Secreted peptidomics focused on posttransiational modifications

• Project/Area Number: 18687003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Nagoya University
• Principal Investigator: MATSUBAYASHI Yoshikatsu Nagoya University, 大学院・生命農学研究科, 准教授 (00313974)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000); Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2006: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: 分泌型ペプチド / ペプチドホルモン / 翻訳後修飾 / 受容体キナーゼ / 細胞間情報伝達 / リガンド-受容体相互作用 / チロシン硫酸化 / リガンドー受容体相互作用 / 網羅的解析

Research Abstract

植物のかたちづくりには様々な内生のペプチドホルモンが関与している.本研究では,in silicoおよび生化学的解析を統合して,翻訳後修飾ペプチドホルモン候補を探索し,その生理機能解梼を行なった.その結果,複数の新規ペプチドホルモン候補を見出すことに成功した,また,翻訳後修飾のひとつであるチロシン硫酸化酵素の精製とクローニングを行ない,その遺伝子破壊株が様々な成長異常を示すことを見出した.

Research Products

• [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis. (2009)
  • Author(s): Komori, R., Amano, Y., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 15067-15072
• [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana. (2009)
  • Author(s): Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Nature Chem Biol 5 Pages: 578-580
• [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. (2008)
  • Author(s): Ogawa, M., Shinohara, H., Sakagami, Y., Matsubayashi, Y.
  • Journal Title: Science 319 Pages: 294-294
  • NAID: 130006995152
• [Journal Article] Non-cell-autonomous control of vascular stein cell fate by a CLE peptide/receptor system. (2008)
  • Author(s): Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y., Fukuda, H.
  • Journal Title: Proc Nail Acad Sci USA 105 Pages: 15208-15213
• [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Ohyama, K., Ogawa, M., Matsubayashi, Y.
  • Journal Title: Plant J. 55 Pages: 152-160
• [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Ohyama K., et al.
  • Journal Title: Plant J. 55 Pages: 152-160
  • Peer Reviewed
• [Journal Article] Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. (2008)
  • Author(s): Hirakawa Y., et al.
  • Journal Title: Proc.Acad.Natl.Sci.USA 105 Pages: 15208-15213
  • Peer Reviewed
• [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis. (2008)
  • Author(s): Komori R., et al.
  • Journal Title: Proc.Acad.Natl.Sci.USA 106 Pages: 15067-15072
  • Peer Reviewed
• [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. (2008)
  • Author(s): Ogawa M., et. al.
  • Journal Title: Science 319 Pages: 294-294
  • Peer Reviewed
• [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. (2007)
  • Author(s): Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 18333-18338
• [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. (2007)
  • Author(s): Shinohara, H., Ogawa, M., Sakagami, Y., Matsubayashi, Y.
  • Journal Title: J Biol Chem 282 Pages: 124-131
• [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing. (2007)
  • Author(s): Shinohara, H., Matsubayashi, Y.
  • Journal Title: Plant J 52 Pages: 175-184
  • NAID: 130006995151
• [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. (2007)
  • Author(s): Amano Y., et. al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 18333-18338
  • Peer Reviewed
• [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeadstowards receptor array construction and receptor-based ligand fishing. (2007)
  • Author(s): Shinohara H., et. al.
  • Journal Title: Plant J. 52 Pages: 175-184
  • Peer Reviewed
• [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. (2007)
  • Author(s): Shinohara H. et al.
  • Journal Title: J. Biol. Chem. 282(1) Pages: 124-131
• [Journal Article] Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth. (2006)
  • Author(s): MatsuoayaShi Y. et al.
  • Journal Title: Plant Physiol. 142(1) Pages: 45-53
• [Journal Article] Peptide hormones in plants. (2006)
