• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of a novel Nucleolar protein complex.

Research Project

Project/Area Number 18689902
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

MURAYAMA Akiko  University of Tsukuba, 大学院・生命環境科学研究科, 講師 (50431656)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥26,200,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2008: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2006: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywords核小体 / がん治療 / クロマチン / DNAメチル化酵素 / rRNA / 癌 / 酵素 / 発現制御 / ゲノム
Research Abstract

Nucleomethylin(NML)という新規核小体蛋白質が細胞培養液中のグルコースを減らすと、ヒト培養細胞中のリボソーム合成やたんぱく質産生量が低下することを見出した。NMLは核小体に存在し、NAD依存的脱アセチル化酵素SIRT1やヒストンH3K9メチル化酵素SUV39H1と複合体を形成する。NML複合体はrDNA領域をヘテロクロマチン化することによって栄養飢餓状態でのrRNA合成抑制に働くことを明らかにした。また、NML複合体の機能破綻はエネルギーバランスの崩壊と、それに伴う細胞死を引き起こすことから、栄養飢餓による細胞死の回避に働いていることも判明した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (32 results)

All 2010 2009 2008 2007 2006 Other

All Journal Article (16 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Estrogen inhibits TGF-{beta} signaling by promoting Smad2/3 degradation.2010

    • Author(s)
      Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, Hirota R, Murayama A, Kimura K, Imamura T, Yanagisawa J
    • Journal Title

      The Journal of biological chemistry (In press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The chromosomal association of condensin II is regulated by a noncatalytic function of PP2A.2009

    • Author(s)
      Takemoto A, Maeshima K, Ikehara T, Yamaguchi K, Murayama A, Imamura S, Imamoto N, Yokoyama S, Hirano T, Watanabe Y, Hanaoka F, Yanagisawa J, Kimura K
    • Journal Title

      Nature Structural & Molecular Biology Vol.16,No.12

      Pages: 1302-1308

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The nucleolar protein NML regulates hepatic ATP levels during liver regeneration after partial hepatectomy.2009

    • Author(s)
      Mikogai A, Yanagisawa J, Yasuzawa-Tanaka K, Murayama A
    • Journal Title

      Biochemical and Biophysical Research Communications Vol.390,No3

      Pages: 591-596

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] KLF4 suppresses estrogen-dependent breast cancer growth by inhibiting the transcriptional activity of ERα.2009

    • Author(s)
      Akaogi K, Nakajima Y, Ito I, Kawasaki S, Oie S, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Oncogene Vol.28,No32

      Pages: 2894-2902

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways.2009

    • Author(s)
      Kajiro M, Hirota R, Nakajima Y, Kawanowa K, So-ma K, Ito I, Yamaguchi Y, Ohie S, Kobayashi Y, Seino Y, Kawano M, Kawabe YI, Takei H, Hayashi S, Kurosumi M, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Nature Cell Biol. Vol.11,No3

      Pages: 312-319

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The nucleolar protein NML regulates hepatic ATP levels during liver regeneration after partial hepatectomy.2009

    • Author(s)
      Mikogai A, Yanagisawa J, Yasuzawa-Tanaka K, Murayama A.
    • Journal Title

      Biochem Biophys Res Commun. 390(3)

      Pages: 591-596

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic control of rDNA loci in response to intracellular energy status.2008

    • Author(s)
      Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Simizu T, Yanagisawa J
    • Journal Title

      Cell. Vol.133

      Pages: 627-639

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] PPARγligands suppress the feedback loop between E2F2 and cyclin-E1.2008

    • Author(s)
      Komatsu Y, Ito I, Wayama M, Fujimura A, Akaogi K, Machida H, Nakajima Y, Kuroda T, Ohmori K, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Biochem Biophys Res Commun. Vol.370,No.1

      Pages: 145-148

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic control of rDNA loci in response to intracellular energy status2008

    • Author(s)
      Akiko Murayama, Kazuji Omori, Akiko Fujimura, Hiroshi Minami, Kayoko Yasazawa-Tanaka, Takao Kuroda, Shohei Oie, Hiroaki Daitoku, Mitsuru Okuwaki, Kyosuke Nagata, Akiyoshi Fukamizu, Keiji Kimura, Toshiyuki Shimizu, Junn Yanagisawa
    • Journal Title

      Cell 133

      Pages: 627-639

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensing I.2007

    • Author(s)
      Takemoto A, Murayama A, Katano M, Urano T, Furukawa K, Yokoyama S, Yanagisawa J, Hanaoka F, Kimura K
    • Journal Title

      Nucleic Acids Res. Vol.35

      Pages: 2403-2412

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Turning Off Estrogen Receptor β-Mediated Transcription Requires Estrogen-Dependent Receptor Proteolysis.2006

    • Author(s)
      Tateishi Y, Sonoo R, Sekiya Y, Sunahara N, Kawano M, Wayama M, Hirota R, Kawabe YMurayama A, Kato S, Kimura K, Yanagisawa J
    • Journal Title

      Mol. Cell. Biol. Vol.26

      Pages: 7966-7976

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A specific CpG site demethylation in the human interleukin 2 gene promoter is an epigenetic memory.2006

    • Author(s)
      Murayama A, Sakura K, Nakama M, Yasuzawa-Tanaka K, Fujita E, Tateishi Y, Wang Y, Ushijima T, Shibuya K, Kawabe Y, Yanagisawa J
    • Journal Title

