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Secreted peptidomics focused on posttransiational modifications

Research Project

Project/Area Number 18687003
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field 植物生理・分子
Research InstitutionNagoya University

Principal Investigator

MATSUBAYASHI Yoshikatsu  Nagoya University, 大学院・生命農学研究科, 准教授 (00313974)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2006: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords分泌型ペプチド / ペプチドホルモン / 翻訳後修飾 / 受容体キナーゼ / 細胞間情報伝達 / リガンド-受容体相互作用 / チロシン硫酸化 / リガンドー受容体相互作用 / 網羅的解析
Research Abstract

植物のかたちづくりには様々な内生のペプチドホルモンが関与している.本研究では,in silicoおよび生化学的解析を統合して,翻訳後修飾ペプチドホルモン候補を探索し,その生理機能解梼を行なった.その結果,複数の新規ペプチドホルモン候補を見出すことに成功した,また,翻訳後修飾のひとつであるチロシン硫酸化酵素の精製とクローニングを行ない,その遺伝子破壊株が様々な成長異常を示すことを見出した.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (40 results)

All 2009 2008 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 6 results) Presentation (17 results) Book (3 results) Remarks (3 results)

  • [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis.2009

    • Author(s)
      Komori, R., Amano, Y., Ogawa-Ohnishi, M., Matsubayashi, Y.
    • Journal Title

      Proc Natl Acad Sci USA 106

      Pages: 15067-15072

    • Related Report
      2008 Final Research Report
  • [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana.2009

    • Author(s)
      Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M., Matsubayashi, Y.
    • Journal Title

      Nature Chem Biol 5

      Pages: 578-580

    • Related Report
      2008 Final Research Report
  • [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain.2008

    • Author(s)
      Ogawa, M., Shinohara, H., Sakagami, Y., Matsubayashi, Y.
    • Journal Title

      Science 319

      Pages: 294-294

    • NAID

      130006995152

    • Related Report
      2008 Final Research Report
  • [Journal Article] Non-cell-autonomous control of vascular stein cell fate by a CLE peptide/receptor system.2008

    • Author(s)
      Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y., Fukuda, H.
    • Journal Title

      Proc Nail Acad Sci USA 105

      Pages: 15208-15213

    • Related Report
      2008 Final Research Report
  • [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama, K., Ogawa, M., Matsubayashi, Y.
    • Journal Title

      Plant J. 55

      Pages: 152-160

    • Related Report
      2008 Final Research Report
  • [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Journal Title

      Plant J. 55

      Pages: 152-160

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system.2008

    • Author(s)
      Hirakawa Y., et al.
    • Journal Title

      Proc.Acad.Natl.Sci.USA 105

      Pages: 15208-15213

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis.2008

    • Author(s)
      Komori R., et al.
    • Journal Title

      Proc.Acad.Natl.Sci.USA 106

      Pages: 15067-15072

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain.2008

    • Author(s)
      Ogawa M., et. al.
    • Journal Title

      Science 319

      Pages: 294-294

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis.2007

    • Author(s)
      Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M., Matsubayashi, Y.
    • Journal Title

      Proc Natl Acad Sci USA 104

      Pages: 18333-18338

    • Related Report
      2008 Final Research Report
  • [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling.2007

    • Author(s)
      Shinohara, H., Ogawa, M., Sakagami, Y., Matsubayashi, Y.
    • Journal Title

      J Biol Chem 282

      Pages: 124-131

    • Related Report
      2008 Final Research Report
  • [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing.2007

    • Author(s)
      Shinohara, H., Matsubayashi, Y.
    • Journal Title

      Plant J 52

      Pages: 175-184

    • NAID

      130006995151

    • Related Report
      2008 Final Research Report
  • [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis.2007

    • Author(s)
      Amano Y., et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 18333-18338

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeadstowards receptor array construction and receptor-based ligand fishing.2007

