Identification of novel intrinsic anti-lentiviral defense mechanisms

• Project/Area Number: 18689014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: 駒野 淳 National Institute of Infectious Diseases (60356251)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000); Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2007: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2006: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: ウイルス / 生体分子 / 感染症

Research Abstract

我々はHIV-1複製を制御する宿主因子としてHEXIM1,Cyclin K,HSP40B6/DNAJB6,Arp2/3 complex,CD63,Brd4などを同定した。HEXIM1、Cyclin K、Brd4 C末端ドメインはTat/LTR転写を特異的に阻害する事により抗HIV-1複製作用を示し、HSP40B6/DNAJB6は新たなメカニズムでウイルス転写を抑制することによりHIV-1複製を抑止すること、CD63はウイルスレセプターCXCR4の細胞表面発現レベルを低下させることによりHIV-1侵入を阻害すること、Arp2/3複合体がGagと直接相互作用することによりウイルスが細胞に侵入する過程を制御することを証明した。我々は世界でも例をみないユニークなT細胞を使用したHIV-1抵抗性因子をcDNAからスクリーニングする実験系を確立し、これを基幹技術としてさらに数多くのHIV-1複製制御因子を同定し、細胞がもつ内在性抗レトロウイルス因子(intrinsic immunity)に関する研究領域における著しい学術的発展に繋げることに成功した。本研究は霊長類レンチウイルス複製の分子メカニズムを明らかにし、エイズウイルスをはじめとする病原性レトロウイルスの治療法を開発するために大きく貢献し、今後も更なる発展が期待される。

Research Products

• [Journal Article] Derivatives of 5-nitro-furan-2-carboxylic acid carbamoylmethyl ester inhibit RNase Hactivity associated with HIV-1 reverse transcriptase (2009)
  • Author(s): Fuji H, Urano E, Futahashi Y, Hamatake M, Tatsumi J, Hoshino T, Morikawa Y, Yamamoto N, Komano J.
  • Journal Title: J Med Chem. (in press)
• [Journal Article] Ligand-independent higher-order multimerization of CXCR4, a G-protein-coupled chemokine receptor involved in the targeted metastasis (2009)
  • Author(s): Hamatake M, Aoki T, Futahashi Y, Urano E, Yamamoto N, Komano J.
  • Journal Title: Cancer Sci 100(1) Pages: 95-102
• [Journal Article] Derivatives of 5-nitro-furan-2-carboxylic acid carbamoylmethyl ester inhibit RNase H activity associated with HIV-1 reverse transcriptase. (2009)
  • Author(s): Fuji H, Urano E, Futahashi Y, Hamatake M, Tatsumi J, Hoshino T, Morikawa Y, Yamamoto N, Komano J.
  • Journal Title: J Med Chem. 52(5) Pages: 1380-1387
  • Peer Reviewed
• [Journal Article] Ligand-independent higher-order multimerization of CXCR4, a G-protein-coupled chemokine receptor involved in the targeted metastasis. (2009)
  • Author(s): Hamatake M, Aoki T, Futahashi Y, Urano E, Yamamoto N, Komano J.
  • Journal Title: Cancer Sci. Jan;100(1) Pages: 95-102
  • Peer Reviewed
• [Journal Article] Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cDNA library screening in MT-4 cells (2008)
  • Author(s): Urano E, Kariya Y, Futahashi Y, Ichikawa R, Hamatake M, Fukazawa H, Morikawa Y, Yoshida T, Koyanagi Y, Yamamoto N, Komano J.
  • Journal Title: FEBS Let. 582(29) Pages: 4053-8
• [Journal Article] Substitution of the myristoylation signal of human immunodeficiency vius type 1 Pr55Gag with the phospholipase C delta 1 pleckstrin homology domain results in infectious pseudovirion production (2008)
  • Author(s): Urano E, Aoki T, Futahashi Y, Murakami T, Morikawa Y, Yamamoto N, Komano J.
  • Journal Title: J Gen Virol. 89 Pages: 3144-9
• [Journal Article] Cyclin K/CPR4 inhibits primate lentiviral replication by inactivating Tat/positive transcription elongation factor b-dependent long terminal repeat transcription (2008)
  • Author(s): Urano E, Shimizu S, Futahashi Y, Hamatake M, Morikawa Y, Takahashi N, Fukazawa H, Yamamoto N, Komano J.
  • Journal Title: AIDS 22(9) Pages: 1081-3
• [Journal Article] A CD63 mutant inhibits T-cell tropic human immunodeficiency virus type 1 entry by disrupting CXCR4 trafficking to the plasma membrane (2008)
  • Author(s): Yoshida T, Kawano Y, Sato K, Ando Y, Aoki J, Miura Y, Komano J, Tanaka Y, Koyanagi Y.
