Behavioral analysis of syntaxin knockout mice

• Project/Area Number: 18700368
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kyorin University
• Principal Investigator: FUJIWARA Tomonori Kyorin University, 医学部, 講師 (90255399)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: SNARE蛋白質 / マウス / 行動解析 / 開口放出 / シンタキシン / ノックアウトマウス / 異常行動

Research Abstract

中枢神経系のシナプス伝達において、シナプス小胞の開口放出が障害されることで、脳の高次機能にどのような影響を与えるか検討するため、開口放出を制御するSNARE蛋白質の欠損マウスの作成を計画し、それを用いた解析を行った。その結果これらのマウスで顕著な行動障害が見られ、ヒト精神神経疾患に関わる可能性が示唆された。

Research Products

• [Journal Article] Transient silencing of synaptic transmitter release from specific neuronal types by recombinant tetanus toxin light chain fused to antibody variable region. (2008)
  • Author(s): Kobayashi T, Kai N, Kobayashi K, Fujiwara T, Akagawa K, Onda M, Kobayashi K.
  • Journal Title: J Neurosci Methods 177 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Transient silencing of synaptic transmitter release from specific neuronal types by recombinant tetanus toxin light chain fused to antibody yari able region (2008)
  • Author(s): Kobayashi T, et.al.
  • Journal Title: J Neurosci Methods 177 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis. (2007)
  • Author(s): Ohara-Imaizumi M, Fujiwara T, Nakamichi Y, Okamura T, Akimoto Y, Kawai J, Matsushima S, Kawakami H, Watanabe T, Akagawa K, Nagamatsu S.
  • Journal Title: J Cell Biol 177(4) Pages: 695-705
  • Peer Reviewed
• [Journal Article] Enhancement of synaptic transmission and nociceptive behaviour in HPC-1/syntaxin 1A knockout mice following peripheral nerve injury. (2007)
  • Author(s): Takasusuki T. Fujiwara T., et al.
  • Journal Title: Eur. J. Neurosci. 26 Pages: 2179-87
  • Peer Reviewed
• [Journal Article] Imaging analysis reveals mechanistic differences between first- and se cond- phase insulin exocytosis. (2007)
  • Author(s): Ohara-Imaizumi M, Fujiwara T, et. al.
  • Journal Title: J Cell Biol. 177 Pages: 695-705
  • Peer Reviewed
• [Journal Article] Enhancement of synaptic transmission and nociceptive behaviour in HPC-l/suntaxin 1A knockout mice following peripheral nerve injury. (2007)
  • Author(s): Takasusuki T, Fujiwara T, et. al.
  • Journal Title: Eur. J. Neurosci. 26 Pages: 2179-87
  • Peer Reviewed
• [Journal Article] Essential role of Syntaxin 1A in first-phase insulin release and its implication in diabetes mellitus (2007)
  • Author(s): M.Ohara-Imaizumi et al.
  • Journal Title: J. Cell Biol. (In press)
• [Journal Article] Analysis of Knock-Out Mice to Determine the Role of HPC-1/Syntaxin 1A in Expressing Synaptic Plasticity (2006)
  • Author(s): Tomonori Fujiwara, Tatsuya Mishima, Takefumi Kofuji, Tomoki Chiba, Keiji Tanaka, Akitsugu Yamamoto, and Kimio Akagawa
  • Journal Title: The Journal of Neuroscience 26(21) Pages: 5767-5776
  • Peer Reviewed
• [Journal Article] Analysis of knockout mice to determine the role of HPC-1/syntaxin 1A in expressing synaptic plasticity (2006)
  • Author(s): T.Fujiwara, et al.
  • Journal Title: J. Neurosience 26 (21) Pages: 5767-5776
• [Presentation] Impairment of HPA-axis in HPC-1/syntaxinlA knockout mice (2008)
  • Author(s): Kofuji T, et.al.
  • Organizer: 神経化学会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] Effects of 5-HT signaling on cultured hippocampal neurons of HPC-1 /syntaxinl A knock-out mouse (2008)
  • Author(s): T. Fujiwara, et. al.
  • Organizer: 日本生理学会
  • Place of Presentation: 京王プラザ
  • Year and Date: 2008-03-26
• [Presentation] KOBAYASHI Functional analysis of the striatopallidal neural pathway by conditional transmission silencing (2008)
  • Author(s): T. KOBAYASHI, N. KAI, K. KOBAYASHI, T. FUJIWARA, K. AKAGAWA, M. ONDA, I. PASTAN, K.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington, DC.
• [Presentation] Masumi Sanada and Kimio Akagawa Impairment of HPA-axis in HPC-1/syntaxin1A knockout mice (2008)
  • Author(s): Takefumi Kofuji, Tomonori Fujiwara
  • Organizer: 日本神経化学会
  • Place of Presentation: 富山
• [Presentation] Functional analysis of the striatopallidal neural pathway by conditional transmission silencing (2008)
  • Author(s): T. KOBAYASHI, N. KAI, K. KOBAYASHI, T. FUJIWARA, K. AKAGAWA, M. ONDA, I. PASTAN, K. KOBAYASHI
  • Organizer: 神経科学会
  • Place of Presentation: 東京ビックサイト
• [Presentation] Effects of 5-HT signaling on cultured hippocampal neurons of HPC-1/syntaxin1A knock-out mouse (2008)
  • Author(s): Tomonori Fujiwara, Takefumi Kofuji, Tatsuya Mishima and Kimio Akagawa
  • Organizer: 日本生理学会
  • Place of Presentation: 京王プラザ
• [Presentation] A case of peripheral nervous system abnormality in HPC-1/syntaxin1A knockout mice (2008)
  • Author(s): Yuko Kaneko, Rie Suge, Tomonori Fujiwara, Kimio Akagawa and Shu-Ichi Watanabe
  • Organizer: 日本生理学会
  • Place of Presentation: 京王プラザ
• [Presentation] Functional analysis of the striatopallidal neural pathway by conditional transmission silencing (2008)
  • Author(s): Kobayashi T, et.al.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] Deletion of HPC-1/syntaxinlA gene causes autistic behavior in mice (2007)
  • Author(s): T. Fujiwara, et. al.
  • Organizer: 神経科学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-09-10
• [Presentation] Deletion of HPC-1/syntaxin1A gene causes autistic behavior in mice (2007)
  • Author(s): Tomonori Fujiwara, Takefumi Kofuji, Tatsuya Mishima, Takeo Yoshikawa and Kimio Akagawa
  • Organizer: 神経科学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Autistic-behavior in HPC-1/syntaxin1A knockout mice (2007)
  • Author(s): Tomonori Fujiwara, Rie Suge, Takefumi Kofuji, Tatsuya Mishima, Akiko Watanabe, Takeo Yoshikawa, Masahiko Nomura and Kimio Akagawa
  • Organizer: 日本生理学会
  • Place of Presentation: 大阪
• [Presentation] HPC-1/syntaxin1A knockout mice exhibit autistic-like behavior (2006)
  • Author(s): Takefumi Kofuji, Tomonori Fujiwara, Tatsuya Mishima, Akiko Watanabe, Takeo Yoshikawa and Kimio Akagawa
  • Organizer: 日本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-14
• [Presentation] Behavioral analysis of HPC-1/syntaxin1A knock-out mouse (2006)
  • Author(s): Tomonori Fujiwara, Tatsuya Mishima, Takefumi Kofuji and Kimio Akagawa
  • Organizer: 日本神経科学会
  • Place of Presentation: 京都