Development of a sensitive method for determination of fullerenes in biological samples and health risk assessment

• Project/Area Number: 18710059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Health Sciences
• Principal Investigator: KUBOTA Reiji National Institute of Health Sciences, 環境衛生化学部, 主任研究官 (80392299)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 微量化学物質汚染評価 / ナノマテリアル / フラーレン / 健康影響評価 / LC-MS / MS / 高感度分析法 / in vivo / ラット / 尾静脈投与 / マウス / 腹腔内投与 / 投与

Research Abstract

生体試料中フラーレン類の前処理法およびLC-MS/MSを用いた高感度測定法を開発した。in vivo系での複数の投与経路によるフラーレンの体内動態を調査し、経口投与による吸収は低く、尾静脈投与によって肝臓、肺、脾臓、腎臓に貯留すること、また経時的に蓄積量が減少することを明らかにした。また、尾静脈投与によるフラーレンの胎盤経由の母子間移行は少ない可能性があることを示した。

Research Products

• [Presentation] LC-MS/MSによるC60フラーレンへの経口、尾静脈投与による体内分布 (2009)
  • Author(s): 久保田領志, 田原麻衣子, 清水久美子, 杉本直樹, 西村哲治
  • Organizer: 日本薬理学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] LC-MS/MSによるC60フラーレンのラットへの経口、尾静脈投与による体内分布 (2009)
  • Author(s): 久保田領志、田原麻衣子、清水久美子、杉本直樹、西村哲治
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
• [Presentation] Tissue distribution of fullerene C60 in rat after oral and tail-vein administration by LC-MS/MS (2008)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K., Hirose A., Shimizu, K., Oku, N., and Nishimura, T.
  • Organizer: The 45^<th> Congress of the European Societies of Toxicology
  • Place of Presentation: Rhodes, Greece
  • Year and Date: 2008-10-07
• [Presentation] Tissue distribution of fullerene C60 in rat after oral and tail-vein administration by LC-MS/MS (2008)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K.., Hirose A., Shimizu, K., Oku, N., and Nishimura, T
  • Organizer: The 45th Congress of the European Societies of Toxicology
  • Place of Presentation: Rhodes, Greece
• [Presentation] Quantitative determination of C60 fullerene by LC-MS/MS and its tissue distribution following oral administration (2008)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K.., Tokunaga, H., Hirose A., Ema, M., and Nishimura, T
  • Organizer: 47th of the Society of Toxicology Annual Meeting
  • Place of Presentation: Seattle, USA
• [Presentation] Quantitative determination of C60 fullerene by LC-MS/MS and its tissue distribution following oral administration. (2008)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K., Tokunaga, H., Hirose A., Ema, M., and Nishimura, T.
  • Organizer: 47th of the Society of Toxicology Annual Meeting
  • Place of Presentation: Seattle, USA
• [Presentation] Tissue distribution of C60 fullerene in mice after intraperitoneal administration by liquid chromatography-tandem massspectrometry (2006)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K.., Tokunaga, H., Hirose A., and Nishimura, T
  • Organizer: 27th Annual Meeting of American College of Toxicology
  • Place of Presentation: Indian Wells, USA
• [Presentation] Tissue distribution of C60 fullerene in micea after intraperitoneal administration by liquid chromatography-tandem mass spectrometry. (2006)
  • Author(s): Kubota, R., Tahara, M., Shimizu, K., Tokunaga, H., Hirose A., and Nishimura, T.
  • Organizer: 27th Annual Meeting of American College of Toxicology
  • Place of Presentation: Indian Wells, USA