Evolutional study between speciation and venom gland isozymes of Protobothrops genus snakes inhabit the southwestern islands of Japan.

• Project/Area Number: 18687016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Sojo University
• Principal Investigator: CHIJIWA Takahito Sojo University, 生物生命学部, 准教授 (30331060)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2006: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
• Keywords: ハブ(Protobothrops flavoviridis) / ホスホリパーゼA2(PLA2) / アイソザイム / 加速進化 / 生物多様性 / 環境適応 / 南西諸島 / ホスホリパーゼA_2 / ホスホリパーゼA_2(PLA_2)

Research Abstract

日本南西諸島に棲息するハブ属ヘビの中でも、島同士が明らかに隔離しており、背中の模様や体長などが島特異的なハブ属ヘビ(トカラハブ、サキシマハブ、イヘヤジマハブ)を対象として、それぞれの粗毒に含まれる毒腺ホスホリパーゼA2(PLA2)アイソザイムの組成やアミノ酸配列を明らかにした。島特異的なこれらの分子進化と、ミトコンドリアDNAの塩基配列を基にした各ヘビの分岐関係とを照らし合わせ、毒腺PLA2は餌などの棲息環境因子に適応して進化してきたことを見出した。

Research Products

• [Journal Article] Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia) (2008)
  • Author(s): 松原和純、山田和彦、梅本周、土屋公幸、池田直樹、西田千鶴子、千々岩崇仁、森脇和郎、松田洋一
  • Journal Title: Chromosome Res. 16 Pages: 1013-1026
  • Peer Reviewed
• [Journal Article] Identification of the B subtype of γ-phospholipase A2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A2 inhibitors (2008)
  • Author(s): 宗修平、千々岩崇仁、 池田直樹、信久幾夫、 上田直子、服部正策、大野素徳
  • Journal Title: Journal of Molecular Evolution 66 Pages: 198-307
  • Peer Reviewed
• [Journal Article] A novel recombinant system for functional expression of myonecrotic snake phospholipase A2 in Escherichia coli using a new fusion affinity tag (2008)
  • Author(s): 瀬戸美苗、小川智久、児玉教佑、金山徳孝、酒井康夫、千々岩崇仁、大野素徳
  • Journal Title: Protein Expression and Purification 58 Pages: 194-202
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apoidemes peninsulae Muridae. Rodentia) (2008)
  • Author(s): Matsubara K. Yamada K. Umemoto S. Tsuchiya K. Ikeda N. Nishida C. Chijiwa T. Moriwaki K. Matsuda Y.
  • Journal Title: Chromosome Res 16 Pages: 1013-1026
  • Peer Reviewed
• [Journal Article] A novel recombinant system for functional expression of myonecrotic snake phosphoUpase A2 in Escherichia coli using a new fusion affinity tag (2008)
  • Author(s): Seto M, Ogawa T, Kodama K, Kanayama Y, Saliai Y, Chiiiwa T. Ohno M
  • Journal Title: Protein Expression and Purification 58 Pages: 194-202
  • Peer Reviewed
• [Journal Article] Identification of the B siibtype of vphospholipase A2 inhibitor from Protobothrops Qavovindis serum and molecular evolution of snake serum DhosDholiDase A2 inhibitors (2008)
  • Author(s): So S, Chijiwa T, Ikeda N, Nobuhisa l, 0 da-Ueda N Hattori S. Ohno M
  • Journal Title: Journal of Molecular Evolution (In press)
  • Peer Reviewed
• [Journal Article] Discovery of novel [Arg49]phospholipase A2 isozymes from Protobothrops elegans venom and regional evolution of Crotalinae snake venom phospholipase A2 isozymes in the southwestern islands of Japan and Taiwan (2006)
  • Author(s): 千々岩崇仁、徳永絵美、池田良、寺田考紀、小川智久、上田直子、服部正策、大野素徳
  • Journal Title: Toxicon 48 Pages: 672-682
  • Peer Reviewed
• [Journal Article] Discovery of novel [Arg^<49>]phospholipase A_2 isozymes from Protobothrops elegans venom and regional evolution of Crotalinae snake venom phospholipase A_2 isozymes in the southwestern islands of Japan and Taiwan (2006)
  • Author(s): Takahito Chijiwa, Emi Tokunaga, Ryo Ikeda, Koki Terada, Tomohisa Ogawa, Naoko Oda-Ueda, Shosaku Hattori, Masatoshi Nozaki, Motomri Ohno
  • Journal Title: Toxicon 48 Pages: 672-682
• [Presentation] 伊平屋島ハブ毒腺ホスホリパーゼA2の網羅的解析 (2008)
  • Author(s): 友田貫太郎
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-13
• [Presentation] 奄美大島ハブ毒腺Phospholipase A2ゲノム領域の解読 (2008)
  • Author(s): 原弘昭
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 日本の南西諸島3島(奄美大島、徳之島、沖縄)のハブ(Protobothrops flavoviridis)毒成分の比較プロテオミクス (2008)
  • Author(s): 高崎伸也
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 日本の地域毎のマムシ毒の比較プロテオミクス (2008)