  • Journal Title: Traffic 9(4) Pages: 540-58
• [Journal Article] SOCS1 is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag (2008)
  • Author(s): Ryo A, Tsurutani N, Ohba K, Kimura R, Komano J, 他15名
  • Journal Title: Proc Natl Acad Sci USA 105(1) Pages: 294-9
• [Journal Article] Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cDNA library screening in MT-4 cells. (2008)
  • Author(s): Urano E, Kariya Y, Futahashi Y, Ichikawa R, Hamatake M, Fukazawa H, Morikawa Y, Yoshida T, Koyanagi Y, Yamamoto N, Komano J.
  • Journal Title: FEBS Let. Dec 10; 5 82(29) Pages: 4053-8
  • Peer Reviewed
• [Journal Article] Substitution of the myristoylation signal of human immunodeficiency vius type 1 Pr55Gag with the phospholipase C delta 1 pleckstrin homology domain results in infectious pseudovirion production. (2008)
  • Author(s): Urano E, Aoki T, Futahashi Y, Murakami T, Morikawa Y, Yamamoto N, Komano J.
  • Journal Title: J Gen Virol. Dec; 89(Pt 12) Pages: 3144-9
  • Peer Reviewed
• [Journal Article] Cyclin K/CPR4 inhibits primate lentiviral replication by inactivating Tat/positive transcription elongation factor b-dependent long terminal repeat transcription. (2008)
  • Author(s): Urano E, Shimizu S, Futahashi Y, Hamatake M, Morikawa Y, Takahashi N, Fukazawa H, Yamamoto N, Komano J.
  • Journal Title: AIDS. May 31;22(9) Pages: 1081-3
  • Peer Reviewed
• [Journal Article] A CD63 mutant inhibits T-cell tropic human immunodeficiency virus type 1 entry by disrupting CXCR4 trafficking to the plasma membrane. (2008)
  • Author(s): Yoshida T, Kawano Y, Sato K, Ando Y, Aoki J, Miura Y, Komano J. Tanaka Y, Koyanagi Y.
  • Journal Title: Traffic. Apr;9(4) Pages: 540-58
  • Peer Reviewed
• [Journal Article] Cyclin K/CPR4 inhibits primate lentiviral replication by inactivating Tat/P-TEFb-dependent LTR transcription (2008)
  • Author(s): Emiko Urano, Saki Shimizu, Yuko Futahashi, Makiko Hamatake, Yuko Morikawa, Naoko Takahashi, Hidesuke Fukazawa, Naoki Yamamoto, *.Jun Komano.
  • Journal Title: AIDS 22 Pages: 1081-1089
  • Peer Reviewed
• [Journal Article] SOCSl is aninducible host factor during HN-1 infection and regulates the intracellular trafficking and stability of HN-1 Gag (2008)
  • Author(s): Akihide Ryo, Naomi Tsurutani, Kenji Ohba, Ryuichiro Kimura, Jun Komano, Mayuko Nishi, Hiromi Soedal, Shinichiro Hattori, Kilian Perrem, Mikio Yamamoto, Joh Chiba, Jun-ichi Mimaya, Kazuhisa Yoshimura, Shuzo Matsushita, Mitsuo Honda, Akihiko Yoshimura, Ichiro Aoki, Yuko Morikawa and Naoki Yamamoto
  • Journal Title: Proc Natl Acad SciU S A 105(1) Pages: 297-299
  • Peer Reviewed
• [Journal Article] CD63 and its mutants disrupt CXCR4 trafficking to the plasma membrane and inhibit T-cell tropic HIV-1 entry (2008)
  • Author(s): Takeshi Yoshida, Yuji Kawano, Kei Sato, Yoshiharu Miura, Yoshinori Ando, Jun Aoki, Jun Komano, Yuetsu Tanaka, Yoshio Koyanagi.
  • Journal Title: Traffic 9(4) Pages: 540-558
  • Peer Reviewed
• [Journal Article] Inhibiting lentiviral replication by HEXIM1, a cellular negative regulator of the CDK9/cyclin T complex (2007)
  • Author(s): Shimizu S, Urano E, Futahashi Y, Miyauchi K, Isogai M, Matsuda Z, Nohtomi K, Onogi T, Takebe Y, Yamamoto N, Komano J.
  • Journal Title: AIDS 21(5) Pages: 575-82
• [Journal Article] Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes (2007)
  • Author(s): Kameoka M, Kitagawa Y, Utachee P, Jinnopat P, Dhepakson P, Isarangkura-na-ayuthaya P, Tokunaga K, Sato H, Komano J, Yamamoto N, Oguchi S, Natori Y, Ikuta K.
  • Journal Title: Biochem Biophys Res Commun. 359(3) Pages: 29-34
• [Journal Article] Separate elements are required for ligand-dependent and-independent internalization of metastatic potentiator CXCR4 (2007)
  • Author(s): Futahashi Y, Komano J, Urano E, Aoki T, Hamatake M, Miyauchi K, Yoshida T, Koyanagi Y, Matsuda Z, Yamamoto N.
