Analysis of cell wall degrading enzymes of Bursaphelenchus xylophilus using RNA interference
Project/Area Number |
18780032
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Plant pathology
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Research Institution | Forestry and Forest Products Research Institute |
Principal Investigator |
KIKUCHI Taisei Forestry and Forest Products Research Institute, 森林微生物研究領域, 研究員 (20353659)
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Project Period (FY) |
2006 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
Keywords | 遺伝子 / RNA干渉 / マツノザイセンチュウ / 細胞壁 / セルラーゼ / 植物寄生 / 糸状菌 |
Research Abstract |
マツノザイセンチュウからセルラーゼ、ベータ1,3グルカナーゼおよびペクチン分解酵素など、数種類の細胞壁分解酵素遺伝子を単離した。組み替えタンパクを大腸菌または酵母を用いて作成し精製した後、生化学的な性質を明らかにした。また、マツノザイセンチュウへのRNA干渉法を検討し、有効な誘導法についての知見を得た。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B. mucronatus2007
Author(s)
Kikuchi, T., Aikawa, T., Kosaka, H., Pritchard, L., Ogura, N., and Jones, J. T
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Journal Title
Mol Biochem Parasitol 155
Pages: 9-17
Related Report
Peer Reviewed
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[Journal Article] Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B. mucronatus.2007
Author(s)
Kikuchi, T., Aikawa, T., Kosaka, H., Pritchard, L., Ogura, N. and Jones, J. T.
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Journal Title
Mol Biochem Parasitol 155
Pages: 9-17
Related Report
Peer Reviewed
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