Molecular Mechanism of Photoprotective Function of a Carotenoid Bound to the Photoreaction Center Revealed by Time-Resolved X-Ray Crystallography

• Project/Area Number: 18684016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Condensed matter physics I
• Research Institution: Osaka City University
• Principal Investigator: FUJII Ritsuko Osaka City University, 大学院・理学研究科, 博士研究員 (80351740)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000); Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2007: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2006: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 光物性 / 光合成 / たんぱく質構造 / 物性 / 光誘起協力現象 / エネルギー散逸 / たんぱく質構造・物性 / 蛋白質構造・物性

Research Abstract

紅色光合成細菌Rhodobacter sphaeroides 2.4.1由来の光反応中心複合体の1.95Åの高分解能X線結晶構造解析に日本で初めて成功した。その結果、光合成光保護メカニズムに関与すると考えられるカロテノイドの結合長の変位を検出するためには2.5Å以上の高分解能が必須であることがわかった。また分解能を左右するクライオプロテクションなどの操作を改良することにより、従来よりも小さい光を透過する結晶でも高分解能を得ることに成功した。

Research Products

• [Journal Article] Construction of Hybrid Photosynthetic Units Using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki, and H. Hashimoto,
  • Journal Title: Photosyn. Res 95 Pages: 327-337
  • Peer Reviewed
• [Journal Article] Probing the Effect of the Binding Site on the Electrostatic Behavior of a Series of Carotenoids Reconstituted into the Light-Harvesting 1 Complex from Purple Photosynthetic Bacterium Rhodospirillum rubrum Detected by Stark Spectroscopy (2008)
  • Author(s): K. Nakagawa S. Suzuki, R. Fujii, A. T. Gardiner, R. J. Cogdell, M. Nango, H. Hashimoto
  • Journal Title: J. Phys. Chem. B 112 Pages: 9467-9475
  • Peer Reviewed
• [Journal Article] 再構成LH1複合体中のスピリロキサンチンの電場変調吸収分光のX依存性 (2008)
  • Author(s): 堀部智子, 中川勝統, 楠本利行, 鈴木聡, 藤井律子, A.T. Gardiner, R.J. Cogdell, 南後守, 橋本秀樹
  • Journal Title: 光物性研究会論文集 19 Pages: 86-89
• [Journal Article] 紅色光合成細菌の光反応中心タンパク複合体のFT-IR分光測定 (2008)
  • Author(s): 柴田江身子, 藤井律子, 橋本秀樹
  • Journal Title: 光物性研究会論文集 19 Pages: 362-365
• [Journal Article] Characterization by Stark Spectroscopy of Reconstituted Light-Harvesting 1 Complex of Purple Photosynthetic Bacterium Rhodospirillum rubrum with the Polypeptides, Bacteriochlorophyll a, and Carotenoids (2008)
  • Author(s): K. Nakagawa, A. Mizuno, S.Suzuki, R. Fujii, T. Dewa, A.T. Gardiner, R.J. Cogdell, M. Nango, H. Hashimoto
  • Journal Title: Carotenoid Science 13 Pages: 38-45
  • Peer Reviewed
• [Journal Article] Coherent spectroscopy of carotenoid and bacteriochlorophyll (2008)
  • Author(s): M. Sugisaki, M. Fujiwara, K. Yanagi, R. Fujii, R. J. Cogdell, and H. Hashimoto
  • Journal Title: Photosynthesis.Energy of Sun: 14th International Congress on Photosynthesis Research 2007 (ed.J.F.Allen, et. al., Springer-Verlag, Berlin, Heidelberg, New York, Tokyo) Pages: 259-262
  • Peer Reviewed
• [Journal Article] Electrostatic effect of surfactant molecules on bacteriochlorophyll a and carotenoid binding site in the LH1 complex isolated from Rhodospirillum SI probed by Stark spectroscopy (2008)
  • Author(s): K. Nakagawa, S. Suzuki, R. Fujii, A. Gardiner, R. Cogdell, M. Nango, and H. Hashimoto