      EMBO J. Vol.25

      Pages: 1081-1092

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Negative regulation of condensing 1 by CK2-mediated phosphorylation.2006

    • Author(s)
      Takemoto A, Kimura K, Yanagisawa J, Yokoyama S, Hanaoka F.
    • Journal Title

      EMBO J 25

      Pages: 5339-5348

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Turning Off Estrogen Receptor β-Mediated Transcription Requires Estrogen-Dependent Receptor Proteolysis.2006

    • Author(s)
      Tateishi Y, Sonoo R, Sekiya Y, Sunahara N, Kawano M, Wayama M, Hirota R, Kawabe Y, Murayama A, Kato S, Kimura K, Yanagisawa J.
    • Journal Title

      Molecular and Cellular Biology 26.21

      Pages: 7966-7976

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A specific CpG site demethylation in the human interleukin 2 gene promoter is an epigenetic memory.2006

    • Author(s)
      Murayama A, Sakura K, Nakama M, Yasuzawa-Tanaka K, Fujita E, Tateishi Y, Wang Y, Ushijima T, Shibuya K, Kawabe Y, Yanagisawa J.
    • Journal Title

      EMBO J. 25

      Pages: 1081-1092

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification2006

    • Author(s)
      Oishi H, Kitagawa H, Wada O, Takezawa S, Tora L, Kouzu-Fujita M, Takada I, Yano T, Yanagisawa J, Kato S
    • Journal Title

      J Biol Chem. 281(1)

      Pages: 20-26

    • Related Report
      2006 Annual Research Report
  • [Presentation] 核小体因子Nucleomethylinによるリボソーム合成調節機構と細胞内エネルギー代謝2010

    • Author(s)
      村山明子
    • Organizer
      2010年度日本農芸化学会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] 核小体因子Nucleomethylinによるリボソーム合成調節機構と細胞内エネルギー代謝2010

    • Author(s)
      村山明子
    • Organizer
      2010年度 日本農芸化学会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス肝臓における核小体因子Nucleomethylinの機能解析2009

    • Author(s)
      御子貝綾、村山明子、安澤(田中)加代子、柳澤純
    • Organizer
      第32回日本分子生物学会年会・横浜
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] エピジェネティック機構を介した細胞内エネルギー代謝制御2009

    • Author(s)
      村山明子
    • Organizer
      日本レチノイド研究会第20回学術集会・ランチョンセミナー
    • Place of Presentation
      東京
    • Year and Date
      2009-11-21
    • Related Report
      2008 Final Research Report
  • [Presentation] エピジェネティック機構を介した細胞内エネルギー代謝制御2009

    • Author(s)
      村山明子
    • Organizer
      日本レチノイド研究会第20回学術集会・ランチョンセミナー・東京
    • Place of Presentation
      東京
    • Year and Date
      2009-11-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 核小体因子Nucleomethylinによる核内反応制御機構の解析2009

    • Author(s)
      村山明子
    • Organizer
      第82回日本生化学会大会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 核小体因子Nucleomethylinによる核内反応制御機構の解析2009

    • Author(s)
      村山明子
    • Organizer
      第82回日本生化学会大会 シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] rDNA領域のエピジェネティックな制御による細胞内エネルギー調節機構2008

    • Author(s)
      村山明子
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会・シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] rDNA領域のエピジェネティックな制御による細胞内エメルギー調節機構2008

    • Author(s)
      村山明子
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会・シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] a novel protein complex, senses intracellular energy status and epigenetically controls the rDNA locus.2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Shohei Oie, Toshiyuki Shimizu, Junn Yanagisawa eNoSC
    • Organizer
      第21回内藤コンファレンス
    • Place of Presentation
      八ヶ岳
    • Year and Date
      2008-06-26
    • Related Report
      2008 Final Research Report
  • [Presentation] eNoSC, a novel protein comples, senses intracellular energy status and epigenetically controls the rDNA locus.2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Shohei Oie, Toshiyuki Shimizu, Junn Yanagisawa
    • Organizer
      第21回内藤コンファレンス
    • Place of Presentation
      八ヶ岳
    • Year and Date
      2008-06-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] a novel protein complex2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Takao Kuroda, Shohei Oie, Toshiyuki Shimizu, Junn Yanagisawa eNoSC
    • Organizer
      epigenetically controls the rDNA locus Metabolic Pathways of Longevity・Keystone Symposium
    • Place of Presentation
      Denver
    • Year and Date
      2008-04-01
    • Related Report
      2008 Final Research Report
  • [Presentation] eNoSC, a novel protein complex, senses intracllilar energy status and epigenetically controls the rDNA locus.2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Takao Kuroda, Shohei Oie, Toshiyuki Shimizu and Junn Yanagisawa
    • Organizer
      Metabolic Pathways of Longevity・Keystone Symposium
    • Place of Presentation
      Denver, アメリカ
    • Year and Date
      2008-04-01
    • Related Report
      2007 Annual Research Report
  • [Book] 代謝ストレス応答とrRNA転写制御機構2009

    • Author(s)
      村山明子
    • Total Pages
      9
    • Publisher
      生化学 第81巻第6号p456-464
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://yanagisawalab.org/

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://yanagisawalab.org/home/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi