Research on the function of a novel adhesion factor LaiA and its paralog molecules that are related to virulence of Legionella pneumo

• Project/Area Number: 18790316
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: CHANG Bin National Institute of Infectious Diseases, 細菌第一部, 主任研究官 (50370961)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: レジオネラ / 細胞内寄生菌 / 接着因子 / サイトカイン / 免疫応答 / Legionella pneumophila / 生体防御機構 / 肺胞上皮細胞 / 生体防御応答 / 侵入経路 / 接着分子 / 伝播期 / エフェクター分子

Research Abstract

本研究はLegionella pneumophilaの接着因子LaiAおよびそのパラログ分子の、宿主への感染における役割と、L.pneumophila感染によって活性化された宿主細胞側の自然免疫応答機構の両面について進めてきた。まずLaiAおよびそのパラログ分子はL.pneumophilaの膜タンパクで、宿主細胞への接着や侵入に関与しているが、本菌の細胞内増殖には関与していないことを明らかにした。次ぎに宿主細胞側の自然免疫応答については、肺胞上皮細胞はL.pneumophila生菌の感染により細胞内シングナル伝達が活性化され、IL-6、IL-8、TNF-α、MCP-1などのサイトカインの発現・分泌が見られた。また、このシングナル伝達にはNOD1(Nucleotide-binding oligomerization domain containing 1)およびNOD2宿主細胞内分子が関与することを明らかにした。しかし、これらのシングナル伝達はLaiAおよびそのパラログ分子によっては活性化されなかった。