  • Journal Title: Photosynthesis Research 95 Pages: 345-351
  • Peer Reviewed
• [Journal Article] Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LHl complex from Rhodospirillum rubrum Sl by Stark spectroscopy (2008)
  • Author(s): K. Nakagawa, S. Suzuki, R. Fujii, A. Gardiner, R. Cogdell, M. Nango, and H. Hashimoto
  • Journal Title: Photosynthesis Research 95 Pages: 339-334
  • Peer Reviewed
• [Journal Article] Construction of hybrid photosynthetic units using peripheral and core antennae from two different species of photosynthetic bacteria: Detection of the energy transfer from bacteriochlorophyll a in LH2 to bacteriochlorophyll b in LHl (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki, and H. Hashimoto
  • Journal Title: Photosynthesis Research 95 Pages: 327-337
  • Peer Reviewed
• [Journal Article] Linear and nonlinear optical responses in bacteriochlorophyll a (2008)
  • Author(s): M. Sugisaki, R. Fujii, R. J. Cogdell, and H. Hashimoto
  • Journal Title: Photosynthesis Research 95 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Femtosecond Time-Resolved Absorption Spectra of Photosynthetic Membranes from a Purple Bacterium Blastochloris (Rhodopseudomonas) viridis (2007)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q.-D. Chen, M. Sugisaki, A. T. Gardiner, R. J. Cogdell, H. Hashimoto
  • Journal Title: Carotenoid Science 11 Pages: 26-31
  • Peer Reviewed
• [Journal Article] Selective Binding of Carotenoids with a Shorter Conjugated Chain to the LH2 Antenna Complex and those with a Longer Conjugated Chain to the Reaction Center from Rubrivivax gelatinosus (2007)
  • Author(s): Y. Kakitani, R. Fujii, Y. Hayakawa, M. Kurahashi, Y. Koyama, J. Harada, K. Shimada
  • Journal Title: Biochemistry 46 Pages: 7302-7313
  • Peer Reviewed
• [Journal Article] Symmetry Control of Radiative Decay in Linear Polyenes : Low Barriers for Isomerization in the S1 State of Hexadecaheptaene (2007)
  • Author(s): R. L. Christensen, M. G. I. Galinato, E. F. Chu, R. Fujii, H. Hashimoto, H. A. Frank
  • Journal Title: J. Am. Chem. Soc 129(6) Pages: 1769-1775
  • Peer Reviewed
• [Journal Article] Femtosecond time-resolved absorption spectra of photosynthetic membrane from apurple bacterium Blastochloris (Rhodopseudomonas) viridis (2007)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q. -D. Chen, M. Sugisaki, A. T. Gardiner, R. J. Cogdell. and H. Hashimoto
  • Journal Title: Carotenoid Science 11 Pages: 26-31
  • Peer Reviewed
• [Journal Article] The S_2 excited state and nonlinear optical effects in carotenoids investigated by femtosecond fluorescence and absorption spectroscopies (2007)
  • Author(s): M. Yoshizawa, D. Kosumi, M. Komukai, K. Yanagi. R. Fujii, and H. Hashimoto
  • Journal Title: Carotenoid Science 11 Pages: 21-25
  • Peer Reviewed
• [Journal Article] Triplet States of Carotenoids Having Shorter Conjugation Lengths Reconstituted into the Reaction Centre of Rhodobacter sphaeroides R26.1 Detected by Time-Resolved Absorption Spectroscopy (2006)
