunaga, S., Morimoto, A., Maniwa-Ono, R., Uchiyama, S., Fukui, K.
  • Journal Title: FEBS Lett. 581 Pages: 3783-8
• [Journal Article] Fibrillarin, a nucleolar protein is required for normal nuclear morphology and cellular growth in HeLa cells. (2007)
  • Author(s): Amin, M.A. Matsunaga, S., Ma, N., Takata, H., Yokoyama, M., Uchiyama, S., Fukui, K.
  • Journal Title: Biochem. Biophys. Res. Comm. 360 Pages: 320-6
• [Journal Article] Histone H2A mobility is regulated by its tails and acetylation ofcore histone tails. (2007)
  • Author(s): Higashi, T., Matsunaga, S., Isobe, K., Morimoto, A., Shimada, T., Kataoka, S., Watanabe, W., Uchiyama, S., Itoh, K., and Fukui. K
  • Journal Title: Biochem. Biophys. Res. Comm. 357 Pages: 627-632
• [Journal Article] Chromosome protein framework from proteome analysis of isolated human metaphase chromosomes. (2007)
  • Author(s): Fukui, K. and Uchiyama, S.
  • Journal Title: Chemical Rec. 7 Pages: 230-7
• [Journal Article] Nucleolin functions in nucleolus formation and chromosome congression. (2007)
  • Author(s): Ma, N., Matsunaga, S., Takata, H., Ono-Maniwa, R., Uchiyama, S. and Fukui, K.
  • Journal Title: J. Cell Sci. 120 Pages: 2091-2105
  • NAID: 120006995004
• [Journal Article] Characterization of a splicing variant of plant Aurora kinase. (2007)
  • Author(s): Kurihara, D., Kawabe, A., Matsunaga, S., Nakagawa, K., Fujimoto, S., Uchiyama, S. and Fukui, K.
  • Journal Title: Plant Cell Physiol. 48 Pages: 369-374
  • NAID: 10018519891
• [Journal Article] Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics. (2007)
  • Author(s): Gambe, A.G., Maniwa,R., Matsunaga, S., Kutsuna, N., Higaki, T., Higashi, T., Hasezawa, S., Uchiyama, S. and Fukui, K.
  • Journal Title: Cytometry 71 Pages: 286-296
• [Journal Article] A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins. (2007)
  • Author(s): Takata, H., Uchiyama, S., Nakamura, N., Nakashima, S., Kobayashi, S., Sone, T., Kimura S., Lahmers, S., Granzier, H., Labeit, S., Matsunaga, S., and Fukui, K.
  • Journal Title: Genes to Cells 12 Pages: 269-284
• [Journal Article] Crystal Structure of Pyrococcus horikoshii PPC Protein at 1.60 Å Resolution. (2007)
  • Author(s): Lin, L., Nakano, H., Nakamura, S., Uchiyama, S., Fujimoto, S., Matsunaga, S., Kobayashi, Y., Ohkubo, T., and Fukui, K.
  • Journal Title: Proteins: Structure, Function and Bioinfomatics 67 Pages: 505-507
• [Journal Article] Whole-Mount Immuno-Electron Tomography (IET) of Chromosomes and Cells. (2007)
  • Author(s): Engelhardt, P., Merilainen1, J., Zhao, F., Uchiyama, S., Fukui, K., and Lehto, V.-P.
  • Journal Title: Methods in Molecular Biology 369 Pages: 387-405
• [Journal Article] Calcite-specific coupling protein in bamacle underwater cement. (2007)
  • Author(s): Mori, Y., Urushida, Y., Nakano, M., Uchiyama, S., Kamino, K.
  • Journal Title: FEBS J. 274 Pages: 6436-6446
  • Peer Reviewed
• [Journal Article] PHB2 protects sister chromatid cohesion in mitosis. (2007)
  • Author(s): Takata, H., Matsunaga, S., Morimoto, A., Ma, N., Kurihara, D., Maniwa-Ono, R., Nakagawa, M., Azuma, T., Uchiyama, S., Fukui, K
  • Journal Title: Current Biology 17 Pages: 1356-61
  • Peer Reviewed
• [Journal Article] Roles of short connecting desulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c5 (2007)
  • Author(s): Ogawa, K., Sonoyama, T., Takeda, T., lchiki, S., Nakamura, S., Kobayashi, Y., Uchiyama, S., Nakasone, K., takayama, S. J., Mita, H., yamamoto, Y., and Sambongi, Y.
