Molecular mechanisms of fungal energy conservation

• Project/Area Number: 18688003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: University of Tsukuba
• Principal Investigator: TAKAYA Naoki University of Tsukuba, 大学院・生命環境科学研究科, 准教授 (50282322)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2006: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
• Keywords: エネルギー代謝 / 異化 / 発酵 / ミトコンドリア / オミクス / 真菌 / 嫌気呼吸 / 分子進化 / カビ

Research Abstract

カビや酵母などの真菌は、醸造・発酵産業に利用され我々の生活に利益をもたらすとともに、動植物の病原菌として厄介な存在でもある。本研究では、真菌の生命の維持に必要なエネルギー代謝に注目し、これを解明することによって、真菌の生育(繁殖)の制御に関する知見を得ることを試みている。また、得られた知見を他の生物のそれと比較・検証することにより、真菌のエネルギー獲得機構のなりたちと分子進化を考察することを目指した。

Research Products

• [Journal Article] Mechanism of de novo branched-chain amino acid synthesis as alternative electron sink in hypoxic Aspergillus nidulans cells. (2010)
  • Author(s): 志水元亨、藤井達也、枡尾俊介、高谷直樹
  • Journal Title: Appl. Environ. Microbiol. 76 Pages: 1507-1515
  • Peer Reviewed
• [Journal Article] The glutathione system of Aspergillus nidulans involves a fungal specific glutathione S-transferase. (2009)
  • Author(s): 佐藤育男、志水元亨、星野貴行、高谷直樹
  • Journal Title: J. Biol. Chem. 284 Pages: 8042-8053
  • Peer Reviewed
• [Journal Article] Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions (2009)
  • Author(s): 志水元亨、藤井達也、枡尾俊介、藤田健作、高谷直樹
  • Journal Title: Proteomics 9 Pages: 7-19
  • Peer Reviewed
• [Journal Article] Response to hypoxia, reduction of electron acceptors, and subsequent survival by filamentous fungi (2009)
  • Author(s): 高谷直樹
  • Journal Title: Biosci. Biotech. Biochem. 73 Pages: 1-8
  • NAID: 10027535611
  • Peer Reviewed
• [Journal Article] Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions (2009)
  • Author(s): Shimizu, M., Fujii, T., Masuo, S.Fujita, K., Takaya, N.
  • Journal Title: Proteomics 9 Pages: 7-19
  • Peer Reviewed
• [Journal Article] The glutathione system of Aspergillus nidulans involves a fungal specific glutathione-S-transferase (2009)
  • Author(s): Sato, I., Shimizu, M., Hoshino, T., Takaya, N.
  • Journal Title: J.Biol.Chem. (印刷中)
  • Peer Reviewed
• [Journal Article] Novel dehydrogenase catalyzes oxidative hydrolysis of carbon- nitrogen double bonds for hydrazone degradation. (2008)
  • Author(s): 伊藤英臣、鈴田哲也、星野貴行、高谷直樹
  • Journal Title: J. Biol. Chem. 283 Pages: 5790-5800
  • Peer Reviewed
• [Journal Article] Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase. (2008)
  • Author(s): 藤井達也、高谷直樹
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 412-420
  • NAID: 10027524224
  • Peer Reviewed
• [Journal Article] Denitrification by the fungus Fusarium oxysporum involves NHDH-nitrate reductase (2008)
  • Author(s): Fujii, T., Takaya, N.
  • Journal Title: Biosci Biotechnol Biochem. 72 Pages: 412-420
  • Peer Reviewed
• [Journal Article] Denitrification by the fungus Fusarium oxyspoyum involves NADH-nutrate reductase (2008)
  • Author(s): Fujii, T., Takaya, N.
  • Journal Title: Biosci.Biotech.Biochem 72・5 Pages: 412-420
  • Peer Reviewed
• [Journal Article] Novel dehydrogenase catalyzes oxidative hydrolsys of carbonnitrogen double bonds for gydrozone degradation (2008)
  • Author(s): Itoh, H., Suzuta, T., Hoshino, T., and Takaya, N.
  • Journal Title: J.Biol.Chem 283・9 Pages: 5790-5800
  • Peer Reviewed
• [Journal Article] Anaerobic elemental sulfur reduction by fungus Fusarium oxysporum. (2007)
  • Author(s): 安部剛史、星野貴行、高谷直樹