  • Author(s): R. Fujii, M. Shimizu, T. Nishio, T. Kusumoto, D. Niedzwiedzki, Z. Pendon, H. A. Frank, R. J. Cogdell, H. Hashimoto
  • Journal Title: Carotenoid Science 10 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Structures and Functions of Carotenoids Bound to Reaction Centres from Purple Photosynthetic Bacteria (2006)
  • Author(s): H. Hashimoto, R. Fujii, K. Yanagi, T. Kusumoto, A. T. Gardiner, A. W. Roszak, N. W. Issacs, Z. Pendon, D. Niedzwiedski, H. A. Frank, R. J. Cogdell
  • Journal Title: Pure Appl. Chem 78(8) Pages: 1505-1518
  • Peer Reviewed
• [Journal Article] Triplet-State Conformational Changes in 15-Cis-Spheroidene Bound to the Reaction Center from Rhodobacter sphaeroides 2.4.1 as Revealed by Time-Resolved EPR Spectroscopy : Strengthened Hypothetical Mechanism of Triplet-Energy Dissipation (2006)
  • Author(s): Y. Kakitani, R. Fujii, Y. Koyama, H. Nagae, L. Walker, B. Salter, A. Angerhofer
  • Journal Title: Biochemistry 45(7) Pages: 2053-2062
  • Peer Reviewed
• [Journal Article] Triplet-state conformational changes in 15-cis-spheroidene bound to the reaction center from Rhodobacter sphaeroides 2.4.1 as revealed by time-resolved EPR spectroscopy : Strengthened hypothetical mechanism of triplet-energy dissipation (2006)
  • Author(s): Y.Kakitani, R.Fujii, Y.Koyama, H.Nagae, L.Walker, B.Salter, A.Angerhofer
  • Journal Title: Biochemistry 45(7) Pages: 2053-2062
• [Journal Article] Structures and functions of carotenoids bound to reaction centres from purple photosynthetic bacteria (2006)
  • Author(s): H.Hashimoto, R.Fujii, K.Yanagi, T.Kusumoto, A.T.Gardiner, A.W.Roszak, N.W.Isaacs, Z.Pendon, D.Niedzwiedski, H.A.Frank, R.J.Cogdell
  • Journal Title: Pure and Applied Chemistry 78(8) Pages: 1505-1518
• [Journal Article] 光合成初期過程の最新の描像 (2006)
  • Author(s): 橋本秀樹, 藤井律子
  • Journal Title: 光合成微生物の機能と応用(上原 赫 編)((株)シーエムシー出版) 1章2節 Pages: 239-261
• [Presentation] 光合成細菌のアンテナ系LH1複合体のStark分光とカロテノイド分子の構造解析 (2009)
  • Author(s): 中川勝統, 堀部智子, 酒井俊亮, 中島彩乃, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 平成20年度生物物理学会中部支部講演会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2009-03-31
• [Presentation] オキナワモズク盤状体由来のフコキサンチン-クロロフィルa/c蛋白質(FCP)の単離 (2009)
  • Author(s): 喜多麻美子,藤井律子,伊波匡彦,橋本秀樹
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学(名古屋市) , ポスター
• [Presentation] Femtosecond Time-resolved Absorption Spectroscopy of a Polar Carotenoid Analo gue, 2-(all-trans-retinylidene) Indan-1, 3-dione in Various Solvents (2009)
  • Author(s): T. Kusumoto, D. Kosumi, T. Hashi moto, R. Fujii, M. Sugisaki, and H. Hashimoto
  • Organizer: Light-Harvesting Processes (LHP 2009)
  • Place of Presentation: Banz Monestary, Germany
• [Presentation] a-Conjugation Chain Length Dependence of the Dication State of Polar Carotenoid Analogues Measured by Nanosecond Time-Resolved Absorption Spectroscopy (2009)
  • Author(s): T. Kusumoto, R. Fujii, D. Kosumi and H. Hashimoto
  • Organizer: Light-Harvesting Processes (LHP 2009)
  • Place of Presentation: Banz Monestary, Germany
• [Presentation] Transient Grating Spectroscopy in Rhodobacter Sphaeroides 2.4.1 (2009)
