Effect of axonal supercharging using reverse end-to-side neurorrhaphy in peripheral nerve repair in the rat model

• Project/Area Number: 18791321
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Osaka University
• Principal Investigator: FUJIWARA Toshihiro Osaka University, 医学部附属病院, 医員 (00423179)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 神経再生 / 顔面神経 / 坐骨神経モデル / 神経端側縫合 / 末梢神経再生 / OMgP / 神経軸索ガイダンス因子 / Slit / 端側縫合 / 神経二重支配 / 坐骨神経

Research Abstract

神経の損傷部位より末梢側の側面に他の神経を端側縫合付加することによる機能回復促進効果をラット坐骨神経モデルを用いて検討した。モデルとして、右の坐骨神経を切断し、端端縫合にて修復したI群と右の坐骨神経切断後の神経端端縫合に加え、更に左側の坐骨神経を右の坐骨神経に端側縫合付加したII群を用意し、術後3ヵ月後に機能回復の程度を比較、調査した。結果、端端縫合部より末梢側に他の神経を端側縫合付加することで、その支配筋ならびに知覚の回復が促進された。そして、神経端側縫合付加したII群の方が端側縫合部より末梢側において神経再生が促進されていた。また神経端側縫合付加したII群において目的の筋組織は両側の坐骨神経により二重支配されていた。神経端側縫合付加することで支配組織の機能回復が促進された理由として、支配組織への再生軸索数の増加や支配組織への早期からの神経再支配による筋組織の萎縮の軽減が考えられた。このことから末梢神経損傷後の支配組織の機能回復を促進する方法として別の神経を用いた神経端側縫合付加は有用であると思われた。

