Cellular and molecular analyses of fish fin regeneration

• Project/Area Number: 18770197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Tokyo Institute of Technology (2006, 2008); Ochanomizu University (2007)
• Principal Investigator: NAKATANI Yuki Tokyo Institute of Technology, 大学院・生命理工学研究科, 科学研究費研究員 (80397033)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,797,701 (Direct Cost: ¥4,521,309、Indirect Cost: ¥276,392); Fiscal Year 2008: ¥1,197,701 (Direct Cost: ¥921,309、Indirect Cost: ¥276,392); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000); Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 形態形成 / メダカ / 再生 / 発生・分化 / 細胞・組織

Research Abstract

メダカは高い再生能力をもち、特にヒレは切断後わずか10日でほぼ元通りのヒレを再生できる。本研究ではメダカのヒレの再生を細胞レベルおよび分子レベルの両方の側面から理解することを目的とし、研究を進めた。その結果、再生時に起こる個々の細胞現象を明らかにし、また低分子化合物阻害剤を用いた実験から、PI3Kシグナル伝達経路がヒレ再生過程での間充織細胞の移動やヒレの血管新生に関与していることを解明した。

Research Products

• [Journal Article] Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration. Development, Growth and Differentiation (2008)
  • Author(s): Nakatani, Y., Nishidate, M., Fujita, M., Kawakami, A. and Kudo, A.
  • Journal Title: 50 Pages: 71-83
  • Peer Reviewed
• [Journal Article] chongmague reveals an essential role for laminin-mediated boundary formation in chordate convergence and extension. (2008)
  • Author(s): Veeman, M. T., Nakatani, Y., Hendrickson, C., Ericson, V., Lin, C. and Smith, W. C.
  • Journal Title: Development 135 Pages: 33-41
  • Peer Reviewed
• [Journal Article] Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration (2008)
  • Author(s): Nakatani Y., et. al.
  • Journal Title: Development, Growth and Differentiation 50 Pages: 71-83
  • NAID: 10021095489
  • Peer Reviewed
• [Journal Article] chongmague reveals an essential role for laminin-mediated boundary formation in chordate convergence and extension (2008)
  • Author(s): Veeman M. T., et. al.
  • Journal Title: Development 135 Pages: 33-41
  • Peer Reviewed
• [Journal Article] Cellular and molecular process of regeneration, with special emphasis on fish fins. (2007)
  • Author(s): Nakatani, Y., Kawakami, A. and Kudo, A.
  • Journal Title: Development, Growth and Differentiation 49 Pages: 1451-1451
  • NAID: 10018878618
  • Peer Reviewed
• [Journal Article] Identification of novel markers expressed during fin regeneration by microarray analysis in medaka fish. (2007)
  • Author(s): Nishidate, M., Nakatani, Y., Kudo, A. and Kawakami, A.
  • Journal Title: Developmental Dynamics 236 Pages: 2685-2693
  • Peer Reviewed
• [Journal Article] Identification of novel markers expressed during fin regeneration by microarray analysis in medaka fish (2007)
  • Author(s): Nishidate, M., Nakatani, Y., Kudo, A., and Kawakami, A.
  • Journal Title: Developmental Dynamics 236 Pages: 2685-2693
  • Peer Reviewed
• [Journal Article] Cellular and molecular process of regeneration, with special emphasis on fish fins (2007)
  • Author(s): Nakatani, Y., Kawakami, A., Kudo, A.
  • Journal Title: Development, Growth and Differentiation 49 Pages: 145-154
  • NAID: 10018878618
• [Journal Article] Medaka unextended-fin mutants suggests a role of hoxb8a in the cell migration and the osteoblast differentiation during appendage formation. (2006)
  • Author(s): Sakaguchi, S., Nakatani, Y., Yakamatsu, H., Hori, H., Inohaya, K. and Kudo, A.
  • Journal Title: Developmental Biology 293 Pages: 426-438
  • Peer Reviewed
• [Journal Article] Medaka unextended-fin mutants suggest a role of hoxb8a in the cell migration and the osteoblast diggerentiation during appendage formation (2006)
  • Author(s): Sakaguchi, S., Nakatani, Y., Takamatsu, N., Hori, H., Inohaya, K., Kudo, A.
  • Journal Title: Developmental Biology 293 Pages: 426-438
• [Presentation] 魚類におけるヒレ再生メカニズム (2007)
  • Author(s): 中谷友紀、西舘正修、藤田深里、川上厚志、工藤明
  • Organizer: 日本発生生物学会第40回大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-30
• [Presentation] 魚類におけるヒレ再生メカニズム(Regeneration of fish fin) (2007)
  • Author(s): 中谷友紀、西舘正修、藤田深里、川上厚志、工藤明
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2007-05-30
• [Presentation] メダカヒレ再生時の再生芽形成に関与する分子メカニズムの解析 (2006)
  • Author(s): 中谷友紀、西舘正修、藤田深里、川上厚志、工藤明
  • Organizer: 日本動物学会第77回大会
  • Place of Presentation: 島根
  • Year and Date: 2006-09-22
• [Presentation] メダカヒレ再生時に発現誘導される遺伝子群の解析 (2006)
  • Author(s): 西舘正修、中谷友紀、川上厚志、工藤明
  • Organizer: 第12回小型魚類研究会
  • Place of Presentation: 三島
  • Year and Date: 2006-09-16
• [Presentation] メダカ成魚におけるヒレ再生メカニズムの解析 (2006)
  • Author(s): 中谷友紀、西舘正修、川上厚志、工藤明
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 2006-05-30