  • Author(s): Y. Sugai, M. Sugisaki, M. Fujiwara, R. Fujii, R. J. Cogdell, and H. Hashimoto
  • Organizer: Light-Harvesting Processes (LHP 2009)
  • Place of Presentation: Banz Monestary, Germany
• [Presentation] オキナワモズク盤状体由来のフコキサンチン-クロロフィルa/c蛋白質(FCP)の単離 (2009)
  • Author(s): 喜多麻美子, 藤井律子, 伊波匡彦, 橋本秀樹
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学(名古屋市)
• [Presentation] 原子間力顕微鏡による光合成色素アンテナ系タンパク複合体を組み込んだ再構成膜のその場観察 (2009)
  • Author(s): 須貝祐子, 藤井律子, 杉崎満, 南後守, 橋本秀樹
  • Organizer: 日本化学会第89回春年会
  • Place of Presentation: 日本大学船橋キャンパス
• [Presentation] パルス繰り返しに同期したフェムト秒広帯域検出システムの構築と光合成初期過程への応用 (2009)
  • Author(s): 殿内規之, 須貝祐子, 藤井律子, 小澄大輔, 吉澤雅幸, 杉崎満, 橋本秀樹
  • Organizer: 日本物理学会第64回年次大会
  • Place of Presentation: 立教学院池袋キャンパス
• [Presentation] Structure of Reaction Centre Carotenoid in Blastochloris viridis (2008)
  • Author(s): A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄),招待講演
• [Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viridis (2008)
  • Author(s): A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
  • Organizer: The 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan,
• [Presentation] Femtosecond Time-Resolved Absorption Spectra of Photosynthetic Membranes from a Purple Bacterium Blastochloris (Rhodopseudomonas) viridis (2008)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q.-D. Chen, M. Sugisaki, A. T. Gardiner, R. J. Cogdell, H. Hashimoto
  • Organizer: 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Construction of Hybrid Photosynthetic Units using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki and H. Hashimoto
  • Organizer: 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] 紅色光合成細菌のアンテナ系LH1複合体の再構成とStark分光による機能評価 (2008)
  • Author(s): 中川勝統, 中野翼, 福井直美, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第8回日本光合成研究会公開シンポジウム
  • Place of Presentation: 名古屋大
• [Presentation] Construction of Hybrid Photosynthetic Units using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki and H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Probing the Effect of the Binding Site on the Electrostatic Behavior of A Series of Carotenoids Reconstituted into the Light-Harvesting 1 Complex from Purple P hotosynthetic Bacterium Rhodospirillum rubrum Detected by Stark Spectroscopy (2008)
  • Author(s): K. Nakagawa, N. Fukui, T. Nakano, T. Horibe, S. Suzuki, R. Fujii, A.T. Gardiner, R.J. Cogdell, H. Hashimo to and M. Nango
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Reconstitution of Antenna Light-Harvesting 1 Complexes from the Purple Photosynthetic Bacterium Rhodobacter sphaeroides with LH1-Polypeptides, Bacteriochlorophyll a, and Carotenoids : Carotenoid Specificity (2008)
  • Author(s): N. Fukui, A. Mizuno, T. Nakano, K. Nakagawa, A.T. Gardiner, R. J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Reconstitution of Light-Harvesting 1 Complexes from the Purple Photosynthetic Bacterium Rhodospirillum rubrum with Separately Isolated Polypeptides (LH1-a and LHI-β), Bacteriochlorophyll a, and Carotenoid (2008)