  • Journal Title: Cancer Sci 98(3) Pages: 373-9
• [Journal Article] In vitro translation to study HIV protease activity (2007)
  • Author(s): Matsuda Z, Iga M, Miyauchi K, Komano J, Morishita K, Okayama A, Tsubouchi H.
  • Journal Title: Methods Mol Biol 375 Pages: 135-49
• [Journal Article] In vitro translation to study HIV protease activity (2007)
  • Author(s): Matsuda Z, Iga M, Miyauchi K, Komano J, Morishita K, Okayama A, Tsubouchi H.
  • Journal Title: Methods Mol Bio 375 Pages: 135-149
  • Peer Reviewed
• [Journal Article] Identification of the suppressive factors for human immunodeficiencyvirustype-1 replication using the siRNA mini-library directed against host cellular genes (2007)
  • Author(s): Kameoka M, Kitagawa Y, Utachee P, Jinnopat P, Dhepakson P, Isarangkura-na-ayuthaya P, Tokunaga K, Sato H, Komano J, Yamamoto N, Oguchi S, Natori Y, Ikuta K.
  • Journal Title: Biochem Biophys Res Commun 359(3) Pages: 729-734
  • Peer Reviewed
• [Journal Article] Inhibiting lentiviral replication by HEXIM1,a cellular negative regulatoro the CDK9/cyclin T complex (2007)
  • Author(s): Shimizu S, Urano E, Futahashi Y, Miyauchi K, Isogai M, Matsuda Z, Nohtomi K, Onogi T, Takebe Y, Yamamoto N, Komano J.
  • Journal Title: AIDS 21(5) Pages: 575-582
  • Peer Reviewed
• [Journal Article] ARVのUniversal Access時代を迎えて;エイス研究センターの国際研修活動について (2007)
  • Author(s): 村上 努、駒野 淳
  • Journal Title: 病原微生物検出情報lnfectious Agents Surveillance Report(IASR) 28(6) Pages: 164-166
• [Journal Article] Regulation of Gag trafficiking and functions(Review) (2007)
  • Author(s): Murakami T, Gottlinger H, Morikawa Y, Komano J, Ryo A, Sato H.
  • Journal Title: The Journal of AIDS Research 9(2) Pages: 102-107
• [Journal Article] Inhibiting lentiviral replication by HEX1M1, a cellular inhibitor of cdk9/cyclinT complex. (2007)
  • Author(s): Shimizu S, Urano E, Futahashi Y, Miyjauchi K, Isogai M, Matsuda Z, Nohtomi K, Onogi T, Takebe Y, Yamamoto N, Komano J
  • Journal Title: AIDS 21(5) Pages: 575-582
• [Journal Article] Separate elements are required for ligand-dependent and -independent internalization of metastatic potentiator CXCR4. (2007)
  • Author(s): Futahashi Y, *Komano J, Urano E, Aoki T, Hamatake M, Miyauchi K, Yoshida T, Koyanagi Y, Matsuda Z, Yamamoto N
  • Journal Title: Cancer Sci 98(3) Pages: 373-379
• [Journal Article] Mutations of conserved glycine residues within the membrane-spanning domain of human immunodeficiency virus type 1 gp41 can inhibit membrane fusion and incorporation of Env onto virions (2006)
  • Author(s): Miyauchi K, Curran R, Matthews E, Komano J, Hoshino T, Engelman DM, Matsuda Z.
  • Journal Title: Jpn J Infect Dis. 59(2) Pages: 77-84
• [Journal Article] Rapid propagation of low-fitness drug-resistant mutants of human immunodeficiency virus type 1 by a streptococcal metabolite sparsomycin (2006)
  • Author(s): Miyauchi K, Komano J, Myint L, Futahashi Y, Urano E, Matsuda Z, Chiba T, Miura H, Sugiura W, Yamamoto N.
  • Journal Title: Antivir Chem Chemother. 17(4) Pages: 167-74
• [Journal Article] Mutations of conserved glycine residues within the membrane-spanning domain of human immunodeficiency virus type 1 gp41 can inhibit membrane fusion and incorporation of Env onto virions. (2006)
  • Author(s): Miyayuchi K, Curran R, Matthews E, Komano J, Hoshino T, Engelman DM, *Matsuda Z
  • Journal Title: Jpn J Infect Dis. 59(2) Pages: 77-84
• [Journal Article] Rapid propagation of low-fitness drug resistant mutants of human immunodeficiency virus type 1 by a streptococcal metabolite sparsomycin. (2006)
  • Author(s): Miyauchi K, *Komano J, Myint L, Futahashi Y, Urano E, Matsuda Z, Chiba T, Miura H, Sugiura W, Yamamoto N
  • Journal Title: Antivir Chem Chemother. 17(4) Pages: 167-174
• [Journal Article] Role of the specific amino acid sequence of the membrane-spanning domain of human immunodeficiency virus type 1 in membrane fusion (2005)
  • Author(s): Miyauchi K, Komano J, Yokomaku Y, Sugiura W, Yamamoto N, Matsuda Z.
