X-ray crystallographic analysis of DNA using temperature-control crystallization technique

• Project/Area Number: 18790030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: CHATAKE Toshiyuki Kyoto University, 原子炉実験所, 准教授 (30383475)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 物理化学 / 核酸 / 薬剤 / 放射線・X線・粒子線 / 結晶化 / 薬学 / 結晶成長 / 金属と生体分子 / 水和構造 / 放射線・X点・粒子線

Research Abstract

本研究の目的は、独自に開発した温度制御による結晶化法を用いることによりDNA のX 線結晶解析の効率を飛躍的に向上させ、構造に基づいた核酸系薬剤のドラッグデザインへの基盤を確立することにある。今回の研究では、(1)温度制御結晶化法を結晶成長学的な観点から実証して、(2)これに基づいたB 型・Z 型DNA の結晶化と高分解能X 線結晶構造解析を行い、(3)薬剤設計の基本となる特殊なDNA (デオキシ型リボザイム・ミニヘアピンDNA・ボロン化合物を導入したDNA)の結晶化を行った。

Research Products

• [Journal Article] Functional aberration of myofibrils by cardiomyopathy-causing mutations in the coiled-coil region of the troponin-core domain (2009)
  • Author(s): Matsumoto F, Maeda K, Chatake T, Maeda Y, Fujiwara S
  • Journal Title: Biochem BioPhys Research Communication 382 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Functional aberration of myofibrils by cardiomyopathy-causing mutations in the coiled-coil region of the trononin-core domain (2009)
  • Author(s): Matsumoto F, Maeda K, Chatake T, Maeda Y, Fujiwara S
  • Journal Title: Biochem Biophys Research Communication 382 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Hydrogen bonds of DsrD protein revealed by neutron crystallography (2008)
  • Author(s): Chatake T, Higuchi Y, Mizuno N, Tanaka I, Niimura N, Morimoto Y
  • Journal Title: Journal of Synchrotron Radiation 15 Pages: 277-280
  • Peer Reviewed
• [Journal Article] An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis (2008)
  • Author(s): Ishikawa T, Chatake T, Morimoto Y, Maeda M, Kurihara K, Tanaka I, Niimura N
  • Journal Title: Biochem BioPhys Research Communication 376 Pages: 32-35
  • Peer Reviewed
• [Journal Article] Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin (2008)
  • Author(s): Kovalevsky AY, Chatake T, Shibayama N, Park SY, Ishikawa T, Mustyakimov M, Fisher SZ, Langan P, Morimoto Y
  • Journal Title: Acta Crystallogr F64 Pages: 270-273
  • Peer Reviewed
• [Journal Article] Hydrogen bonds of DsrD protein revealed by neutron crystallograph (2008)
  • Author(s): Chatake T, Higuchi Y, Mizuno N, Tanaka I, Niimura N, Morimoto Y
  • Journal Title: Journal of Synchrotron Radiation 15 Pages: 277-280
  • Peer Reviewed
• [Journal Article] An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis (2008)
  • Author(s): Ishikawa T, Chatake T, Morimoto Y, Maeda M, Kurihara K, Tanaka I, Niimura N
  • Journal Title: Biochem Biophys Research Communication 376 Pages: 32-35
  • Peer Reviewed
• [Journal Article] Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin. (2008)
  • Author(s): Kovalevsky, Chatake, Shibayama, Park, Ishikawa, Mustyakimov, Fisher, Langan, Morimoto.
  • Journal Title: Acta Crystallographica Section F 64 Pages: 270-273
  • Peer Reviewed
• [Journal Article] Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography (2007)
  • Author(s): Chatake T, Shibayama N, Park SY, Kurihara K, Tamada T, Tanaka I, Niimura N, Kuroki R, Morimoto Y
  • Journal Title: Journal of American Chemical Society 129 Pages: 14840-14841
  • Peer Reviewed
• [Journal Article] Protocol of refinement in neutron crystallographic analysis (2007)
  • Author(s): Chatake T, Morimoto Y
  • Journal Title: 日本中性子科学会誌 17 Pages: 216-217
  • NAID: 130001998320
• [Journal Article] Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography. (2007)
  • Author(s): Chatake, Shibayama, Park, Kurihara, Tamada, Tanaka, Niimura, Kuroki, Morimoto.
  • Journal Title: Journal of American Chemical Society 129 Pages: 14840-14841
  • Peer Reviewed
• [Journal Article] Protocol of refinement in neutron crystallographic analysis. (2007)
  • Author(s): Chatake, Morimoto
  • Journal Title: 中性子科学会誌「波紋」 17 Pages: 216-217
  • NAID: 130001998320
• [Presentation] (2009)
  • Author(s): 茶竹俊行
  • Organizer: 温度制御によるDNA の結晶化とその応用タンパク質結晶育成研究会
  • Place of Presentation: 東海村(茨城県)
  • Year and Date: 2009-03-06
• [Presentation] 温度制御によるDNAの結晶化とその応用 (2009)
  • Author(s): 茶竹俊行
  • Organizer: タンパク質結晶育成研究会
  • Place of Presentation: 東海村(茨城県)
  • Year and Date: 2009-03-06
• [Presentation] (2008)
  • Author(s): Chatake, Ishikawa, Morimoto
  • Organizer: Crystal Structures of Z-DNA complexed with Ca2+ ion, and Mg2+ ion XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: 大阪市(大阪府)
  • Year and Date: 2008-08-25
• [Presentation] Crystal Structures of Z-DNA complexed with Ca2+ ion, and Mg2+ ion (2008)
  • Author(s): Chatake T., Ishikawa T., Morimoto Y
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: 大阪府
  • Year and Date: 2008-08-25
• [Presentation] 金属イオンがDNA構造に与える影響 (2007)
  • Author(s): 石川卓哉・森本幸生・茶竹俊行
  • Organizer: 日本結晶学会年会
  • Place of Presentation: 東京都
  • Year and Date: 2007-12-02
• [Presentation] 金属イオンがDNA構造に与える影響 (2007)
  • Author(s): 石川卓哉・森本幸生・茶竹俊行
  • Organizer: 日本結晶学会
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2007-12-02
• [Presentation] 蛋白質・DNAの中性子構造生物学による展望 (2007)
  • Author(s): 茶竹俊行・森本幸生
  • Organizer: 169委員会講演会
  • Place of Presentation: 茨城県東海村
  • Year and Date: 2007-10-25