  • Author(s): T. Nakano, N. Fukui, K. Nakagawa, A.T.Gardiner, R.J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Transient Grating Spectroscopy in Rhodobacter sphaeroides 2.4.1 (2008)
  • Author(s): M. Sugisaki, M. Fujiwara, R. Fujii, K. Nakagawa, M. Nango, R.J. Cogdell, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Electroabsorption Spectroscopy of Native and Reconstituted LH1 Complexes from Purple Photosynthetic Bacterium Rhodospirillum rubrum (2008)
  • Author(s): T. Horibe, K. Nakagawa, T. Kusumoto, S. Suzuki, R. Fujii, A. T. Gardiner, R.J. Cogdell, M. Nango, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] π-Conjugation Chain Length Dependence of the Dication State of Polar Carotenoid Analogues Measured by Nanosecond Time-Resolved Absorption Spectroscopy (2008)
  • Author(s): T. Kusumoto, R. Fujii, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Femtosecond Time-Resolved Absorption Spectroscopy of a Polar Carotenoid Anal ogue, 2-(All-Trans-Retinylidene) Indan-1,3-Dione (2008)
  • Author(s): T. Kusumoto, T. Hashimoto, R. Fujii, M. Sugisaki, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Femtosecond Time-Resolved Absorption Spectra of Photosynthetic Membranes from a Purple Bacterium Blastochloris (Rhodopseudomonas) viridis (2008)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q.-D. Chen, M. Sugisaki, A.T. Gardiner, R.J. Cogdell, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] FT-IR Difference Spectrum of a 15-Cis Carotenoid Bound to the Reaction Centre from a Purple Photosynthetic Bacterium Rhodobacter sphaeroides (2008)
  • Author(s): E. Shibata, R. Fujii, H. Hashimoto
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viri dis, The 15th International Symposium on Carotenoids (2008)
  • Author(s): A.W. Roszak, A.T. Gardiner, V. Moulisova, A.D. Putranto, R. Fujii, H. Hashimoto, N.W. Isaacs, R.J. Cogdell
  • Organizer: 15^<th> International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
• [Presentation] 共役二重結合鎖数の異なるカロテノイドを用いた光合成細菌のアンテナ系LH1複合体の再構成とその評価 (2008)
  • Author(s): 中川勝統, 福井直美, 中野翼, 堀部智子, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] Rs. rubrumのアンテナ系タンパク質/色素複合体のカロテノイドを含む再構成 (2008)
  • Author(s): 中野翼, 福井直美, 中川勝統, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] Rb. Sphaeroides由来のアンテナ系タンパク質/色素複合体のカロテノイドを含む再構成 (2008)
  • Author(s): 福井直美, 中野翼, 中川勝統, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] 紅色光合成細菌Rs. rubrum S1株由来のLH1複合体の電場変調吸収分光測定 : χ依存性の測定 (2008)
  • Author(s): 堀部智子, 中川勝統, 楠本利行, 鈴木聡, 藤井律子, A.T. Gardiner, R.J. Cogdell, 南後守, 橋本秀樹
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] Rhodobacter sphaeroides 2.4.1における過渡回折格子信号 (2008)
  • Author(s): 杉崎満, 藤原正澄, 藤井律子, 中川勝統, 南後守, 橋本秀樹
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] 異種の光合成細菌由来のアンテナ及びコア複合体をもつ再構成光合成膜の創製 (2008)
  • Author(s): 藤井律子, 下中奨三, 内田直子, A.T. Gardiner, R.J. Cogdell, 杉崎満, 橋本秀樹
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] 紅色光合成細菌の光反応中心に結合した15-cisカロテノイドのFT-IR差スペクトル (2008)
  • Author(s): 柴田江身子, 藤井律子, 橋本秀樹
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] Structure of Reaction Centre Carotenoid in Blastochloris viridis (2008)
