Molecular structure and function of water oxidation in Photosystem II : for application to hydrogen production

• Project/Area Number: 18770116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Ehime University (2008); Osaka Prefecture University (2006-2007)
• Principal Investigator: SUGIURA Miwa Ehime University, 無細胞生命科学工学研究センター, 准教授 (80312255)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,950,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: 生体エネルギー変換 / 光合成電子伝達系 / 酸素発生 / 部位特異的変異 / 好熱性ラン藻 / 光化学系II / 好熱性シアノバクテリア / 水素エネルギー

Research Abstract

光合成によるエネルギー変換機能を改良して新規エネルギー生産に応用することを目的として、その初発の反応である水の酸化機能を高めた組換え体の作製を試みた。遺伝子組換えによって遺伝子の発現を制御したり、光化学系II反応中心のアミノ酸を変えることにより、水の酸化機能を1.8倍高めることに成功した。更に機能を高める目的で、水の酸化で生じた電子を受け取る+1.2 Vの高い酸化還元電位を持つP680クロロフィルのリガンドアミノ酸を別のアミノ酸に置換したところ、水の酸化には殆ど影響しないことが明らかになった。

Research Products

• [Journal Article] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II (2009)
  • Author(s): Boussac, A., Sugiura, M., Rutherford, A.W. and Dorlet, P.
  • Journal Title: J. Am. Chem. Soc 131 Pages: 5050-5051
• [Journal Article] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine (2009)
  • Author(s): Rappaport, F. Boussac, A., Force, D. A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R. and Diner, B.
  • Journal Title: J. Am. Chem. Soc 131 Pages: 4425-4433
• [Journal Article] 酸素発生型光合成微生物による光-水素生成、新エネルギーの開発の現状と将来特集 (2009)
  • Author(s): 杉浦美羽
  • Journal Title: KEC情報(関西電子工業振興センター発刊) 208 Pages: 28-31
• [Journal Article] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine (2009)
  • Author(s): IRappaport, F., Boussac, A., Force, D. A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R., and Diner. B
  • Journal Title: J. Am. Chem. Soc 131 Pages: 4425-4433
  • Peer Reviewed
• [Journal Article] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II (2009)
  • Author(s): Boussac, A., Sugiura, M., Rutherford, A. W., and Dorlet, P
  • Journal Title: J. Am. Chem. Soc 131 Pages: 5050-5051
  • Peer Reviewed
• [Journal Article] Monitoring proton release during photosynthetic water oxidation in photosystem II by means of isotope-edited infrared spectroscopy (2009)
  • Author(s): Suzuki, H., Sugiura, M. and Noguchi, T
  • Journal Title: J. Am. Chem. Soc (In press)
  • Peer Reviewed
• [Journal Article] 酸素発生型光合成微生物による光-水素生成 (2009)
  • Author(s): 杉浦美羽
  • Journal Title: KEC情報(関西電子工業振興センター発刊) 208 Pages: 28-31
• [Journal Article] The role of TyrD in the electron transfer kinetics in Photosystem II (2008)
  • Author(s): Szczepaniak, M., Sugiura, M. and Holzwarth, A.R.
  • Journal Title: Biochim. Biophys. Acta (Bioenergetics) 1777 Pages: 1510-1517
• [Journal Article] Monitoring water reactions during the S-state cycle of the photosynthetic water-oxidizing center : Detection of the DOD bending vibrations by means of Fourier transform infrared spectroscopy (2008)
  • Author(s): Suzuki, H., Sugiura, M. and Noguchi, T.
  • Journal Title: Biochemistry 47 Pages: 22024-11030
• [Journal Article] Analysis of xenon binding to photosystem Ii by X-ray crystallography (2008)
  • Author(s): Murray, J.W., Maghlaoui, K., Kargul, J., Sugiura, M., and Barber, J.
  • Journal Title: Photosynth Res 98 Pages: 523-527
• [Journal Article] Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803 (2008)
  • Author(s): Inoue-Kashino, N., Takahashi, T., Ban, A., Sugiura, M., Takahashi, Y., Satoh, K. and Kashino, Y
  • Journal Title: Photosynth. Res 98 Pages: 323-335
• [Journal Article] Quantum efficiency distributions of photo-induced side-pathway donor oxidation at cryogenic temperature in Photosystem II (2008)
  • Author(s): Hughes, J. L., Rutherford, A. W., Sugiura, M. and Krausz, E.
