Development of novel methods for efficient construction of functional RNAs

• Project/Area Number: 18685020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Kyushu University
• Principal Investigator: IKAWA Yoshiya Kyushu University, 大学院・工学研究院, 准教授 (70281087)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2007: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2006: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 生体関連高分子化学 / 進化工学 / RNA / リボザイム / デザイン / モジュール / BODIPY / 蛍光標議 / ポリメラーゼ

Research Abstract

「人工酵素などの機能性生体高分子のテーラーメイドな創製」は、RNAに限らず、広く生体高分子研究において、基礎化学/生物学的に、また医療やバイオ工学などへの応用面からも重要な課題である。本研究では、研究代表者の考案した新手法「design & selection法」を基盤とする人工リボザイムの創製、機能改変、機能解析に取り組み、本手法の確立を行い、その有効性を示した。

Research Products

• [Journal Article] Tailoring RNA modular units on a common scaffold : a modular ribozyme with a catalytic unit for β-nicotinamide mononucleotide-activated RNA ligation. (2009)
  • Author(s): Y. Fujita, et.al.
  • Journal Title: RNA 14(In press)
  • Peer Reviewed
• [Journal Article] Concerted effects of two activator modules on the group I ribozyme reaction (2009)
  • Author(s): Y. Ikawa, et.al.
  • Journal Title: Journal of Biochemistry 139(In press)
  • NAID: 10026301931
  • Peer Reviewed
• [Journal Article] N-confused porphyrin possessing glucamine-appendants : aggregation and acid/base properties in aquaous media (2008)
  • Author(s): Ikawa. Y., Ogawa, H., Harada, H., & Furuta, H
  • Journal Title: Bioorg. Med. Chem. Lett 18 Pages: 6394-6397
• [Journal Article] Acid-base properties and DNA-binding of water soluble N-confused porphyrins with cationic side-arms (2008)
  • Author(s): Ikawa. Y., Moriyama, S., Harada, H., & Furuta, H
  • Journal Title: Org. Biomol. Chem 6 Pages: 4157-4166
• [Journal Article] Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme (2008)
  • Author(s): Ishikawa, J., Furuta. H., & Ikawa, Y.
  • Journal Title: Nucleic Acids Symp. Ser 52 Pages: 523-524
• [Journal Article] Facile syntheses of BODIPY derivatives for fluorescent labeling of the 5' and 3' ends of RNAs (2008)
  • Author(s): Ikawa. Y., Moriyama S., & Furuta, H
  • Journal Title: Anal. Biochem 378 Pages: 166-170
• [Journal Article] Selection of a novel class of RNA-RNA interaction motifs based on the ligase ribozyme with defined modular architecture (2008)
  • Author(s): Ohuchi, SP., Ikawa, Y., & Nakamura Y.
  • Journal Title: Nucleic Acids Res 36 Pages: 3600-3607
• [Journal Article] Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme (2008)
  • Author(s): J. Ishikawa, et.al.
  • Journal Title: Nucleic Acids Symp. Ser 52 Pages: 523-524
• [Journal Article] Slection of a novel class of RNA-RNA interaction motifs based on the ligase ribozyme with defined modular architecture (2008)
  • Author(s): S, Ohuchi, et. al.
  • Journal Title: Nucleic Acids Research 36(In press)
  • Peer Reviewed
• [Journal Article] Facile syntheses of BODIPY derivatives for fluorescent labeling of the 3' and 5' ends of RNAs (2008)
  • Author(s): Y, Ikawa, et. al.
  • Journal Title: Analytical Biochemistry 377(In press) Pages: 8-8
  • Peer Reviewed
• [Journal Article] Design and analysis of a structural RNA that acts as a template for peptide ligation (2007)
  • Author(s): Kashiwagi, N., Furuta. H., & Ikawa, Y
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 387-388
• [Journal Article] Synthesis of a water soluble N-confused porphyrin and its interaction with nucleic acids (2007)
  • Author(s): Moriyama, S., Ikawa. Y., & Furuta, H
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 207-208
• [Journal Article] Synthesis of a water soluble N-confused porphyrin and its interaction with nucleic acids. (2007)
  • Author(s): S, Moriyama, et. al.
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 2007-2008
• [Journal Article] 人工RNA触媒創製の新手法 : de novoデザインと進化分子工学の融合. (2006)
  • Author(s): Ikawa, Y.
  • Journal Title: 蛋白質核酸酵素 51 Pages: 950-957
  • NAID: 40007357934
• [Journal Article] Construction of an artificial ribozyme which ligates an RNA fragment activated by nicotinamide mononucleotide (2006)
  • Author(s): Fujita, Y., Furuta. H., & Ikawa, Y
  • Journal Title: Nucleic Acids Symp. Ser 50 Pages: 349-350
• [Journal Article] 人工RNA創製の新手法 (2006)
  • Author(s): 井川善也
  • Journal Title: 蛋白質核酸素 51 Pages: 950-957
• [Journal Article] Structural and biochemical characterization of DSL ribzyme (2006)
  • Author(s): Souta Horie et al.
  • Journal Title: Biochem. Biophys. res. Comm 339 Pages: 115-121
• [Journal Article] Construction of an artificial ribozyme which ligase an RNA fragment activated by nicotinamide mononucleotide (2006)
