Regeneration of extracellular matrix of corneal stroma using corneal stroma-derived progenitor cells

• Project/Area Number: 18791302
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tokyo Dental College
• Principal Investigator: YOSHIDA Satoru Tokyo Dental College, 歯学部, 客員講師 (50398781)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 角膜実質 / ケラトサイト / 体性幹細胞 / 神経堤幹細胞 / 人工角膜 / 角膜内皮 / 神経堤

Research Abstract

生体組織に近い人工角膜構築のためには、組織にもともと存在する細胞を用いることが望ましく、また、恒常的に安定な細胞の供給のためは組織幹細胞の分離・培養法の確立が必要である。我々は実験動物(マウス)を用いて、角膜実質内に組織間細胞が存在している事を明らかにし、また、その培養法を確立した。

Research Products

• [Journal Article] The use of human mesenchymal stem cell-derived feeder cells for the cultivation of transplantable epithelial sheets. (2009)
  • Author(s): Omoto M, Miyashita H, Shimmura S, Higa K, Kawakita T, Yoshida S, McGrogan M,Shimazaki J, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci. 50(5) Pages: 2109-15
  • Peer Reviewed
• [Journal Article] Isolation of oral epithelial progenitors using collagen IV. (2008)
  • Author(s): Igarashi T, Shimmura S, Yoshida S, Tonogi M, Shinozaki N, Yamane GY
  • Journal Title: Oral Dis. 14(5) Pages: 413-8
  • Peer Reviewed
• [Journal Article] A novel NIH/3T3 duplex feeder system to engineer corneal epithelial sheets with enhanced cytokeratin 15-positive progenitor populations. (2008)
  • Author(s): Miyashita H, Shimmura S, Higa K, Yoshida S, Kawakita T, Shimazaki J, Tsubota K
  • Journal Title: Tissue Eng Part A. 14(7) Pages: 1275-82
  • Peer Reviewed
• [Journal Article] 角膜に存在する神経堤幹細胞 (2008)
  • Author(s): 吉田悟
  • Journal Title: 実験医学増刊 26 Pages: 113-117
• [Journal Article] Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces. (2006)
  • Author(s): Yoshida S, Shimmura S, Kawakita T, Miyashita H, Den S, Shimazaki J, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci. 47(11) Pages: 4780-6
  • Peer Reviewed
• [Journal Article] Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea. (2006)
  • Author(s): Yoshida S, Shimmura S, Nagoshi N, Fukuda K, Matsuzaki Y, Okano H, Tsubota K
  • Journal Title: Stem Cells 24(12) Pages: 2714-22
  • Peer Reviewed
• [Journal Article] Proteomic analysis of soluble factors secreted by limbal fibroblasts. (2006)
  • Author(s): Shimmura S, Miyashita H, Higa K, Yoshida S, Shimazaki J, Tsubota K
  • Journal Title: Mol Vis 12 Pages: 478-84
  • Peer Reviewed
• [Journal Article] Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea. (2006)
  • Author(s): 吉田悟
  • Journal Title: Invest Ophthalmol Vis Sci. 47 (11) Pages: 4780-4786
• [Presentation] マウス角膜実質からの神経堤由来の多分可能を有した幹細胞の分離 (2008)
  • Author(s): 吉田 悟
  • Organizer: 第23回内藤コンファレンス
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 2008-11-13
• [Presentation] 角膜実質に存在する神経提由来幹細胞 (2008)
  • Author(s): 吉田 悟
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-13
• [Presentation] Expression patterns of Nucleostemin (Gnl3) in mouse and human cornea (2008)
  • Author(s): Yoshida S
  • Organizer: The Association for Research in Vision and Ophthalmology, Annual Meeting
  • Place of Presentation: Florida, U.S.A.
  • Year and Date: 2008-04-29
• [Presentation] マウス及びヒト角膜におけるNucleostemin (Gnl3) の発現 (2008)
  • Author(s): 吉田 悟
  • Organizer: 第32回角膜カンファランス
  • Place of Presentation: 東京ベイホテル東急
  • Year and Date: 2008-02-29
• [Presentation] マウス及びヒト角膜におけるNucleostemin(Gn13)の発現 (2008)
  • Author(s): 吉田悟
  • Organizer: 第32回角膜カンファランス
  • Place of Presentation: 東京ベイホテル東急
  • Year and Date: 2008-02-29
• [Presentation] マウス角膜実質からの神経堤由来の多分可能を有した幹細胞の分離 (2007)
  • Author(s): 吉田 悟
  • Organizer: 第6回日本再生医療学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-03-13
• [Presentation] 輪部基底細胞におけるサイトケラチン15の発現 (2007)
  • Author(s): 吉田 悟
  • Organizer: 第31回角膜カンファランス
  • Place of Presentation: ワールドコンベンションセンターサミット(宮崎市)
  • Year and Date: 2007-02-09
• [Presentation] Multipotent cornea stromal precursors are of neural crest origin and not the bone marrow (2006)
  • Author(s): Yoshida S
  • Organizer: The Association for Research in Vision and Ophthalmology, Annual Meeting
  • Place of Presentation: Florida, U.S.A.
  • Year and Date: 2006-04-30
• [Book] 実験医学増刊、角膜に存在する神経堤幹細胞 (2008)
  • Author(s): 吉田悟、榛村重人
  • Publisher: 羊土社