  • Journal Title: Journal of Virology 79(8) Pages: 4720-9
• [Presentation] BiFC/BRETによる癌転移増強分子CXCR4のLigand非依存的な多量体形成の解析. (2008)
  • Author(s): 駒野淳, 濱武牧子, 青木徹, 浦野恵美子, 二橋悠子, 山本直樹.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] A CD63 mutant inhibits CXCR4 trafficking to the plasma membrane and blocks X4 HIV-1 entry (2008)
  • Author(s): Takeshi Yoshida, Yuji Kawano, Yoshinori Ando, Kei Sato, Jun Komano, Yuetsu Tanaka, Yoshio Koyanagi
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Identification of the carboxy-terminal domain of bromodomain containing 4 as a specific silencer of HIV-1 replication (2008)
  • Author(s): Emiko Urano, Yuki Kariya, Yuko Futahashi, Makiko Hamatake, Yuko Morikawa, Takeshi Yoshida, Yoshio Koyanagi, Naoki Yamamoto, Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Functional substitution of the myristoylation signal of HIV-1 Gag with phospholipase C delta 1 pleckstrin homology domain (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Kazuo Terashima, Hirokazu Tamamura, Tsutom Murakami, Yuko Morikawa, Naoki Yamamoto, Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Detection of ligand-independent higher-order oligomerization state of a G-protein-coupled receptor CXCR4 by BiFC/BRET (2008)
  • Author(s): Makiko Hamatake, Yuko Futahashi, Toru Aoki, Naoki Yamamoto, Jun Komano
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] Substitution of the myristoylation signal of HIV-1 Pr55Gag with PLC delta lpleeckstrin homology domain results in fully infectious pseudovirion production (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Makiko Hamatake, Kazuo Terashima, Hirokazu Tamamura, Tsutomu Murakami, Yuko Morikawa, Naoki Yamamoto, Jun Komano
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] P-TEFb complex-interacting domain of Brd4 inhibits HIV-1 replication through restricting Tat-mediated enhancement of LTR promoter activity (2008)
  • Author(s): Emiko Urano, Yumi Kariya, Makiko Hamatake, Hidesuke Fukazawa, Yuko Morikawa, Yoshio Koyanagi, Naoki Yamamoto, Jun Komano.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] DNAJ/HSP40 Co-chaperone familyによるHIV-1複製抑制 (2008)
  • Author(s): 浦野恵美子, 奥長浩之、森川裕子、駒野連
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] T細胞におけるHIV-1抵抗性遺伝子のスクリーニング-Brd4C末端ドメインの同定とその機能解析 (2008)
  • Author(s): 駒野淳、浦野恵美子、刈屋祐美、二橋悠子、市川玲子、濱武牧子、深鰯秀輔、森川裕子、芳田剛、小柳義夫、山本直樹.
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] BiFc/BRETによる癌転移増強分子CXCR4のLigand非依存的な多量体形成の解析 (2008)
  • Author(s): 駒野淳、濱武牧子、青木徹、浦野恵美子、二橋悠子、山本直樹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] KRH-3955は経口投与可能な高活性抗X4HIV-1阻害剤である (2008)
  • Author(s): 村上努、大隈和、田中礼子、仲宗根正、濱武牧子、駒野淳、谷中幹郎、田中勇悦、山本直樹
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] HIV-1 Pre55Gagのミリストイル基非依存性ウイルス粒子産生と感染性 (2008)
  • Author(s): 青木徹、清水佐紀、浦野恵美子、濱武牧子、寺嶋一夫、玉村啓和、村上努、森川裕子、山本直樹、駒野淳
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] 宿主由来タンパクOX40L, OX40のHIV-1感染に与える影響 (2008)
  • Author(s): 高橋良明、村上努、駒野淳、古田篤司、田中礼子、山本直樹、田中勇悦
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] レンチウイルスバクターを用いた抗HIV因子のスクリーニングとその解析 (2008)
  • Author(s): 小林明子、芳田剛、駒野淳、小柳義夫
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] Detection of ligand-independent higher-order oligomerization state of a G-protein-coupled receptor CXCR4 by BiFC/BRET (2008)
  • Author(s): Makiko Hamatake, Yuko Futahashi, Toru Aoki, Naoki Yamamoto, Jun Komano
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] P-TEFb complex-interacting domain of Brd4 inhibits HIV-1 replication through restricting Tat-mediated enhancement of LTR promoter activity (2008)
  • Author(s): Emiko Urano, Yumi Kariya, Makiko Hamatake, Hidesuke Fukazawa, Yuko Morikawa, Yoshio Koyanagi, Naoki Yamamoto, Jun Komano
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] Substitution of the myristoylation signal of HIV-1 Pr55Gag with PLC delta 1 pleeckstrin homology domain results in fully infectious pseudovirion production (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Makiko Hamatake, Kazuo Terashima, Hirokazu Tamamura, Tsutomu Murakami, Yuko Morikawa, Naoki Yamamoto, Jun Komano
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] HIV-1のRNaseHを標的とした新規抗HIV薬の設計・評価・合成 (2008)
  • Author(s): 辰巳絢子、藤秀義、駒野淳、根矢三郎、星野忠次
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] A CD63 mutant inhibits CXCR4 trafficking to the plasma membrane and blocks X4 HIV-1 entry. (2008)
  • Author(s): Takeshi Yoshida, Yuji Kawano, Yoshinori Ando, Kei Sato, Jun Komano, Yuetsu Tanaka and Yoshio Koyanagi.