  • Author(s): Yuki Fujita et al.
  • Journal Title: Nucleic Acids Symp. Ser. 50 Pages: 231-232
• [Presentation] RNA Science & Technology through Design & Evolution (2008)
  • Author(s): Yoshiya Ikawa
  • Organizer: Prof. Jean-Marie Lehn Symposium III
  • Place of Presentation: Nishijin Plaza, Fukuoka, Janan
  • Year and Date: 2008-10-17
• [Presentation] RNAとペプチドのcross-catalysisモデル構築へ向けた試み (2008)
  • Author(s): Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第10回生命化学研究会シンポジム
  • Place of Presentation: 熊本
  • Year and Date: 2008-01-11
• [Presentation] High Turnover Ability of a Trans-Acting RNA Ligase Ribozyme Developed through Modular Engineering (2008)
  • Author(s): Junya Ishikawa, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: Thirteenth Annual Meeting of the RNA Society
  • Place of Presentation: Berlin
• [Presentation] Tailoring RNA Modular Units on a Common Scaffold : A Modular Ribozyme Having a Catalytic Unit for b-NMN Activated RNA Ligation (2008)
  • Author(s): Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: Thirteenth Annual Meeting of the RNA Society
  • Place of Presentation: Berlin
• [Presentation] ユニット集積型人工リガーゼ・リボザイムの構造・機能相関解析 (2008)
  • Author(s): Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
• [Presentation] Tailoring RNA modular units on a common scaffold : a modular ribozyme havina a catalytic unit for b-NMN activated RNA ligation (2008)
  • Author(s): Y. Ikawa, et.al.
  • Organizer: RNA society meeting 2008
  • Place of Presentation: Free Uiv. of Berlin (Germany)
• [Presentation] デザイン型RNA上でRNP複合体形成を介する効率的ペプチド連結反応 (2007)
  • Author(s): Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
  • Place of Presentation: 横浜
• [Presentation] モジュール工学によるDSLリボザイムの構造・活性相関の解析 (2007)
  • Author(s): Junya Ishikawa, Shohei Kuramitsu, Hiroyuki Furuta, Tan Inoue, Yoshiya Ikawa
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
  • Place of Presentation: 横浜
• [Presentation] 小型化と高性能化に向けたユニット集積型人工リガーゼ・リボザイムの構造解析 (2007)
  • Author(s): Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
  • Place of Presentation: 横浜
• [Presentation] Design and analysis of a structural RNA that acts as a template for peptide ligation (2007)
  • Author(s): Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo
• [Presentation] Junya Ishikawa, Shohei Kuramitsu, Hiroyuki Furuta, Tan Inoue, Yoshiya Ikawa (2007)
  • Author(s): テトラループ・レセプター・ユニットを標的としたDSLリボザイムのモジュール工学
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] ユニット集積型人工リガーゼ・リボザイムの構造・機能相関解析 (2007)
  • Author(s): Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] RNP形成によりペプチド連鎖反応を促進するデザイン型RNA構造体の作成と解析 (2007)
  • Author(s): Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Modular Design & Evolution of RNA/RNP Catalysts (2007)
  • Author(s): Yoshiya Ikawa, Norimasa Kashiwagi, Yuki Fujita, Hiroyuki Furuta
  • Organizer: Synthetic Biology 3.0
  • Place of Presentation: Zurich, Switzerland
• [Presentation] Toward Construction of Mg2+-Free Catalytic Modules for RNA Ligase Ribozymes (2007)
  • Author(s): Kuniyasu Kumon, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 12th Annual Meeting of the RNA Society
  • Place of Presentation: Madison, Wisconsin
• [Presentation] Effective Peptide Ligation on 3D-RNA through Formation of a Ribonucleo-Peptide Complex (2007)
  • Author(s): Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 12th Annual Meeting of the RNA Society
  • Place of Presentation: Madison, Wisconsin
• [Presentation] A Novel RNA Catalyzing RNA-RNA Ligation Activated by β-NMN (2007)
  • Author(s): Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
  • Organizer: 12th Annual Meeting of the RNA Society
  • Place of Presentation: Madison, Wisconsin
• [Presentation] (2007)
  • Author(s): Y, Ikawa
  • Organizer: RNA society meeting 2007
  • Place of Presentation: U. of Wisconsin, Madison(USA)
• [Presentation] Y. Fujita, Y. Ikawa, H. Furuta (2006)
  • Author(s): A novel RNA enzyme catalyzing RNA-RNA ligation activated by b-NMN
  • Organizer: RNA 2006
  • Place of Presentation: Izu
• [Presentation] Effective peptide ligation on a 3D-RNA through formation of a ribonucleo-peptide complex (2006)
  • Author(s): N. Kashiwagi, Y. Ikawa, H. Furuta
  • Organizer: RNA 2006
  • Place of Presentation: Izu
• [Book] ed. Silverman, SK., Landes Bioscience (2006)
  • Author(s): Inoue, T., & Ikawa, Y
  • Publisher: Protein switched ribozymes. in Nucleic Acid Switches and Sensors
• [Remarks]
  • URL: http://www.cstf.kyushu-u.ac.jp/~furutalab/