Development of Ds insertion barley lines via Agrobacterium-mediated transformation

• Project/Area Number: 18780005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Okayama University
• Principal Investigator: SAISHO Daisuke Okayama University, 資源生物科学研究所, 助教 (90325126)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,840,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: オオムギ・形質転換・トランスポゾン / オオムギ / 形質転換 / トランスポゾン

Research Abstract

生物の設計図といわれるゲノムの全塩基配列が次々と明らかになるにつれて,生物が持つ機能や応用に向けた研究は大きく様変わりし,その情報を利用して研究を進める上で必須の研究手法やリソースの整備が求められている.本研究では,世界的に主要な農作物であるオオムギを材料に,ゲノム科学的な研究を進める上で必須と考えられる形質転換法の確立と,それを利用した突然変異体パネルの構築に向けた基礎的な研究を行った.

Research Products

• [Journal Article] Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos (2008)
  • Author(s): Rikiishi, K., D. Saisho K. Takeda
  • Journal Title: Breed. Sci. 58 Pages: 149-155
  • NAID: 110006781641
  • Peer Reviewed
• [Journal Article] TriMEDB : A database to integrate transcribed markers and facilitate genetic studies of the tribe Triticeae (2008)
  • Author(s): Mochida, Saisho, Yoshida, Sakurai and Shinozaki
  • Journal Title: BMC Plant Biology 8 Pages: 72-72
  • Peer Reviewed
• [Journal Article] Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos (2008)
  • Author(s): Rikiishi. K., D. Saisho. K. Takeda
  • Journal Title: Breed. Sci. 58 Pages: 149-155
  • NAID: 110006781641
  • Peer Reviewed
• [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor generegulating a lipid biosynthesis pathway (2008)
  • Author(s): Taketa, Amano, Tsujino, Sato, Saisho, Kakeda, Nomura, Suzuki, Matsumoto, Sato. Kanamori, Kawasaki and Takeda
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 4062-4067
  • Peer Reviewed
• [Journal Article] Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World. (2007)
  • Author(s): D. Saisho and M. D. Purugganan
  • Journal Title: GENETICS 177 Pages: 1765-1776
  • Peer Reviewed
• [Journal Article] 草型変異から見たイネとオオムギの栽培化 (2007)
  • Author(s): 浅野賢治・最相大輔・芦苅基行・松岡信
  • Journal Title: 蛋白質 核酸 酵素 Vol.52 Pages: 1931-1936
• [Journal Article] Construction and Characterization of a Bacterial Artificial Chromosome(BAC)Library from Japanese Malting Barley 'Haruna Nijo'. (2007)
  • Author(s): Saisho, D., E. Myoraku, S. Kawasaki, K. Sato and K. Takeda
  • Journal Title: Breed. Sci. 54 Pages: 409-416
  • Peer Reviewed
• [Journal Article] Molecular phylogeography of domesticated barley traces expansion of agricultue in the Old World. (2007)
  • Author(s): D. Saisho and Purugganan, M. D.
  • Journal Title: GENETICS 177 Pages: 1765-1776
  • Peer Reviewed
• [Journal Article] 草型変異から見たイネとオオムギの栽培化. (2007)
  • Author(s): 浅野賢治・最相大輔・芦苅基行・松岡信
  • Journal Title: 蛋白質 核酸 酵素 52 Pages: 1931-1936
• [Journal Article] Construction and characterization of a bacterial artificial chromosome (BAC) library for Japanese malting barley 'Haruna Nijo'. (2007)
  • Author(s): Saisho, D., E.Myoraku, S.Kawasaki, K.Sato, K.Takeda
  • Journal Title: Breed.Sci. 57 Pages: 29-38