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY USA
• [Presentation] Identification of the carboxy-terminal domain of bromodomain containing 4 as a specific silencer of HIV-1 replication. (2008)
  • Author(s): Emiko Urano, Yuki Kariya, Yuko Futahashi, Makiko Hamatake, Yuko Morikawa, Takeshi Yoshida, Yoshio Koyanagi, Naoki Yamamoto, and Jun Komano.
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY USA
• [Presentation] Functional substitution of the myristoylation signal of HIV-1 Gag with phospholipase C delta 1 pleckstrin homology domain. (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Kazuo Terashima, Hirokazu Tamamura, Tsutom Murakami, Yuko Morikawa, Naoki Yamamoto and Jun Komano.
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY USA
• [Presentation] Detection of ligand-independent higher-order oligomerization state of a G-protein-coupled receptor CXCR4 by BiFC/BRET. (2008)
  • Author(s): Makiko Hamatake, Yuko Futahashi, Toru Aoki, Naoki Yamamoto, Jun Komano.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] Substitution of the myristoylation signal of HIV-1 Pr55Gag with PLC delta lpleeckstrin homology domain results in fully infectious pseudovirion production. (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Makiko Hamatake, Kazuo Terashima, Hirokazu T amamura, Tsutomu Murakami, Yuko Morikawa, Naoki Yamamoto, Jun Komano.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] P-TEFb complex-interacting domain of Brd4 inhibits HIV-1 replication through restricting Tat-mediated enhancement of LTR promoter activity. (2008)
  • Author(s): Emiko Urano, Yumi Kariya, Makiko Hamatake, Hidesuke Fukazawa, Yuko Morikawa, Yoshio Koyanagi, Naoki Yamamoto, Jun Komano.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity Awaji 2008
  • Place of Presentation: 兵庫
• [Presentation] DNA J/HSP40 Co-chaperone familyによるHIV-1複製抑制. (2008)
  • Author(s): 浦野恵美子, 奥長浩之, 森川裕子, 駒野淳.
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] T細胞におけるHIV-1抵抗性遺伝子のスクリーニング-Brd4C末端ドメインの同定とその機能解析. (2008)
  • Author(s): 駒野淳, 浦野恵美子, 刈屋祐美, 二橋悠子, 市川玲子, 濱武 牧子, 深觸秀輔, 森川裕子, 芳田剛, 小柳義夫, 山本直樹.
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] KRH-3955は経口投与可能な高活性抗X4HIV-1阻害剤である. (2008)
  • Author(s): 村上努, 大隈和, 田中礼子, 仲宗根正, 濱武牧子, 駒野淳, 谷中幹郎, 田中勇悦, 山本直樹.
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] HIV-1 Pre55Gagのミリストイル基非依存性ウイルス粒子産生と感染性. (2008)
  • Author(s): 青木徹, 清水佐紀, 浦野恵美子, 濱武牧子, 寺嶋一夫, 玉村啓和, 村上努, 森川裕子, 山本直樹, 駒野淳.
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] 宿主由来タンパクOX40L, OX40のHIV-1感染に与える影響. (2008)
  • Author(s): 高橋良明, 村上努, 駒野淳, 古田篤司, 田中礼子, 山本直樹, 田中勇悦
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] Inhibition of HIV-1 replicstion by co-chaperone DNA J/HSP40 protein family. (2008)
  • Author(s): 浦野恵美子, 奥長浩之, 森川裕子, 山本直樹, 駒野淳
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] レンチウイルスバクターを用いた抗HIV因子のスクリーニングとその解析. (2008)
  • Author(s): 小林明子, 芳田剛, 駒野淳, 小柳義夫
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
• [Presentation] Detection of ligand-independent higher-order oligomerization state of a G-protein-coupled receptor CXCR4 by BiFC/BRET. (2008)
  • Author(s): Makiko Hamatake, Yuko Futahashi, Toru Aoki, Naoki Yamamoto, Jun Komano.
