Identifcataion of new molecular targets by analysis of multiple DNA repair activity of familial breast cancer susceptibility gene

• Project/Area Number: 18689032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Tohoku University
• Principal Investigator: CHIBA Natsuko Tohoku University, 加齢医学研究所, 准教授 (50361192)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2006: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: がん / DNA修復 / 乳がん / BRCA1 / 癌

Research Abstract

家族性乳癌原因遺伝子BRCA1は、その生殖細胞系列変異により、乳癌、卵巣癌を引き起こす癌抑制遺伝子である。本研究ではBRCA1のDNA修復能に関する機能を詳細に解析し、BRCA1がDNA二本鎖切断、紫外線照射後の転写共役修復、さらには細胞分裂に関与することを明らかにした。これらの研究成果は、家族性乳癌卵巣癌に加え、散発性の乳癌、卵巣癌の発症機構の解明、新たな治療薬となる分子標的の探索、さらには乳癌、卵巣癌の予防法解明にも寄与すると考えられる。

Research Products

• [Journal Article] Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80 (2008)
  • Author(s): Wei L, Lan L, Hong Z, Yasui A, Ishioka C, and Chiba N
  • Journal Title: Mol. Cell. Biol 28(24) Pages: 7380-7393
  • Peer Reviewed
• [Journal Article] Rapid recruitment of BRCA1 to DNA double-strand breaks is denendent on its association with Ku80 (2008)
  • Author(s): Wei Leizhen
  • Journal Title: Molecular and Cellular Biology 28(24) Pages: 7380-7393
  • Peer Reviewed
• [Journal Article] 家族性乳癌の原因遺伝子 (2007)
  • Author(s): 千葉奈津子,石岡千加史
  • Journal Title: 日本臨床2007年増刊 乳癌ー基礎・臨床のアップデート 65(6) Pages: 601-605
• [Journal Article] 生殖細胞変異の遺伝子診断-家族性乳癌BRCA1,BRCA2を中心に (2007)
  • Author(s): 千葉奈津子,下平秀樹,石岡千加史
  • Journal Title: 実験医学 25(17)(増刊)
• [Journal Article] 家族性乳癌の原因遺伝子 (2007)
  • Author(s): 千葉奈津子
  • Journal Title: 日本臨床 増刊 65(6) Pages: 601-605
• [Journal Article] 原発不明癌22例の検討 (2007)
  • Author(s): 加藤誠之
  • Journal Title: 癌と化学療法 34(8) Pages: 1227-1231
  • Peer Reviewed
• [Journal Article] 生殖細胞変異の遺伝子診断-家族性乳癌BRCA1, BRCA2を中心に (2007)
  • Author(s): 千葉奈津子
  • Journal Title: 実験医学 増刊 25(17) Pages: 2629-2634
• [Journal Article] A dose escalation study of docetaxel and nedaplatin in patients with relapsed or refractory squamous cell carcinoma of the esophagus pretreated using cisplatin, 5-fluorouracil and radiation (2006)
  • Author(s): Yoshioka T , Sakayori M, Kato S, Chiba N, Miyazaki S, Nemoto K, Shibata H, Shimodaira H, Ohtsuka K, Kakudo Y, Sakata Y, and Ishioka C
  • Journal Title: Int J Clin Oncol 11(6) Pages: 454-460
  • NAID: 10020571819
  • Peer Reviewed
• [Journal Article] 家族性乳癌の遺伝子検査に関わる問題点 (2006)
  • Author(s): 酒寄真人, 千葉奈津子, 河原正典, 白石千子, 武田元博, 野水整, 野口眞三郎, 大内憲明, 竹之下誠一, 石岡千加史
  • Journal Title: 家族性腫瘍 6(1) Pages: 7-11
  • NAID: 130007540991
  • Peer Reviewed
• [Journal Article] Weekly paclitaxel療法が奏効した食道原発悪性黒色腫の1例 (2006)
  • Author(s): 角道祐一
  • Journal Title: 癌と化学療法 33・7 Pages: 969-972
• [Journal Article] A dose escalation study of docetaxel and nedaplatin in patients with relapsed or refractory squamous cell carcinoma of the esophagus pretreated using cisplatin, 5-fluorouracil and radiation (2006)
  • Author(s): Takashi Yoshioka
  • Journal Title: International Journal of Clinical Oncology 11・6 Pages: 454-460
• [Presentation] 家族性乳癌原因遺伝子BRCA1の紫外線損傷への集積 (2008)
  • Author(s): 千葉奈津子, 魏雷震, 蘭利, 佐竹正延, 安井明, 石岡千加史
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] 家族性乳癌原因遺伝子BRCA1の紫外線損傷への集積 (2008)