  • Journal Title: Biosci. Biotechnol. Biochem. 71 Pages: 2402-2407
  • NAID: 10027519599
  • Peer Reviewed
• [Journal Article] 息づまる闘い-カビの生き残り戦略? (2007)
  • Author(s): 高谷直樹、志水元亨
  • Journal Title: 生物工学会誌 85 Pages: 416-416
  • NAID: 110006402935
  • Peer Reviewed
• [Journal Article] Anaerobic elemental sulfur reduction by fungus Fusarium oxysporum (2007)
  • Author(s): Abe, T., Hoshino, T., Nakamura, A., and Takaya, N.
  • Journal Title: Biosci.Biotech.Biochem 71・4 Pages: 2402-2407
  • NAID: 10027519599
  • Peer Reviewed
• [Journal Article] Novel family of cholesterol esterases produced by actinomycetes bacteria. (2007)
  • Author(s): Xiang, H., Masuo, S., Hoshino, T., Takaya, N.
  • Journal Title: Biochim Biophys Acta. 1774 Pages: 112-120
• [Journal Article] カビによる新規な異化代謝の機能解明とその利用 (2007)
  • Author(s): 安部剛史, 高谷直樹
  • Journal Title: 環境バイオテクノロジー学会誌 6(印刷中)
• [Presentation] Microbial Interactions Leading to Novel Biological Functions (2010)
  • Author(s): Sato, I., Shimiatani, K., Shimizu, M., Takaya, N.
  • Organizer: Roles of Aspergillus nidulans proteins in thioredoxin reductase superfamily
  • Place of Presentation: Tsukuba
  • Year and Date: 2010-01-08
• [Presentation] Nudix hydrolaseの低酸素条件下での役割 (2010)
  • Author(s): 志水元亨、桝尾俊介、藤田智也、高谷直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] Aspergillus nidulansの元素状硫黄還元反応に関わる酵素の機能解析 (2010)
  • Author(s): 島谷佳奈果、佐藤育男、高谷直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] Aspergillus nidulansのメナジオンストレス下におけるNmrAの機能 (2010)
  • Author(s): 桝尾俊介、志水元亨、高谷直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] Pseudomonas aeruginosa由来のヒドラゾン脱水素酵素の性質 (2010)
  • Author(s): 高谷直樹、伊藤英臣、佐々木康幸、池田智、矢嶋俊介、谷山浩將
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
• [Presentation] Microorganisms and Sociality: Intricate Multi-Level Interactions (2009)
  • Author(s): Masuo, S., Fujii, T., Takaya, N.
  • Organizer: Hypoxic nitrate reduction and its regulation by the fungus Aspergillus nidulans
  • Place of Presentation: Tsukuba
  • Year and Date: 2009-11-24
• [Presentation] 低酸素条件下におけるNudix hydrolaseの役割 (2009)
  • Author(s): 志水元亨, 桝尾俊介, 藤田智也、藤井達也、高谷直樹
  • Organizer: 第9回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京
• [Presentation] (2009)
  • Author(s): Shimizu, M., Masuo, S. Takaya, N.
  • Organizer: Xth International Conference of Fungal Biology
  • Place of Presentation: Ensenada, Mexico
• [Presentation] (2009)
  • Author(s): Shimiatani, K., Sato, I. Takaya, N
  • Organizer: Xth International Conference of Fungal Biology
  • Place of Presentation: Ensenada, Mexico
• [Presentation] 分岐アミノ酸生合成の低酸素条件下での役割 (2009)
  • Author(s): 志水元亨, 藤井達也, 桝尾俊介, 高谷直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] Aspergillus nidulansの亜硝酸耐性に関わる遺伝子の単離と解析 (2009)
  • Author(s): 鳴神寿昭, 桝尾俊介, 星野貴行, 高谷直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] Aspergillus nidulansのグルタチオン還元酵素と酸化ストレス応答 (2009)
  • Author(s): 佐藤育男, 藤井達也, 星野貴行, 高谷直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] 糸状菌による元素状硫黄還元反応におけるチオレドキシン還元酵素の役割 (2009)
  • Author(s): 島谷佳奈果, 佐藤育男, 志水元亨, 藤田健作, 高谷直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] Aspergillus nidulansの硝酸還元酵素遺伝子(niaD)の低酸素条件下での転写制御 (2008)
  • Author(s): 桝尾俊介, 藤井達也, 高谷直樹
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 金沢
• [Presentation] 低酸素条件下におけるチアミン生合成の役割 (2008)
  • Author(s): 志水元亨, 藤井達也, 桝尾俊介, 高谷直樹
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 金沢