Three-dimensional exhaustive analysis of cell polarity formation mechanism

• Project/Area Number: 18770207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Keio University
• Principal Investigator: HOTTA Kohji Keio University, 理工学部, 助教 (80407147)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,910,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 形態形成 / 細胞極性 / ホヤ / 三次元 / データベース / イメージング / 発生ステージ / body atlas / 原腸陥入運動

Research Abstract

ホヤ胚における体構築を共焦点レーザー顕微鏡により,3次元的に画像取得し,実際に1細胞レベルの解像度で個体全体を俯瞰できる画像データベースFABAおよびFABA2(http://chordate.bpni.bio.keio.ac.jp/faba2/2.0/top.html)を開発した.さらにこのFABAにおける3次元胚画像をもとに、原腸陥入期における個体全体の細胞の形態変化を定量化し脊索形成に重要な遺伝子の過剰発現胚における形態異常の検出に応用した。その結果、脊索遺伝子の過剰発現胚の形態異常を定量的に検出できた.

Research Products

• [Journal Article] Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos (2009)
  • Author(s): Shigehiro Yamada, Kohji Hotta, Takamasa S. Yamamoto, Naoto Ueno, Nori Satoh and Hiroki Takahashi
  • Journal Title: Developmental Biology (in Press)
  • Peer Reviewed
• [Journal Article] Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos (2009)
  • Author(s): Shigehiro Yamada, Kohji Hotta, Takamasa S. Yamamoto, Naoto Ueno, Nori Satoh and Hiroki Takahashi
  • Journal Title: Developmental Biology (掲載決定)
  • Peer Reviewed
• [Journal Article] Brachyury-downstream gene sets in a primitive chordate, Ciona intestinalis : A link to notochord formation, cell movement and chordate evolution (2008)
  • Author(s): K. Hotta, H. Takahashi, N. Satoh and T. Gojobori
  • Journal Title: Evolution and Development 10(1) Pages: 37-51
  • Peer Reviewed
• [Journal Article] Brachyury-downstream gene sets in a primitive chordate, Ciona intestinalis : A link to notochord formation, cell movement and chordate evolution (2008)
  • Author(s): Kohji Hotta, Hiroki Takahashi, Nori Satoh and Takashi Gojobori
  • Journal Title: Evol. & Dev 10(1) Pages: 37-51
  • Peer Reviewed
• [Journal Article] Brachyury-downstream gene sets in a primitive chordate, Ciona intestinalis:A link tonotochord formation, cell movement and chordate evolution. (2008)
  • Author(s): Kohji Hotta, Hiroki Takahashi, Nori Satoh。d Takashi Gojobori.
  • Journal Title: EvoL & Dev 10(1) Pages: 37-51
  • Peer Reviewed
• [Journal Article] A web-based interactive developmental table for the ascidian Ciona intestinalis, including 3D real-image embryo reconstructions I. From fertilized egg to hatching larva (2007)
  • Author(s): K. Hotta, K. Mitsuhara, H. Takahashi, K. Inaba, K. Oka, T. Gojobori and K. Ikeo
  • Journal Title: Developmental Dynamics 236(7) Pages: 1790-1805
  • Peer Reviewed
• [Journal Article] Brachyury-downstream notochord genes and convergent extension in Ciona intestinalis embryos (2007)
  • Author(s): K. Hotta , S. Yamada, N. Ueno, N. Satoh and H. Takahashi
  • Journal Title: Development, Growth and Differentiation 49(5) Pages: 373-82
  • NAID: 40015432615
  • Peer Reviewed
• [Journal Article] A web-based interactive developmental table for the ascidian CionaintestinEilis, including 3D real-image embryo reconstructions l. Fromfertilized egg to hatching larva (2007)
  • Author(s): Kohii Hotta, Kenta MitsuharEL Hiroki Takahashi, Kazuo Inaba, Kotaro Oka, Takashi Gojobori & Kazuho Ikeo
  • Journal Title: Dev. Dyn. 236(7) Pages: 1790-1805
  • Peer Reviewed
• [Journal Article] Brachjmry-downstreEiin notochord genes and convergent extension in Cionaintestinalis embryos (2007)
  • Author(s): Kohji Hotta , Shigehiro Yamada, Naoto Ueno, Nori Satoh and Hiroki Takahashi
  • Journal Title: DGD 49(5) Pages: 373-82
  • Peer Reviewed
• [Presentation] 3D Virtual Embryoを用いた形態変化の1細胞レベルでの定量解析 (2009)
  • Author(s): 大久保玲子、Delphine Dauga、Francois Robin、Patrick Lemaire、堀田耕司、岡浩太郎
  • Organizer: 動物学会関東支部会
  • Place of Presentation: 埼玉大学
  • Year and Date: 2009-03-20
• [Presentation] Three dimensional real-image embryo reconstructions for the ascidian Ciona intestinalis : II. from hatching larva to juvenile (2008)
  • Author(s): K. Hotta, A. Fukuzawa, K. Mitsuhara, K. Ikeo, T. Gojobori, K. Inaba and K. Oka
