Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition.

• Project/Area Number: 18700606
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University of Medicine (2007-2008); University of Shizuoka (2006)
• Principal Investigator: ITO Tomoko Kyoto Prefectural University of Medicine, 医学部, 准教授(寄附講座) (80329648)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 糖尿病 / 感染症 / 活性酸素 / 殺菌 / 好中球 / スーパーオキシド / 貪食 / 抗酸化物質 / 合併症 / 消化器疾患 / グリケーション / プロテオミクス / 酸化ストレス

Research Abstract

ヒト急性骨髄性白血病細胞株由来の分化誘導後の好中球様細胞において高血糖状態によって活性酸素の過剰産生や貪食能の異常が認められた。糖尿病など高血糖によるストレス状態下での活性酸素産生能や殺菌作用の異常亢進は、傷害性因子が標的分子だけでなく周辺の組織へ傷害を生じ、重要臓器の機能障害や炎症性疾患を惹起する可能性が考えられた。また、各種茶粗抽出物や抗酸化物質が過剰に活性化された好中球様細胞に対して正常化効果を発揮し、炎症性疾患の防御に寄与するものと期待された。

Research Products

• [Journal Article] Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition (2008)
  • Author(s): Oya-Ito T, Naitou H, Masuda S, Kinae N, Ohashi N
  • Journal Title: Molecular Nutrition and Food Research 52 Pages: 360-369
  • Peer Reviewed
• [Journal Article] The other side of the Maillard reaction (2008)
  • Author(s): Nagaraj RH, Biswas A, Miller A, Oya-Ito T, Bhat M
  • Journal Title: Ann N Y Acad Sci 1126 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Functional analyses of neutrophil-like differentiated cell lines under a hvnorglveemic condition (2008)
  • Author(s): Oya-Ito, T.
  • Journal Title: Molecular Nutrition and Food Research 52 Pages: 360-369
  • Peer Reviewed
• [Journal Article] The other side of the Maillard reaction (2008)
  • Author(s): Nagaraj, R. H.
  • Journal Title: Ann N Y Acad Sci 1126 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Effect of methylglyoxal modification and phosphorylation on the chaperone and anti-apoptotic properties of heat shock protein 27 (2006)
  • Author(s): Oya-Ito T, Liu BF, Nagaraj RH
  • Journal Title: J Cell Biochem 99 Pages: 279-291
  • Peer Reviewed
• [Journal Article] Effect of site-directed mutagenesis of methylglyoxal-modifiable arginine residues on the structure and chaperone function of human alphaA-crystallin (2006)
  • Author(s): Biswas A, Miller A, Oya-Ito T, Santhoshkumar P, Bhat M, Nagaraj RH
  • Journal Title: Biochemistry 45 Pages: 4569-4577
  • Peer Reviewed
• [Journal Article] Detection of dideoxyosone intermediates of glycation using a monoclonal antibody : characterization of major epitope structures. (2006)
  • Author(s): Puttaiah S, Zhang Y, Pilch HA, Pfahler C, Oya-Ito T, Sayre LM, Nagaraj RH
  • Journal Title: Arch Biochem Biophys 446 Pages: 186-196
  • Peer Reviewed
• [Journal Article] 感染症を含む糖尿病合併症のバイオマーカー探索およびバイオマーカーを指標とした合併症予防因子探索 (2006)
  • Author(s): 伊藤友子 (大矢友子)
  • Journal Title: 静岡県立大学大学院生活健康科学研究科21世紀COEプログラム「先導的健康長寿学術研究推進拠点」研究報告集 Pages: 25-25
  • Peer Reviewed
• [Journal Article] 高血糖モデルにおける好中球様分化誘導細胞の機能解析 (2006)
  • Author(s): 伊藤(大矢)友子、内藤博敬、増田修一、大橋典男、木苗直秀
  • Journal Title: USフォーラム(静岡県立大学学術フォーラムV) Pages: 51-51
  • Peer Reviewed
• [Journal Article] Effect of site-directed mutagenesis of methylglyoxal-modifiable arginine residues on the structure and chaperone function of human alphaA-crystallin. (2006)
  • Author(s): Biswas, A., Miller, A., Oya-Ito, T., Santhoshkumar, P., Bhat, M., Nagaraj, R.H.