  • Journal Title: Extremophiles 11 Pages: 797-807
  • Peer Reviewed
• [Journal Article] Fibrillarin, a nucleolar protein is required for normal nuclear morphology and cellular growth in HeLa cells. (2007)
  • Author(s): Amin, M.A. Matsunaga, S., Ma, N., takata, H., Yokoyama, M., Uchiyama, S., Fukui, K.
  • Journal Title: Biochem. Biophys. Res, Comm 360 Pages: 320-6
  • Peer Reviewed
• [Journal Article] Histone H2A Mobility is regulated by its tails and acetylation ofcore histone tails (2007)
  • Author(s): Higashi, T., Matsunaga, S., Isobe, K., Morimoto, A., Shimada, T., Kataoka, S., Watanabe, W., Uchiyama, S., Itoh, K., and Fukui. K
  • Journal Title: Biochem. Biophys. Res. Comm. 357 Pages: 627-632
  • Peer Reviewed
• [Journal Article] Chromosome protein framework from proteome analysis of isolated human metaphase chromosomes. (2007)
  • Author(s): Fukui, K. and Uchiyama. S.
  • Journal Title: Chemical Rec. 7 Pages: 230-237
  • Peer Reviewed
• [Journal Article] Nucleolin functions in nucleolus formation and chromosome congression (2007)
  • Author(s): Ma, N., Matsunaga, S., Takata, H., Ono-Maniwa, R., Uchiyama, S. and Fukui, K.
  • Journal Title: J. Cell Sci. 120 Pages: 2091-2105
  • NAID: 120006995004
  • Peer Reviewed
• [Journal Article] コラーゲンの三本鎖構造の熱安定性に及ぼす水和の効果 (2007)
  • Author(s): 西 義則、山内 進、小林祐次
  • Journal Title: 熱測定 34 Pages: 152-158
  • Peer Reviewed
• [Journal Article] Characterization of a splicing variant of plant Aurora kinase. (2007)
  • Author(s): Kurihara, D., Kawabe, A., Matsunaga, S., Nakagawa, K., Fujimoto, S., Uchiyama, S., Fukui, K.
  • Journal Title: Plant Cell Physiol. 48 Pages: 369-374
  • NAID: 10018519891
• [Journal Article] Aurora kinase is required for chromosome segregation in tobacco BY-2 cells. (2006)
  • Author(s): Kurihara, D., Matsunaga, S., Kawabe, A., Fujimoto, S., Noda, M., Uchiyama, S., and Fukui, K.
  • Journal Title: Plant journal 48 Pages: 572-580
• [Journal Article] Calreticulin as a new histone binding protein in mitotic chromosomes. (2006)
  • Author(s): Kobayashi, S., Uchiyama, S., Sone, T., Noda, M., Lin, L., Mizuno, H., Matsunaga, S., and Fukui, K.
  • Journal Title: Cytogenet. Genome Res 115 Pages: 10-15
• [Journal Article] Aurora kinase is required for chromosome segregation in tobacco BY-2 cells. (2006)
  • Author(s): Kurihara, D., Matsunaga, S., Kawabe, A., Fujimoto, S., Noda, M., Uchiyama, S., Fukui, K.
  • Journal Title: Plant journal 48 Pages: 572-580
• [Journal Article] Calreticulin as a new histone binding protein in mitotic chromosomes. (2006)
  • Author(s): Kobayashi, S., Uchiyama, S., Sone, T., Noda, M., Lin, L., Mizuno, H., Matsunaga, S., Fukui, K.
  • Journal Title: Cytogenet. Genome Res 115 Pages: 10-15
• [Journal Article] Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics.
  • Author(s): Gambe, A.G., Maniwa, R., Matsunaga, S., Kutsuna, N., Higaki, T., Higashi, T., Hasezawa, S., Uchiyama, S., Fukui, K.
  • Journal Title: Cytometry (in press)
• [Journal Article] A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins.
  • Author(s): Takata, H., Uchiyama, S., Nakamura, N., Nakashima, S., Kobayashi, S., Sone, T., Kimura S., Lahmers, S., Granzier, H., Labeit, S., Matsunaga, S., Fukui, K.
  • Journal Title: Genes to Cells (in press)
• [Journal Article] Crystal Structure of Pyrococcus horikoshii PPC Protein at 1.60 A Resolution.
  • Author(s): Lin, L., Nakano, H., Nakamura, S., Uchiyama, S., Fujimoto, S., Matsunaga, S., Kobayashi, Y., Ohkubo, T., Fukui, K.