  • Journal Title: Photosynth. Res 98 Pages: 199-266
• [Journal Article] X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II (2008)
  • Author(s): Murray, J.W, Maghlaoui, K., Kargul, J., Ishida, N., Lai, T.-L., Rutherford, A.W., Sugiura, M., Boussac. A. and Barber, J.
  • Journal Title: Energy & Environmental Science 1 Pages: 161-166
• [Journal Article] Isotopic labelling of Photosystem II in Thermosynechococcus elongatus (2008)
  • Author(s): Boussac, A., Verbavatzand, J.-M., and Sugiura, M.
  • Journal Title: Photosynth. Res 98 Pages: 285-292
• [Journal Article] Biosynthetic Exchange of Bromide for Chloride and Strontium for Calcium in the Photosystem II Oxygen-evolving Enzymes (2008)
  • Author(s): Ishida, N, Sugiura, M., Rappaport, F., Lai, T.-L., Rutherford, A.W., and Boussac, A.
  • Journal Title: J. Biol. Chem 283 Pages: 13330-13340
• [Journal Article] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mn cluster in Photosystem II (2008)
  • Author(s): Aoyama, C., Suzuki, H., Sugiura, M. and Noguchi, T.
  • Journal Title: Biochemistry 47 Pages: 2760-2765
• [Journal Article] Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II (2008)
  • Author(s): Sugiura, M., Boussac, A., Noguchi, T. and Rappaport, F.
  • Journal Title: Thermosynechococcus elongatus Biochim. Biophys. Acta (Bioenergetics) 1777 Pages: 331-342
• [Journal Article] Low temperature illuminations of Photosystem II from Thermosynechococcus elongatus by visible and near-infrared lights (2008)
  • Author(s): Boussac, A., Sugiura, M., Lai, T.-L. and Rutherford, A.W.
  • Journal Title: Philos. Trans. R. Soc. Lond.: B. Biol. Sci. 363 Pages: 1203-1210
• [Journal Article] Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P_<680>, of Photosystem II Thermosynechococcus elongatus (2008)
  • Author(s): Sugiura, M., Boussac, A., Noguchi, T. and Rappaport, F
  • Journal Title: Biochim. Biophys. Acta (Bioenergetics) 1777 Pages: 331-342
  • Peer Reviewed
• [Journal Article] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mn cluster in Photosystem II (2008)
  • Author(s): Aoyama, C., Suzuki, H., Sugiura, M. and Noguchi, T
  • Journal Title: Biochemistry 47 Pages: 2760-2765
  • Peer Reviewed
• [Journal Article] Biosynthetic Exchange of Bromide for Chloride and Strontium for Calcium in the Photosystem II Oxygen-evolving Enzymes (2008)
  • Author(s): Ishida, N, Sugiura, M., Rappaport, F., Lai, T.-L., Rutherford, A.W., and Boussac, A
  • Journal Title: J. Biol. Chem. 283 Pages: 13330-13340
  • Peer Reviewed
• [Journal Article] Isotopic labelling of Photosystem II in Thermosynechococcus elongatus (2008)
  • Author(s): Boussac, A., Verbavatzand, J.-M., and Sugiura, M
  • Journal Title: Photosynth. Res 98 Pages: 285-292
  • Peer Reviewed
• [Journal Article] X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II (2008)
  • Author(s): Murray, J.W, Maghlaoui, K., Kargul, J., Ishida, N., Lai, T.-L., Rutherford, A.W., Sugiura, M., Boussac. A. and Barber, J
  • Journal Title: Energy & Environmental Science 1 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Quantum efficiency distributions of photo-induced side-pathway donor oxidation at cryogenic temperature in Photosystem II (2008)
  • Author(s): Hughes, J. L., Rutherford, A. W., Sugiura, M. and Krausz, E
  • Journal Title: Photosynth. Res 98 Pages: 199-266
  • Peer Reviewed
• [Journal Article] Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803 (2008)
  • Author(s): Inoue-Kashino, N., Takahashi, T., Ban, A., Sugiura, M., Takahashi, Y., Satoh, K., and Kashino, Y
  • Journal Title: Photosynth. Res 98 Pages: 323-335
  • Peer Reviewed
• [Journal Article] Monitoring water reactions during the S-state cycle of the photosynthetic water-oxidizing center: Detection of the DOD bending vibrations by means of Fourier transform infrared spectroscopy (2008)
  • Author(s): Suzuki, H., Sugiura, M. and Noguchi, T
  • Journal Title: Biochemistry 47 Pages: 22024-11030
  • Peer Reviewed
• [Journal Article] The role of TyrD in the electron transfer kinetics in Photosystem II (2008)
  • Author(s): Szczepaniak, M., Sugiura, M., and Holzwarth, A. R
  • Journal Title: Biochim. Biophys. Acta (Bioenergetics) 1777 Pages: 1510-1517
  • Peer Reviewed
• [Journal Article] Low temperature illuminations of Photosystem II from Thermosynechococcus elongatus by visible and near-infrared lights (2008)
  • Author(s): Boussac, A., Sugiura, M., Lai, T.-L.and Rutherford, A.W.
