Analysis of cell wall degrading enzymes of Bursaphelenchus xylophilus using RNA interference

• Project/Area Number: 18780032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: KIKUCHI Taisei Forestry and Forest Products Research Institute, 森林微生物研究領域, 研究員 (20353659)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 遺伝子 / RNA干渉 / マツノザイセンチュウ / 細胞壁 / セルラーゼ / 植物寄生 / 糸状菌

Research Abstract

マツノザイセンチュウからセルラーゼ、ベータ1,3グルカナーゼおよびペクチン分解酵素など、数種類の細胞壁分解酵素遺伝子を単離した。組み替えタンパクを大腸菌または酵母を用いて作成し精製した後、生化学的な性質を明らかにした。また、マツノザイセンチュウへのRNA干渉法を検討し、有効な誘導法についての知見を得た。

Research Products

• [Journal Article] An inexpensive high-throughput method to extract high yields of good quality DNA from fungi (2009)
  • Author(s): Kikuchi, T., Karim, N., Masuya, H., Ota, Y., and Kubono, T.
  • Journal Title: Molecular ecology resources 9 Pages: 41-45
• [Journal Article] Purification and characterization of recombinant endoglucanases from the pine wood nematode Bursaphelenchus xylophilus (2008)
  • Author(s): Shibuya, H., and Kikuchi, T.
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 1325-1332
  • NAID: 10027527989
• [Journal Article] Bursaphelenchus xylophilus : opportunities in comparative genomics and molecular host-parasite interactions (2008)
  • Author(s): Jones, J. T., Moens, M., Mota, M., Li, H., and Kikuchi, T.
  • Journal Title: Molecular Plant Pathology 9
• [Journal Article] Purification and characterization of recombinant endoglucanases from the pine wood nematode Bursaphelenchus xylophilus (2008)
  • Author(s): Shibuya, H, and Kikuchi, T.
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 1325-1332
  • NAID: 10027527989
  • Peer Reviewed
• [Journal Article] Estimation of virulence of Bursaphelenchus xylophilus (Nematoda : Aphelenchoididae) based on its reproductive ability (2007)
  • Author(s): Aikawa, T., and Kikuchi, T.
  • Journal Title: Nematology 9 Pages: 371-377
• [Journal Article] Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B. mucronatus (2007)
  • Author(s): Kikuchi, T., Aikawa, T., Kosaka, H., Pritchard, L., Ogura, N., and Jones, J. T
  • Journal Title: Mol Biochem Parasitol 155 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B. mucronatus. (2007)
  • Author(s): Kikuchi, T., Aikawa, T., Kosaka, H., Pritchard, L., Ogura, N. and Jones, J. T.
  • Journal Title: Mol Biochem Parasitol 155 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Population structure of Bursaphelenchus xylophilus within single Pinus thunbergii trees inoculated with two nematode isolates (2006)
  • Author(s): Aikawa, T., Kikuchi, T., and Kosaka, H.
  • Journal Title: Forest Pathology 36 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Cloning and characterization of pectate lyases expressed in the esophageal gland of the pine wood nematode Bursaphelenchus xylophilus (2006)
  • Author(s): Kikuchi, T., Shibuya, H., Aikawa, T., and Jones, J. T.
  • Journal Title: Molecular Plant-Microbe Interactions 19 Pages: 280-287
  • Peer Reviewed
• [Journal Article] Cloning and characterization of pectate lyases expressed in the esophageal gland of the pine wood nematode Bursaphelenchus xylophilus. (2006)
  • Author(s): Kikuchi, T., Shibuya, H., Aikawa, T., Jones, J.T.
  • Journal Title: Molecular Plant-microbe Interactions 19 Pages: 280-287
• [Presentation] EST analysis of the fungivorous nematode Aphelenchus avenae (2008)
  • Author(s): Karim, N., and Kikuchi, T
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: Brisbane, Australia
• [Presentation] Analysis of expressed sequence tags (ESTs) of the fungivorous nematode Aphelenchus avenae (2008)
  • Author(s): Karim, N., Okada, H., and Kikuchi, T
  • Organizer: 16th Annual Meeting of the Japanese Nematological Society
  • Place of Presentation: Tsukuba
• [Presentation] EST Analysis of the Pine Wood Nematode Bursaphelenchus xylophilus and Functional Analysis of Parasitism Genes in this Species (2008)
  • Author(s): Kikuchi, T
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: Brisbane, Australia
• [Presentation] EST Analysis of Bursaphelenchus xylophilus and Functional Analysis of Parasitism Genes in this Species (2008)
  • Author(s): Taisei Kikuchi
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: ブリスベン
• [Presentation] EST analysis of the fungivorous nematode Aphelenchus avenae (2008)
  • Author(s): Nurul Karim and Taisei Kikuchi
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: ブリスベン
• [Presentation] Analysis of expressed sequence tags (ESTs) of the fungivorous nematode Aphelenchus avenae (2008)
  • Author(s): Karim, N., Okada, H., and Kikuchi, T.
  • Organizer: 16th Annual Meeting of the Japanese Nematological Society
  • Place of Presentation: つくば
• [Presentation] Parasitism genes of the pinewood nematode (2007)
  • Author(s): Kikuchi, T
  • Organizer: International symposium on Pine Wilt Disease in Asia
  • Place of Presentation: Kyoto, Japan
• [Presentation] Parasitism genes of the pinewood nematode. (2007)
  • Author(s): Taisei Kikuchi
  • Organizer: International symposium on Pine Wilt Disease in Asia
  • Place of Presentation: 京都
• [Presentation] マツノザイセンチュウのペクチン分解酵素遺伝子 (2006)
  • Author(s): Kikuchi, T., Shibuya, H., Aikawa, T., and Jones, J. T.
  • Organizer: 第117回日本森林学会大会
• [Book] In Pine Wilt Disease, B. G. Zhao, K. Futai, J. Sutherland, and Y. Takeuchi, eds(Springer) (2008)
  • Author(s): Kikuchi, T
  • Publisher: Parasitism Genes of the Pine Wood Nematode
• [Book] Pine Wilt Disease (2008)
  • Author(s): Taisei Kikuchi
  • Publisher: Springer Japan
• [Remarks] ForestGen
  • URL: http://forestgen.ffpri.affrc.go.jp/ja/index.html