  • Journal Title: Proteins : Structure, Function and Bioinfomatics (in press)
• [Presentation] Protein Composition of human metaphase chromosomes (2008)
  • Author(s): 内山進
  • Organizer: 染色体学会年会、学会賞記念講演
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-03
• [Presentation] Characterization of the novel HP1-binding protein (2008)
  • Author(s): Kayoko HAYASHIHARA, Hidekazu WAKAMATSU, Daisuke NO, Hiroki SUGAHARA, Shouhei KOBAYASHI, Susumu UCHIYAMA, Tadayasu OHKUBO, Sachihiro MATSUNAGA and Kiichi FUKUI
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-03
• [Presentation] Protein composition of human metaphase chromosomes(染色体学会賞受賞) (2008)
  • Author(s): Uchiyama, S
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-02
• [Presentation] Molecular mechanism for morphogenesis of X-shaped chromosomes (2008)
  • Author(s): Sachihiro MATSUNAGA, Hideaki TAKATA, Akihiro MORIMOTO, Daisuke KURIHARA, Susumu UCHIYAMA and Kiichi FUKUI
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-01
• [Presentation] H1. X with different properties from other linker histones has important roles in proper mitotic progression (2008)
  • Author(s): Akihiro Morimoto, Hideaki Takata, Rika Ono-Maniwa, Susumu Uchiyama, Sachihiro Matsunaga and Kiichi Fukui
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-01
• [Presentation] Functional analysis of RBMX in kinetochore formation by combination with RNAi method and electron microscopy (2008)
  • Author(s): Mei Hann LEE, Susumu UCHIYAMA, Sumire INAGA, Sachihiro MAT SUNAGAI, Hirotaro MORI and Kiichi FUKUI
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-01
• [Presentation] Functional analysis of plant Aurora kinase during mitosis (2008)
  • Author(s): Daisuke KURIHARA, Susumu UCHIYAMA, Sachihiro MAT SUNAGA and Kiichi FUKUI
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-01
• [Presentation] Three-dimensional higher-order structure and scaffold of mammalian chromosomes revealed by electron microscopy (2008)
  • Author(s): Sumire INAGA, Tetsuo KAT SUMOTO, Hironobu NAKANE, Tomonori NAGURO, Keiichi TANAKA, Akihiro IINO, Linyen LIN, Susumu Uchiyama, Sachihiro MATSUNAGA and Kiichi FUKUI
  • Organizer: Asia Chromosome Colloquium
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-12-01
• [Presentation] ヒト染色体のプロテオーム解析 (2008)
  • Author(s): 内山進
  • Organizer: 蛋白質科学会、若手奨励賞講演
  • Year and Date: 2008-06-11
• [Presentation] ヒト染色体のプロテオーム解析(若手奨励賞受賞) (2008)
  • Author(s): 内山進
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 船堀タワーホール
  • Year and Date: 2008-06-11
• [Presentation] 分裂中期ヒト染色体のプロテオーム解析 (2007)
  • Author(s): 内山 進、林原加代子、小林昇平、高田英昭、松永幸大、福井希一
  • Organizer: 分子生物学会、生化学会合同年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 分裂中期ヒト染色体のプロテオーム解析 (2007)
  • Author(s): 山内進、林原加代子、高田英明、小林昇平、松永幸大、福井希一
  • Organizer: 分子生物学会、生化学会台同年会
  • Place of Presentation: バシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 構成タンパク質から見たヒト染色体 (2007)
  • Author(s): 内山進、林原加代子、高田英明、小林昇平、松永幸大、福井希一
  • Organizer: 染色体学会
  • Place of Presentation: 総研大(葉山)
  • Year and Date: 2007-11-27
• [Presentation] 構成タンパク質から見たヒト染色体 (2007)
  • Author(s): 山内進、林原加代子、高田英明、小林昇平、松永幸大、福井希一
  • Organizer: 染色体学会
  • Place of Presentation: 総研大、葉山
  • Year and Date: 2007-11-27
• [Presentation] Proteome analyses of human metaphase chromosomes (2007)
  • Author(s): Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Sachihiro Matsunaga, and Kiichi Fukui
  • Organizer: International Chromosome Conference
  • Place of Presentation: RAI, Amsterdam
  • Year and Date: 2007-08-28
• [Presentation] Porteome analyses of human metaphase chromosomes (2007)
  • Author(s): Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Sachihiro Matsunaga, and Kiichi Fukui.