  • Journal Title: Philosophical Transactions of the Royal Society:B 363 Pages: 1203-1210
  • Peer Reviewed
• [Journal Article] Influence of Histidine-198 of the Dl subunit on the properties of the primary lectron donor,P_680,of Photosystem II Thermosynechococcus elongatus (2008)
  • Author(s): Sugiura, M., Boussac, A., Noguchi, T.and Rappaport, F.
  • Journal Title: Biochim.Biophys.Acta(Bioenergetics) 1778 Pages: 331-342
  • Peer Reviewed
• [Journal Article] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mncluster in Photosystem II (2008)
  • Author(s): Aoyama, C., Suzuki, H., Sugiura, M.and Noguchi, T.
  • Journal Title: Biochemistry 47 Pages: 2760-2765
  • Peer Reviewed
• [Journal Article] Water molecules coupled to the redox-active tyrosine YD in Photosystem II as detection by FTIR Spectroscopy (2007)
  • Author(s): Takahashi, R., Sugiura, M. and Noguchi, T.
  • Journal Title: Biochemistry 46 Pages: 14245-14249
• [Journal Article] Ycf12 is a core subunit in photosystem II complex (2007)
  • Author(s): Kashino, Y., Ikeda, Y., Ban, A., Inoue-Kashino, N., Satoh, K. and Sugiura, M.
  • Journal Title: Biochim. Biophys. Acta (Bioenergetics) 1767 Pages: 1269-1275
• [Journal Article] A nitrite transporter associated with nitrite uptake by higher plant chloroplasts (2007)
  • Author(s): Sugiura, M, Georgescu, M. N. and Takahashi, M.
  • Journal Title: Plant Cell Physiol 48 Pages: 1022-1035
  • NAID: 10027003214
• [Journal Article] Perturbation of the structure of P680 and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by Fourier transform infrared spectroscopy (2007)
  • Author(s): Okubo, T., Sugiura, M., Tomo, T., and Noguchi, T.
  • Journal Title: Biochemistry 46 Pages: 4390-4397
• [Journal Article] Characterization of the tyrosine-Z radical and its environment in the spin-coupled S2TyrZ state of Photosystem II from Thermosynechococcus elongatus (2007)
  • Author(s): Un, S., Boussac, A. and Sugiura, M.
  • Journal Title: Biochemistry 46 Pages: 3138-3150
• [Journal Article] A nitrite transporter associated with nitrite uptake by higher plant chloroplasts (2007)
  • Author(s): Sugiura, M, Georgescu, M.N.and Takahashi, M.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1022-1035
  • NAID: 10027003214
  • Peer Reviewed
• [Journal Article] Ycfl2 is a core subunit in photosystem II complex (2007)
  • Author(s): Kashino, Y., Ikeda, Y., Ban, A., Inoue-Kashino, N., Satoh, K.and Sugiura, M.
  • Journal Title: Biochim.Biophys.Acta(Bioenergetics) 1767 Pages: 1269-1275
  • Peer Reviewed
• [Journal Article] Water molecules coupled to the redox-active tyrosine Y_D in Photosystem II as detection by FTIR Spectroscopy (2007)
  • Author(s): Takahashi, R., Sugiura, M.and Noguchi, T.
