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ブタ卵成熟過程におけるヒアルロン酸-CD44のCx43調節機構の解析

Research Project

Project/Area Number 18780209
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

横尾 正樹  Tohoku University, 先進医工学研究機構, 助教 (10396541)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsブタ / 卵成熟 / 卵丘膨化 / ヒアルロン酸 / ギャップ結合 / ギャップジャンクション / CD44 / Cx43 / 卵子
Research Abstract

申請者によるこれまでの研究から,ブタ卵成熟過程において卵丘膨化時の分泌されるヒアルロン酸が卵成熟誘起に重要な役割を果たしていること,さらにその作用が卵丘細胞で発現するヒアルロン酸受容体(CD44)を介して機能していることを明らかにしている。本研究では,ブタ卵成熟誘起機構におけるヒアルロン酸-CD44作用について解析し,以下のような減数分裂再開における一連の作用機序を明らかにした。
卵丘膨化に伴うヒアルロン酸-CD44作用が,卵丘細胞にLHレセプターを発現させる。(ヒアルロン酸-CD44作用がなければ,LHレセプターが発現しない)
LHの作用によってコネキシン43のチロシンリン酸化が誘導され,卵丘細胞-卵子複合体におけるギャップ結合を閉鎖させる。
卵丘細胞から卵子へのcAMP流入が阻害され,卵子内cAMP濃度低下に伴いMPFが活性化される。
その結果,卵子の減数分裂が再開される。
これらの研究成果は,より高品質なブタ成熟卵子を安定的に供給できる培養システムの開発に向け,重要な知見である。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (9 results)

All 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] Role of the Hyaluronan Receptor CD44 during Porcine Oocyte Maturation.2007

    • Author(s)
      M. Yokoo, et. al.
    • Journal Title

      Journal of Reproduction and Development 53

      Pages: 263-270

    • NAID

      10027327814

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory action of FSH in apoptosis of swine granulosa cells in COCG culture involves hyaluronan synthesis in an intrinsic Pathway.2007

    • Author(s)
      Sri Tunjung WA, et. al.
    • Journal Title

      Biology of Reproduction 77

      Pages: 226-226

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of mitochondrial function in single cloned miniature pig embryos by measuring oxygen consumption.2007

    • Author(s)
      S.Sugimura et al.
    • Journal Title

      Reproduction, Fertility and Development. 19

      Pages: 161-161

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The efficient maintenance of oxygen consumption of porcine oocytes relates to the high competence of oocyte maturation2007

    • Author(s)
      M.Yokoo et al.
    • Journal Title

      Reproduction, Fertility and Development. 19

      Pages: 215-215

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role of hyaluronan receptor CD44 during porcine oocyte maturation.2007

    • Author(s)
      M.Yokoo et al.
    • Journal Title

      Journal of Reproduction and Development 53(in press)

    • NAID

      10027327814

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ブタ卵成熟機構における卵丘膨化の役割2006

    • Author(s)
      横尾 正樹
    • Journal Title

      豚の繁殖衛生セミナー通信 33

      Pages: 30-34

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Morphodynamics of ovarian follicles during oogenesis in mice.2006

    • Author(s)
      E.Sato et al.
    • Journal Title

      Microscopy Research and Technique. 69

      Pages: 427-435

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression and Glycosylation with Polylactosamine of CD44 Antigen on Macrophages During Follicular Atresia in Pig Ovaries.2006

    • Author(s)
      Y.Miyake et al.
    • Journal Title

      Biology of Reproduction. 74

      Pages: 501-510

    • Related Report
      2006 Annual Research Report
  • [Presentation] Inhibitory action of FSH in apoptosis of swine granulosa cells in COCG culture involves hyaluronan synthesis in an intrinsic pathway.2007

    • Author(s)
      Sri Tunjung WA, et. al.
    • Organizer
      40th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      San Antonio, Texas
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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