Research Products

• [Journal Article] 【神経端側縫合】神経端側縫合の基礎研究 端側縫合を用いた神経移植とaxonal supercharging techniqueへの応用 (2008)
  • Author(s): 久保盾貴, 松田健, 藤原敏宏, 冨田興一, 矢野健二, 細川亙
  • Journal Title: 日本マイクロサージャリー学会会誌 21巻3号 Pages: 249-255
  • Peer Reviewed
• [Journal Article] Reduction of oligodendrocyte myelin glycoprotein expression following facial nerve transection (2008)
  • Author(s): Koyama Yoshihisa, Fujiwara Toshihiro, Kubo Tateki, Tomita Koichi, Yano Kenji, Hosokawa Ko, Tohyama Masaya
  • Journal Title: Journal of Chemical Neuroanatomy 36(3-4) Pages: 209-215
  • Peer Reviewed
• [Journal Article] mRNA expression changes of slit proteins following peripheral nerve injury in the rat model (2008)
  • Author(s): Fujiwara Toshihiro, Kubo Tateki, Koyama Yoshihisa, Tomita Koichi, Yano Kenji, Tohyama Masaya, Hosokawa Ko
  • Journal Title: Journal of Chemical Neuroanatomy 36(3-4) Pages: 170-176
  • Peer Reviewed
• [Journal Article] A new model of end-to-side nerve graft for multiple branch reconstruction : end-to-side cross-face nerve graft in rats (2008)
  • Author(s): Matsuda Ken, Kakibuchi Masao, Kubo Tateki, Tomita Koichi, Fujiwara Toshihiro, Hattori Ryo, Yano Kenji, Hosokawa Ko
  • Journal Title: Journal of Plastic, Reconstructive & Aesthetic Surgery 61(11) Pages: 1357-1367
  • Peer Reviewed
• [Journal Article] mRNA expression changes of slit proteins following peripheral nerve injury in the rat model (2008)
  • Author(s): Toshihiro Fujiwara, et.al.
  • Journal Title: Journal of Chemical Neuroanatomy 36(3-4) Pages: 170-176
  • Peer Reviewed
• [Journal Article] Reduction of oligodendrocyte myelin glycoprotein expression following facial nerve transection (2008)
  • Author(s): Yoshihisa Koyama, Toshihiro Fujiwara, et.al.
  • Journal Title: Journal of Chemical Neuroanatomy 36(3-4) Pages: 209-215
  • Peer Reviewed
• [Journal Article] Superior Orbital Fissure Syndrome After Repairof Maxillary and Naso-orbito-ethmoid Fractures : A Case Study (2008)
  • Author(s): Toshihiro Fujiwara, et.al.
  • Journal Title: Journal of Plastic Reconstructive and Aesthetic Surgery (in press)
  • Peer Reviewed
• [Journal Article] Axonal supercharging technique using reverse end-to-side neurorrhaphy in peripheral nerve repair : an experimental study in the rat model (2007)
  • Author(s): Fujiwara Toshihiro, Matsuda Ken, Kubo Tateki, Tomita Koichi, Hattori Ryo, Masuoka Takeshi, Yano Kenji, Hosokawa Ko
  • Journal Title: Journal of Neurosurgery 107(4) Pages: 821-829
  • Peer Reviewed
• [Journal Article] The neurotrophin receptor p75NTR in Schwann cells is implicated in remyelination and motor recovery after peripheral nerve injury (2007)
  • Author(s): Tomita Koichi, Kubo Tateki, Matsuda Ken, Fujiwara Toshihiro, Yano Kenji, Winograd Jonathan M, Tohyama Masaya, Hosokawa Ko
  • Journal Title: Glia 15;55(11) Pages: 1199-1208
  • Peer Reviewed
• [Journal Article] Effect of conduit repair on aberrant motor axon growth within the nerve graft in rats (2007)
  • Author(s): Tomita Koichi, Kubo Tateki, Matsuda Ken, Hattori Ryo, Fujiwara Toshihiro, Yano Kenji, Hosokawa Ko
  • Journal Title: Microsurgery 27(5) Pages: 500-509
  • Peer Reviewed
• [Journal Article] Nerve bypass grafting for the treatment of neuroma-in-continuity : an experimental study on the rat (2007)
  • Author(s): Tomita Koichi, Kubo Tateki, Matsuda Ken, Fujiwara Toshihiro, Kawai Kenichiro, Masuoka Takeshi, Yano Kenji, Hosokawa Ko
  • Journal Title: Journal of Reconstructive Microsurgery 23(3) Pages: 163-171
  • Peer Reviewed
• [Journal Article] Axonal supercharging technique using reverse end-to-side neurotrhappy in peripheral nerve repair:an expaimental study in the rat model (2007)
  • Author(s): Tbshihiro Fujiwara
  • Journal Title: Journal of Neiirosurgery 107 Pages: 821-829
  • Peer Reviewed
• [Journal Article] Superior orbital fissure syndrome after repair of maxillary and naso-orbito-ethmoid fractures : a case report
  • Author(s): Fujiwara Toshihiro, Matsuda Ken, Kubo Tateki, Tomita Koichi, Yano Kenji, Hosokawa Ko
  • Journal Title: Journal of Plastic, Reconstructive & Aesthetic Surgery (in pressa)
  • Peer Reviewed
• [Presentation] ラット顔面神経切断モデルを用いたOMgpの発現の解析 (2008)
  • Author(s): 藤原敏宏
  • Organizer: 第17回日本形成外科学会基礎学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2008-10-02
• [Presentation] ラット顔面神経切断モデルを用いたOMgpの発現解析 (2008)
  • Author(s): 藤原敏宏, 久保盾貴, 冨田興一, 矢野健二, 細川亙
  • Organizer: 第17回日本形成外科学会基礎学術集会
  • Place of Presentation: 東京
• [Presentation] 上眼窩裂症候群の危険因子としての上眼窩裂幅の解剖学的検討 (2008)
  • Author(s): 藤原敏宏, 久保盾貴, 菊池守, 冨田興一, 高田章好, 矢野健二, 細川亙
  • Organizer: 第51回日本形成外科総会・学術集会
  • Place of Presentation: 愛知
• [Presentation] ラット顔面神経切断モデルを用いたSlit family mRNA発現の解析 (2007)
  • Author(s): 藤原 敏宏
  • Organizer: 第16回日本形成外科学会基礎学術集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-10-12
• [Presentation] 服部ラット顔面神経切断モデルを用いたSlit family mRNA発現の解析 (2007)
  • Author(s): 藤原敏宏, 久保盾貴, 冨田興一
  • Organizer: 第16回日本形成外科学会基礎学術集会
  • Place of Presentation: 兵庫
• [Presentation] 端側神経縫合付加は末梢神経損傷後の機能回復を促進する (2006)
  • Author(s): 藤原敏宏, 松田健, 久保盾貴, 冨田興一, 服部亮, 升岡健, 矢野健二, 細川亙
  • Organizer: 第15回日本形成外科学会基礎学術集会
  • Place of Presentation: 埼玉