  • Author(s): 千葉奈津子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] Tumor suppressor BRCA1 response to DNA single-strand breaks induced by laser micro-irradiation (2008)
  • Author(s): Natsuko Chiba
  • Organizer: Th7^<th> Internatopnal Symposium on Nano-Biomedical Engineering (ISNBME-7)
  • Place of Presentation: Tainan, Taiwan
  • Year and Date: 2008-10-16
• [Presentation] Rapid accumulation of BRCA1 at DNA double-strand breaks is denendent on Ku80 (2008)
  • Author(s): Wei Leizhen
  • Organizer: Ataxia-Telamgiectasia Workshop 2008
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2008-04-25
• [Presentation] Tumor suppressor BRCA1 response to DNA single-strand breaks induced by laser micro-irradiation (2008)
  • Author(s): Chiba N. and Wei L
  • Organizer: The 7th Internatopnal Symposium on Nano-Biomedical Engineering (ISNBME-7)
  • Place of Presentation: Tainan, Taiwan
• [Presentation] Rapid accumulation of BRCA1 at DNAdouble-strand breaks is dependent on Ku80 (2008)
  • Author(s): Wei L, Lan L., Hong Z, Yasui A, Satake M, Ishioka C. and Chiba N
  • Organizer: Ataxia-Telamgiectasia Workshop 2008
  • Place of Presentation: Ohtsu Japan
• [Presentation] Recruitment of Tumor Suppressor, BRCA1 to DNA double strands induced by lasesr micro irradiation (2008)
  • Author(s): Chiba N
  • Organizer: 5th International Symposium of 2007 Tohoku University Global COEProgram Global Nano-Biomedical Engineering Education and Research Network Centre, Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Matsushima Japan
• [Presentation] 家族性乳癌原因遺伝子BRCA1のN末端のDNA二本鎖切断への集積 (2007)
  • Author(s): 魏雷震, 千葉奈津子, 蘭利, 佐竹正延, 安井明, 石岡千加史
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] Domain-dependent accumulation of BRCA1 at the sites of DNA damage (2007)
  • Author(s): Chiba N., Wei L, Lan L., Hong Z, Yasui A and Ishioka C
  • Organizer: AACR-NCI-EORTC International conference Molecular targets and Cancer Therapeutics
  • Place of Presentation: San Francisco U.S.A
• [Presentation] Two cases of breast cancer responding to trastuzumab monotherapy (2007)
  • Author(s): Chiba N, Yoshioka T, Takahashi S, Shibata H, Kato S, Shimodaira H, Otsuka K, Kakudo Y, Takahashi M, Yasuda K, Sakamoto Y, and Ishioka C
  • Organizer: Asia Pacific Medical Education Initiative, 3rd Asia Pacific Workshop, Molecular targeted Therapy of Cancer
  • Place of Presentation: Shanghai China
• [Presentation] Domain-dependent accumulation of BRCA1 at the sites of DNA damage (2007)
  • Author(s): Chiba N.
  • Organizer: AACR-NCI-EORTC International conference Molecular targets and Cancer Therapeutics
  • Place of Presentation: San Francisco U.S.A.
• [Presentation] リアルタイム分析による家族性乳癌原因遺伝子BRCA1のDNA損傷への集積 (2006)
  • Author(s): 魏雷震, 千葉奈津子, 蘭利, 安井明, 石岡千加史
  • Organizer: 第65回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2006-09-28
• [Book] 第12章 癌、癌化:ウイルス発癌、第13章 癌化のシグナル伝達 細胞・培地活用ハンドブック (2007)
  • Author(s): 秋山徹, 河府和義編
  • Publisher: 羊土社
• [Book] 細胞・培地活用ハンドブック (2007)
  • Author(s): 秋山徹
  • Publisher: 羊土社
• [Book] 第26章 主な制癌剤 阻害剤活用ハンドブック (2006)
  • Author(s): 秋山徹, 河府和義編
  • Publisher: 羊土社
• [Book] 阻害剤活用ハンドブック (2006)
  • Author(s): 秋山徹
  • Total Pages: 468
  • Publisher: 羊土社