  • Organizer: Joint Meeting of the SFBD and the JSDB, "Fron tiers in Developmental Biology
  • Place of Presentation: フランス, Giens peninsula, Hyerres
  • Year and Date: 2008-09-15
• [Presentation] Three dimensional real-image embryo reconstructions for the ascidian Ciona intestinalis : II. from hatching larva to juvenile (2008)
  • Author(s): K. Hotta, A. Fukuzawa, K. Mitsuhara, K. Ikeo, T. Gojobori, K. Inaba and K. Oka
  • Organizer: Joint Meeting of the SFBD and the JSDB
  • Place of Presentation: フランス,Giens peninsula, Hyeres
  • Year and Date: 2008-09-14
• [Presentation] Quantitative analysis of morphogenetic change during gastrulation movement in ascidian Ciona intestinalis by constructing 3-D virtual embryo 3次元バーチャル胚構築によるホヤ胚原腸陥入時における形態変化の1細胞レベルでの定量解析 (2008)
  • Author(s): 大久保玲子、Delphine Dauga、Francois Robin、Patrick Lemaire、堀田耕司、岡浩太郎
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学工学部IB電子情報館
  • Year and Date: 2008-09-04
• [Presentation] 3次元バーチャル胚構築によるホヤ胚原腸陥入時における形態変化の1細胞レベルでの定量解析 (2008)
  • Author(s): 大久保玲子、DelphineDauga、FrancoisRobin、PatrickLemaire、堀田耕司、岡浩太郎
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学工学部IB電子情報館
  • Year and Date: 2008-09-04
• [Presentation] Novel Peripheral Neuronal Structure Revealed by Confocal and Transmission Electron Microscopies in Ascidian Larvae共焦点顕微鏡および透過型電子顕微鏡によって明らかにされたホヤ幼生新規末梢神経構造 (2008)
  • Author(s): 寺久保洋、中島陽子、堀田耕司、岡浩太郎
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島文化芸術会館
  • Year and Date: 2008-05-28
• [Presentation] カタユウレイボヤ初期発生の新ステージングと四次元胚発生画像データベースFABA (2007)
  • Author(s): 堀田耕司、岡浩太郎、五條堀孝、光原健太、高橋弘樹、稲葉一男、池尾一穂
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-20
• [Presentation] Developmental table for the ascidian Ciona intestinalis using 3D real image embryo : I. From fertilized egg to hatching larva (2007)
  • Author(s): Kohji Hotta, Kenta Mitsuhara, Hiroki Takahashi, Kazuo Inaba, Kotaro Oka, Takashi Gojobori & Kazuho Ikeo
  • Organizer: 4th International Tunicate Meeting
  • Place of Presentation: フ ランス、Villefranche-sur-Mer, Observatoire Oceanologique, Saint-Jean Cap Ferrat, Residence Delcloy
  • Year and Date: 2007-06-25
• [Presentation] Brachyury-downstream notochord genes and convergent extension in Ciona intestinalis embryos (2007)
  • Author(s): Kohji Hotta, Shigehiro Yamada, Naoto Ueno, Nori Satoh and Hiroki Takahashi
  • Organizer: 第40回日本発生生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-29
• [Presentation] カタユウレイボヤ初期発生の新ステージングと三次元胚発生画像データベース (2007)
  • Author(s): 堀田耕司・光原健太・高橋弘樹・稲葉一男・岡浩太郎・五條堀孝・池尾一穂
  • Organizer: 第2回ホヤ研究集会
  • Place of Presentation: 下田東急ホテル
• [Presentation] Developmental table R)r the ascidian Ciona intestinal,£y using 3D real image embryo:I.From fertilized egg to hatching larva (2007)
  • Author(s): Kohji Hotta, Kenta Mitsuhara, Hiroki Takahashi, Kazuo Inaba, Kotaro Oka, Takashi Gojobori &Ka2MhoIkeo
  • Organizer: 4th International Tunicate Meeting
  • Place of Presentation: フランス、Villefianche-sur-Mer
• [Presentation] カタユウレイボヤ初期発生の新ステージンクと三次元胚発生画像データベース (2007)
  • Author(s): 堀田 耕司・光原 健太・高橋 弘樹・稲葉 一男・岡浩 太郎・五條 堀孝・池尾 一穂
  • Organizer: 第2回ホヤ研究集会
  • Place of Presentation: 下田東急ホテル
• [Presentation] Brachyu-downstream notochord genes and convergent extension in Ciona intestinalisembryos (2007)
  • Author(s): Kohji Hotta. Shigehiro Yamada, Naoto Ueno, Nori Satoh and Hiroki Takahashi
  • Organizer: 第40回日本発生生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] カタユウレイボヤ初期発生の新ステージンクと四次元胚発生画像データベースFABA (2007)
  • Author(s): 堀田 耕司、岡浩 太郎、五條 堀孝、光原 健太、高橋 弘樹、稲葉 一男、池尾 一穂
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
• [Remarks] CINOBI データベース
  • URL: http://hottapc.lab.nig.ac.jp/notochord/cibra/cibradownhome.html
• [Remarks] FABA1 データベース
  • URL: http://chordate.bpni.bio.keio.ac.jp/faba1/top.html
• [Remarks] FABA2 データベース
  • URL: http://chordate.bpni.bio.keio.ac.jp/faba2/2.0/top.html
• [Remarks] FABA2データベース
  • URL: http://chordate.bpni.bio.keio.ac.jp/faba2/2.0Ntop.html
• [Remarks] FABAデータベース
  • URL: http://chordate.bpni.bio.keio.ac.jp/faba/1.1/top.html
• [Remarks] CINOBIデータベース
  • URL: http://hottapc.lab.nig.ac.jp/notochord/cibra/