Establishment and characterization of immortalized cell lines derived from bovine brain tissues

• Project/Area Number: 18780081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: TAKENOUCHI Takato National Institute of Agrobiological Sciences, 遺伝子組換え家畜研究センター, 主任研究員 (20292518)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,180,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: ウシ脳由来細胞株 / 内皮細胞様細胞株 / 神経様細胞株 / プリオン蛋白 / BSE / ウシプリオン蛋白 / 新規ウシ細胞株 / 神経前駆細胞 / プリオン蛋白質 / ウシ細胞株 / 内皮細胞 / 平滑筋細胞

Research Abstract

本研究ではウシ胎子脳から機能性細胞株を樹立し、その特性解析を行うとともに牛海綿状脳症(BSE)研究への利用を検討した。その結果、内皮細胞様及び神経細胞様の性質を持つ新しいウシ脳由来細胞株を樹立した。これらの細胞には正常ウシプリオン蛋白の発現が確認され、BSE 研究への利用も期待された。そこでBSE プリオン感染実験を行ったが、現在までのところ持続感染細胞の樹立には至っておらず引き続き検討が必要である。

Research Products

• [Journal Article] The activation of P2X7 receptor impairs lysosomal functions and stimulates the release of autophagolysosomes in microglial cells. (2009)
  • Author(s): Takenouchi T., Nakai M., Iwamaru Y.,Sugama S., Tsukimoto M., Fujita M., Wei J.,Sekigawa A., Sato M., Kojima S., Kitani H.,and Hashimoto M.
  • Journal Title: J. Immunol 182 Pages: 2051-2062
  • Peer Reviewed
• [Journal Article] Establishment of an SV40 large T antigen-immortalized bovine brain cell line and its neuronal differentiation by dibutyryl cyclic AMP. (2009)
  • Author(s): Takenouchi T., Iwamaru Y., Sato M.,Yokoyama T., Kitani H
  • Journal Title: Cell Biol. Int 33 Pages: 187-191
  • Peer Reviewed
• [Journal Article] The activation of P2X7 receptor impairs lysosomal functions and stimulates the release of autophagolysosomes in microglial cells. (2009)
  • Author(s): Takenouchi T., Nakai M., Iwamaru Y., Sugama S., Tsukimoto M., Fujita M., Wei J., Sekigawa A., Sato M., Kojima S., Kitani H., Hashimoto M.
  • Journal Title: J. Immunol. 182 Pages: 2051-2062
  • Peer Reviewed
• [Journal Article] Establishment of an SV40 large T antigen-immortalized bovine brain cell line and its neuronal differentiation by dibutyryl cyclic AMP. (2009)
  • Author(s): Takenouchi T, Iwamaru Y, Sato M, Yokoyama T, Kitani H
  • Journal Title: Cell Biol. Int. 33 Pages: 187-191
  • Peer Reviewed
• [Journal Article] ミクログリア細胞株を用いたプリオン持続感染モデル系の作出とその特性解析 (2008)
  • Author(s): 竹之内敬人、岩丸祥史、横山隆、木谷裕,
  • Journal Title: 生化学 80巻7号
  • NAID: 10021924827
  • Peer Reviewed
• [Journal Article] Lysophospholipids and ATP mutually suppress maturation and release of IL-1υ in mouse microglial cells using a Rho-dependent pathway. (2008)
  • Author(s): Takenouchi T., Iwamaru Y., Sugama S., Sato M., Hashimoto M., and Kitani H.
  • Journal Title: J. Immunol. 180 Pages: 7827-7839
  • Peer Reviewed
• [Journal Article] Lysophospholipids and ATP mutually suppress maturation and release of IL-1β in mouse microglial cells using a Rho-dependent pathway. (2008)
  • Author(s): Takenouchi T., Iwamaru Y., Sugama S., Sato M., Hashimoto M., Kitani H.
  • Journal Title: J. Immunol. 180 Pages: 7827-7839
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine potentiates Ca^<2+> influx, pore formation and p44/42 MAP kinase phosphorylation mediated by P2X7 receptor activation in mouse microglial cells. (2007)
