Improvement of CO2-fixing enzyme, RuBisCO, based on molecular evolution to enhance photosynthesis of plant.
Project/Area Number |
18688021
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Applied molecular and cellular biology
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
ASHIDA Hiroki Nara Institute of Science and Technology, バイオサイエンス研究科, 助教授 (50362851)
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Project Period (FY) |
2006 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2006: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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Keywords | 植物 / 光合成 / CO2固定 / ルビスコ / 分子進化 / 紅藻 / 葉緑体形質転換 / ルビスコ祖先蛋白質 / Galdieria |
Research Abstract |
ルビスコ祖先タンパク質とルビスコの比較解析から、両酵素で共通な触媒残基を明らかにした。これを基に祖先タンパク質にルビスコ触媒必須残基を導入し、試験管内でルビスコへの分子進化に成功した。紅藻ルビスコが持つ高CO_2識別残基をラン藻ルビスコに導入し、CO_2識別能を20%高めることに成功した。さらに、この残基をタバコルビスコに導入したタバコ形質転換体を作出した。紅藻ルビスコ遺伝子導入タバコの解析から、高機能外来性ルビスコの葉緑体機能発現の問題点を明らかにし、その解決策を導き出した。
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] A New Rubisco-like Protein Coexists with a Photosynthetic Rubisco in the Planktonic Cyanobacteria Microcystis2006
Author(s)
Alyssa Carre-Mlouka, Annick Mejean, Philippe Quillardet, Hiroki Ashida, Yohtaro Saito, Akiho Yokota, Isabelle Callebaut, Agnieszka Sekowska, Elke Dittmann, Christiane Bouchier, and Nicole Tandeau de Marsac
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Journal Title
J. Biol. Chem. 281
Pages: 24462-24471
Related Report
Peer Reviewed
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[Journal Article] A new Rubisco-like protein coexists with a photosynthetic Rubisco in the planktonic cyanobacteria Microcystis2006
Author(s)
Carre-Mlouka, A., Mejean, A., Quillardet, P., Ashida H., Saito, Y., Yokota, A., Callebaut, I., Sekowska, A., Dittmann, E., Bouchier, C., de Marsac, N.T.
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Journal Title
Journal of Biological Chemistry 281・34
Pages: 24462-24471
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[Presentation] イネ由来RuBisCOと紅藻由来RuBisCOの硫酸イオン複合体に見られた立体構造の違い2007
Author(s)
小村泰浩, 松村浩由, 蘆田弘樹, 石田宏幸, 上野剛, 溝端栄一, 井上豪, 牧野周, 横田明穂, 前忠彦, 甲斐泰,
Organizer
日本結晶学会2007年度年会および総会
Place of Presentation
東京
Related Report
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[Presentation] STRUCTURAL DIFFERENCE BETWEEN RICE AND RED ALGA RUBISCO COMPLEXED WITH SULFATE2007
Author(s)
Yasuhiro Komura, Hiroyoshi Matsumura, Hiroyuki Ishida, Hiroki Ashida, Eiichi Mizohata, Tsuyoshi Inoue, Amane Makino, Tadahiko Mae, Akiho Yokota, Yasushi Kai
Organizer
The 8th Conference of the Asian Crystallographic Association, AsCA' 07 第8回アジア結晶学会連合会議
Place of Presentation
台北
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[Presentation] STRUCTURAL DIFFERENCE BETWEEN RICE AND ALGA RUBISCO COMPLEXED WITH SULFATE.2007
Author(s)
Yasuhiro Komura, Hiroyoshi Matsumura, Hiroyuki Ishida, Hiroki Ashida, Eiichi Mizohata, Tsuyoshi Inoue, Amane Makino, Tadahiko Mae, Akiho Yokota, Yasushi Kai
Organizer
The 8th Conference of the Asian Crystallographic Association, AsCA'07
Place of Presentation
台北
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[Presentation] RuBisCO-like proteins2007
Author(s)
Hiroki ASHIDA and Akiho YOKOTA
Organizer
Research Frontiers with Rubisco - the "Elixir of Life" in the Biosphere. 14^th International Congress of Photosynthesis satellite Meeting, guest speaker
Place of Presentation
Harpenden, UK
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[Presentation] Molecular evolution of RuBisCO-like proteins
Author(s)
Hiroki Ashida, Tomoko Sakiyama, Yohtaro Saito, Nicole Tandeau de Marsac and Akiho Yokota
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto, Japan
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