  • Organizer: International Chromosome Conference
  • Place of Presentation: RAL, Amsterdam
  • Year and Date: 2007-08-28
• [Presentation] ヒト染色体のプロテオーム解析 (2006)
  • Author(s): 内山進、小林昇平、高田英昭、石原武、林原加代子、曽根岳史、松永幸大、福井希一
  • Organizer: 染色体学会 2006 年度(第57 回)年会
  • Place of Presentation: 千葉大学西千葉キャンパスけやき会館
  • Year and Date: 2006-11-24
• [Presentation] 染色体の塩処理による新規染色体蛋白質の同定および局在解析 (2006)
  • Author(s): 林原加代子、若松秀和、農大輔、小林昇平、内山進、松永幸大、福井希一
  • Organizer: 染色体学会2006年度(第57 回)年会
  • Place of Presentation: 千葉大学西千葉キャンパスけやき会館
  • Year and Date: 2006-11-24
• [Presentation] ヒト組替えタンパク質を用いたクロマチン再構築 (2006)
  • Author(s): 野田勝紀、島原秀登、楯真一、岩崎憲治、鷹岡昭夫、内山進、松永幸大、福井希一
  • Organizer: 染色体学会2006年度(第57回)年会
  • Place of Presentation: 千葉大学西千葉キャンパスけやき会館
  • Year and Date: 2006-11-24
• [Presentation] 染色体関連タンパク質、特にRRSIのRNAiによる機能解析 (2006)
  • Author(s): 福井希一、松永幸大、内山進、真庭理香、馬場朋子
  • Organizer: 日本遺伝学会第78回大会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2006-09-27
• [Presentation] GEPを用いた染色体タンパク質の局在およびFRAP (2006)
  • Author(s): 森本晃弘、東恒仁、真庭理香、内山進、渡辺歴、松永幸大、伊東一良、福井希一
  • Organizer: 第58回日本生物工学会大会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2006-09-12
• [Presentation] ANi法を用いた分裂期におけるトポイソメラーゼIの機能分析 (2006)
  • Author(s): 山川真理子、高田英昭、内山進、松永幸大、福井希一
  • Organizer: 第58 回 日本生物工学会大会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2006-09-12
• [Presentation] 塩処理法を用いたヒト染色体構造維持に関する染色体タンパク質の同定 (2006)
  • Author(s): 若松秀和、農大輔、林原加代子、藤本聡、内山進、松永幸大、福井希一
  • Organizer: 第58回日本生物工学会大会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2006-09-11
• [Presentation] Proteome analysis of human metaphase chromosomes (2006)
  • Author(s): Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Tsunehito Higashi, Kayoko Hayashihara, Shoko Nakashima, Takefumi Sone, Sachihiro Matsunaga, Fukui Kiichi
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Interbational Conference Hall
  • Year and Date: 2006-06-23
• [Presentation] Interaction analysis of human condensin complex in interphase (2006)
  • Author(s): Shoko Nakashima, Shuichi Miyakawa, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Interbational Conference Hall
  • Year and Date: 2006-06-23
• [Presentation] Comparative proteome analysis of human metaphase chromosomes (2006)
  • Author(s): Hideaki Takata, Naoko Nakamura, Shouhei Kobayashi, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Interbational Conference Hall
  • Year and Date: 2006-06-23
• [Presentation] Morphological Changes in Human Chromosomes by Divalent Cations (2006)
  • Author(s): Linyen Lin, Sumire Inaga, Tetsuo Katsumoto, Tomonori Naguro, Susumu Uchiyama, Sachihiro Matsunaga, Akio Takaoka, Kiichi Fukui
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Interbational Conference Hall
  • Year and Date: 2006-06-23
• [Presentation] Necleolin functions in formation of nucleoli and chromosome congression (2006)
  • Author(s): Nan Ma, Hideaki Takata, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Interbational Conference Hall
  • Year and Date: 2006-06-23
• [Presentation] 二価陽イオンマグネシウム濃度による染色体蛋白質局在の変化及び染色体高次構造の解析 (2006)
  • Author(s): 林麟晏、内山進、松永幸大、稲賀すみれ、勝本哲央、名黒知徳、 鷹岡昭夫、福井希一
  • Organizer: 医学生物学電子顕微鏡技術学会第22回学術講演会および総会
  • Place of Presentation: クリエート浜松
  • Year and Date: 2006-05-13
• [Presentation] 細胞周期を通じたβ-actin 4の局在と染色体分配に対する機能 (2006)
  • Author(s): 福井希一、松永幸大、内山進、真庭理香、中村直子
  • Organizer: 第6回タンパク質科学会年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2006-04-25
• [Presentation] 比較プロテオームによるヒト分裂期染色体タンパク質のプロテオーム解析 (2006)
  • Author(s): 高田英昭、中村直子、小林昇平、木村澄子、内山進、松永幸大、福井希一
  • Organizer: 第6回タンパク質科学会年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2006-04-24
• [Book] Proteome analysis human metaphase chromosomes. Chromosome Nanoscience and Technology (2007)
  • Author(s): Fukui, K. and Uchiyama, S.
