Search for an analgesic drug based on analysis of NCAM role in neuropathic pain

• Project/Area Number: 18790184
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Nippon Medical School
• Principal Investigator: SAKAI Atsushi Nippon Medical School, 医学部, 助教 (30386156)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 神経因性疼痛 / GDNF / NCAM / DRG / レンチウイルスベクター / 脊髄

Research Abstract

神経因性疼痛に対して鎮痛作用を有するグリア細胞株由来神経栄養因子(GDNF)の作用標的候補として神経接着因子であるNCAMの解析を行い、脊髄後角においてNCAMを発現する細胞やNCAMを刺激する事で鎮痛効果が得られることを明らかにした。また、GDNF発現レンチウイルスベクターを利用することにより疼痛発症部位に特異的且つ長期的に痛みを取り除くことができる事を示した。

Research Products

• [Journal Article] Increase in hemokinin-1 mRNA in the spinal cord during the early phase of a neuropathic pain state (2008)
  • Author(s): Matsumura T, Sakai A, Nagano M, Sawada M, Suzuki H, Umino M, Suzuki H
  • Journal Title: British Journal of Pharmacology 155 Pages: 767-774
  • Peer Reviewed
• [Journal Article] NCAM as a target for GDNF-induced analgesia in neuropathic pain (2008)
  • Author(s): Sakai A, Suzuki H
  • Journal Title: Journal of Nippon Medical School 75 Pages: 136-137
  • NAID: 130004057327
  • Peer Reviewed
• [Journal Article] Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain (2008)
  • Author(s): Sakai A, Asada M, Seno N, Suzuki H
  • Journal Title: Pain 137 Pages: 378-388
  • Peer Reviewed
• [Journal Article] The prolonged analgesic effect of epidural ropivacaine in a rat model of neuropathic pain (2008)
  • Author(s): Sato C, Sakai A, Ikeda Y, Suzuki H, Sakamoto A
  • Journal Title: Anesthesia & Analgesia 106 Pages: 313-320
  • Peer Reviewed
• [Journal Article] NCAM as a target for GDNF-induced analgesia in neuropathic pain (2008)
  • Author(s): Atsushi Sakai
  • Journal Title: Journal of Nippon Medical School 75 Pages: 136-137
  • NAID: 130004057327
  • Peer Reviewed
• [Journal Article] Increase in hemokinin-1 mRNA in the spinal cord during the early phase of a neuropathic pain state (2008)
  • Author(s): Tomoka Matsumura
  • Journal Title: British Journal of Pharmacology 155 Pages: 767-774
  • Peer Reviewed
• [Journal Article] The prolonged analgesic effect of epidural ropivacaine in a rat model of neuropathic pain (2008)
  • Author(s): Chiyo Sato
  • Journal Title: Anesthesia & Analgesia 106 Pages: 313-320
  • Peer Reviewed
• [Journal Article] Expression changes of cation chloride cotransporters in the rat spinal cord following intraplantar formalin (2006)
  • Author(s): Nomura H, Sakai A, Nagano M, Umino M, Suzuki H
  • Journal Title: Neuroscience Research 56 Pages: 435-440
  • NAID: 10019273651
  • Peer Reviewed
• [Journal Article] Expression changes of cation chloride cotransporters in the rat spinal cord following intraplantar formalin (2006)
  • Author(s): Hidehiko Nomura
  • Journal Title: Neuroscience Research 56・4 Pages: 435-440
• [Journal Article] Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain
  • Author(s): Atsushi Sakai
  • Journal Title: Pain (in press)
  • Peer Reviewed
• [Presentation] Overexpression of GDNF in uninjured DRG neurons exhibits an analgesic effect in a rat neuropathic pain model (2008)
  • Author(s): Atsushi Sakai
  • Organizer: 6th FENS Forum of European Neuroscience
  • Place of Presentation: Geneva Palexpo
  • Year and Date: 2008-07-13
• [Presentation] Overexpression of GDNF in uninjured DRG neurons exhibits an analgesic effect in a rat neuropathic pain model (2008)
  • Author(s): Atsushi Sakai
  • Organizer: 6^<th> FENS Forum of European Neuroscience
  • Place of Presentation: Geneva Palexpo
  • Year and Date: 2008-07-13
• [Presentation] 新規タキキニンhemokinin-1の神経因性疼痛モデル動物における発現の増加. (2008)
  • Author(s): 松村朋香
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-18
• [Presentation] 新規タキキニンhemokinin-1の神経因性疼痛モデル動物における発現の増加 (2008)
  • Author(s): 松村朋香
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-18
• [Presentation] NCAM is involved in GDNF-induced analgesia in neuropathic pain (2007)
  • Author(s): Atsushi Sakai
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne Convention Centre.
  • Year and Date: 2007-07-13
• [Presentation] NCAM is involved in GDNF-induced analgesia in neuropathic pain (2007)
  • Author(s): Atsushi Sakai
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne Convention Centre
  • Year and Date: 2007-07-13
• [Presentation] 神経因性疼痛時におけるDRGニューロンでのstathminの発現上昇 (2007)
  • Author(s): 高須久望子
  • Organizer: 第80回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2007-03-16
• [Presentation] 神経因性疼痛の発症維持におけるNCAMの関与 (2007)
  • Author(s): 坂井敦
  • Organizer: 第80回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2007-03-14
• [Presentation] GDNFの神経因性疼痛の緩和作用に対するNCAMの関与 (2006)
  • Author(s): 坂井敦
  • Organizer: 第115回日本薬理学会関東部会、第34回薬物活性シンポジウム
  • Place of Presentation: 高崎シティギャラリー高崎市庁舎会議室.
  • Year and Date: 2006-09-28
• [Presentation] NCAM is involved in the analgesic effect of GDNF on the neuropathic pain (2006)
  • Author(s): 坂井敦
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2006-07-20