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] P-TEFb complex-interacting domain of Brd4 inhibits HIV-1 replication through restricting Tat-mediated enhancement of LTR promoter activity. (2008)
  • Author(s): Emiko Urano, Yumi Kariya, Makiko Hamatake, Hidesuke Fukazawa, Yuko Morikawa, Yoshio Koyanagi, Naoki Yamamoto, Jun Komano.
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] Substitution of the myristoylation signal of HIV-1 Pr55Gag with PLC delta 1 pleeckstrin homology domain results in fully infectious pseudovirion production. (2008)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Makiko Hamatake, Kazuo Terashima, Hirokazu Tamamura, Tsutomu Murakami, Yuko Morikawa, Naoki Yamamoto, Jun Komano.
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] Upregulating expression of cyclin K/CPR4 limits the replication of HIV-1 (2007)
  • Author(s): Emiko Urano, Saki Shimizu, Makiko Hamatake, Yuko Morikawa, Naoki Yamamoto, Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] CD63 and its mutants inhibit fusion of CXCR4-containing vesicles to the plasma membrane and block X4 HIV-1 entry (2007)
  • Author(s): Takeshi Yoshida, Yuji Kawano, Yoshinori Ando, Kei Sato, Jun Komano, Yoshiharu Miura, Yuetsu Tanaka, Yoshio Koyanagi
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] RErouting the plasma membrane targeting of HIV-1 and MLV gag by replacing myristoylation signal with membrane proteins (2007)
  • Author(s): Toru Aoki, Saki Shimiz, Urano Emiko, Yuko Futahashi, Makiko Hamatake, Kazuo Terashima, Tsutomu Murakami, Naoki Yamamoto, Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Analysis of interaction between HIV-1 Gag and tip47 and its associated proteins (2007)
  • Author(s): Kei Miyagawa, Tsutomu Murakami, Yuki Ohsaki, Jun Komano, Toyoshi Fujimto, Naoki Yamamoto
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] the specific phase of membrane-spanning helix of hiv-1 gp41 is critical for intracellular transport of env (2007)
  • Author(s): Kosuke Miyauchi, Rachael Curran, Erin Matthews, Jun Komano, Tsutomu Murakami, Naoki Yamamoto, Donald M Engelman, Zene Matsuda.
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Characterization of neutralizing antibodies purified from Japanese LTNP hemophiliacs (2007)
  • Author(s): Jun Komano
  • Organizer: US-Japan Cooperative Medical Science Program 20th Joint Meeting of the AIDS Panels
  • Place of Presentation: Monterey, CA
• [Presentation] コンピュータ支援によるHIV-1治療薬の開発 (2007)
  • Author(s): 藤秀義、辰巳絢子、栗田明宙、駒野淳、星野忠次
  • Organizer: レトロウイルス研究会夏期セミナー2007
  • Place of Presentation: 河口湖
• [Presentation] HIV-1インテグラーゼ阻害活性を有する6アミノ酸モチーフの同定とその抗ウイルス活性 (2007)
  • Author(s): 濱武牧子駒野淳浦野恵美子厳馬華中原徹堤浩宮内浩典森川裕子玉村啓和杉浦亙山本直樹
  • Organizer: 熊本エイズセミナー
  • Place of Presentation: 熊本
• [Presentation] HIV-1インテグラーゼ阻害活性を有する6アミノ酸モチーフの同定とその抗ウイルス活性 (2007)
  • Author(s): 駒野淳浦野恵美子厳馬華中原徹堤浩濱武牧子宮内浩典森川裕子玉村啓和杉浦亙山本直樹
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Cyclin KICPR4によるHIV-1複製抑制とそのメカニズムの解析 (2007)
  • Author(s): Emiko Urano, Saki Shimizu, Makiko Hamatake, Yuko Morikawa, Naoki Takahashi, Hidesuke Fukazawa, Naoki Yamamoto, Jun Komano
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Gagタンパク質の形質膜輸送シグナルがミリストイル化であることのウイルス学的意義について (2007)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Hirokazu Tamamura, Kazuo Terashima, Tsutomu Murakami, Naoki Yamamoto, Jun Komano
  • Organizer: 第21回日本エイズ学会学術集会
  • Place of Presentation: 広島
• [Presentation] Co-chaperoneタンパク質DNAJ/HSP40familyによるHIV-1複製抑制 (2007)
  • Author(s): 浦野恵美子、奥長造之、森川裕子、駒野淳
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] Biophysical analysis of homotypic interaction facets that mediate the clustering of the G-protein-coupled receptor CXCR4 in the absence of SDF-lalpha (2007)
  • Author(s): 浜武牧子、二橋悠子、青木徹、山本直樹、駒野淳
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
  • Place of Presentation: 横浜
