Studies on the molecular mechanism of Hsp110, a key component in the protein folding machinery

• Project/Area Number: 18770094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: University of Hyogo
• Principal Investigator: SHOMURA Yasuhito University of Hyogo, 大学院生命理学研究科, 助教 (50423900)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,220,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥120,000); Fiscal Year 2008: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: Hsp110 / 分子シャペロン / X線結晶構造解析 / フォールディング / Sse1p / Hspl10 / Sselp

Research Abstract

タンパク質は多数のアミノ酸が繋がってできた直鎖状の高分子であり,正しく折りたたまれることによって機能を獲得する.本研究では,タンパク質の折りたたみを介助する因子として最近同定されたHsp110の分子機構の解明を試みた.その結果,Hsp110は,折りたたみに利用されるHsp70によるATPの加水分解反応を促進する因子の一つであることが明らかとなり,その立体構造の決定によって作用機序のモデルが得られた

Research Products

• [Journal Article] (2006)Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone (2006)
  • Author(s): Dragovic Z., Shomura Y., Tzvetkov N., Hartl F.U., Bracher A
  • Journal Title: Ssb1p. Biol. Chem 387 Pages: 1593-1600
• [Journal Article] Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s (2006)
  • Author(s): Dragovic, Z., Broadley, S. A., Shomura, Y., Bracher, A. & Hartl, F. U.
  • Journal Title: EMBO J 25 Pages: 2519-28
• [Journal Article] Feslp acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssblp. (2006)
  • Author(s): Dragovic Z.
  • Journal Title: Biological Chemistry 387 Pages: 1593-1600
• [Journal Article] Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s. (2006)
  • Author(s): Dragovic Z.
  • Journal Title: EMBO Journal 25 Pages: 2519-2528
• [Presentation] Crystal structure of the full-length Hsp110 molecular chaperone from Schizosaccharomyces pombe in the nucleotide-free state (2009)
  • Author(s): Shomura Y., Bracher A., Hartl F.U. and Higuchi Y
  • Organizer: XXI Congress of the International Union of Crystallography Congress and General Assembly, poster presentation
  • Place of Presentation: Osaka
• [Presentation] Crystal structure of the full-length Hsp110 molecular chaperone from Schizosaccharomyces pombe in the nucleotide-free state (2008)
  • Author(s): Shomura Y
  • Organizer: XXI Congress and General Assembly of the Inter national Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-26