Molecular cloning of the gene related to the disease resistance of bacterial coldwater disease in Ayu (Plecoglossus altivelis)

• Project/Area Number: 18780145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: SAKAMOTO Takashi Tokyo University of Marine Science and Technology, 海洋科学部, 准教授 (40313390)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 遺伝 / 育種 / マーカー選抜育種 / 遺伝子地図 / 耐病性 / ゲノム育種 / 魚類 / 連鎖解析 / マイクロサテライト / アユ / 冷水病 / ゲノム

Research Abstract

アユ冷水病耐性形質を識別可能なDNAマーカーを用いて、実験魚各個体の耐病性形質保有の有無を推定・識別した。人為的な冷水病菌感染後、1時間および3時間の脾臓において、耐病性形質を保持する個体群に特有の発現パターンを示す遺伝子断片を単離した。耐病性形質を保持する個体群に特有の発現パターンを示す遺伝子断片は、アユにおける冷水病に対する耐病性メカニズムに関与する遺伝子であると考えられた。

Research Products

• [Journal Article] Marker-assisted breeding of a lymphocystis disease-resistant Japaneseflounder(Paralichthys olivaceus) (2007)
  • Author(s): K. Fuji, et. al.
  • Journal Title: Aquaculture 272 Pages: 291-295
  • Peer Reviewed
• [Journal Article] Identification of Additional Quantitative Trait Loci(QTL)Responsiblefor Susceptibility to Infectious Pancreatic Necrosis Virus in Rainbow Trout (2007)
  • Author(s): A. Ozaki, et. al.
  • Journal Title: Fish Pathology 42(3) Pages: 131-140
  • Peer Reviewed
• [Journal Article] A set of polymorphic tri-and tetra-nucleotide microsatellite markersfor the Japanese flounder(Paralichthys olivaceus) (2007)
  • Author(s): C.C.Sanchez et.al.
  • Journal Title: Animal Genetics 38 Pages: 75-76
  • Peer Reviewed
• [Journal Article] Characterization of novel microsatellite DNA markers in ayu, Plecoglossus altivelis (2006)
  • Author(s): M. Hara, T. Sakamoto, M. Sekino, K. Ohara, H. Matsuda, M. Kobayashi, and N. Taniguchi
  • Journal Title: Fisheries Science 72(1) Pages: 208-210
  • NAID: 10016736777
  • Peer Reviewed
• [Journal Article] Susceptibility and immune response to Flavobacterium psychrophilum between different stocks of Ayu Plecoglossus altivelis (2006)
  • Author(s): T. Nagai and T. Sakamoto
  • Journal Title: Fish Pathology 41(3) Pages: 99-104
  • NAID: 10020355466
  • Peer Reviewed
• [Journal Article] Characterization of novel microsatellite DNA markers in ayu, Plecoglossus altivelis. (2006)
  • Author(s): M.Hara et al.
  • Journal Title: Fisheries Science 72(1) Pages: 208-210
• [Journal Article] Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus). (2006)
  • Author(s): K.Fuji et al.
  • Journal Title: Aquaculture 254 Pages: 203-210
• [Journal Article] A linkage map for brown trout (Salmo trutta) : chromosome homeologies and comparative genome organization with other salmonid fish. (2006)
  • Author(s): K.Gharbi et al.
  • Journal Title: Genetics 172(4) Pages: 2405-2419
• [Journal Article] Six polymorphic microsatellite loci in the Japanese Spanish mackerel, Scomberomorus niphonius. (2006)
  • Author(s): E.Yokoyama et al.
  • Journal Title: Molecular Ecology Note 6 Pages: 323-324
• [Journal Article] Isolation of application of microsatellite DNA markers for pedigree tracing of seedings of red sea bream (Pagrus major). (2006)
  • Author(s): A.Hatanaka et al.
  • Journal Title: Journal of World Aquaculture Society 37(1) Pages: 139-143
