| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
The human SIM2 gene is a gene that is located in chromosome 21q22.2 in Down syndrome chromosome region and the SIM2 protein is a transcription factor which belong to bHLH-PAS family. SIM2 protein act when generate dimer with transcription factors which are ARNT and ARNT2. Dimerization domain of SIM2 protein with ARNT protein has not been done in detail, so we had analyzed the SIM2-ARNT and SIM2-ARNT2 dimerization domain using deletion mutants for each functional domain in the SIM2 protein. After all, there are different dimerization domain in SIM2 protein required by partner proteins. Since there are two SIM2 protein that is differenciated by splicing, we have conducted analysis to identify how the function of SIM2 gene expression is different. Promoter assay and gel retardation assay showd that SIM2l and SIM2s seems to be regulated by different transcription factors.
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