Research on male sterility-related genes of sugi (Cryptomeria japonica D. Don) utilizing EST data

• Project/Area Number: 18780123
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 林学・森林工学
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: FUTAMURA Norihiro Forestry and Forest Products Research Institute, 生物工学研究領域, 主任研究員 (80343804)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: スギ / 雄性不稔 / 遺伝子発現 / 雄花 / 花粉形成 / 遺伝子

Research Abstract

スギの雄性不稔形質に関連する遺伝子を探索するため、既知の雄性不稔遺伝子と相同なスギ遺伝子を解析するとともに、雄性不稔個体と正常個体の雄花で発現する遺伝子の網羅的解析を行った。スギ遺伝子の網羅的解析には、既知のスギEST情報を利用して作製したDNAマイクロアレイを用いた。原因遺伝子の特定には至らなかったが、雄性不稔に関連する遺伝子を絞り込むことができた。

Research Products

• [Journal Article] Characterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili (2008)
  • Author(s): Norihiro Futamura、Yasushi Totoki、Atsushi Toyoda、Tomohiro Igasaki、Tokihiko Nanjo、Motoaki Seki、Yoshiyuki Sakaki、Adriano Mari、Kazuo Shinozaki、Kenji Shinohara
  • Journal Title: BMC Genomics 9 Pages: 383-383
  • Peer Reviewed
• [Journal Article] Characterization of expressed sequence tags from a full-length enriched cDNA library of Czyptomeria japonica male strobili (2008)
  • Author(s): Norihiro Futamura
  • Journal Title: BMC Genomics 9 Pages: 383-383
  • Peer Reviewed
• [Journal Article] Characterization of genes for a pollen allergen, Cry j 2, of Cryptomeria japonica (2007)
  • Author(s): Norihiro Futamura、Yasunobu Kusunoki、Yuzuru Mukai、Kenji Shinohara
  • Journal Title: International Archives of Allergy and Immunology 143 Pages: 59-68
  • Peer Reviewed
• [Journal Article] Characterization of genes for a pollen allergen, Cry j 2,of Czyptomeria japonica. (2007)
  • Author(s): Norihiro Futamura
  • Journal Title: International Archives of Allergy and Immunology 143 Pages: 59-68
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags from Cryptomeria japonica pollen reveals novel pollen-specific transcripts (2006)
  • Author(s): Norihiro Futamura、Tokuko Ujino-Ihara、Mitsuru Nishiguchi、Hiroyuki Kanamori、Kensuke Yoshimura、Masahiro Sakaguchi、Kenji Shinohara
  • Journal Title: Tree Physiology 26 Pages: 1517-1528
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags from Cryptomeria japonica pollen reveals novel pollen-specific transcripts. (2006)
  • Author(s): Norihiro Futamura
  • Journal Title: Tree Physiology 26・12 Pages: 1517-1528
• [Presentation] 有用形質を支配する遺伝子座の単離に向けたスギ基盤連鎖地図の高度化 (2009)
  • Author(s): 森口喜成
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-03-26
• [Presentation] スギ花粉の発達過程における発現遺伝子の網羅的解析 (2009)
  • Author(s): 二村典宏
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-03-26
• [Presentation] 樹木のバイオリソース (2009)
  • Author(s): 篠原健司
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-24
• [Presentation] マイクロアレイを用いたスギ雄性不稔に関連する遺伝子群の解析 (2009)
  • Author(s): 二村典宏
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] スギ雄性不稔家系の花粉発達過程に発現する遺伝子の網羅的解析 (2008)
  • Author(s): 二村典宏
  • Organizer: 日本森林学会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2008-03-28
• [Presentation] スギ雄花形成の機構解明 (2008)
  • Author(s): 篠原健司
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学
  • Year and Date: 2008-03-27
• [Presentation] スギ雄花で発現する遺伝子群の解析 (2007)
  • Author(s): 二村典宏
  • Organizer: 日本森林学会
  • Place of Presentation: 九州大学
  • Year and Date: 2007-04-02
• [Presentation] スギ雄花完全長cDNAの大規模収集 (2007)
  • Author(s): 二村典宏
  • Organizer: 日本植物生理学会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2007-03-29
• [Presentation] Characterization of expressed sequence tags from the pollen of Cryptomeria japonica (2006)
  • Author(s): Norihiro Futamura
  • Organizer: 8th INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY
  • Place of Presentation: アデレード
  • Year and Date: 2006-08-22
• [Remarks]
  • URL: http://forestgen.ffpri.affrc.go.jp
• [Remarks]
  • URL: http://forestgen.ffpri.affrc.go.jp