Molecular Mechanism of Photoprotective Function of a Carotenoid Bound to the Photoreaction Center Revealed by Time-Resolved X-Ray Crystallography
Project/Area Number |
18684016
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Condensed matter physics I
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Research Institution | Osaka City University |
Principal Investigator |
FUJII Ritsuko Osaka City University, 大学院・理学研究科, 博士研究員 (80351740)
|
Project Period (FY) |
2006 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000)
Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2007: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2006: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
Keywords | 光物性 / 光合成 / たんぱく質構造 / 物性 / 光誘起協力現象 / エネルギー散逸 / たんぱく質構造・物性 / 蛋白質構造・物性 |
Research Abstract |
紅色光合成細菌Rhodobacter sphaeroides 2.4.1由来の光反応中心複合体の1.95Åの高分解能X線結晶構造解析に日本で初めて成功した。その結果、光合成光保護メカニズムに関与すると考えられるカロテノイドの結合長の変位を検出するためには2.5Å以上の高分解能が必須であることがわかった。また分解能を左右するクライオプロテクションなどの操作を改良することにより、従来よりも小さい光を透過する結晶でも高分解能を得ることに成功した。
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Report
(4 results)
Research Products
(87 results)
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[Journal Article] Characterization by Stark Spectroscopy of Reconstituted Light-Harvesting 1 Complex of Purple Photosynthetic Bacterium Rhodospirillum rubrum with the Polypeptides, Bacteriochlorophyll a, and Carotenoids2008
Author(s)
K. Nakagawa, A. Mizuno, S.Suzuki, R. Fujii, T. Dewa, A.T. Gardiner, R.J. Cogdell, M. Nango, H. Hashimoto
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Journal Title
Carotenoid Science 13
Pages: 38-45
Related Report
Peer Reviewed
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[Journal Article] Coherent spectroscopy of carotenoid and bacteriochlorophyll2008
Author(s)
M. Sugisaki, M. Fujiwara, K. Yanagi, R. Fujii, R. J. Cogdell, and H. Hashimoto
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Journal Title
Photosynthesis.Energy of Sun: 14th International Congress on Photosynthesis Research 2007 (ed.J.F.Allen, et. al., Springer-Verlag, Berlin, Heidelberg, New York, Tokyo)
Pages: 259-262
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Peer Reviewed
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[Journal Article] Structures and Functions of Carotenoids Bound to Reaction Centres from Purple Photosynthetic Bacteria2006
Author(s)
H. Hashimoto, R. Fujii, K. Yanagi, T. Kusumoto, A. T. Gardiner, A. W. Roszak, N. W. Issacs, Z. Pendon, D. Niedzwiedski, H. A. Frank, R. J. Cogdell
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Journal Title
Pure Appl. Chem 78(8)
Pages: 1505-1518
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Peer Reviewed
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[Journal Article] Structures and functions of carotenoids bound to reaction centres from purple photosynthetic bacteria2006
Author(s)
H.Hashimoto, R.Fujii, K.Yanagi, T.Kusumoto, A.T.Gardiner, A.W.Roszak, N.W.Isaacs, Z.Pendon, D.Niedzwiedski, H.A.Frank, R.J.Cogdell
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Journal Title
Pure and Applied Chemistry 78(8)
Pages: 1505-1518
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[Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viridis2008
Author(s)
A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
Organizer
The 15th International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan,
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[Presentation] Probing the Effect of the Binding Site on the Electrostatic Behavior of A Series of Carotenoids Reconstituted into the Light-Harvesting 1 Complex from Purple P hotosynthetic Bacterium Rhodospirillum rubrum Detected by Stark Spectroscopy2008
Author(s)
K. Nakagawa, N. Fukui, T. Nakano, T. Horibe, S. Suzuki, R. Fujii, A.T. Gardiner, R.J. Cogdell, H. Hashimo to and M. Nango
Organizer
15^<th> International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan
Related Report
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[Presentation] Reconstitution of Antenna Light-Harvesting 1 Complexes from the Purple Photosynthetic Bacterium Rhodobacter sphaeroides with LH1-Polypeptides, Bacteriochlorophyll a, and Carotenoids : Carotenoid Specificity2008
Author(s)
N. Fukui, A. Mizuno, T. Nakano, K. Nakagawa, A.T. Gardiner, R. J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
Organizer
15^<th> International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan
Related Report
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[Presentation] Reconstitution of Light-Harvesting 1 Complexes from the Purple Photosynthetic Bacterium Rhodospirillum rubrum with Separately Isolated Polypeptides (LH1-a and LHI-β), Bacteriochlorophyll a, and Carotenoid2008
Author(s)
T. Nakano, N. Fukui, K. Nakagawa, A.T.Gardiner, R.J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
Organizer
15^<th> International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan
Related Report
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[Presentation] Electroabsorption Spectroscopy of Native and Reconstituted LH1 Complexes from Purple Photosynthetic Bacterium Rhodospirillum rubrum2008
Author(s)
T. Horibe, K. Nakagawa, T. Kusumoto, S. Suzuki, R. Fujii, A. T. Gardiner, R.J. Cogdell, M. Nango, H. Hashimoto
Organizer
15^<th> International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan
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[Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viri dis, The 15th International Symposium on Carotenoids2008
Author(s)
A.W. Roszak, A.T. Gardiner, V. Moulisova, A.D. Putranto, R. Fujii, H. Hashimoto, N.W. Isaacs, R.J. Cogdell
Organizer
15^<th> International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan
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[Presentation] Vibronic Coherence of Photosynthetic Pigments2007
Author(s)
M. Sugisaki, M. Fujiwara, R. Fujii, K. Nakagawa, M. Nango, R. J. Cogdell, and H. Hashimoto
Organizer
The 15t International Conference on Luminescence and Optical Spectroscopy of Condensed Matter (ICL'08)
Place of Presentation
Lyon, France
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[Book] In : B. Grimm, R. Porra, W. Rudiger and H. Scheer (eds.), Chlorophylls and Bacteriochlorophylls : Biochemistry, Biophysics, Functions and Applications (Advances in Photosynthesis and Respiration, Vol. 25)2006
Author(s)
Y. Koyama, Y. Kakitani, L. Limantara and R. Fujii, Springer
Publisher
Effects of Axial Coordination, Electronic Excitation and Oxidation on Bond Orders in the Bacteriochlorin Macrocycle, and Generation of Radical Cation on Photo-Excitation of in vitro and in vivo Bacteriochlorophyll a Aggregates : Resonance Raman Study
Related Report