Study of HMGB protein : development of therapy and therapeutic drugs for ALI/ARDS

• Project/Area Number: 18791095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: UENO Hiroshi Kyoto Prefectural University of Medicine, 医学研究科, 助教 (20381965)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,970,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: HMGB蛋白 / 急性肺損傷 / ARDS / 抗HMGB抗体 / ATP / P2Y受容体 / 抗HMGB1抗体 / 抗HMGTB1抗体

Research Abstract

HMGBI、HMGB2の様々なエピトープを認識するモノクローナル抗体を急性肺傷害モデルマウスに気管内投与すると、HMGB1とHMGB2の相同性が最も低い部位をエピトープとする抗体が肺傷害軽減に最も効果的であり、HMGB2に特異性が高い抗体ほど肺傷害軽減効果は低い。また、健常マウスにHMGB抗体を投与すると、ほとんどのマウスが死亡することより、HMGB蛋白は生理的に重要な役割をしていると推察されたが、その役割までは解明できなかった。

Research Products

• [Journal Article] Acute lung inflammation and ventilator-induced lung injury caused by ATP via the P2Y receptors: an experimental study. (2008)
  • Author(s): Hiroki Matsuyama, Fumimasa Amaya, Soshi Hashimoto, Hiroshi Ueno, Satoru Beppu, Mitsuhiko Mizuta, Nobuaki Shime, Akitoshi Ishizaka and Satoru Hashimoto.
  • Journal Title: Respiratory Research 78 Pages: 9-9
  • Peer Reviewed
• [Journal Article] Dysregulation of lung injury and repair in moesin-deficient mice treated with intratracheal bleomycin. (2008)
  • Author(s): Soshi Hashimoto, Fumimasa Amaya, Hiroki Matsuyama, Hiroshi Ueno, Shojiro Kikuchi, Masaki Tanaka, Yoshihisa Watanabe, Masahito Ebina, Akitoshi Ishizaka, Sachiko Tsukita, and Satoru Hashimoto.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 295 Pages: 566-574
  • Peer Reviewed
• [Journal Article] Acute lung inflammation and ventilator-induced lung injury caused by ATP via the P2Y receptors : an experimental study (2008)
  • Author(s): Hiroki Matsuyama, Fumimasa Amaya, Soshi Hashimoto, Hiroshi Ueno, Satoru Beppu, Mitsuhiko Mizuta, Nobuaki Shime, Akitoshi Ishizaka and Satoru Hashimoto
  • Journal Title: Respiratory Research 79 Pages: 9-9
  • Peer Reviewed
• [Journal Article] Dysregulation of lung injury and repair in moesin-deficient mice treated with intratracheal bleomycin (2008)
  • Author(s): Soshi Hashimoto, Fumimasa Amaya, Hiroki Matsuyama, Hiroshi Ueno, Shojiro Kikuchi, Masaki Tanaka, Yoshihisa Watanabe, Masahito Ebina, Akitoshi Ishizaka, Sachiko Tsukita, and Satoru Hashimoto
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 295 Pages: 566-574
  • Peer Reviewed
• [Journal Article] エンドトキシン投与急性肺損傷マウスモデルにおける抗HMGB1抗体の効果. (2006)
  • Author(s): 上野博司、松田知之、橋本悟、天谷文昌、北村佳博、黄英文、副島潤子、山田晋吾、石坂彰敏、丸山征郎
  • Journal Title: 第9回Acute Lung Injury研究会講演 Pages: 42-44
• [Presentation] 人工換気による肺損傷発生における細胞外ATPの関与 (2008)
  • Author(s): 松山広樹, 天谷文昌, 橋本壮志, 上野博司, 橋本悟
  • Organizer: 第55回日本麻酔科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-12
• [Presentation] 人工換気による肺損傷発生における細胞外ATPの関与 (2008)
  • Author(s): 松山広樹、上野博司、橋本悟, 他
  • Organizer: 日本麻酔科学会第55回学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-06-12