  • Author(s): Matsubayashi Y. et al.
  • Journal Title: Annu.Rev. Plant Biol. 57 Pages: 649-674
• [Presentation] 翻訳後修飾に着目した新規ペプチドホルモン候補探索 (2009)
  • Author(s): 松林嘉克
  • Organizer: 日本化学会第89春期年会シンポジウム"ケミカルバイオロジー研究の最前線"
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-27
• [Presentation] In silico遺伝子スクリーニングによって見出された新規ペプチドホルモン候補 (2009)
  • Author(s): 松崎曜, ら
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-20
• [Presentation] 植物培養細胞リソースを用いたペプチドホルモン探索 (2008)
  • Author(s): 松林嘉克
  • Organizer: 文部科学省ナショナルバイオリソースプロジェクトシンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] シロイヌナズナにおけるチロシン硫酸化酵素の精製 (2008)
  • Author(s): 小森領太, ら
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] リガンド-受容体ペアの同定から迫る植物のかたちづくり (2008)
  • Author(s): 松林嘉克
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 分泌型ペプチドの選択的抽出・解析法と新規ペプチドホルモン探索への応用 (2008)
  • Author(s): 松林嘉克
  • Organizer: 植物ホルモン技術ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-28
• [Presentation] Novel approaches to identify peptide hormone candidates in Arabidopsis. (2008)
  • Author(s): Matsubayashi Y.
  • Organizer: 55th NIBB Conference "Frontiers of Plant Science in the 21st Century"
  • Place of Presentation: 岡崎
  • Year and Date: 2008-09-09
• [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis. (2008)
  • Author(s): Ohyama K., et al.
  • Organizer: 19th International Conference on Arabidopsis Research
  • Place of Presentation: カナダ,モントリオール
  • Year and Date: 2008-07-23
• [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis. (2008)
  • Author(s): Ohyama K., et al.
  • Organizer: Japan-Korea Symposium "Plant Growth and Signal Transduction"
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-09
• [Presentation] イネ細胞培養液に含まれる分泌型ペプチドの解析 (2008)
  • Author(s): 小木綾子, ら
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-28
• [Presentation] 受容体の固相固定化を基盤とした新しいリガンドー受容体相互作用検出系 (2008)
  • Author(s): 篠原秀文, ら
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-22
• [Presentation] CLV3ペプチドはCLV1受容体キナーゼ細胞外領域に直接結合する (2008)
  • Author(s): 小川真理, ら
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-22
• [Presentation] 植物細胞培養液を用いた分泌型ペプチドミクス (2008)
  • Author(s): 大山健太郎, ら
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-22
• [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Matsubayashi, Y., Ohyama, K., Ogawa, M.
  • Organizer: Japan-Korea Symposium-Plant Growth and Signal Transduction.
  • Place of Presentation: Yokohama, Japan
• [Presentation] Roles of two structurally distinct sulfated peptides, PSK and PSYl, in Arabidopsis growth and development. (2007)
  • Author(s): Matsubayashi Y.
  • Organizer: 19th IPGSA meeting
  • Place of Presentation: メキシコ,プエルトバジャルタ
  • Year and Date: 2007-07-24
• [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development. (2007)
  • Author(s): Matsubayashi Y.
  • Organizer: 18th International Conference on Arabidopsis Research.
  • Place of Presentation: 中国,北京
  • Year and Date: 2007-06-23
• [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development. (2007)
  • Author(s): Matsubayashi Y.
  • Organizer: 18th International Conference on Arabidopsis Research.
  • Place of Presentation: Beijing, China
• [Book] ペプチペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり. 蛋白質核酸酵素 (2009)
  • Author(s): 松林嘉克
  • Publisher: 秀潤社
• [Book] リガンドー受容体ペアの解析から探る植物の細胞間シグナリング. 化学と生物 (2008)
  • Author(s): 松林嘉克, 篠原秀文
  • Publisher: 学会出版センター
• [Book] Handbook of Biologically Active Peptides. Chapter 6 Phytosulfokine. (2006)
  • Author(s): Matsubayashi Y. et al.
  • Total Pages: 1595
  • Publisher: Academic Press
• [Remarks]
  • URL: http://www.agr.nagoya-u,ac.jp/~bioact/matsu/index.html
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~bioact/
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~bioact/