• [Presentation] 非ミリストイル化Gagを用いたレトロウイルスGagのVps輸送経路を通過することによる影響およびそのウイルス学的意義 (2007)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Hirokazu Tamamura, Kazuo Terashima, Tsutomu Murakami, Naoki Yamamoto, Jun Komano
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
  • Place of Presentation: 横浜
• [Presentation] Upregulating expression of cyclin K/CPR4 limits the replication of HIV-1 (2007)
  • Author(s): Emiko Urano, Saki Shimizu, Makiko Hamatake, Yuko Morikawa, Naoki Yamamoto, and Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] CD63 AND ITS MUTANTS INHIBIT FUSION OF CXCR4-CONTAINING VESICLES TO THE PLASMA MEMBRANE AND BLOCK X4HIV-IENTRY (2007)
  • Author(s): Takeshi Yoshida, Yuji Bawano, Yoshinori Ando, Kei Sato, Jun Komano, Yoshiharu Miura, Yuetsu Tanaka and Yoshio Royanagi
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Rerouting the plasma membrane targeting of HIV-1 and MLV gag by replacing myristoylation signal with membrane proteins (2007)
  • Author(s): Toru Aoki, Saki Shimiz, Urano Emiko, Yuko Futahashi, Makiko Hamatake, Kazuo Terashima, Tsutomu Murakami, Naoki Yamamoto, and Jun Komano
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Analysis of interaction between HIV-1 Gag and tip47 and its associated proteins (2007)
  • Author(s): Kei Miyagawa, Tsutomu Murakami, Yuki Ohsaki, Jun Romano, Toyoshi Fujimto and Naoki Yamamoto
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] The specific phase of membrane-spanning helix of hiv-1 gp41 is critical for intracellular transport of env (2007)
  • Author(s): Rosuke Miyauchi, Rachael Curran, Erin Matthews, Jun Komano, Tsutomu Murakami, Naoki Yamamoto, Donald M Engelman, and Zene Matsuda
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Characterization of neutralizing antibodies purified from Japanese LTNP hemoplliliacs (2007)
  • Author(s): Jun Komano
  • Organizer: US-Japan Cooperative Medical Science Program 20th Joint Meeting of AIDS Panels
  • Place of Presentation: Monterey, CA, USA
• [Presentation] Cyclin K/CPR4によるHIV-1複製抑制とそのメカニズムの解析 (2007)
  • Author(s): Emiko Urano, Saki Shimizu, Makiko Hamatake, Yuko Morikawa, Naoki Takahashi, Hidesuke Fukazawa, Naoki Yamamoto, and Jun Komano
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Gagタンパク質の形質膜輸単シグナルがミリストイル化であることのウイルス学的意義について (2007)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Hirokazu Tamamura, Kazuo Terashima, Tsutomu Murakami, Naoki Yamamoto and Jun Komamo
  • Organizer: 第21回日本エイズ学会学術集会
  • Place of Presentation: 広島
• [Presentation] Biophysical analysis of homotypic interaction facets that mediate the clustering of the G-protein-coupled receptor CXCR4 in the absence of SDF-lalpha (2007)
  • Author(s): 浜武 牧子、二橋 悠子、青木 徹、山本 直樹、駒野 淳
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同 大会
  • Place of Presentation: 横浜
• [Presentation] Co-chaperoneタンパク質DNA J/HSP40 familyによるHIV-1複製抑制 (2007)
  • Author(s): 浦野 恵美子、奥長 造之、森川 裕子、駒野 淳
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] 非ミリストイル化Gagを用いたレトロウイルスGagのVps輸送経路を通過することによる影響およびそのウイルス学的意義 (2007)
  • Author(s): Toru Aoki, Saki Shimizu, Emiko Urano, Yuko Futahashi, Makiko Hamatake, Hirokazu Tamamura, Kazuo Terashima, Tsutomu Murakami, Raoki Yamamotoand Jun Romano
  • Organizer: 第30回日本分子生物学会年会・第80園日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] Inhibiting HIV-1 replication by HEXIM1, a cellular inhibitor of cdk9/cyclinT complex (P-TEFb) (2006)
  • Author(s): Saki Shimizu, Emiko Urano, Jun Komano, Yuko Futahashi, Kosuke Miyauchi, Maya Isogai, Zene Matsuda, Kyoko Nohtomi, Kazunari Onogi, Yutaka Takebe, Naoki Yamamoto
  • Organizer: CSH Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Characterization of neutralizing antibodies found in long-term non-progressors of Japanese hemophiliacs (2006)
  • Author(s): Jun Komano
  • Organizer: 3rd Taiwan-Japan Symposium on HIV/AIDS, Center for Disease Control Department of Health
  • Place of Presentation: Taiwan, R.O.C.