  • Author(s): A.W. Roszak, A.T. Gardiner, V. Moulisova, A.D. Putranto, R. Fujii, H. Hashimoto, N.W. Isaacs, R.J. Cogdell
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄)
• [Presentation] Transient Grating Spectroscopy in Photosynthetic Purple Bacteria Rhodobacter sphaeroides 2.4.1 (2008)
  • Author(s): M. Sugisaki, M. Fujiwara, R. Fujii, K. Nakagawa, M. Nango, R.J. Cogdell, and H. Hashimoto
  • Organizer: The 15^<th> International Conference on Luminescence and Optical Spectroscopy of Condensed Matter(ICL08)
  • Place of Presentation: Lyon, France
• [Presentation] Femtosecond Time-Resolved Absorption Spectroscopy of a Polar Carotenoid Anal ogue, 2-(All-Trans-Retinylidene) Indan-1, 3-Dione (2008)
  • Author(s): T. Kusumoto, T. Hashimoto, R. Fujii, M. Sugisaki, and H. Hashimoto
  • Organizer: The 15^<th> International Conference on Luminescence and Optical Spectroscopy of Condensed Matter (ICL08)
  • Place of Presentation: Lyon, France
• [Presentation] 光合成細菌のアンテナ系複合体でのカロテノイド分子結合サイトの検討 (2008)
  • Author(s): 中川勝統, 中野翼, 福井直美, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第18回バイオ・高分子シンポジウム
  • Place of Presentation: 上智大
• [Presentation] 光合成アンテナ系LHI複合体中のカロテノイド色素のプローブ効果 (2008)
  • Author(s): 中川勝統, 中野翼, 福井直美, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 2008年光化学討論会
  • Place of Presentation: 大阪府立大
• [Presentation] 光エネルギー変換素子としてのタンパク質/色素超分子複合体の構造形成と機能評価 (2008)
  • Author(s): 中川勝統, 中野翼, 福井直美, 出羽毅久, 藤井律子, 橋本秀樹, 南後守
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大
• [Presentation] 紅色光合成細菌Rb. sphaeroides2.4.1における縮退四光波信号 (2008)
  • Author(s): 杉崎満, 藤原正澄, 藤井律子, 橋本秀樹
  • Organizer: 日本物理学会第64回年次大会
  • Place of Presentation: 岩手大学上田キャンパス(盛岡市)
• [Presentation] 再構成LH1複合体中のスピリロキサンチンの電場変調吸収分光のx依存性 (2008)
  • Author(s): 堀部智子, 中川勝統, 楠本利行, 鈴木聡, 藤井律子, Alastair T. Gardiner, Richard J. Cogdell, 南後守, 橋本秀樹
  • Organizer: 第19回光物性研究会
  • Place of Presentation: 大阪市立大学(大阪市)
• [Presentation] 紅色光合成細菌の光反応中心タンパク複合体のFT-IR分光測定 (2008)
  • Author(s): 柴田江身子, 藤井律子, 橋本秀樹
  • Organizer: 第19回光物性研究会
  • Place of Presentation: 大阪市立大学(大阪市)
• [Presentation] 超高速レーザー分光で見えてきた光合成アンテナ色素蛋白複合体の機能 (2008)
  • Author(s): 橋本 秀樹・杉崎 満・藤原 正澄、藤井 律子、Cogdell R.J.
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学 池袋キャンパス
• [Presentation] 紅色光合成細菌のアンテナ系LH1複合体の構造形成とカロテノイド色素の機能評価 (2008)
  • Author(s): 中川 勝統、水野 愛弓、中野 翼、福井 直美、出羽 毅久、藤井 律子、橋本 秀樹、南後 守
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学池袋キャンパス
• [Presentation] Construction of Hybrid Photosynthetic Units using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2007)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki and H. Hashimoto
  • Organizer: 14th International Congress of Photosynthesis : Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Buchanan Arms Hotel, Drymen, UK
• [Presentation] 人工膜内に配列した異種の光合成色素蛋白複合体間のエネルギー伝達 (2007)
  • Author(s): 藤井律子,下中奨三,内田直子,A. T. Gardiner,R. J. Cogdell,杉崎満,橋本秀樹
  • Organizer: 日本物理学会2007年春季大会
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島市)