  • Journal Title: Biochemistry 45 Pages: 4569-4577
• [Journal Article] Detection of dideoxyosone intermediates of glycation using a monoclonal antibody : Characterization of major epitope structures. (2006)
  • Author(s): Puttaiah, S., Zhang, Y., Pilch, H.A., Pfahler, C., Oya-Ito, T., Sayre, L.M., Nagaraj, R.H.
  • Journal Title: Arch Biochem Biophys 446 Pages: 186-196
• [Journal Article] Effect of methylglyoxal modification and phosphorylation on the chaperone and anti-apoptotic properties of heat shock protein 27. (2006)
  • Author(s): Oya-Ito, T., Liu, B., Nagaraj, R.H.
  • Journal Title: J Cell Biochem 99 Pages: 279-291
• [Journal Article] 感染症を含む糖尿病合併症のバイオマーカー探索およびバイオマーカーを指標とした合併症予防因子探索 (2006)
  • Author(s): 伊藤 友子(大矢 友子)
  • Journal Title: 静岡県立大学大学院生活健康科学研究科21世紀COEプログラム「先導的健康長寿学術研究推進拠点」研究報告集 Pages: 25-25
• [Journal Article] 高血糖モデルにおける好中球様分化誘導細胞の機能解析 (2006)
  • Author(s): 伊藤(大矢)友子, 内藤 博敬, 増田 修一, 大橋 典男, 木苗 直秀
  • Journal Title: USフォーラム(静岡県立大学学術フォーラムV) Pages: 51-51
• [Presentation] Hsp27タンパク質のメチルグリオキザールによる翻訳後修飾の解析とその細胞生物学的意義 (2009)
  • Author(s): 大矢友子、内藤裕二、岡田ひとみ、濱野洋子、内山和彦、高木智久、半田修、八木信明、古倉聡、吉川敏一
  • Organizer: 第18回消化器とフリーラジカル研究会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-28
• [Presentation] Modification of Heat Shock Protein 27 in Intestinal Epithelium (2008)
  • Author(s): Oya-Ito, T., Naito, Y., Takagi, T., Handa, O., Fujiwake, H., Yamada, M., Shima, K., Yoshikawa, T. Methylglyoxal
  • Organizer: 18th Japan Maillard Reaction Symposium
  • Place of Presentation: Kawasaki
  • Year and Date: 2008-11-28
• [Presentation] Methylglyoxal Modification of Heat Shock Protein 27 in Intestinal Epithelium (2008)
  • Author(s): Oya-Ito, T.
  • Organizer: 18th Japan Maillard Reaction Symposium
  • Place of Presentation: Kawasaki
  • Year and Date: 2008-11-28
• [Presentation] 糖尿病における胃潰瘍治癒遅延に酸化修飾蛋白が関与する (2008)
  • Author(s): 平田育大、内藤裕二、高木智久、大矢友子、岡田ひとみ、鈴木隆裕、水島かつら、赤桐里美、尾松達司、岡山哲也、足立聡子、半田修、古倉聡、市川 寛、吉川敏一
  • Organizer: 消化管の炎症を考える会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-25
• [Presentation] Analysis of Acrolein Modification of Acetic Acid-induced Gastric Ulcer in Diabetic Mice (2008)
  • Author(s): Oya-Ito, T., Naito, Y., Takagi, T., Handa, O., Fujiwake, H., Yamada, M., Shima, K., Yoshikawa, T. Proteomic
  • Organizer: The International Symposium on Lipid Peroxidation 2008
  • Place of Presentation: Karuizawa
  • Year and Date: 2008-10-17
• [Presentation] Proteomic Analysis of Acrolein Modification of Acetic Acid-induced Gastric Ulcer in Diabetic Mice (2008)
  • Author(s): Oya-Ito, T.
  • Organizer: The International Symposium on Lipid Peroxidati on 2008
  • Place of Presentation: Karuizawa
  • Year and Date: 2008-10-16
• [Presentation] Identification of Hsp 27 as Methylglyoxal Target in Colon Mucosa of Uleorative Colitis (2008)
  • Author(s): Oya-Ito, T.
  • Organizer: 7th Annual World Congress Human Proteome Organisation (HUPO) 2008
  • Place of Presentation: Amsterdam
  • Year and Date: 2008-08-19
• [Presentation] Identification of Hsp 27 as Methylglyoxal Target in Colon Mucosa of Ulcerative Colitis (2008)
  • Author(s): Oya-Ito, T., Naito, Y., Takagi, T., Handa, O., Matsui, H., Yoshikawa, T
