Experimental study for fusion vaccine of dendritic cell/ stromal cell/tumor cell transfected with LIGHT

• Project/Area Number: 18790960
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kobe University
• Principal Investigator: NAKAMURA Tetsu Kobe University, 医学部附属病院, 助教 (10403247)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,740,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: LIGHT / 癌ワクチン / LTβR / 樹状細胞 / 腫瘍細胞

Research Abstract

マウス腫瘍細胞の細胞膜上におけるLIGHTの発現は、今回の検討では困難であったが、COS7細胞におけるLIGHT発現の検討では細胞質内での発現は認めた。原因としてC末端におけるFLAG修飾が起因している可能性があり今後発現ベクターの改良により細胞膜上に機能的なLIGHT発現させることが可能と考える。

Research Products

• [Journal Article] Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma (2007)
  • Author(s): Yasuda T, Kamigaki T, Kawasaki K, Nakamura T, Yamamoto M, Kanemitsu K, Takase S, Kuroda D, Kim Y, Ajiki T, Kuroda Y
  • Journal Title: Cancer Immunol Immunother 56 Pages: 1025-1036
  • Peer Reviewed
• [Journal Article] Dendritic cell-tumor cell hybrids enhance the induction of cytotoxic T lymphocytes against murine colon cancer : a comparative analysis of antigen loading methods for the vaccination of immunotherapeutic dendritic cells. (2006)
  • Author(s): Yasuda T
  • Journal Title: Oncology Report 第16巻・第6号 Pages: 1317-1324
• [Journal Article] Correlation between expression of major histocompatibility complex class I and that of antigen presenting machineries in carcinoma cell lines of the pancreas, biliary tract and colon. (2006)
  • Author(s): Imanishi T
  • Journal Title: Kobe Journal of Medical Science 第52巻・第3-4号 Pages: 85-95
• [Journal Article] Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma.
  • Author(s): Yasuda T
  • Journal Title: Cancer Immunology, Immunotherapy (in press)
• [Presentation] The anti-tumor efficacy by regulatory T cells depletion in addition to DC vaccines for murine pancreatic cancer (2008)
  • Author(s): M Yamamoto, T Kamigaki, Y Iwatani, T Nakamura, Y Kuroda
  • Organizer: 第67回日本癌学会学術総会
  • Year and Date: 2008-10-29
• [Presentation] The anti-tumor efficacy by regulatory T cells depletion in addition to DC vaccines for murine pancreatic cancer (2008)
  • Author(s): M Yamamoto, T Kamigaki, Y Iwatani, T Nakamura, et.al.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-29