Understanding a mechanism of HIV-1 drug resistance development under the RTI pressure and the development of a medication scheduling system

• Project/Area Number: 18700293
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioinformatics/Life informatics
• Research Institution: Toyohashi University of Technology
• Principal Investigator: HARADA Koji Toyohashi University of Technology, 工学部, 助教 (40390504)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,690,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥90,000); Fiscal Year 2008: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
• Keywords: HIV / エイズ / 薬剤耐性 / 逆転写酵素 / ドラッグディスカバリー / 転写エラー / 抗原多様性閾 / 力学系 / 抗原多様性閾値 / コンピュータシミュレーション

Research Abstract

エイズ医療に関する二つの課題に取り組んだ。最初の課題は、エイズ発症機序を数理モデル解析により明らかにすることである。提案モデルの特徴は、HIV逆転写酵素の動的な転写エラー率変化、HIVのもろ刃の剣的状況を考慮した点にある。モデル解析により、転写エラー率に関して、エイズの発症を決定する閾値が存在することを明らかにした。第二の課題は、HIV薬剤耐性化を起こさない薬剤ターゲットの特徴を同定することである。ここでは、ドラッグディスカバリーの分野で注目されているネットワークアプローチによる研究手法を採用した。成果として、複数の宿主因子と相互作用をもつ「ハブ」HIVタンパク質が、薬剤耐性化を起こしにくいということを確率モデルで証明した。

Research Products

• [Journal Article] A hub gene in a HIV-1 gene regulatory network is a promising target for anti-HIV-1 drugs (2009)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Proc.of AROB 14^<th> 09 Pages: 522-525
  • Peer Reviewed
• [Journal Article] A time-dependent threshold condition to determine an onset of AIDS (2009)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Proc.of AROB 14^<th> 09 Pages: 534-537
  • Peer Reviewed
• [Journal Article] A time-dependent threshold condition to determine an onset of AIDS (2009)
  • Author(s): Kouji Harada
  • Journal Title: Proceedings of AROB 14^<th> 09 Pages: 534-537
  • Peer Reviewed
• [Journal Article] A hub gene in a HIV-I gene regulatory network is apromising target for anti-HIV-1 drugs (2009)
  • Author(s): Kouji Harada
  • Journal Title: Proceedings of AROB 14^<th> 09 Pages: 522-525
  • Peer Reviewed
• [Journal Article] Antibody-Based Computing (2008)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Artificial Life and Robotics 12 Pages: 180-183
  • Peer Reviewed
• [Journal Article] Antiobody-Based Computing (2008)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Proc.of AROB 13^<th> 08 Pages: 465-468
  • Peer Reviewed
• [Journal Article] Fatal Mutations in HIV Population as an Influential Factor for an Onset of AIDS (2008)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Lecture Note in n Computer Science LNCS/LNAI 5179 Pages: 432-439
  • Peer Reviewed
• [Journal Article] Antibody-Based Computing (2008)
  • Author(s): Kouji Harada
  • Journal Title: Artificial Life and Robotics 13 Pages: 180-183
  • Peer Reviewed
• [Journal Article] Antibody-Based Computing (2008)
  • Author(s): Kouji Harada
  • Journal Title: Proceedings of AROB 13^<th> 08 Pages: 465-468
  • Peer Reviewed
• [Journal Article] Fatal Mutations in HIV Population as an Influential Factor for an Onset of AIDS (2008)
  • Author(s): Kouji Harada
  • Journal Title: Lecture Notes in Computer Science 5179 Pages: 432-439
  • Peer Reviewed
• [Journal Article] Antibody Based Computation (2008)
  • Author(s): Kouji Harada, Yoshiteru Ishida
  • Journal Title: Proc. of AROB 13th'08 (CD-ROM) Pages: 465-468
  • Peer Reviewed
• [Journal Article] Mutation Probability Threshold of HIV for AIDS (2007)
  • Author(s): Kouji Harada and Yoshiteru Ishida
  • Journal Title: Lecture Notes in Computer Science LNCS/LNAI 4696 Pages: 838-845
  • Peer Reviewed
• [Journal Article] Mutation Probability Threshold of HIV for AIDS (2007)
  • Author(s): Kouji Harada, Yoshiteru Ishida
  • Journal Title: Lecture Note in Computer Science 4694 Pages: 838-845
  • Peer Reviewed
• [Journal Article] Tolerance Dependent on Timing/Amount of Antigen-Dose in an Asymmetric Idiotypic Network (2006)
  • Author(s): Kouji Harada
  • Journal Title: Lecture Notes in Computer Science LNCS/LNAI 4252 Pages: 115-122
  • Peer Reviewed
• [Journal Article] Tolerance Dependent on Timing/Amount of Antigen-Dose in an Asymmetric Idiotypic Network (2006)
  • Author(s): Koji Harada
  • Journal Title: Lecture Note in Computer Science 4252 Pages: 115-122
• [Presentation] A hub gene in a HIV-I gene regulatory network is a promising target for anti-HIV-I drugs (2009)
  • Author(s): Kouji Harada
  • Organizer: AROB 14^<th> 09
  • Place of Presentation: 大分県別府市
  • Year and Date: 2009-02-06
• [Presentation] A time-dependent threshold condition to determine an onset of AIDS (2009)
  • Author(s): Kouji Harada
  • Organizer: AROB 14^<th> 09
  • Place of Presentation: 大分県別府市
  • Year and Date: 2009-02-06
• [Presentation] A hub gene in a HIV-1 gene regulatory network is a promising target for anti-HIV-1 drugs (2009)
  • Author(s): Kouji Harada
  • Organizer: AROB 14^<th> 09
  • Place of Presentation: Beppu, Japan
• [Presentation] A time-dependent threshold condition to determine an onset of AIDS (2009)
  • Author(s): Kouji Harada
  • Organizer: AROB 14^<th> 09
  • Place of Presentation: Beppu, Japan
• [Presentation] Fatal Mutations in HIV Population as an Influential Factor for an Onset of AIDS (2008)
  • Author(s): Kouji Harada
  • Organizer: KES'08
  • Place of Presentation: Zagreb, Croatia
  • Year and Date: 2008-09-05
• [Presentation] Antibody-Based Computing (2008)
  • Author(s): Kouji Harada
  • Organizer: AROB 13^<th> 08
  • Place of Presentation: 大分県別府市
  • Year and Date: 2008-02-01
• [Presentation] Antibody-Based Computing (2008)
  • Author(s): Kouji Harada
  • Organizer: AROB 14^<th> 08
  • Place of Presentation: Beppu, Japan
• [Presentation] Fatal Mutations in HIV Population as an Influential Factor for an Onset of AIDS (2008)
  • Author(s): Kouji Harada
  • Organizer: KES 08
  • Place of Presentation: Zagreb, Croatia
• [Presentation] Mutation Probability Threshold of HIV for AIDS (2007)
  • Author(s): Kouji Harada
  • Organizer: KES 07
  • Place of Presentation: Vietri sul Mare, Italy
• [Presentation] Tolerance Dependent on Timing/Amount of Antigen-Dose in an Asymmetric Idiotypic Network (2006)
  • Author(s): Kouji Harada
  • Place of Presentation: Bournemouth, UK