  • Author(s): 村上達夫
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Retrotransposons had been possibly responsible for multiplication of venom phospholipase A2 isozyme genes of Protobothrops flavoviridis (2008)
  • Author(s): 千々岩崇仁
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 日本の南西諸島3島(奄美大島、徳之島、沖縄)のハブ(Protobothrops flavoviridis)毒成分の比較ブロテオミクス (2008)
  • Author(s): 高崎伸也
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] ハブ(P. flavoviridis)血清由来γホスホリパーゼA2インヒビターの遺伝子構造と分子進化 (2008)
  • Author(s): 千々岩崇仁
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] ハブ(P. flavoviridis)血清由来γボスホリパーゼA2インヒビターの遺伝子構造と分子進化 (2008)
  • Author(s): 千々岩崇仁
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] マムシ亜科毒ヘビ膵臓ホスホリパーゼA2遺伝子の比較解析 (2008)
  • Author(s): 入江紗己子
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ミトコンドリアDNAの塩基配列を基にした奄美大島ホンハブの系統解析 (2008)
  • Author(s): 山村岳司
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] ハブ毒Phospholipase A2遺伝子の多重化に関わるレトロトランスポジション (2008)
  • Author(s): 千々岩崇仁
  • Organizer: 「生体分子による生物間攻撃と防御」シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] ハブ毒腺Phospholipase A2遺伝子の多重化に関わるレトロトランスポジション (2008)
  • Author(s): 千々岩崇仁
  • Organizer: 日本遺伝学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-05
• [Presentation] Identification of B subtype of γ-phospholipase A2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A2 inhibitors (2008)
  • Author(s): 宗修平
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-05
• [Presentation] ハブ毒中の単独で作用する補体活性化因子の構造と機能について (2008)
  • Author(s): 上田直子
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-04
• [Presentation] 奄美大島ハブ毒中の[Lys49]PLA2の細胞死誘導機構 (2008)
  • Author(s): 村上達夫
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-05-19
• [Presentation] 徳之島ハブ毒中の補体活性化因子の精製と諸性質 (2008)
  • Author(s): 鈴木美緒
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-05-19
• [Presentation] 伊平屋島ハブ毒腺phospholipase A2(PLA2)の網羅的解析 (2008)
  • Author(s): 友田貫太郎
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-05-19
• [Presentation] ミトコンドリアDNAによる奄美大島ホンハブの系統解析 (2007)
  • Author(s): 山村岳司
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 本島系ハブから見るハブ毒phospholipase A2(PLA2)の進化解析 (2007)
  • Author(s): 友田貫太郎
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 奄美大島ハブ毒特異的な新規な[Lys49]ホスホリパーゼA2 (2007)
  • Author(s): 村上達夫
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 本島系ハブから見るハブ毒phospholipase A2 (PLA2)の進化解析 (2007)
  • Author(s): 友田貫太郎
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] ハブ毒中の新規なC3/C5コンベルターゼの精製と諸性質 (2007)
  • Author(s): 鈴木美緒
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ハブ毒中の新規なC3/C5コンベルターゼの精製と書性質 (2007)
  • Author(s): 鈴木美緒
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ハブ毒phospolipase A2をコードするゲノム領域の解析 (2007)
  • Author(s): 池田直樹
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Retrotransposons had been possibly responsible for multiplication of venom phospholipase A2 isozyme genes of Protobothrops flavoviridis (2007)
  • Author(s): 千々岩崇仁
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] ハブ毒pospolipase A2をコードするゲノム領域の解析 (2007)
  • Author(s): 池田直樹
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Identification of B subtype of y-phospholipase A2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A2 inhibitors (2007)
  • Author(s): 宗 修平
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-09-05
• [Presentation] 伊平屋島ハブ毒腺phospholipase A_2(PLA_2)の網羅的解析 (2007)
  • Author(s): 友田 貫太郎
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2007-05-19
• [Presentation] 徳之島ハブ毒中の補体活性化因子の精製と諸性質 (2007)
  • Author(s): 鈴木 美緒
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2007-05-19
• [Presentation] 奄美大島ハブ毒中の[Lys^<49>]PLA_2の細胞死誘導機構 (2007)
  • Author(s): 村上 達夫
  • Organizer: 平成19年度日本生化学会九州支部会
  • Place of Presentation: 宮崎
  • Year and Date: 2007-05-19