  • Journal Title: Biochemistry 46 Pages: 14245-14249
  • Peer Reviewed
• [Journal Article] Characterization of the Tyrosine-Z radical and Its Environment in the Spin-Coupled S_2Tyr_z State of Photosystem II from Thermosynechococcus elongatus (2007)
  • Author(s): Sun Un, Alain Boussac, Miwa Sugiura
  • Journal Title: Biochemistry 46 Pages: 3138-3150
• [Journal Article] Perturbation of the structure of P_<680> and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by Fourier transform infrared spectroscopy (2007)
  • Author(s): Okubo, T., Sugiura, M., Tomo, T., Noguchi, T.
  • Journal Title: Biochemistry 46(印刷中)
• [Journal Article] Structural perturbation of the carboxylate ligands to the Mn cluster upon Ca2+/Sr2+ exchange in the S-state cycle of photosynthetic oxygen evolution as studied by flash-induced FTIR difference spectroscopy (2006)
  • Author(s): Suzuki, H., Taguchi, Y., Sugiura, M., Boussac, A. and Noguchi, T.
  • Journal Title: Biochemistry 45 Pages: 13454-13464
• [Journal Article] Structural Perturbation of the Carboxylate Ligands to the Mn Cluster upon Ca^<2+>/Sr^<2+> Exchange in the S-state Cycle of Photosynthetic Oxygen (2006)
  • Author(s): Suzuki, H., Taguchi, Y., Sugiura, M., Boussac, A., Noguchi, T
  • Journal Title: Biochemistry 45 Pages: 13454-13464
• [Presentation] 光化学系IIタンパク質複合体の分子構造の解析の現状 (2009)
  • Author(s): 杉浦美羽
  • Organizer: 平成20年度愛媛地区分析化学講演会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2009-01-29
• [Presentation] 水の酸化反応におけるD1-His332の役割 (2009)
  • Author(s): 杉浦美羽、大野陽平、Alain Boussac、Fabrice Rappaport、鈴木博行、野口巧、林秀則
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 酸化反応におけるDl-His332の役割 (2009)
  • Author(s): 杉浦美羽、大野陽平、Alain Boussac、Fabrice Rappaport、鈴木博行、野口巧、林秀則
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光化学系IIにおける水の分解メカニズム (2008)
  • Author(s): 杉浦美羽
  • Organizer: 2008年度日本化学会中国四国支部会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2008-12-04
• [Presentation] 光化学系IIにおける水の分解メカニズム (2008)
  • Author(s): 杉浦美羽
  • Organizer: 日本化学会中国四国支部会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2008-12-04
• [Presentation] Effects of P680 ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus (2008)
  • Author(s): Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport
  • Organizer: Protein Island Matsuyama Inernational Symposium
  • Place of Presentation: Matsuyama
  • Year and Date: 2008-09-26
• [Presentation] Effects of P_<680> ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus (2008)
  • Author(s): Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport
  • Organizer: Protein Island Matsuyama Inernational Symposium
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2008-09-26
• [Presentation] 酸素発生型光合成微生物による光-水素生成 (2008)
  • Author(s): 杉浦美羽
  • Organizer: 2008年度KECセミナー新エネルギー開発の現状と将来-更なるCO2削減を目指して-
  • Place of Presentation: (社)関西電子工業振興センター
  • Year and Date: 2008-07-18
• [Presentation] 酸素発生型光合成微生物による光-水素生成 (2008)
  • Author(s): 杉浦美羽
  • Organizer: 2008年度KECセミナー新エネルギー開発の現状と将来〜更なるCO_2削減を目指して〜
  • Place of Presentation: (社)関西電子工業振興センター
  • Year and Date: 2008-07-18
• [Presentation] The water splitting enzyme: recent advances (2008)
  • Author(s): A., Boussac, N., Ishida, T.-L., Lai, A.W,. Rutherford, F., Rappaport, Y., Pushkar, M., Sugiura
  • Organizer: 第46回日本生物物理学会シンポジウム-光合成の新たなる潮流-
  • Place of Presentation: 福岡国際会議場
• [Presentation] 光化学系IIの構造と機能の解明から応用まで (2008)
  • Author(s): 杉浦美羽
  • Organizer: 第46回日本生物物理学会シンポジウム-光合成の新たなる潮流-
