Ion transporting mechanism of V-type Na+-ATPase from Enterococcus hirae

• Project/Area Number: 18770122
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kyoto University (2007-2008); The Institute of Physical and Chemical Research (2006)
• Principal Investigator: MURATA Takeshi Kyoto University, 大学院医学研究科, 助教 (80415322)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: V-ATPase / X線結晶構造解析 / ポンプ / ナトリウム / モーター / 複合体 / 膜タンパク質 / Enterococcus hirae

Research Abstract

研究代表者らは腸内連鎖球菌に存在するNa+輸送性V 型ATPase の分子生物学的・生化学的・構造生物学的研究を展開し、膜タンパク質部分であるNtpKリングのX線結晶構造解析に成功していた。本研究では本酵素のイオン輸送機構の解明を目的に、Li+結合型及び阻害剤DCCD結合型のNtpKリングのX線結晶構造を明らかにした。これらの構造と生化学的解析から、本酵素のイオン結合/解離のメカニズムが明らかになった。

Research Products

• [Journal Article] Ion binding and selectivity of the rotor ring of the Na+-transporting V-ATPase (2008)
  • Author(s): Takeshi Murata, Ichiro Yamato, Yoshimi Kakinuma, Mikako Shirouzu, John E. Walker, Shigeyuki Yokoyama, and So Iwata
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A 105 Pages: 8607-8612
  • Peer Reviewed
• [Journal Article] Interaction and Stoichiometry of the Peripheral Stalk Subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI, of Enterococcus hirae V-ATPase (2008)
  • Author(s): Misaki Yamamoto, Satoru Unzai, Shinya Saijo, Kazuki Ito, Kenji Mizutani, Chiyo Suno-Ikeda, Yukako Yabuki-Miyata, Takaho Terada, Mitsutoshi Toyama, Mikako Shirouzu, Takuya Kobayashi, Yoshimi Kakinuma, Ichiro Yamato, Shigeyuki Yokoyama, So Iwata, and Takeshi Murata
  • Journal Title: J. Biol. Chem 283 Pages: 19422-19431
  • Peer Reviewed
• [Journal Article] E stimation of ligand binding free energies of F-ATPase by using molecular dynamics/free energy calculation (2008)
  • Author(s): Atsushi Suenaga, Osamu Umezu, Tadashi Ando, Ichiro Yamato, Takeshi Murata, Makoto Taiji
  • Journal Title: J. Comput. Chem. Jpn 7 Pages: 103-116
  • Peer Reviewed
• [Journal Article] Ion binding and selectivity of the rotor ring of the Na+-transporting V-ATPase (2008)
  • Author(s): Takeshi Murata
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A 105 Pages: 8607-8612
  • Peer Reviewed
• [Journal Article] Interaction and Stoichiometry of the Peripheral Stalk Subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI, of Enterococcus hirae V-ATPase (2008)
  • Author(s): Misaki Yamamoto
  • Journal Title: J. Biol. Chem 283 Pages: 19422-19431
  • Peer Reviewed
• [Journal Article] Estimation of ligand binding free energies of F-ATPaseby using molecular dynamics/free energy calculation (2008)
  • Author(s): Atsushi Suenaka
  • Journal Title: J. Comput. Chem. Jpn 7 Pages: 103-116
  • Peer Reviewed
• [Journal Article] Deletion analysis of the subunit genes of V-type Na+-ATPase from Enterococcus hirae (2006)
  • Author(s): Toshiaki Hosaka, Kazuma Takase, Takeshi Murata, Yoshimi Kakinuma, and Ichiro Yamato
  • Journal Title: Journal of Biochemistry 139 Pages: 1045-1052
  • NAID: 10018844583
  • Peer Reviewed
• [Journal Article] Deletion analysis of the subunit genes of V-type Na^+-ATPase from Enterococcus hirae (2006)
  • Author(s): Toshiaki Hosaka
  • Journal Title: Journal of Biochemistry (Tokyo) 139 Pages: 1045-1052
• [Presentation] Engineering of the rotor ring of the Na+ motive V-ATPase from Enterococcus hirae to examine the stoichiometry (2008)
  • Author(s): 水谷健二
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-03
• [Presentation] Engineering of the rotor ring of the Nat motive V-ATPase from Enterococcus hirae to examine the stoichiometry (2008)
  • Author(s): 水谷健二
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-03
• [Presentation] V型ATPaseのイオン透過機構を考える (2007)
  • Author(s): 村田武士
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] 液胞型イオン輸送ATPアーゼの分子制御とメカニズム (2007)
  • Author(s): 村田武士、山登一郎、柿沼喜己
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会(合同大会)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-15
• [Presentation] ION BINDING AND SELECTIVITY OF THE ROTOR OF THE V-TYPE NA+-ATPASE FROM ENTEROCOCCUS HIRAE (2007)
  • Author(s): Takeshi Murata
  • Organizer: Asian Crystallographic Association 2007
  • Place of Presentation: 台湾(台北)
  • Year and Date: 2007-11-06
• [Presentation] I0N BINDING AND SELECTIVITY OF THE ROTOR OF THE V-TYPE NA^+-ATPASE FROM ENTEROCOCCUS HIRAE (2007)
  • Author(s): 村田 武士
  • Organizer: Asian Crystallographic Association
  • Place of Presentation: 台湾(台北)
  • Year and Date: 2007-11-06
• [Presentation] Ion transport mechanism of V-ATPase (2007)
  • Author(s): Takeshi Murata
  • Organizer: The SOKENDAI International Symposium (37th SERIKEN International Symposium ; 2nd Symposium on the Partnership Project on Frontiers of Membrane Protein Research ; Satellite Symposium of the 84th Annual Meeting of the Physiological Society of JAPAN)
  • Place of Presentation: Okazaki (Japan)
  • Year and Date: 2007-03-15
• [Presentation] V型AT Paseの構造と機能 (2006)
  • Author(s): 村田武士
  • Organizer: 第6回蛋白質科学会
  • Place of Presentation: 京都
  • Year and Date: 2006-04-26
• [Presentation] Structure of Li+ bound rotor ring of the V-type Na+-ATPase from Enterococcus hirae (2006)
  • Author(s): Takeshi Murata
  • Organizer: Gordon Research Conference in Molecular and Cellular Bioenergetics
  • Place of Presentation: New Hampshire( アメリカ)
• [Book] 蛋白質核酸酵素 Vol. 52 No. 4, ,(V-ATPase の新しい機能とその構造からみえてきたメカニズム) (2007)
  • Author(s): 村田武士、山登一郎、柿沼喜己、横山謙
• [Book] V-ATPaseの新しい機能とその構造からみえてきたメカニズム (2007)
  • Author(s): 村田 武士
  • Total Pages: 7
  • Publisher: 蛋白質核酸酵素 共立出版
• [Book] 生物物理、Vol.46, No. 6, (V型Na+-ATPaseの膜内在性ローターリングの構造 : 結晶構造から見えてきた回転モーターのイオン透過機構) (2006)
  • Author(s): 村田武士、山登一郎、柿沼喜己
• [Book] V型Na^+-ATPaseの膜内在性ローターリングの構造 : 結晶構造から見えてきた回転モーターのイオン透過機構 (2006)
  • Author(s): 村田 武士
  • Total Pages: 5
  • Publisher: 日本生物物理学会