  • Publisher: CRC Press.
• [Book] Isolation of human and plant chromosomes as nano-materials. Chromosome Nanoscience and Technology (2007)
  • Author(s): Uchiyama, S., Tomoyuki, D. and Fukui, K.
  • Publisher: CRC Press.
• [Book] On-Chip Chromosom Sorter Using Electric and Magnetic Fields. Chromosome Nanoscience and Technology (2007)
  • Author(s): Inoue, T., Fujita, Y., Uchiyama, S., Doi, T., Fukui, K and Yokoyama, H.
  • Publisher: CRC Press.
• [Book] Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein. Chromosome Nanoscience and Technology (2007)
  • Author(s): Nakagawa, M., Ohmido, N., Ishikawa, K., Uchiyama, S., Fukui, K., and Azuma, T.
  • Publisher: CRC Press.
• [Book] Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATPDependent Chromatin- Assembly Factor. Chromosome Nanoscience and Technology (2007)
  • Author(s): Ohkubo, T., Petkova-Andonova, M., Kojima, Y., Nakamura, S., Fujita, H., Nishi, Y., Nakano, H., Uchiyama, S., Fukui, K., and Kobayashi, Y.
  • Publisher: CRC Press.
• [Book] Dynamic and Functional Analysis of Chromosomal Proteins. Chromosome Nanoscience and Technology (2007)
  • Author(s): Ohmido, N., Uchiyama, S., Matsunaga, S., Nakagawa, M., Azuma, T., and Fukui, T.
  • Publisher: CRC Press.
• [Book] proteome analysis human metaphase chromosomes (2007)
  • Author(s): Fukui, K. and Uchiyama, S.
  • Total Pages: 22
  • Publisher: CRC Prss
• [Book] Isolation of human and plant chromosomes as nano-materials. (2007)
  • Author(s): Uchiyama, S., Tomoyuki, D. and Fukui, K
  • Total Pages: 12
  • Publisher: CRC Press
• [Book] On-Chip Chromosom Sorter Using Electric and Magnetic Fields (2007)
  • Author(s): Inoue, T., Fujita, Y., Uchiyama, S., Doi, T., Fukui, K and Yokoyama, H
  • Total Pages: 10
  • Publisher: CRC Press
• [Book] Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein (2007)
  • Author(s): Nakagawa, M., Ohmido, N., Ishikawa, K., Uchiyama, S., Fukui, K., and Azuma, T.
  • Total Pages: 14
  • Publisher: CRC Press
• [Book] Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATPDependent Chromation-Assembly Factor (2007)
  • Author(s): Ohkubo, T., Petkova-Andonova, M., Kojima, Y., Nakamura, S., Fjita, H., Nishi, Y., Nakano, H., Uchiyama, S., Fukui, K., and Kobayashi, Y.
  • Total Pages: 14
  • Publisher: CRC Press
• [Book] Dynamic and Functional Analysis of Chromosomal Proteins (2007)
  • Author(s): Ohmido, N., Uchiyama. S., Matsunaga, S., Nakagawa, M., azuma, T., and Fukui, T.
  • Total Pages: 14
  • Publisher: CRC Press
• [Book] 植物染色体の単離法 (2006)
  • Author(s): 内山進
  • Publisher: クロモソーム 養賢堂
• [Book] 植物染色体のソーティング法 (2006)
  • Author(s): 内山進
  • Publisher: クロモソーム養賢堂
• [Book] Methods in Molecular Biology
  • Author(s): Engelhardt, P., Merilainenl, J., Zhao, F., Uchiyama, S., Fukui, K., Lehto, V-P.
  • Publisher: Humana Press (In press)
• [Book] Chromosome Nanotechnology
  • Author(s): Uchiyama, S., Doi, T., Fukui, K.
  • Publisher: Taylor ＆Francis(In prers)
• [Remarks] ホームページ
  • URL: http://www.bio.eng.osaka-u.ac.jp/cl/
• [Remarks] 2008 年 染色体学会賞受賞
• [Remarks] 2008 年 蛋白質科学会若手奨励賞受賞