    • Author(s)
      Shinohara H., et. al.
    • Journal Title

      Plant J. 52

      Pages: 175-184

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling.2007

    • Author(s)
      Shinohara H. et al.
    • Journal Title

      J. Biol. Chem. 282(1)

      Pages: 124-131

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth.2006

    • Author(s)
      MatsuoayaShi Y. et al.
    • Journal Title

      Plant Physiol. 142(1)

      Pages: 45-53

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Peptide hormones in plants.2006

    • Author(s)
      Matsubayashi Y. et al.
    • Journal Title

      Annu.Rev. Plant Biol. 57

      Pages: 649-674

    • Related Report
      2006 Annual Research Report
  • [Presentation] 翻訳後修飾に着目した新規ペプチドホルモン候補探索2009

    • Author(s)
      松林嘉克
    • Organizer
      日本化学会第89春期年会シンポジウム"ケミカルバイオロジー研究の最前線"
    • Place of Presentation
      船橋
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] In silico遺伝子スクリーニングによって見出された新規ペプチドホルモン候補2009

    • Author(s)
      松崎曜, ら
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 植物培養細胞リソースを用いたペプチドホルモン探索2008

    • Author(s)
      松林嘉克
    • Organizer
      文部科学省ナショナルバイオリソースプロジェクトシンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナにおけるチロシン硫酸化酵素の精製2008

    • Author(s)
      小森領太, ら
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] リガンド-受容体ペアの同定から迫る植物のかたちづくり2008

    • Author(s)
      松林嘉克
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 分泌型ペプチドの選択的抽出・解析法と新規ペプチドホルモン探索への応用2008

    • Author(s)
      松林嘉克
    • Organizer
      植物ホルモン技術ワークショップ
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Novel approaches to identify peptide hormone candidates in Arabidopsis.2008

    • Author(s)
      Matsubayashi Y.
    • Organizer
      55th NIBB Conference "Frontiers of Plant Science in the 21st Century"
    • Place of Presentation
      岡崎
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Organizer
      19th International Conference on Arabidopsis Research
    • Place of Presentation
      カナダ,モントリオール
    • Year and Date
      2008-07-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Organizer
      Japan-Korea Symposium "Plant Growth and Signal Transduction"
    • Place of Presentation
      横浜
    • Year and Date
      2008-06-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネ細胞培養液に含まれる分泌型ペプチドの解析2008

    • Author(s)
      小木綾子, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受容体の固相固定化を基盤とした新しいリガンドー受容体相互作用検出系2008

    • Author(s)
      篠原秀文, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] CLV3ペプチドはCLV1受容体キナーゼ細胞外領域に直接結合する2008

    • Author(s)
      小川真理, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] 植物細胞培養液を用いた分泌型ペプチドミクス2008

    • Author(s)
      大山健太郎, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.2008

    • Author(s)
      Matsubayashi, Y., Ohyama, K., Ogawa, M.
    • Organizer
      Japan-Korea Symposium-Plant Growth and Signal Transduction.
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Roles of two structurally distinct sulfated peptides, PSK and PSYl, in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      19th IPGSA meeting
    • Place of Presentation
      メキシコ,プエルトバジャルタ
    • Year and Date
      2007-07-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      18th International Conference on Arabidopsis Research.
    • Place of Presentation
      中国,北京
    • Year and Date
      2007-06-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      18th International Conference on Arabidopsis Research.
    • Place of Presentation
      Beijing, China
    • Related Report
      2008 Final Research Report
  • [Book] ペプチペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり. 蛋白質核酸酵素2009

    • Author(s)
      松林嘉克
    • Publisher
      秀潤社
    • Related Report
      2008 Final Research Report
  • [Book] リガンドー受容体ペアの解析から探る植物の細胞間シグナリング. 化学と生物2008

    • Author(s)
      松林嘉克, 篠原秀文
    • Publisher
      学会出版センター
    • Related Report
      2008 Final Research Report
  • [Book] Handbook of Biologically Active Peptides. Chapter 6 Phytosulfokine.2006

    • Author(s)
      Matsubayashi Y. et al.
    • Total Pages
      1595
    • Publisher
      Academic Press
    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u,ac.jp/~bioact/matsu/index.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~bioact/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~bioact/

    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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