Development of the efficient method to purify and obtain mesenchymal progenitors derived from human amniotic membrane

• Project/Area Number: 18790211
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUDO Kazuhiro The Institute of Physical and Chemical Research, 細胞材料開発室, 協力研究員 (10392002)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 体性幹細胞 / 胎児付属組織 / 羊膜 / 間葉系幹細胞 / 分化能 / ヒト羊膜 / マーカー / 間葉系幹 / 前駆細胞 / 純化 / 胎児付随組織

Research Abstract

ヒト羊膜中に骨芽細胞、軟骨、脂肪細胞に分化可能な間葉系幹/前駆細胞が存在していることを明らかにし、さらにこれらの細胞は羊膜中においてCD90またはCD166陽性細胞であることが示唆された。しかし、分離、培養後数継代を経た細胞は分化能を有するか否かに関わらず全てCD90およびCD166陽性であり、これらの細胞表面抗原の発現がin vitroでの分化能を直接反映していないことが明らかになった。

Research Products

• [Journal Article] Mesenchymal Progenitors Able to Differentiate into Osteogeic, Chondrogenic, and/or Adipogenic Cells in vitro are present in Most Primary Fibloblast-like Cell Populations (2007)
  • Author(s): Kazuhiro Sudo
  • Journal Title: Stem Cells 25 Pages: 1610-7
  • Peer Reviewed
• [Presentation] Mesenchymal Progenitors Able to Differentiate into Osteogeic, Chondrogenic, and/or Adipogenic Cells in vitro are present in Most Primary Fibloblast-like Cell Populations (2008)
  • Author(s): Kazuhiro Sudo
  • Organizer: The 2008 World congress on in vitro biology (Invited speaker)
  • Place of Presentation: Tuscon, Arizona, USA
  • Year and Date: 2008-06-17
• [Presentation] Mesnchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or aduoigenic cells in vitro are present in most primary fibroblast-like cell populations. (2008)
  • Author(s): Kazuhiro Sudo
  • Organizer: World congress on in vitro biology
  • Place of Presentation: Tsuson, Arizona
  • Year and Date: 2008-06-17
• [Presentation] ヒト羊膜由来間葉系前駆細胞の造血支持能の検討 (2008)
  • Author(s): 須藤和寛
  • Organizer: 日本組織培養学会第81回大会
  • Place of Presentation: つくば、茨城
  • Year and Date: 2008-05-20
• [Presentation] ヒト線維芽細胞の分化能の検討-2 (2007)
  • Author(s): 須藤和寛
  • Organizer: 日本組織培養学会第80回大会
  • Place of Presentation: 大阪
  • Year and Date: 2007-05-15
• [Presentation] ヒト線維芽細胞の分化能に基づく分類 (2007)
  • Author(s): 須藤和寛
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-03-13
• [Presentation] ヒト線維芽細胞の分化能の検討 (2006)
  • Author(s): 須藤和寛
  • Organizer: 日本組織培養学会第79回大会
  • Place of Presentation: 東京
  • Year and Date: 2006-05-26