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免疫性精子形成障害における環境因子の影響

Research Project

Project/Area Number 18791136
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionTokyo Medical University

Principal Investigator

内藤 宗和  Tokyo Medical University, 医学部, 助教 (10384984)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords精巣 / 精子形成 / 重金属 / 精巣血液関門 / 自己免疫 / 炎症 / マウス / 毒性
Research Abstract

前年度、フタル酸ジ-2-エチルヘキシル(DEHP)と塩化カドミウム(CdCl2)を雄マウスに投与し、精子形成障害は惹起されないが精巣一血液関門の微小構造変化が起こる用量を報告した。今回、光学顕微鏡で精子形成障害が観察されない用量であるDEHP(0.1%DEHP食餌)とCdCl_2(3mg/kg腹腔内投与)を雄マウスに投与し、精巣の免疫環境に与える影響を調べた。DEHP投与群は1週間後、2週間後に、CdCl_2投与群は12時間、72時間後にそれぞれ屠殺し、取り出した精巣の微細構造変化を免疫組織化学法{Horseradish peroxidase (HRP)、Immunoglobulin G (IgG)、過酸化脂質染色}、サイトカイン動態をReal-timePCR法を用いて調べた。その結果、CdCl_2投与群ではHRPやIgGが精細管の基底膜を越えて上皮細胞または上皮細胞間に流入する像が見られたが、DEHP群では見られなかった。過酸化脂質染色ではDEHP群で陽性反応が見られるものの、CdCl_2群では見られなかった。また、Real-timePCR法では両群でIL-6のみが優位に上昇した。これらの結果から、環境毒性物質は精子形成障害が観察されない容量においても精巣の免疫環境に影響を与えることが分かった。

Report

(2 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (9 results)

All 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Patterns of Infiltration of Lymphocytes into the Testis Under Normal and Pathological Conditions in Mice2008

    • Author(s)
      Munekazu, Naito
    • Journal Title

      American Journal of Reproductive Immunology 59

      Pages: 55-61

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Splenic cytokines in mice immunized with testicular germ cells2008

    • Author(s)
      Yo, Tokunaga
    • Journal Title

      International Journal of Andrology (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caput epididymitis but not orchitis was induced by vasectomy in a murine model of experimental autoimmune orchitis2008

    • Author(s)
      Ning, Qu
    • Journal Title

      Reproduction (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] カドミウム微量投与による精巣の免疫学的組織環境の変化2007

    • Author(s)
      小川夕輝
    • Journal Title

      Reproductive Immunology and Biology 22

      Pages: 49-55

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunomorphological aspects of the tubuli recti and the surrounding interstitium in normal mice.2007

    • Author(s)
      Takahashi K, Naito M, Terayama H, Qu N, Cheng L, Tainosho S, Itoh M
    • Journal Title

      International Journal of Andrology 30 (1)

      Pages: 21-27

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Short term effects of di-(2-ethylhexyl) phthalate (DEHP) on testes, liver, kidneys and pancreas in mice.2007

    • Author(s)
      Miura Y, Naito M, Ablake M, Terayama H, Shuang-Qin Yi, Qu N, Cheng L, Suna S, Jitsunari F, Itoh M
    • Journal Title

      Asian Journal of Andrology 9 (2)

      Pages: 199-205

    • Related Report
      2006 Annual Research Report
  • [Presentation] Distribution of blood and lymphoid vessels in mouse epididymis2008

    • Author(s)
      内藤宗和
    • Organizer
      第113回 日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分大学医学部
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Humoral immunity during experimental autoimmune orchitis2007

    • Author(s)
      Munekazu, Naito
    • Organizer
      27th Annual Meeting of the American Sciety for Reproductive Immunology
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2007-05-15
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.tokyo-med.ac.jp/anatomy/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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