The role of interleukin-16 and regulatory T cell in contact hypersensitivity.

• Project/Area Number: 18790799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MASUDA Koji Kyoto Prefectural University of Medicine, 医学研究科, 助教 (00326232)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,070,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: IL-16 / 接触過敏反応 / 制御性T細胞 / 制御・性T細胞

Research Abstract

BALB/cマウスに、ハプテンを塗布して用いて接触過敏反応(CHS)をマウスの耳介に惹起させその厚さを測定するとともに、惹起相で炎症局所に浸潤するリンパ球のフェノタイプ(CD4,CD8,CD25)の経時的な変化を免疫組織化学的に解析した。CD25陽性細胞は6時間後より認められたが24から72時間後にかけて増加傾向が認められた。一方、Foxp3mRNAの発現をrealtimeRT-PCRで測定したところ、その発現は惹起後6時間後より認められ、24時間後から72時間後にかけて強くなった。以上の経過よりCHSの惹起相における炎症反応の収束には制御性T細胞が関与していることが示唆された。Foxp3mRNAの発現の推移は耳介の厚さの変化に相関する傾向が見られた。またIL-16による制御性T細胞への影響は認められなかった。

Research Products

• [Journal Article] Platelet-derived microparticles and soluble P-selectin as platelet activation markers in patients with atopic dermatitis. (2009)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Masuda K, Kishimoto S.
  • Journal Title: Clin Immunol (in press)
  • Peer Reviewed
• [Journal Article] Erythema-multiforme-like eruption recurring in ultraviolet-exposed skin. (2008)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Masuda K, Kishimoto S.
  • Journal Title: Dermatology 216 Pages: 181-183
  • Peer Reviewed
• [Journal Article] A case of anaphylaxis to fish and shellfish. (2008)
  • Author(s): Masuda K, Katoh N, Fukuba K, Shimakura K, Kishimoto S.
  • Journal Title: J Dermatol 35 Pages: 181-182
  • Peer Reviewed
• [Journal Article] Elevated platelet activation in patients with atopic dermatitis and psoriasis: increased plasma levels of b-thrombogloblin and platelet factor 4 (2008)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Ueda E, Masuda K, Kishimoto S.
  • Journal Title: Allergol Int 57 Pages: 391-6
  • Peer Reviewed
• [Journal Article] Elevated platelet activation in patients with atopic dermatitis and psoriasis : increased plasma levels of b-thrombogloblin and platelet factor 4 (2008)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Masuda K. Kishimoto S.
  • Journal Title: Allergol Int 57 Pages: 391-396
  • Peer Reviewed
• [Journal Article] Erythema-multiforme-like eruption recurring om ultraviolet-exposed skin (2008)
  • Author(s): Tamagawa-Mineoka R, Katoh N, Masuda K, Kishimoto S.
  • Journal Title: Dermatology 216 Pages: 181-183
  • Peer Reviewed
• [Journal Article] A case of anaphylaxis to fish and shellfish (2008)
  • Author(s): Masuda K, Katoh N, Fukuba K, Shimakura K, Kishimoto S.
  • Journal Title: J Dermatol 35 Pages: 181-182
  • Peer Reviewed
• [Journal Article] Anaphylaxis to vitamin B2 added to an energy drink.
  • Author(s): Masuda K, Katoh N, Mizutani H, Kishimoto S.
  • Journal Title: Clin Exp Dermatol (in press)
  • Peer Reviewed
• [Presentation] The expression of Foxp3+ T cells in the elicitation phase of murine contact hypersensitivity (2007)
  • Author(s): Masuda K.
  • Organizer: 68th annual meeting of Society for Investigative Dermatology
  • Place of Presentation: Los Angeles,Carifornia,USA
  • Year and Date: 2007-05-07
• [Presentation] The expression of Foxp3+ T cells in the elicitation phase of murine contact hypersensitivity. (2007)
  • Author(s): Masuda K, Katoh N, Tamagawa-Mineoka R, Soga F, Kishimoto S.
  • Organizer: 68th annual meeting of Society for Investigative Dermatology
  • Place of Presentation: Los Angeles, California, USA
• [Presentation] Topical P2Y12 purinoreceptor antagonists reduce inflammation in irritant and allergic contact dermatitis models. (2007)
  • Author(s): Katoh N, Tamagawa-Mineoka R, Nara T, Soga F, Asai J, Masuda K, Kishimoto S.
  • Organizer: 68th annual meeting of Society for Investigative Dermatology
  • Place of Presentation: Los Angeles, California, USA