The role of mcl-1 in pancreatic β-cell during differentiation and high glucose stimulation

• Project/Area Number: 18790282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Nihon University
• Principal Investigator: SANO Makoto Nihon University, 医学部, 医学部 (70339323)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 膵ラ氏島 / β細胞 / Mcl-1遺伝子 / インスリン産生細胞 / AR42J細胞 / 糖負荷 / Bcl-2ファミリー遺伝子 / nesidioblastosis / インスリン産生

Research Abstract

糖尿病には,膵ラ氏島のインスリン産生細胞(β細胞)が深く関わっている.本研究では,インスリン産生細胞の分化誘導モデルとしてAR42J細胞を用い,β細胞の分化過程と糖負荷刺激におけるアポトーシス抑制遺伝子Mcl-1の動態を解析した.その結果,β細胞への分化過程,ならびに高濃度の糖負荷刺激に伴って誘導されるアポトーシスの抑制に,Mcl-1遺伝子の発現が密接に関わっていることが明らかとなった.

Research Products

• [Journal Article] Mcl-1, an anti-apoptotic Bcl-2 family member, essentially overlaps with insulin- producing cells in neonatal nesidioblastosis (2008)
  • Author(s): Sano M, Hayashi E, Murakami H, Kishimoto H, Fukuzawa R, and Nemoto N
  • Journal Title: Virchows Archiv Pages: 452-452
• [Journal Article] Mcl-1, an anti-apoptotic Bcl-2 family member, essentially overlaps with insulin-producing cells in neonatal nesidioblastosis
  • Author(s): Sano M, Hayashi E, Murakami H, Kishimoto H, Fukuzawa R, and Nemoto N
  • Journal Title: Virchows Archiv (In press)
  • Peer Reviewed
• [Presentation] 小児膵島細胞症(nesidioblastosis)におけるMcl-1遺伝子の発現分布 (2007)
  • Author(s): 佐野 誠,林 恵美子,村上仁彦,岸本宏志,福澤龍二,根本則道
  • Organizer: 第48回日本組織細胞化学会総会・第39回日本臨床分子形態学会総会合同学術集会
  • Place of Presentation: 甲府
• [Book] Testicular Cancer Research Trends ; Expression of Bcl-2 family members in testicular germ cell tumors,Chapter II (2007)
  • Author(s): Sano M, Suzuki A, and Nemoto N
  • Publisher: Nova Science Publishers, Inc
• [Book] Testicular Cancer Research Trends : Expression of Bcl-2 family members in testicular germ cell tumors
  • Author(s): Sano M, Suzuki A, Nemoto N
  • Publisher: Nova Science Publishers, inc., NY(in press)