Identification of new methylation markers for early diagnosis and therapy development in pancreatic cancer

• Project/Area Number: 18689033
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: NORIHIRO Sato Kyushu University, 大学院・医学研究院, 助教 (20423527)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2006: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
• Keywords: 膵癌 / メチレーション / マーカー / PanIN / マイクロアレイ

Research Abstract

我々はマイクロアレイによる膵癌メチル化遺伝子のスクリーニングと実際の膵癌細胞でのメチル化状態を調査し、新規メチル化ターゲットを同定した。遺伝子reelin(RELN)、Reprimo、SPARCはそれぞれ膵癌で異常メチル化による発現低下を認め、それぞれが、膵癌の浸潤などの悪性度に深く関与する一方、予後因子として臨床応用可能であることが示唆された。また、DNAメチル化異常は膵癌の前駆病変(PanIN)の進展に伴いメチル化の程度が増加しており、膵発癌との関与が示唆された。

Research Products

• [Journal Article] CpG island methylation profile of pancreatic intraepithelial neoplasia (2008)
  • Author(s): Sato N, et al
  • Journal Title: Mod Pathol 21 Pages: 238-44
  • Peer Reviewed
• [Journal Article] CpG island methylation profile of pancreatic intraepithelial neoplasia. (2008)
  • Author(s): Sato, N, et. al.
  • Journal Title: Mod Pathol. 21 Pages: 238-44
  • Peer Reviewed
• [Journal Article] Peritumoral SPARC expression and patient outcome with resectable pancreatic adenocarcinoma (2007)
  • Author(s): Infante JR, Sato N et al
  • Journal Title: J Clin Oncol 25 Pages: 319-25
  • Peer Reviewed
• [Journal Article] Peritumoral SPARC expression and patient outcome with resectable pancreatic adenocarcinoma. (2007)
  • Author(s): Infante, JR・Sato, N, et. al.
  • Journal Title: J Clin Oncol 25 Pages: 319-25
  • Peer Reviewed
• [Journal Article] Differential and epigenetic gene expression profiling identifies frequent disruption of the RELN pathway in pancreatic cancers (2006)
  • Author(s): Sato N et al
  • Journal Title: Gastroenterology 130 Pages: 548-65
  • Peer Reviewed
• [Journal Article] Aberrant methylation of Reprimo correlates with genetic instability and predicts poor prognosis in pancreatic ductal adenocarcinoma (2006)
  • Author(s): Sato N et al
  • Journal Title: Cancer 107 Pages: 251-7
  • Peer Reviewed
• [Journal Article] DNA methylation alterations in the pancreatic juice of patients with suspected pancreatic disease (2006)
  • Author(s): Sato N et al
  • Journal Title: Cancer Res 66 Pages: 1208-11
  • Peer Reviewed
• [Journal Article] The role of epigenetic alterations in pancreatic cancer (2006)
  • Author(s): Sato N et al
  • Journal Title: J Hepatobiliary Pancreat Surg 13 Pages: 286-95
  • Peer Reviewed
• [Journal Article] Epigenetic alterations in intraductal papillary mucinous neoplasms of the pancreas (2006)
  • Author(s): Sato N et al
  • Journal Title: J Hepatobiliary Pancreat Surg 13 Pages: 280-5
  • Peer Reviewed
• [Journal Article] Differential and epigenetic gene expression profiling identifies frequent disruption of the RELN pathway in pancreatic cancers. (2006)
  • Author(s): Sato N et al.
  • Journal Title: Gastroenterology. 130(2) Pages: 548-565
• [Journal Article] Aberrant methylation of Reprimo correlates with genetic instability and predicts poor prognosis in pancreatic ductal adenocarcinoma. (2006)
  • Author(s): Sato N et al.
  • Journal Title: Cancer 107(2) Pages: 251-257
• [Journal Article] DNA methylation alterations in the pancreatic juice of patients with suspected pancreatic disease. (2006)
  • Author(s): Sato N et al.
  • Journal Title: Cancer Res. 66(2) Pages: 1208-1211
• [Journal Article] The role of epigenetic alterations in pancreatic cancer. (2006)
  • Author(s): Sato N et al.
  • Journal Title: J Hepatobiliary Pancreat Surg. 13(4) Pages: 286-295
• [Journal Article] Epigenetic alterations in intraductal papillary mucinous neoplasms of the pancreas. (2006)
  • Author(s): Sato N et al.
  • Journal Title: J Hepatobiliary Pancreat Surg. 13(4) Pages: 280-285
• [Presentation] Gemcitabine Enhances the Effect of Adenovirus-mediated Gene Therapy (2008)
  • Author(s): Manabu Onimaru, Kenoki Ohuchida, Eishi Nagai, Kazuhiro Mizumoto, Takuya Egami, Norihiro Sato
  • Organizer: 39th Annual Meeting of the American Pancreatic Association
  • Place of Presentation: Masao Tanaka
• [Presentation] CD133 positive cells in pancreatic cancer possess increased cell proliferation, migration and invasion (2008)
  • Author(s): Moriyama T, Ohuchida K, Mizumoto K, Lin C, Sato N, Tanaka M
  • Organizer: the 39th Annual Meeting of the American Pancreatic Association
  • Place of Presentation: Tanaka M
• [Presentation] 「膵癌におけるSPARC発現消失とその生物学的意義」九州大学医学部臨床腫瘍外科
  • Author(s): 三好圭,佐藤典宏
  • Organizer: 第107回日本外科学会学術総会
  • Place of Presentation: 水元一博ら
• [Presentation] PREOPERATIVE DIAGNOSIS OF TUMOR EXTENT OF BILE DUCT CANCER BY INTRADUCTAL ULTRASONOGRAPHY.
  • Organizer: 3rd. Annual ACADEMIC SURGICAL CONGRESS.