• [Presentation] Broadly reactive strong neutralizing antibody against HIV-1 in long-term survivors of HIV-1-infected haemophiliacs (2006)
  • Author(s): Jun Komano
  • Organizer: The US-Japan Cooperative Medical Science Program 19th Joing Meeting of AIDS Panel
  • Place of Presentation: Kagoshima
• [Presentation] Inhibiting HIV-1 replication by HEXIM1, a cellular inhibitor of cdk9/cyclinT complex (P-TEFb) (2006)
  • Author(s): Saki Shimizu, Emiko Urano, Jun Komano, Yuko Futahashi, Kosuke Miyauchi, Maya Isogai, Zene Matsuda, Kyoko Nohtomi, Kazunari Onogi, Yutaka Takebe, Naoki Yamamoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress in conjunction with 79th Annual Meeting of the Japanese Biochemical Society and 29th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: 京都
• [Presentation] Alteration of intracellular transport of the envelope protein of HIV-1 by a shift in a helical phase within its membrane-spanning domain (2006)
  • Author(s): Kosuke Miyauchi, Rachael Curran, Erin Mathews, Jun Komano, Tsutomu Murakami, Naoki Yamamoto, Don M. Engelman, Zene Matsuda.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress in conjunction with 79th Annual Meeting of the Japanese Biochemical Society and 29th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: 京都
• [Presentation] Development of HIV-1 RNaseH inhibitor by Computer-Assisted Drug Design (2006)
  • Author(s): Fuji, H., Tatsumi, J., Komano, A., Hoshino, T.
  • Organizer: Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 沖縄
• [Presentation] Inhibiting HIV-1 replication by HEXIM1, a cellular inhibitor of CDK9/Cyclin T complex (P-TEFb) (2006)
  • Author(s): Saki Shimizu, Yutaka Takebem Emiko Urano, Yuko Futahashi, Kosuke Miyauchi, Maya Isogai, Zene Matsuda, Kyoko Nohtomi, Kazunari Onogi, Naoki Yamamoto, Jun Komano
  • Organizer: 7th AIDS Seminar in Kumamoto
  • Place of Presentation: 熊本
• [Presentation] HIV-1 GagN末端のミリスチン酸化非依存的な分子集合・出芽およびVLPの性質に関する解析 (2006)
  • Author(s): 青木徹, 貝の瀬由成, 二橋悠子, 清水佐紀, 松田善衛, 山本直樹, 駒野淳
  • Organizer: 第54回日本ウイルス学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] AIDS長期未発症のHIV感染血友病患者における高力価中和抗体の存在とその病期進行への寄与に関する解析 (2006)
  • Author(s): 濱武牧子, 浦野恵美子, 花房忠次, 加藤真吾, Tee Kok Keng, 武部豊, 山本直樹, 駒野淳
  • Organizer: 第54回日本ウイルス学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] 挿入変異を伴う多剤耐性HIV-1(CRF01_AE)における薬剤耐性亢進のメカニズムー薬剤耐性獲得におけるRNase H活性の関与 (2006)
  • Author(s): 駒野淳, 二橋悠子, 磯貝まや, 濱武牧子, 松田善衛, 佐藤裕徳, 椎野貞一郎, 武部豊, 山本直樹
  • Organizer: 第54回日本ウイルス学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] HIV-1の逆転写酵素が持つRNase H活性に対する特異的阻害剤の開発 (2006)
  • Author(s): 駒野淳, 姉崎裕介, 二橋悠子, 磯貝まや, 藤義秀, 星野忠次, 武部豊, 山本直樹
  • Organizer: 第54回日本ウイルス学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] Myristoylation independent assembly, transport, and VLP formation of HIV-1 Gag (2006)
  • Author(s): Komano J.
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] HIV-1の逆転写酵素に内在するRNase H活性阻害薬の開発(1)-小分子化合物ライブラリーからのスクリーニング (2006)
  • Author(s): 駒野淳, 姉崎裕介, 二橋悠子, 磯貝まや, 武部豊, 山本直樹
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] 血友病患者におけるエイズ長期未発症症例における高力価中和抗体の存在と標的部位の同定 (2006)
  • Author(s): 濱武牧子, 浦野恵美子, 花房忠次, 加藤真吾, 田eeKokKeng, 武部豊, 山本直樹, 駒野淳
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] HIV-1逆転写酵素polymerase active siteへの薬剤耐性変異が誘導するRNase H活性の低下と耐性亢進への寄与 (2006)
  • Author(s): 駒野淳, 二橋悠子, 磯貝まや, 濱武牧子, 松田善衛, 佐藤裕徳, 椎野貞一郎, 武部豊, 山本直樹
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] HIV-1の逆転写酵素に内在するRNase H活性阻害薬の開発-in silico解析による作用機序解析と最適化の試み- (2006)
  • Author(s): 藤秀義, 辰巳絢子, 駒野淳, 星野忠次
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] Inhibition of HIV-1 replicstion by co-chaperone DNA J/HSP40 protein family
  • Author(s): 浦野恵美子, 奥長浩之、森川裕子、山本直樹、駒野淳
  • Organizer: 第22回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