Identification of a cell death inducible factor in autophagy-defective plants

• Project/Area Number: 18770040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: The Institute of Physical and Chemical Research (2007-2008); National Institute for Basic Biology (2006)
• Principal Investigator: YOSHIMOTO Koki The Institute of Physical and Chemical Research, 植物免疫研究チーム, 基礎科学特別研究員 (40399316)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: オートファジー / 液胞 / オルガネラ / 細胞死 / 老化 / タンパク質分解 / サリチル酸

Research Abstract

オートファジーは,細胞内タンパク質を液胞に送り込み,分解する現象であり,液胞分解機構の中心的な役割を担っている.申請者は,オートファジー不能植物(ATG遺伝子破壊株)の表現型解析から,高等植物ではタンパク質のリサイクリング・アミノ酸の供給源としてのオートファジーの生理的役割に加え,細胞死において植物ならではの役割が存在することを明らかにした.

Research Products

• [Journal Article] Autophagy plays a role in chloroplast degradation during leaf senescence in individually darkened leaves (2009)
  • Author(s): Wada, S., Ishida, H., Izumi, M., Yoshimoto, K., Ohsumi, Y., Mae, T., Makino, A.
  • Journal Title: Plant Physiol. 149 Pages: 885-893
  • Peer Reviewed
• [Journal Article] OsATG10b, an Autophagosome component, is needed for cell survival against oxidative stresses in rice (2009)
  • Author(s): Shin, J. H., Yoshimoto, K., Ohsumi, Y., Jeon, J. S., An, G.
  • Journal Title: Mol. Cells 27 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy (2008)
  • Author(s): Ishida, H., Yoshimoto, K.
  • Journal Title: Autophagy 4 Pages: 961-962
  • Peer Reviewed
• [Journal Article] Mobilization of Rubisco and stromal-localized fluorescent proteins of chloroplasts to the vacuole by ATG gene-dependent autophagic process (2008)
  • Author(s): Ishida, H., Yoshimoto, K., Izumi, M., Reisen, D., Yano, Y., Makino, A., Ohsumi, Y., Hanson, M. R., Mae, T.
  • Journal Title: Plant Physiol. 148 Pages: 142-155
  • Peer Reviewed
• [Journal Article] In vitro reconstitution of plant ATG8 and ATG12 conjugation systems essential for plant autophagy (2008)
  • Author(s): Fujioka, Y., Noda, N. N., Fujii, K., Yoshimoto, K., Ohsumi, Y., Inagaki, F.
  • Journal Title: J. Biol. Chem. 283 Pages: 1921-1928
  • Peer Reviewed
• [Journal Article] Mobilization of Roubisco and stromal-localized fluorescent proteins of chloroplasts to the vacuole by ATG gene-dependent autophagic process. (2008)
  • Author(s): Ishida, H., et al.
  • Journal Title: Plant Physiol 148 Pages: 142-155
  • Peer Reviewed
• [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy. (2008)
  • Author(s): Ishida, H., et al.
  • Journal Title: Autophagy 4 Pages: 961-962
  • Peer Reviewed
• [Journal Article] In vitro reconstitution of plant ATG8 and ATG12 conjugation systems essential for plant autophagy. (2008)
  • Author(s): Fujioka, Y., et. al.
  • Journal Title: J. Biol. Chem. 283 Pages: 1921-1928
  • Peer Reviewed
• [Journal Article] An Arabidopsis homolog of yeast ATG6/VPS30 is essential for pollen germination (2007)
  • Author(s): Fujiki, Y., Yoshimoto, K., Ohsumi, Y.
  • Journal Title: Plant Physiol. 143 Pages: 1132-1139
  • Peer Reviewed
• [Journal Article] An Arabidopsis homolog of yeast ATG6/VPS30 is essential for pollen germination. (2007)
  • Author(s): Fujiki, Y. et al.
  • Journal Title: Plant Physiol. 143 Pages: 1132-1139
• [Journal Article] AtATG genes, homologs of yeast autophagy genes, are involved in constitutive autophagy in Arabidopsis root tip cells (2006)
  • Author(s): Inoue, Y, Suzuki, T, Hattori, M, Yoshimoto, K., Ohsumi, Y, Moriyasu, Y.
  • Journal Title: Plant Cell Physiol. 47 Pages: 1641-1652
  • Peer Reviewed
• [Journal Article] 高等植物のオートファジー (2006)
  • Author(s): 吉本光希
  • Journal Title: 顕微鏡 41 Pages: 84-86
  • NAID: 10018183079
• [Journal Article] 植物オートファジー研究の新展開 : 植物のatg変異体 (2006)
  • Author(s): 吉本光希
  • Journal Title: 蛋白質核酸酵素 増刊号 ユビキチン-プロテアソーム系とオートファジー 51 Pages: 1532-1536
• [Journal Article] AtATG genes, homologs of yeast autophagy genes, are involved in constitutive autophagy in Arabidopsis root tip cells. (2006)
  • Author(s): Inoue, Y. et al.
  • Journal Title: Plant Cell Physiol. 47 Pages: 1641-1652
• [Journal Article] 高等植物のオートファジー (2006)
  • Author(s): 吉本 光希
  • Journal Title: 顕微鏡 41 (2) Pages: 84-86
  • NAID: 10018183079
• [Journal Article] 植物オートファジー研究の新展開 : 植物のatg変異体 (2006)
  • Author(s): 吉本 光希
  • Journal Title: 蛋白質核酸酵素 51 (10) Pages: 1532-1536
• [Journal Article] Autophagy plays a role in chloroplast degradation during leaf senescence in individually darkened leaves.
  • Author(s): Wada, S., et al.
  • Journal Title: Plant Physiol (in press)
  • Peer Reviewed
• [Presentation] 植物オートファジーの新たな生理的役割 (2009)
  • Author(s): 吉本光希, 大隅良典, 白須賢
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-23
• [Presentation] 植物オートファジーの新たな生理的役割 (2009)
  • Author(s): 吉本光希, et al.
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-23
• [Presentation] 植物オートファジーと細胞死の制御 (2008)
  • Author(s): 吉本光希, 白須賢, 大隅良典
  • Organizer: シンポジウム 植物の生体防御と細胞死,日本分子生物学会年会・日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 植物のオートファジーと細胞死の制御 (2008)
  • Author(s): 吉本光希, et al.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Plant autophagy negatively regulates senescence by eliminating salicylic acid signaling (2008)
  • Author(s): Yoshimoto, K. Shirasu, K., Ohsumi, Y.
  • Organizer: Gordon Research Conference, Plant Senescence
  • Place of Presentation: Massachusetts, USA
• [Presentation] Plant autophagy negatively regurates senescense by eliminating salicylic acid signaling (2008)
  • Author(s): Yoshimoto, K., et al.
  • Organizer: Gordon Research Conference, Plant Senescence
  • Place of Presentation: Masachusetts, USA