  • Place of Presentation: 福岡国際会議場
• [Presentation] 光化学系IIの構造と機能の解明から応用まで (2008)
  • Author(s): 杉浦美羽
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Effects of P_680 ligand in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus (2007)
  • Author(s): Miwa Sugiura
  • Organizer: The 14th International Congress on Photosynthesis
  • Place of Presentation: Glasgow(Scotland)
  • Year and Date: 2007-07-23
• [Presentation] Spectroscopic studies of the Mn_4Ca cluster in mutans and biosyntheticallt modified Photosystem II from Thermosynechococcus elongatus (2007)
  • Author(s): Alain Boussaz, Miwa Sugiura, Naoko Ishida, Sun Un, Fabrice Rappaport, and A.William Rutherford
  • Organizer: The 14th International Congress on photosynthesis
  • Place of Presentation: Glasgow(Scotland)
  • Year and Date: 2007-07-23
• [Presentation] FTIR study on the proton release pattern duing water oxidation in Photosystem II core complexes from Thermosynechococcus elongatus (2007)
  • Author(s): Hiroyuki Suzuki, Miwa Sugiura, and Takumi Noguchi
  • Organizer: The 14th International Congress on photosynthesi
  • Place of Presentation: Glasgow(Scotland)
  • Year and Date: 2007-07-23
• [Presentation] Structural coupling of water molecules with Y_D in Photosystem II as revealed by FTIR spectroscopy (2007)
  • Author(s): Ryota Takahashi, Miwa Sugiura, and Takumi Noguchi
  • Organizer: The 14th International Congress on Photosynthesis
  • Place of Presentation: Glasgow(Scotland)
  • Year and Date: 2007-07-23
• [Presentation] Influence of the redox active Tyrosine D on the electron transfer in cyanobacterial Photosytem II (2007)
  • Author(s): Malwina Szczepaniak, Miwa Sugiura, and Alfred R.holzwarth
  • Organizer: The 14th International Congress on Photosynthesis:Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Dryme(Scotland)
  • Year and Date: 2007-07-12
• [Presentation] 光化学系IIにおける酸素発生の分子機能と構造:部位特異的変異を導入した好熱性シアノバクテリアが解明のブレークスルーになる (2007)
  • Author(s): 杉浦美羽
  • Organizer: 第87回日本化学会春期年会特別企画シンポジウム(光合成機能の分子メカニズムと工学応用-分子レベルの探求から太陽光エネルギー変換系への応用まで-)
  • Place of Presentation: 関西大学
  • Year and Date: 2007-03-26
• [Presentation] Thermosynechococcus elongatus光化学系IIにおけるP680リガンドの酸化還元電位への影響 (2007)
  • Author(s): 杉浦美羽、Alain Boussac、野口巧、Fabrice Rappaport
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 愛媛大学
• [Presentation] Spectroscopic studies of the Mn_4Ca cluster in mutants and biosynthetically modified photosystem II from Thermosynechococcus elongatus (2007)
  • Author(s): Alain Boussac and Miwa Sugiura
  • Organizer: Royal Society meeting: How Nature uses sulight to split water
  • Place of Presentation: Royal Society(London)
• [Presentation] P_680ligand in the oxyen-evolving Photosystem II of Thermosynechococcus elongatus (2007)
  • Author(s): Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport
  • Organizer: The 14th International Congress on photosynthesis:Satellite Workshop on Solar Energy and Artificial Photosynthesis
  • Place of Presentation: Royal Society (London)
• [Patent(Industrial Property Rights)] 光合成を利用した酸素発生型光合成微生物による水素生産方法 (2008)
  • Inventor(s): 杉浦美羽
  • Industrial Property Rights Holder: 杉浦美羽
  • Industrial Property Number: 2008-006011
  • Filing Date: 2008-01-15
• [Patent(Industrial Property Rights)] 光合成を利用した酸素発生型光合成微生物による水素生産方法 (2008)
  • Inventor(s): 杉浦 美羽
  • Industrial Property Rights Holder: 大阪府立大学
  • Industrial Property Number: 2008-006011
  • Filing Date: 2008-01-15