The epigenetic regulation in infantile epilepsy

• Project/Area Number: 18790707
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: University of Yamanashi
• Principal Investigator: HIRASAWA Takae University of Yamanashi, 大学院医学工学総合研究部, 助教 (10402083)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥5,480,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: てんかん / エピジェネティクス / 遺伝子発現調節 / 小児性てんかん / 抑制性 / 発現制御

Research Abstract

小児性てんかん患者が遺伝子変異ではなく発現調節異常によるものと想定し、研究をスタートさせた。てんかんと関係がある神経栄養因子や、DNAメチル化調節試薬による標的分子発現変化を検討した。DNAの遺伝子調節を検討するため、DNAメチル化結合蛋白質のノックアウトマウスにおける標的分子の発現変化を確認した。本研究ではてんかんの症状に神経栄養因子が関わっている点、またDNAメチル化結合蛋白と標的分子の発現変化はてんかんとの新たな関連性も示唆した。今後クロマチン免疫沈降法等でさらに詳細なデータを収集する予定である。

Research Products

• [Journal Article] Transthyretin accelerates vascular Abeta deposition in a mouse model of alzheimer's disease (2009)
  • Author(s): Wati H, Kawarabayashi T, Matsubara E, Kasai A, Hirasawa T, Kubota T, Hagiwara Y, Shogi M, Maeda S
  • Journal Title: Brain Pathol 19(1) Pages: 48-57
  • Peer Reviewed
• [Journal Article] Augmentation of plysialic acid by valproic acid in early postnatal mouse hippocampus and primary cultured hippocampal neurons (2008)
  • Author(s): Natori T, Kodaira F, Hirasawa T, Gao YY, Nagai K
  • Journal Title: J Biosci Bioengineering 105(29) Pages: 164-167
  • Peer Reviewed
• [Journal Article] Transthyretin Accelerates Vascular Abeta Deposition in a Mouse Model of Alzheimer's Disease (2008)
  • Author(s): Wati H, Kawarabayashi T, Matsubara E, Kasai A, Hirasawa T, Kubota T, Hagiwara Y, Shogi M, Maeda S.
  • Journal Title: Brain Pathol 19(1) Pages: 48-57
  • Peer Reviewed
• [Journal Article] Augmentation of plysialic acid by valproic acid in early postnatal mouse hippocampus and primary cultured hippocampal neurons (2008)
  • Author(s): Natori T, Kodaira F, Hirasawa T, Gao YY, Nagai K.
  • Journal Title: J Biosci Bioengineering 105(29) Pages: 164-167
  • Peer Reviewed
• [Journal Article] ミクログリア可視化トランスジェニックマウスの作製 (2007)
  • Author(s): 平澤孝枝, 高坂新一
  • Journal Title: BRAIN AND NERVE 59(7) Pages: 763-772
• [Journal Article] Direct interaction of PDZ domain-containing synaptic molecule Shank3 with GluR1 AMPA receptor J (2006)
  • Author(s): Uchino S, Wada H, Honda S, Nakamura Y, Ondo Y,Uchiyama T, Tsutsumi M, Suzuki E, Hirasawa T, Kohsaka S
  • Journal Title: Neurochem 97(4) Pages: 1203-14
  • Peer Reviewed
• [Journal Article] 知的発達障害とエピジェネティクスの分子遺伝学 (2006)
  • Author(s): 平澤孝枝, 久保田健夫
  • Journal Title: 神経研究の進歩 50(5) Pages: 770-779
• [Presentation] NMDA receptors participate in neuronal migration in the early stage of mouse cortical development (2006)
  • Author(s): Takae Hirasawa, Hidenori Tabata, Kazunori Nakajima, Takeo Kubota, Shigeo Uchino, Shinichi Kohsaka
  • Organizer: 第49回 日本神経化学学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-13