• [Presentation] Reconstitution of Light-harvesting l complex of photosynthetic bacterium Rhodospirillum rubrum with the LHl subunit-type complex and carotenoids and itscharacterization (2007)
  • Author(s): K. Nakagawa, S. Suzuki, A. Mizuno, R. Fujii, A. T. Gardiner, RJ. Cogdell, H. Hashimoto, M. Nango
  • Organizer: 2nd lnternational Workshop of Photosynthetic Antennae and Coherent Phenomena
  • Place of Presentation: Osaka, Japan
• [Presentation] β-Conjugated Chain Length Dependence of the dication state of Polar Carotenoid Analogues Measured by Nanosecond Time-Resolved Absorption Spectroscopy (2007)
  • Author(s): Toshiyuki Kusumoto, Ritsuko Fujii, and Hideki Hashimoto
  • Organizer: 2nd International Workshop of Photosynthetic Antennae and Cherent Phenomena
  • Place of Presentation: Osaka, Japan
• [Presentation] 非共鳴励起によるカロテノイド類の超高速光学応答と多光子励起過程 (2007)
  • Author(s): 小澄 大輔, 藤原 正澄, 藤井 律子, 橋本 秀樹, 吉澤 雅幸
  • Organizer: 第62回日本物理学会
  • Place of Presentation: 北海道大学
• [Presentation] バクテリオクロロフィルaの超高速光学応答II (2007)
  • Author(s): 杉崎 満, 藤井 律子, 橋本 秀樹
  • Organizer: 第62回日本物理学会
  • Place of Presentation: 北海道大学
• [Presentation] 非共鳴励起によるカロテノイド類の超高速光学応答と多光子励起過程 (2007)
  • Author(s): 小澄 大輔, 藤原 正澄, 藤井 律子, 橋本 秀樹, 吉澤 雅幸
  • Organizer: 第62回目本物理学会
  • Place of Presentation: 北海道大学
• [Presentation] 極性カロテノイド同属体のナノ秒時間分解吸収分光 (2007)
  • Author(s): 楠本 利行, 藤井 律子, 橋本 秀樹
  • Organizer: 第62回日本物理学会
  • Place of Presentation: 北海道大学
• [Presentation] カロテノイド類における超高速励起状態ダイナミクスの共役長依存性 (2007)
  • Author(s): 小澄 大輔, 阿部 健太, 藤原 正澄, 藤井 律子, 橋本 秀樹, 吉澤 雅幸
  • Organizer: 第1回分子科学討論会
  • Place of Presentation: 仙台
• [Presentation] 紅色光合成細菌のアンテナ系タンパク質/色素複合体(LHI)のタンパク質とカロテノイドとの再構成とその評価 (2007)
  • Author(s): 中川 勝統, 鈴木 聡, 水野 愛弓, 福井 直美, 中野 翼, 藤井 律子, 出羽 毅久, 橋本 秀樹, 南後 守
  • Organizer: 第21回カロテノイド研究談話会
  • Place of Presentation: 大阪市立大学
• [Presentation] 極性カロテノイド同属体のナノ秒時間分解吸収分光 (2007)
  • Author(s): 楠本 利行, 藤井 律子, 橋本 秀樹
  • Organizer: 第21回カロテノイド研究談話会
  • Place of Presentation: 大阪市立大学
• [Presentation] Coherent Spectroscopy in Carotenoid and Bacteriochlorophyll (2007)
  • Author(s): Mitsuru Sugisaki, Masazumi Fujiwara, Kazuhiro Yanagi, Ritsuko Fujii, Richard J. Cogdell, and HidekiHashimoto
  • Organizer: 14th International Congress on Photosynthesis
  • Place of Presentation: Glasgow, Scotland
• [Presentation] Probing the carotenoid in its binding site in a Reconstituted LH1 complex fromthe photosynthetic Bacterium Rhodospirillum rubrum with electroabsorption spectroscopy (2007)
  • Author(s): K. Nakagawa, S. Suzuki, R. Fujii, A. T .Gardiner, R. J. Cogdell, H. Hashimoto, M. Nango
  • Organizer: 14th International Congress on Photosynthesis
  • Place of Presentation: Glasgow, Scotland
• [Presentation] Reconstitution of carotenoids into light-harvesting 1 complex of purple bacterium Rhodospirillum rubrum and analysis of electrostatic environment of pigments in the complexes (2007)
  • Author(s): K. Nakagawa, S. Suzuki, R. Fujii, A. T. Gardiner, R. J. Cogdell, H. Hashimoto, M. Nango
  • Organizer: Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Drymen, Scotland
