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Establishment and characterization of immortalized cell lines derived from bovine brain tissues

Research Project

Project/Area Number 18780081
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

TAKENOUCHI Takato  National Institute of Agrobiological Sciences, 遺伝子組換え家畜研究センター, 主任研究員 (20292518)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,180,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Keywordsウシ脳由来細胞株 / 内皮細胞様細胞株 / 神経様細胞株 / プリオン蛋白 / BSE / ウシプリオン蛋白 / 新規ウシ細胞株 / 神経前駆細胞 / プリオン蛋白質 / ウシ細胞株 / 内皮細胞 / 平滑筋細胞
Research Abstract

本研究ではウシ胎子脳から機能性細胞株を樹立し、その特性解析を行うとともに牛海綿状脳症(BSE)研究への利用を検討した。その結果、内皮細胞様及び神経細胞様の性質を持つ新しいウシ脳由来細胞株を樹立した。これらの細胞には正常ウシプリオン蛋白の発現が確認され、BSE 研究への利用も期待された。そこでBSE プリオン感染実験を行ったが、現在までのところ持続感染細胞の樹立には至っておらず引き続き検討が必要である。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (21 results)

All 2009 2008 2007

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (8 results)

  • [Journal Article] The activation of P2X7 receptor impairs lysosomal functions and stimulates the release of autophagolysosomes in microglial cells.2009

    • Author(s)
      Takenouchi T., Nakai M., Iwamaru Y.,Sugama S., Tsukimoto M., Fujita M., Wei J.,Sekigawa A., Sato M., Kojima S., Kitani H.,and Hashimoto M.
    • Journal Title

      J. Immunol 182

      Pages: 2051-2062

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of an SV40 large T antigen-immortalized bovine brain cell line and its neuronal differentiation by dibutyryl cyclic AMP.2009

    • Author(s)
      Takenouchi T., Iwamaru Y., Sato M.,Yokoyama T., Kitani H
    • Journal Title

      Cell Biol. Int 33

      Pages: 187-191

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The activation of P2X7 receptor impairs lysosomal functions and stimulates the release of autophagolysosomes in microglial cells.2009

    • Author(s)
      Takenouchi T., Nakai M., Iwamaru Y., Sugama S., Tsukimoto M., Fujita M., Wei J., Sekigawa A., Sato M., Kojima S., Kitani H., Hashimoto M.
    • Journal Title

      J. Immunol. 182

      Pages: 2051-2062

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of an SV40 large T antigen-immortalized bovine brain cell line and its neuronal differentiation by dibutyryl cyclic AMP.2009

    • Author(s)
      Takenouchi T, Iwamaru Y, Sato M, Yokoyama T, Kitani H
    • Journal Title

      Cell Biol. Int. 33

      Pages: 187-191

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミクログリア細胞株を用いたプリオン持続感染モデル系の作出とその特性解析2008

    • Author(s)
      竹之内敬人、岩丸祥史、横山隆、木谷裕,
    • Journal Title

      生化学 80巻7号

    • NAID

      10021924827

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lysophospholipids and ATP mutually suppress maturation and release of IL-1υ in mouse microglial cells using a Rho-dependent pathway.2008

    • Author(s)
      Takenouchi T., Iwamaru Y., Sugama S., Sato M., Hashimoto M., and Kitani H.
    • Journal Title

      J. Immunol. 180

      Pages: 7827-7839

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lysophospholipids and ATP mutually suppress maturation and release of IL-1β in mouse microglial cells using a Rho-dependent pathway.2008

    • Author(s)
      Takenouchi T., Iwamaru Y., Sugama S., Sato M., Hashimoto M., Kitani H.
    • Journal Title

      J. Immunol. 180

      Pages: 7827-7839

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lysophosphatidylcholine potentiates Ca^<2+> influx, pore formation and p44/42 MAP kinase phosphorylation mediated by P2X7 receptor activation in mouse microglial cells.2007

    • Author(s)
      Takenouchi T., Sato M., Kitani H.
    • Journal Title

      J. Neurochem. 102

      Pages: 1518-1532

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line.2007

    • Author(s)
      Takenouchi T., Iwamaru Y., Imamura M., Kato N., Sugama S., Fujita M., Hashimoto M., Sato M., Okada H., Yokoyama T., Mohri S, Kitani H
    • Journal Title

      FEBS Lett. 581

      Pages: 3019-3026

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment and characterization of SV40 large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation.2007

    • Author(s)
      Takenouchi T., Iwamaru Y., Sato M., Yokoyama T., Shinagawa M., Kitani H.
    • Journal Title

      Cell Biol. Int 31

      Pages: 57-64

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in amouse microglial cell line.2007

    • Author(s)
      Takenouchi T, Iwamaru Y, Imamura M, Kato N, Sugama S, Fujita M, Hashimoto M, Sato M, Okada H, Yokoyama T, Mohri S, Kitani H.
    • Journal Title

      FEBS Lett. 581

      Pages: 3019-3026

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lysophosphatidylcholine potentiates Ca^2+ influx, pore formation and p44/42 MAP kinase phosphorylation mediated by P2X7 receptor activation in mouse microglial cells.2007

    • Author(s)
      Takenouchi T, Sato M, Kitani H.
    • Journal Title

      J. Neurochem. 102

      Pages: 1518-1532

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment and characterization of SV40large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation2007

    • Author(s)
      Takenouchi T, Iwamaru Y, Sato M, Yokoyama T, Shinagawa M, Kitani H
    • Journal Title

      Cell Biology International 31(1)

      Pages: 57-64

    • Related Report
      2006 Annual Research Report
  • [Presentation] ミクログリアでのATPによる成熟型インターロイキシ-1β産生に対するリゾリン脂質の効果2009

    • Author(s)
      竹之内敬人,木谷裕
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] ミクロダリアでのATPによる成熟型インターロイキシー1β産生に対するリゾリン脂質の効果2009

    • Author(s)
      竹之内敬人, 木谷裕
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] リゾリン脂質とATPはミクログリアにおける成熟型インターロイキシ-1βの産生を互いに抑制する2008

    • Author(s)
      竹之内敬人, 岩丸祥史, 洲鎌秀永, 佐藤充, 橋本款, 木谷裕
    • Organizer
      BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-08
    • Related Report
      2008 Final Research Report
  • [Presentation] リゾリン脂質とATPはミクログリアにおける成熟型インターロイキシー1βの産生を互いに抑制する2008

    • Author(s)
      竹之内敬人, 岩丸祥史, 洲鎌秀永, 佐藤充, 橋本款, 木谷裕
    • Organizer
      BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] リゾホスファチジルコリンによるミクログリアP2X7受容体の機能調節2008

    • Author(s)
      竹之内敬人、佐藤充、木谷裕
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-26
    • Related Report
      2008 Final Research Report
  • [Presentation] リゾボスファチジルコリンによるミクログリアP2X7受容体の機能調節2008

    • Author(s)
      竹之内敬人、 佐藤充、 木谷裕
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Potentiation of nucleotides-induced P2X7 receptor activation by lysophosphatidylcholine in mouse microglial cells.2007

    • Author(s)
      Takenouchi T, Sugama S, Sato M, Kitani H
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      サンディエゴ(米国)
    • Year and Date
      2007-11-07
    • Related Report
      2008 Final Research Report
  • [Presentation] Potentiation of nucleotides-induced P2X7 receptor activation by lysophosphatidylcholine in mouse microglial cells2007

    • Author(s)
      Takenouchi T, Sugama S, Sato M, Kitani H
    • Organizer
      Society for Neuroscience 2007
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2007-11-07
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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