  • Author(s): Takenouchi T., Sato M., Kitani H.
  • Journal Title: J. Neurochem. 102 Pages: 1518-1532
  • Peer Reviewed
• [Journal Article] Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line. (2007)
  • Author(s): Takenouchi T., Iwamaru Y., Imamura M., Kato N., Sugama S., Fujita M., Hashimoto M., Sato M., Okada H., Yokoyama T., Mohri S, Kitani H
  • Journal Title: FEBS Lett. 581 Pages: 3019-3026
  • Peer Reviewed
• [Journal Article] Establishment and characterization of SV40 large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation. (2007)
  • Author(s): Takenouchi T., Iwamaru Y., Sato M., Yokoyama T., Shinagawa M., Kitani H.
  • Journal Title: Cell Biol. Int 31 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in amouse microglial cell line. (2007)
  • Author(s): Takenouchi T, Iwamaru Y, Imamura M, Kato N, Sugama S, Fujita M, Hashimoto M, Sato M, Okada H, Yokoyama T, Mohri S, Kitani H.
  • Journal Title: FEBS Lett. 581 Pages: 3019-3026
  • Peer Reviewed
• [Journal Article] Lysophosphatidylcholine potentiates Ca^2+ influx, pore formation and p44/42 MAP kinase phosphorylation mediated by P2X7 receptor activation in mouse microglial cells. (2007)
  • Author(s): Takenouchi T, Sato M, Kitani H.
  • Journal Title: J. Neurochem. 102 Pages: 1518-1532
  • Peer Reviewed
• [Journal Article] Establishment and characterization of SV40large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation (2007)
  • Author(s): Takenouchi T, Iwamaru Y, Sato M, Yokoyama T, Shinagawa M, Kitani H
  • Journal Title: Cell Biology International 31(1) Pages: 57-64
• [Presentation] ミクログリアでのATPによる成熟型インターロイキシ-1β産生に対するリゾリン脂質の効果 (2009)
  • Author(s): 竹之内敬人,木谷裕
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] ミクロダリアでのATPによる成熟型インターロイキシー1β産生に対するリゾリン脂質の効果 (2009)
  • Author(s): 竹之内敬人, 木谷裕
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] リゾリン脂質とATPはミクログリアにおける成熟型インターロイキシ-1βの産生を互いに抑制する (2008)
  • Author(s): 竹之内敬人, 岩丸祥史, 洲鎌秀永, 佐藤充, 橋本款, 木谷裕
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-08
• [Presentation] リゾリン脂質とATPはミクログリアにおける成熟型インターロイキシー1βの産生を互いに抑制する (2008)
  • Author(s): 竹之内敬人, 岩丸祥史, 洲鎌秀永, 佐藤充, 橋本款, 木谷裕
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-08
• [Presentation] リゾホスファチジルコリンによるミクログリアP2X7受容体の機能調節 (2008)
  • Author(s): 竹之内敬人、佐藤充、木谷裕
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-26
• [Presentation] リゾボスファチジルコリンによるミクログリアP2X7受容体の機能調節 (2008)
  • Author(s): 竹之内敬人、 佐藤充、 木谷裕
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-26
• [Presentation] Potentiation of nucleotides-induced P2X7 receptor activation by lysophosphatidylcholine in mouse microglial cells. (2007)
  • Author(s): Takenouchi T, Sugama S, Sato M, Kitani H
  • Organizer: Society for Neuroscience
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2007-11-07
• [Presentation] Potentiation of nucleotides-induced P2X7 receptor activation by lysophosphatidylcholine in mouse microglial cells (2007)
  • Author(s): Takenouchi T, Sugama S, Sato M, Kitani H
  • Organizer: Society for Neuroscience 2007
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-07