Loss of the functional-synapse in RA175(SynCAM)-deficient mice

• Project/Area Number: 18700333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: FUJITA Eriko National Center of Neurology and Psychiatry, 疾病研究第5部, 流動研究員 (20291651)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 脳・神経 / シグナル伝達 / SynCAM / 機能シナプス

Research Abstract

免疫グロブリンファミリーに属すRA175(SynCAM1)は、C末端のPDZ結合領域を介して細胞接着・機能的シナプスの形成に関与している。申請者はRA175のC末端EYFIに結合するタンパクを探索し、複合体を形成する蛋白をプルダウン・免疫沈降・免疫染色法および作成したRA175ノックアウトマウスを用いて解析した。その結果、RA175はPar-3と直接結合すること、特異的な神経回路網作製のために過剰なRA175はデンドライト上で切断されている可能性を示唆した。

Research Products

• [Journal Article] Neuronal RA175/SynCAM1 isoforms are processed by tumor necrosis factor-alpha-converting enzyme (TACE)/ADAM17-like proteases. (2008)
  • Author(s): Tanabe Y, Kasahara T, Momoi T, Fujita E
  • Journal Title: Neurosci Lett 444 Pages: 16-21
• [Journal Article] Mutations in the gene encoding CADM1 are associated with autism spectrum disorder. (2008)
  • Author(s): Zhiling Y, Fujita E, Tanabe Y, Yamagata T, Momoi T, Momoi MY
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 926-929
• [Journal Article] Mutations in the gene encoding CADM1 are associated with autism spectrum disorder (2008)
  • Author(s): Zhiling Y, Fujita E, Tanabe Y, Yamagata T, Momoi T, Momoi MY.
  • Journal Title: Biochem Biophys Res Commun. 377 Pages: 926-929
  • Peer Reviewed
• [Journal Article] Neuronal RA175/SynCAM1 isoforms are processed by tumor necrosis factor-alphaーconverting enzyme(TACE)/ADAM17-like proteases (2008)
  • Author(s): Tanabe Y, Kasahara T, Momoi T, Fujita E.
  • Journal Title: Neurosci Lett. 44 Pages: 6-21
  • Peer Reviewed
• [Journal Article] Loss of partitioning-defective-3/isotype-specific interacting protein (par-3/ASIP) in the elongating spermatid of RA175 (IGSF4A/SynCAM)-deficient mice. (2007)
  • Author(s): Fujita E, Tanabe Y, Hirose T, Aurrand-Lions M, Kasahara T, Imhof BA, Ohno S, Momoi T
  • Journal Title: Am J Pathol 171 Pages: 1800-1810
• [Journal Article] Lose of partirioning defective -3/isotype specific interacting protein (par-3/ASIP)in the elongating spermatid of RA175 (IGSF4A/SynCAM)-deficient mice (2007)
  • Author(s): Fujita E, Tanabe Y, Hirose T, Aurrand-Lions M, Kasahara T, Imhof BA, Ohno S, Momoi T.
  • Journal Title: Am J Pathol. 171 Pages: 1800-1810
  • Peer Reviewed
• [Journal Article] Two endoplasmic reticulum-associated degradation (ERAD) systems for the novel variant of the mutant dysferlin : ubiquitin/proteasome ERAD(I) and autophagy/lysosome ERAD(II). (2007)
  • Author(s): Fujita E, Kouroku Y, Isoai A, Kumagai H, Misutani A, Matsuda C, Hayashi YK, Momoi T.
  • Journal Title: Hum Mol Genet. 16・6 Pages: 618-629
• [Journal Article] Oligo-astheno-teratozoospermia in mice lacking RA175/TSLC1/SynCAM/IGSF4A, a cell adhesion molecule in the immunoglobulin superfamily. (2006)
  • Author(s): Fujita E, Kouroku Y, Ozeki S, Tanabe Y, Toyama Y, Maekawa M, Kojima N, Senoo H, Toshimori K, Momoi T
  • Journal Title: Mol Cell Biol 26 Pages: 718-726
• [Journal Article] Oligo-astheno-teratozoospermia in mice lacking RA175/TSLC1/SynCAM/IGSF4A, a cell adhesion molecule in the immunoglobulin superfamily. (2006)
  • Author(s): Fujita E, Kouroku Y, Ozeki S, Tanabe Y, Toyama Y, Maekawa M, Kojima N, Senoo H, Toshimori K, Momoi T
  • Journal Title: Mol Cell Biol. 26・2 Pages: 718-726
• [Presentation] メタロプロテアーゼによるRA175/SynCAM1 (2008)
  • Author(s): 田辺裕子、笠原忠、桃井隆、藤田恵理子
  • Organizer: 特異的アイソフォームのプロセッシングNeuroscience 2008
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] メタロプロテアーゼによるRA175/SynCAM1特異的アイソフォームのプロセッシング (2008)
  • Author(s): 田辺裕子、笠原忠、桃井隆、藤田恵理子
  • Organizer: Neuroscience 2008
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-10
• [Presentation] The RA175/SynCAM complex on the GABAergic synapse (2007)
  • Author(s): Tanabe Y, Fujita E, Giulo P, Nigro V, Momoi MY, Ishiura S, Kasahara T, Momoi T
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] The RA175/SynCAM complex on the GABAergic synapse (2007)
  • Author(s): Y. Tanabe, E. Fujita, P. Giulo, V. Nigro, M. Y. Momai, S. lshiura, T. Kasahara, T Momoi
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2007-12-12
• [Presentation] 脳におけるRA175/SynCAM結合蛋白の解析 (2007)
  • Author(s): 田辺裕子、松崎鮎美、藤田恵理子、Giulo Piluso、大野茂男、石浦章一、Alaa Husseini、Vencenzo Nigro、桃井隆
  • Organizer: 第30回日本神経科学大会、第50回日本神経化学会大会、第17回日本神経回路学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-12
• [Presentation] 脳におけるRA175/SyaCAM結合蛋白の解析 (2007)
  • Author(s): 田辺 裕子、松崎 鮎美、藤田 恵理子、Giulo Pilus、大野 茂男、石浦 章一、Alaa Hussein、Vbnoenzo Mgm、桃井隆
  • Organizer: 30回日本神経科学大会、第50回日本神経化学会大会、第17回日本神経回路学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2007-09-12
• [Presentation] IGSF4A/ RA175 Interacts with PAR-3(IGSF4A/ RA175と結合する蛋白) (2006)
  • Author(s): Fujita E, Tanabe Y, Momoi T
  • Organizer: 第29回神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2006-07-09
• [Presentation] IGSF4A/ RA175 Interacts with PAR-3 and Its Deficiency Causes Defect in the Spermatocytes Differentiation. (2006)
  • Author(s): Fujita E, Tanabe Y, Senoo H, Toshimori K, Momoi T
  • Organizer: 第20回国際生化学・分子生物学会議(第79回日本生化学会大会/第29回日本分子生物学会年会)
  • Place of Presentation: 京都
  • Year and Date: 2006-06-20