• [Presentation] Sub-20 fs coherent spectroscopy of photosynthetic pigments (2007)
  • Author(s): M. Sugisaki, R. Fujii, R. J. Cogdell and H. Hashimoto
  • Organizer: Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Drymen, Scotland
• [Presentation] Construction of hybrid photosynthetic units using peripheral and core antennae from two different species of photosynthetic bacteria:Detection of the energy transfer from bacteriochlorophyll a in LH2 to bacteriochlorophyll b in LHl (2007)
  • Author(s): R. Fujii, S. shimonaka, N. Uchida, A. T. Gardiner, R. J.Cogdell, M. Sugisaki, and H. Hashimoto
  • Organizer: Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Drymen, Scotland
• [Presentation] Vibronic Coherence of Photosynthetic Pigments (2007)
  • Author(s): M. Sugisaki, M. Fujiwara, R. Fujii, K. Nakagawa, M. Nango, R. J. Cogdell, and H. Hashimoto
  • Organizer: The 15t International Conference on Luminescence and Optical Spectroscopy of Condensed Matter (ICL'08)
  • Place of Presentation: Lyon, France
• [Presentation] 人工膜内に配列した異種の光合成色素蛋白複合体間のエネルギー伝達 (2006)
  • Author(s): 藤井律子,下中奨三,内田直子,A. T.Gardiner,R. J. Cogdell,杉崎満,橋本秀樹
  • Organizer: 第17 回光物性研究会
  • Place of Presentation: 大阪市立大学(大阪)
• [Presentation] Heterogeneous Two-Dimensional-Crystallization and Energy Transfer of the LH2 and LH1-RC Complexes from Different Species of Photosynthetic Bacteria : An Investigation by Means of Electron Microscopy and Near-Infrared Fluorescence Spectroscopy (2006)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki And H. Hashimoto
  • Organizer: 12th International Symposium of Phototrophic Prokaryotes
  • Place of Presentation: Pau(south of France)
• [Book] In : Petra Fromme (Ed.), Photosynthetic Protein Complexes A Structural Approach (2008)
  • Author(s): R. J. Cogdell, A. T. Gardiner, M. Gabrielsen, J. Southall, A. W. Roszak, N. W. Isaacs, R. Fujii and H. Hashimoto, Wiley-VCH Verlag GmbH & Co. KGaA,
  • Publisher: The Structure of Purple Bacterial Antenna Complexes
• [Book] Petra Fromme (Ed.), Photosynthetic Protein Complexes A Structural Approach (2008)
  • Author(s): R.J. Cogdell, A.T. Gardiner, M. Gabrielsen, J. Southall, A.W. Roszak, N.W. Isaacs, R. Fujii, H. Hashimoto
  • Publisher: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
• [Book] 光合成微生物の機能と応用(上原赫編)1章2節 (2006)
  • Author(s): 橋本秀樹,藤井律子
  • Publisher: (株)シーエムシー出版,「光合成の初期過程の最新の描像」
• [Book] 光物性の基礎と応用(光物性研究会組織委員会編)第3部5章 (2006)
  • Author(s): 橋本秀樹,藤井律子,杉崎満
  • Publisher: オプトロニクス社,「有機・バイオ-光合成アンテナ色素淡白複合体・カロテノイド・超高速レーザー分光-」
• [Book] In : B. Grimm, R. Porra, W. Rudiger and H. Scheer (eds.), Chlorophylls and Bacteriochlorophylls : Biochemistry, Biophysics, Functions and Applications (Advances in Photosynthesis and Respiration, Vol. 25) (2006)
  • Author(s): Y. Koyama, Y. Kakitani, L. Limantara and R. Fujii, Springer
  • Publisher: Effects of Axial Coordination, Electronic Excitation and Oxidation on Bond Orders in the Bacteriochlorin Macrocycle, and Generation of Radical Cation on Photo-Excitation of in vitro and in vivo Bacteriochlorophyll a Aggregates : Resonance Raman Study