• [Journal Article] Susceptibility and immune response to Flavobacterium psychrophilum between different stocks of Ayu Plecoglossus altivelis. (2006)
  • Author(s): T.Nagai et al.
  • Journal Title: Fish Pathology 41(3) Pages: 99-104
• [Presentation] 水産分野における耐病性形質のマーカー選抜育種研究 (2009)
  • Author(s): 坂本崇
  • Organizer: 日本動物遺伝育種学会
  • Place of Presentation: 岡山市、岡山県
  • Year and Date: 2009-11-30
• [Presentation] Genome Research in Japanese Aquaculture species (2009)
  • Author(s): T. Sakamoto
  • Organizer: Fish Genome Meeting 2009
  • Place of Presentation: Hinxton, UK
  • Year and Date: 2009-03-14
• [Presentation] Marker-assisted breeding of a bacterial coldwater disease resistant ayu (Plecoglossus altivelis) (2008)
  • Author(s): T. Sakamoto, 他4名
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-22
• [Presentation] Marker-assisted breeding of a bacterial coldwater disease resistant ayu (Plecoglossus altivelis) (2008)
  • Author(s): Takashi Sakamoto, 他4名
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-10-22
• [Presentation] Construction of genetic linkage map and identification of disease resistance locus against bacterial coldwater disease in ayu (Plecoglossus altivelis) (2008)
  • Author(s): Takashi Sakamoto, 他9名
  • Organizer: 5th International Symposium of the Japanese Society for Fish Pathology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-19
• [Presentation] Identification of disease resistance locus to bacterial coldwater disease in ayu (Plecoglossus altivelis) (2008)
  • Author(s): T. Sakamoto, 他4名
  • Organizer: The Seventh Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-10-09
• [Presentation] アユ冷水病耐性形質のマーカー選抜育種 (2007)
  • Author(s): 森本昌平, 他2名、坂本崇
  • Organizer: 平成19年度日本動物遺伝育種学会大会
  • Place of Presentation: 筑波、茨城
  • Year and Date: 2007-11-24
• [Presentation] アユ冷水病耐性形質のマーカー選抜育種 (2007)
  • Author(s): 森本昌平, 他4名
  • Organizer: 平成19年度日本動物遺伝育種学会大会
  • Place of Presentation: つくば
  • Year and Date: 2007-11-24
• [Book] 水産学シリーズ「ブリの資源培養と養殖業の展望」 (2006)
  • Author(s): 坂本 崇(分担執筆)
  • Total Pages: 135
  • Publisher: 恒星社厚生閣
• [Patent(Industrial Property Rights)] 冷水病耐性形質を有するアユの判別法、及び、これに用いるポリメラーゼ連鎖反応用プライマー (2007)
  • Inventor(s): 坂本崇, 他2名
  • Industrial Property Rights Holder: 東京海洋大学, 他1機関
  • Industrial Property Number: 2007-031978
  • Filing Date: 2007-02-13
• [Patent(Industrial Property Rights)] 冷水病耐性形質を有するアユの判別法、及び、これに用いるポリメラーゼ連鎖反応用プライマー (2007)
  • Inventor(s): 坂本崇 他2名
  • Industrial Property Rights Holder: 東京海洋大学 他1機関
  • Industrial Property Number: 2007-031978
  • Filing Date: 2007-02-13
• [Patent(Industrial Property Rights)] 冷水病耐性形質を有するアユの判別法、及び、これに用いるポリメラーゼ連鎖反応用プライマー (2006)
  • Inventor(s): 坂本崇, 他2名
  • Industrial Property Rights Holder: 東京海洋大学, 他1機関
  • Industrial Property Number: 2006-314364
  • Filing Date: 2006-11-21
• [Patent(Industrial Property Rights)] 冷水病耐性形質を有するアユの判別法、及び、これに用いるポリメラーゼ連鎖反応用プライマー (2006)
  • Inventor(s): 坂本崇 他2名
  • Industrial Property Rights Holder: 東京海洋大学 他1機関
  • Industrial Property Number: 2006-314364
  • Filing Date: 2006-11-21