The analysis of estrogen/progesterone responding genes in GnRH neuronal cells

• Project/Area Number: 18791166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Yokohama City University
• Principal Investigator: YOSHIDA Hiroshi Yokohama City University, 市民総合医療センター・婦人科, 助教 (50405007)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 生殖医学

Research Abstract

GT1細胞を用いたマイクロアレイ法にてエストロゲンまたは・およびプロゲステロンにより調節されうる遺伝子をスクリーニングした。その中でNO synthetase 3に着目しGT1細胞内での発現パターンと分泌調節へのかかわりについて検討した。さらにcalcium sensing receptorの豊富な発現を認め、同様にその発現パターンと分泌調節への関係について検討した。

Research Products

• [Journal Article] Weiner Frequency of intrinsic pulsatile secretion is regulated by the expression of cyclic nucleotide gated channels in GT1 cells (2007)
  • Author(s): B. E. Blackman, H. Yoshida, S. Paruthiyil and R. I.
  • Journal Title: Endocrinology 148(7) Pages: 3299-3306
  • Peer Reviewed
• [Journal Article] Frequency of intrinsic pulsatile secretion is regulated by the expression of cyclic nucleotide gated channels in GT1 cells (2007)
  • Author(s): B.E.Blackman, H.Yoshida, S.Paruthivil and R.I.Weiner
  • Journal Title: Endocrinology 148(7) Pages: 3299-306
  • Peer Reviewed
• [Presentation] マウス妊娠子宮におけるカルシウムセンシングレセプターの発現 (2007)
  • Author(s): 吉田浩、神田義明、榊原秀也、平原史樹
  • Organizer: 日本産科婦人科学会学術集会
  • Place of Presentation: 京都
• [Presentation] Fumiki Hirahara Regulation of GnRH secretion from immortalized GnRH Neuronal Cell Line by Calcium Sensing Receptor (2006)
  • Author(s): Hiroshi Yoshida, Makiko Koyama, Hideya Sakakibara
  • Organizer: Annual meeting of American Endocrine Society
  • Place of Presentation: Boston