  • Organizer: 7th Annual World Congress Human Proteome Organisation (HUPO) 2008
  • Place of Presentation: Amsterdam
  • Year and Date: 2008-08-17
• [Presentation] 糖化反応生成物をマーカーとした消化器疾患のプロテオミクス解析 (2008)
  • Author(s): 大矢友子、内藤裕二、高木智久、半田修、松井裕史、吉川敏一
  • Organizer: 第61回日本酸化ストレス学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-19
• [Presentation] Identification of heat shock protein 27 (hsp27) as methylglyoxal target in colon mucosa of ulcerative colitis (2007)
  • Author(s): Oya-Ito, T., Naito, Y., Takagi, T., Handa, O., Matsui, H., Yoshikawa, T
  • Organizer: 17th Japan Maillard Meeting
  • Place of Presentation: Kobe
  • Year and Date: 2007-12-01
• [Presentation] Identification of heat shock protein 27(Hsp27) as methylglyoxal target in colon mucosa of ulcerative colitis (2007)
  • Author(s): Oya-Ito, T., Naito, Y., Takagi, T., Handa, O., Matsui, H., Yoshikawa, T.
  • Organizer: 17th Japan Maillard Meeting
  • Place of Presentation: Kobe,JAPAN
  • Year and Date: 2007-12-01
• [Presentation] Effect of methylglyoxal to neutrophil-like differentiated human myeloid leukemia cell lines (2007)
  • Author(s): Oya-Ito T., Naitou, H., Ohashi, N.,and Kinae, N
  • Organizer: 9th International Symposium on the Maillard Reaction
  • Place of Presentation: Munich
  • Year and Date: 2007-09-03
• [Presentation] Effect of methylglyoxal to neutrophil-like differentiated human myeloid leukemia cell lines. (2007)
  • Author(s): Oya-Ito T., Naitou, H., Ohashi, N., and Kinae, N.
  • Organizer: 9th International Symposium on the Maillard Reaction.
  • Place of Presentation: Munich,Germany
  • Year and Date: 2007-09-03
• [Presentation] 高濃度グルコース培養による好中球様分化誘導細胞の機能異常亢進作用と茶カテキン類を含む抗酸化物質による機能制御 (2007)
  • Author(s): 大矢友子、内藤博敬、増田修一、大橋典男、半田修、高木智久、内藤裕二、吉川敏一、木苗直秀
  • Organizer: 第4回日本カテキン学会総会
  • Place of Presentation: 静岡
  • Year and Date: 2007-08-23
• [Presentation] 高濃度グルコース培養による好中球様分化誘導細胞の機能異常充進作用と茶カテキン類を含む抗酸化物質による機能制御 (2007)
  • Author(s): 大矢 友子、内藤 博敬、増田 修一、大橋 典男、半田 修、高木 智久、内藤 裕二、吉川 敏一、木苗 直秀
  • Organizer: 第4回日本カテキン学会総会
  • Place of Presentation: 静岡
  • Year and Date: 2007-08-23
• [Presentation] 好中球様分化誘導THP-1の活性酸素産生に対するAGEsの影響 (2006)
  • Author(s): 木苗直秀、大村聖子、大矢友子、田家亜由美、内藤博敬、増田修一、永井竜児、大橋典男
  • Organizer: 第16回日本メイラード反応研究会
  • Place of Presentation: 東京
  • Year and Date: 2006-11-24
• [Presentation] メチルグリオキザールによるヒト好中球様分化誘導細胞の機能変化 (2006)
  • Author(s): 大矢友子、内藤博敬、増田修一、大橋典男、木苗直秀
  • Organizer: 第16回日本メイラード反応研究会
  • Place of Presentation: 東京
  • Year and Date: 2006-11-24
• [Presentation] Analysis of functional changes of neutrophil-like differentiated cell lines and effect of tea extracts on cell abnormality using a hyperglycemia model (2006)
  • Author(s): Oya-Ito, T., Naitou, H., Masuda, S., Ohashi, N., and Kinae, N
  • Organizer: The Kadota Fund International Forum 2006
  • Place of Presentation: Inuyama
  • Year and Date: 2006-11-21
• [Presentation] Functional changes of neutrophil-like differentiated human myeloid leukemia cell lines by carbonyl stress (2006)
  • Author(s): Oya-Ito, T., Naitou, H., Masuda, S., Kinae, N., and Ohashi, N
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-20