Understanding plant freezing tolerance mechanism through physiological and biochemical analyses at freezing temperatures

• Project/Area Number: 18780242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: Iwate University
• Principal Investigator: KAWAMURA Yukio Iwate University, 農学部, 准教授 (10400186)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 植物 / 耐凍性 / 凍結下 / 生理生化学的解析 / 凍結耐性 / シロイヌナズナ / 細胞膜 / 内膜 / 細胞膜修復 / SNARE / シナプトタグミン / プロトプラスト / 組織切片

Research Abstract

凍結下でおきる膜のダイナミクスは、植物が高い耐凍性を得るための必須条件と考えられる。本研究では、凍結過程における細胞膜と内膜の変化を、生理・生化学的手法により解析し、凍結、もしくは凍結融解過程における膜のダイナミクスをとらえることに成功した。また、このダイナミクスの一つである細胞膜修復に、植物シナプトタグミンSYT1 が関与することを示し、さらに、SNARE タンパク質も関与する可能性を示す結果を得た。

Research Products

• [Journal Article] Extracellular freezing-induced mechanical stress and surface area regulation on the plasma membrane in cold-acclimated plant cells (2009)
  • Author(s): Yamazaki, T.、Kawamura, Y.、Uemura, M.
  • Journal Title: Plant Signaling and Behavior
  • Peer Reviewed
• [Journal Article] Calcium-dependent freezing tolerance in Arabidopsis involves membrane resealing via synaptotagmin SYT1 (2008)
  • Author(s): Yamazaki, T.、Kawamura, Y.、Minami, A.、Uemura, M.
  • Journal Title: Plant Cell 20 Pages: 3389-3404
  • Peer Reviewed
• [Journal Article] Cryobehavior of the plasma membrane in protoplasts isolated from cold-acclimated Arabidopsis leaves is related to surface area regulation (2008)
  • Author(s): Yamazaki, T.、Kawamura, Y.、Uemura, M.
  • Journal Title: Plant Cell Physiology 49 Pages: 944-957
  • NAID: 10027331922
  • Peer Reviewed
• [Journal Article] Cryobehavior of the plasma membrane in protoplasts isolated from cold-acclimated Arabidopsis leaves is related to surface area regula tion (2008)
  • Author(s): Yamazaki T, Kawamura Y, Uemura M
  • Journal Title: Plant and Cell Physiolgy 49 Pages: 944-957
  • Peer Reviewed
• [Journal Article] Calcium-dependent freezing tolerance in Arabidopsis involves memb rane resealina via svnantotaarnin SYT1 (2008)
  • Author(s): Yamazaki T, Kawamura Y, Min ami A, Uemura M
  • Journal Title: Plant Cell 20 Pages: 3389-3404
  • Peer Reviewed
• [Journal Article] 凍結における機械的ストレスとその耐性機構 (2006)
  • Author(s): 河村幸男、山崎誠和、上村松生
  • Journal Title: 低温生物工学会誌 52 Pages: 169-173
  • Peer Reviewed
• [Journal Article] 凍結における機械的ストレスとその耐性機構 (2006)
  • Author(s): 河村幸男, 山崎誠和, 上村松生
  • Journal Title: 低温生物工学会誌 52 Pages: 169-173
• [Presentation] A plant synaptotagmin is involved in freezing tolerance (2008)
  • Author(s): Yamazaki T. 、Kawamura Y.vMinami A. 、Uemura M.
  • Organizer: XVI Congress of the Federation of European Societies of Plant Biology (FESPB 2008)
  • Place of Presentation: Tampere、Finland
• [Presentation] A plant synaptotagmin is involved in freezing tolerance (2008)
  • Author(s): Yamazaki T, Kawamura Y, Min ami A, Uemura M
  • Organizer: XVI Congress of the Federation of European Societies of Plant Bioloav
  • Place of Presentation: Finland (Tampere)
• [Presentation] 氷晶成長による機械的ストレスとその耐性機構 (2007)
  • Author(s): 河村幸男、山崎誠和、上村松生
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 松山
• [Presentation] Enhanced freezing tolerance is dependent on the extracellular calcium during freezing (2006)
  • Author(s): Kawamura Y., Yamazaki T., Uemura M.
  • Organizer: Plant Biology 2006
  • Place of Presentation: Boston, USA
• [Presentation] 凍結における機械的ストレスとその耐性機構 (2006)
  • Author(s): 河村幸男、山崎誠和、上村松生
  • Organizer: 第52回低温生物工学会大会
  • Place of Presentation: 福岡
• [Remarks] ホームページ等
  • URL: http://news7a1.atm.iwate-u.ac.jp/~crcdbbt/index.htm