Research Products

• [Journal Article] Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR. (2009)
  • Author(s): B. Chang, K. Sugiyama, T. Taguri, J. Amemura-Maekawa, F. Kura, H. Watanabe.
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 147-153
• [Journal Article] An improved protocol for the preparation and restriction enzyme digestion of PFGE agarose plugs for the analysis of Legionella isolates (2009)
  • Author(s): B. Chang, J. Amemura-Maekawa, H. Watanabe
  • Journal Title: Japanese Journal of Infectious Diseases 62 Pages: 54-56
• [Journal Article] Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR (2009)
  • Author(s): Bin Chang, Kanji Sugiyama, Toshitsugu Taguri, Junko Amemura-Maekawa, Fumiaki Kura, and Haruo Watanabe
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 147-153
  • Peer Reviewed
• [Journal Article] An improved protocol for the preparation and restriction enzyme digestion of pulsed-field gel electrophoresis agarose plugs for the analysis of Legionella isolates (2009)
  • Author(s): Bin Chang, Junko Amemura-Maekawa, and Haruo Watanabe
  • Journal Title: Japanese Journal of Infectious Diseases 62 Pages: 54-56
  • NAID: 40016423812
  • Peer Reviewed
• [Journal Article] Distinct difference of flaA genotypes of Legionella pneumophila between isolates from bath water and cooling tower water. (2008)
  • Author(s): J. Amemura-Maekawa, F. Kura, B. Chang, A. Suziki-Hashimoto, M. Ichinose, H. Watanabe
  • Journal Title: Microbiology and Immunology 52 Pages: 460-464
  • NAID: 10025692234
• [Journal Article] レジオネラを解析するパルスフィールド・ゲル電気泳動(PFGE)法の改良 (2008)
  • Author(s): 常彬、前川純子、渡辺治雄
  • Journal Title: 病原微生物情報 29 Pages: 333-334
• [Journal Article] 神戸市における公衆浴場でのレジオネラ症集団発生事例 (2008)
  • Author(s): 木股裕子、南出正之、名正文、常彬、前川純子
  • Journal Title: 病原微生物情報 29 Pages: 329-330
• [Journal Article] Distinct difference of flaA genotypes of Legionella pneumophila between isolates from bath water and cooling tower water (2008)
  • Author(s): Junko Amemura-Maekawa, Fumiaki Kura, Bin Chang, Atsuko Suzuki-Hashimoto, Masayuki Ichinose, Takuro Endo, and Haruo Watanabe
  • Journal Title: Microbiology and Immunology 52 Pages: 460-464
  • NAID: 10025692234
  • Peer Reviewed
• [Journal Article] Pulsed-field gel electrophoresis (PFGE) analysis and sequence-base typing (SBT) of Legionella pneumophila serogroup 1 isolates from Japan. (2006)
  • Author(s): J. Amemura-Maekawa, F. Kura, B. Chang, H. Watanabe
  • Journal Title: Legionella(ASM Press) Pages: 159-162
• [Journal Article] Locus on chromosome 13 in mice involved in clearance of Legionella pneumophila from the lungs (2006)
  • Author(s): S. Kobayashi, F. Kura, J. Amemura-Maekawa, B. Chang, N. Yamamoto, H. Watanabe
  • Journal Title: Legionella(ASM Press) Pages: 310-312
• [Journal Article] Surveillance of group B streptococcal toxic shock-like syndrome in nonpregnant adults and characterization of the strains in Japan (2006)
  • Author(s): Bin Chang
  • Journal Title: Japanese Journal of Infectious Diseases 59・3 Pages: 4-4
  • NAID: 40007307410
• [Journal Article] Characteristics of Streptococcus suis isolated from patients in Japan (2006)
  • Author(s): Bin Chang
  • Journal Title: Japanese Journal of Infectious Diseases 59・6 Pages: 3-3
  • NAID: 40015197548
• [Presentation] レジオネラ解析用パルスフィールド電気泳動(PFGE)法の改良 (2009)
  • Author(s): 常彬, 前川純子, 渡辺治雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-12
• [Presentation] レジオネラ解析用パルスフィールド電気泳動(PFGE)法の改良 (2009)
  • Author(s): 常彬、前川純子、渡辺治雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Legionella pneumophila血清群1分離株の遺伝子型別およびモノクローナル抗体型別 (2009)
  • Author(s): 前川純子、倉文明、常彬、市瀬正之、渡辺治雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Sequence based typing and monoclonal antibody typing of Legionella pneumophila serogroup 1 clinical and environmental isolates in Japan. (2008)
  • Author(s): J. Amemura-Maekawa, F. Kura, B. Chang, H. Watanabe
  • Organizer: The 23rd Annual Meeting of the European Working Group for Legionella Infections
  • Place of Presentation: Madrid, Spain
• [Presentation] Discrimination of viable and dead Legionella cells by a combined use of photoactivated ethidium monoazide and PCR/real-time PCR. (2008)
  • Author(s): B. Chang, N. Honda, S-I. Yoshida, J. Amemura-Maekawa, F. Kura, H. Watanabe
  • Organizer: XII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Istanbul, Turkey
• [Presentation] The surveillance of Legionella from cooling towers between 2001 and 2006 in Japan (2007)
  • Author(s): Atsuko Suzuki-Hashimoto
  • Organizer: The 22nd Annual Meeting of the European Working Group for Legionella Infections
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2007-06-03
• [Presentation] Surveillance of Leglonella isolates from bathtub water in Japan : an increase of the rate of Legionella pneumophila serogroup 1 (2007)
  • Author(s): Fumiaki Kura
  • Organizer: The 22nd Annual Meeting of the European Working Group for Legionella Infections
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2007-06-03
• [Presentation] The surveillance of Legionella from cooling towers between 2001 and 2006 in Japan. (2007)
  • Author(s): A. Suzuki-Hashimoto, J. Amemura-Maekawa, F. Kura, B. Chang, S. Izumiyama, M. Ichinose, H. Watanabe, T. Endo
  • Organizer: The 22nd Annual Meeting of the European Working Group for Legionella Infections Stockholm
  • Place of Presentation: Sweden
• [Presentation] Surveillance of Legionella isolates from bathtub water in Japan: an increase of the rate of Legionella pneumophila serogroup 1. (2007)
  • Author(s): F. Kura, J. Amemura-Maekawa, A. Suzuki-Hashimoto, B. Chang, S. Izumiyama, M. Ichinose, T. Endo, H. Watanabe
  • Organizer: The 22nd Annual Meeting of the European Working Group for Legionella Infections. Stockholm
  • Place of Presentation: Sweden
• [Presentation] Legionella pneumophilaのモノクローナル抗体を用いたドレスデンパネルによる分類 (2007)
  • Author(s): 前川純子、倉文明、常彬、渡辺治雄
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: 大阪
• [Presentation] 浴槽水からのレジオネラ属菌の検出状況-Legionella pneumophila血清群1の増加- (2007)
  • Author(s): 倉文明、前川純子、鈴木敦子、常彬、泉山信司、市瀬正之、遠藤卓郎、渡辺治雄
  • Organizer: 第81回日本感染症学会総会
  • Place of Presentation: 京都
• [Presentation] 冷却塔水からのレジオネラ属菌の検出状況-2001年度から2006年度まで- (2007)
  • Author(s): 鈴木敦子、前川純子、倉文明、常彬、泉山信司、市瀬正之、渡辺治雄、遠藤卓郎
  • Organizer: 第81回日本感染症学会総会
  • Place of Presentation: 京都
• [Presentation] Typing of Legionella peumophila Isolates in Japan by flaA Gene. (2006)
  • Author(s): J. Amemura-Maekawa, F. Kura, B. Chang, A. Suzuki-Hashimoto, M. Ichinose, H. Watanabe
  • Organizer: The 21st Annual Meeting of the European Working Group for Legionella Infections Lisbon
  • Place of Presentation: Portugal
• [Presentation] 新しい分子疫学的手法であるsequence-based typing (SBT)によるLegionella pneumophila血清群1の臨床および環境分離株の型別 (2006)
  • Author(s): 前川純子, 倉文明, 常彬, 渡辺治雄
  • Organizer: 第80回日本感染症学会総会
  • Place of Presentation: 東京
• [Book] レジオネラ感染症ハンドブック(斎藤厚 編), レジオネラ感染の分子機構) (2007)
  • Author(s): 常彬、渡辺治雄
  • Publisher: 日本医事新報社
• [Book] レジオネラ感染症ハンドブック (2007)
  • Author(s): 常 彬
  • Total Pages: 10
  • Publisher: 日本医事新報社
• [Book] レジオネラ(荒川宜親、渡辺治雄監修、佐々木次雄(編集), 図説 呼吸器系細菌感染症:疫学、診断、治療) (2006)
  • Author(s): 倉文明、常彬、前川純子
  • Publisher: じほう
• [Book] 図説 呼吸器系細菌感染症 疫学・診断・治療 (2006)
  • Author(s): 倉 文明
  • Total Pages: 18
  • Publisher: じほう
• [Patent(Industrial Property Rights)] レジオネラ属菌の検出方法及び検出キット
  • Inventor(s): 常彬、前川純子、倉文明、渡辺治雄、吉田真一、副島隆志